scholarly journals Green Synthesis, Characterization and Antimicrobial activity of Silver Nanoparticles from the Extract of Lagerstroemia speciosa

2021 ◽  
Vol 37 (3) ◽  
pp. 648-655
Author(s):  
Vinit Prakash ◽  
Harpreet Kaur ◽  
Anjana Kumari ◽  
Manoj Kumar ◽  
Sumeet Gupta ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Antimicrobial Activity ◽  
Silver Nanoparticles ◽  
Green Synthesis ◽  
Kidney Diseases ◽  
Ethanolic Extract ◽  
Antimicrobial Activities ◽  
Diffusion Method ◽  
Bacterial Strains ◽  
Ag Nps

Lagerstroemia speciosais commonly known as Banaba or Jarul which is used to get rid of various ailments such as fever, urinary infection, decongestion, diarrhoea, mouth ulcers, astringent, diabetes mellitus, kidney diseases, abdominal pains etc. The present work, describes the green synthesis of silver nanoparticles from ethanolic extract of fruits of L.speciosa (Ls-Ag NPs) and their analysis for antimicrobial activities. The characterisation of so obtained nanoparticles have been carried out with help of Field emission scanning electron microscopy (FESEM) and High-resolution transmission electron microscopy (HRTEM). Further, antimicrobial activities of ethanolic extract of fruits of L.speciosa(Ee-Ls), silver oxide (Ag2O), and Ls-Ag NPs have been examined by using well-diffusion method against two bacterial strains: Staphylococcus aureus (gram-positive) and Escherichia coli (gram-negative) and one fungal strain: Aspergillus niger.It has been observed that the biosynthesized Ls-Ag NPs possess much effective antimicrobial activity against selected strains as compared to Ee-Ls and Ag2O.

scholarly journals Characterization and Antibacterial Response of Silver Nanoparticles Biosynthesized Using an Ethanolic Extract of Coccinia indica Leaves

Crystals ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 97
Author(s):  
Suresh V. Chinni ◽  
Subash C. B. Gopinath ◽  
Periasamy Anbu ◽  
Neeraj Kumar Fuloria ◽  
Shivkanya Fuloria ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Antimicrobial Activity ◽  
Silver Nanoparticles ◽  
Zeta Potential ◽  
Ethanolic Extract ◽  
Spherical Shape ◽  
Bacterial Strains ◽  
X Ray ◽  
Coccinia Indica ◽  
Antibacterial Potential

The present study was planned to characterize and analyze the antimicrobial activity of silver nanoparticles (AgNP) biosynthesized using a Coccinia indica leaf (CIL) ethanolic extract. The present study included the preparation of CIL ethanolic extract using the maceration process, which was further used for AgNP biosynthesis by silver nitrate reduction. Biosynthetic AgNPs were characterized using UV–Visible spectrometry, zeta potential analysis, transmission electron microscopy (TEM), scanning electron microscopy (SEM), atomic force microscopy (AFM), X-ray diffraction (XRD), and energy-dispersive X-ray (EDX) spectrometry. The biogenic AgNP and CIL extracts were further investigated against different bacterial strains for their antimicrobial activity. The surface plasmon resonance (SPR) signal at 425 nm confirmed AgNP formation. The SEM and TEM data revealed the spherical shape of biogenic AgNPs and size in the range of 8 to 48 nm. The EDX results verified the presence of Ag. The AgNPs displayed a zeta potential of −55.46 mV, suggesting mild AgNP stability. Compared to Gram-positive bacteria, the biogenic AgNPs demonstrated high antibacterial potential against Gram-negative bacteria. Based on the results, the current study concluded that AgNPs based on CIL extract have strong antibacterial potential, and it established that AgNP biosynthesis using CIL ethanol extract is an effective process.

scholarly journals A Comparative Study of Antimicrobial Activity of Ethanolic Extract of Tecoma stans and Abutilon indicum Leaves: An In vitro Study

2021 ◽  
pp. 609-615
Author(s):  
M. Binigha ◽  
R. Gayatri Devi ◽  
J. Selavaraj ◽  
A. Jothi Priya
Keyword(s):  
Antimicrobial Activity ◽  
Agar Plate ◽  
Ethanolic Extract ◽  
Flowering Plant ◽  
Diffusion Method ◽  
Bacterial Strains ◽  
Zone Of Inhibition ◽  
The Family ◽  
Abutilon Indicum

Tecoma stans is a flowering plant belonging to the family Bignoniaceae. It is the floral emblem of the Bahamas. Abutilon indicum belongs to the family Malvaceae. It is also called Indian Mallow. Tecoma stans and Abutilon indicumhave antimicrobial activity and can be used in producing antibiotics. There is an urgent need to produce new antibiotics as there is an increase in the development of pathogen resistant drugs. The aim of this study was to compare the antimicrobial activity of ethanolic extract of Tecoma stansand Abutilonindicum leaves. E.faecalis bacteria were maintained in nutrient agar slopes. The powder of the two herbs, were subjected to extraction by Soxhlet extractor and was concentrated to dry residue by Agar well diffusion method. The nutrient broth was inoculated with bacterial strains. The culture was adjusted to 0.5McFarland turbidity standard. Lawn culture was done in Muller-Hinton agar plate and then the plates were dried. A 6 mm diameter well is bored for different concentrations. The extracts were introduced into the well and allowed to stand for 24 hrs. The antibacterial activity was determined by measuring the zone of inhibition. The present finding on the antimicrobial potential of Abutilon indicum and Tecoma stands showed that the two plants have growth inhibitory activity against oral pathogen E. faecalis. Abutilon indicum has a zone of inhibition greater than Tecoma stans. Abutilon indicum exhibited better activity compared to Tecoma stans.

scholarly journals Green Synthesis and Antibacterial Activity of HAp@Ag Nanocomposite Using Centella asiatica (L.) Urban Extract and Eggshell

2020 ◽  
Vol 2020 ◽  
pp. 1-12
Author(s):  
Xuan Nui Pham ◽  
Hoa Thi Nguyen ◽  
Ngan Thi Pham
Keyword(s):  
Electron Microscopy ◽  
Antibacterial Activity ◽  
Green Synthesis ◽  
Antibacterial Properties ◽  
Centella Asiatica ◽  
Diffusion Method ◽  
Ag Nps ◽  
X Ray ◽  
Synthesis Of Nanoparticles ◽  
Green Approach

In recent years, the green synthesis of nanoparticles via biological processes has attracted considerable attention. Herein, we introduce a facile and green approach for the synthesis of poriferous silver nanoparticles (Ag-NPs) decorated hydroxylapatite (HAp@Ag) nanoparticles with excellent antibacterial properties. All the nanocomposites were fully characterized in the solid state via various techniques such as X-ray powder diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM), and energy-dispersive X-ray spectrometer (EDX), in which the synthesized Ag-NPs (24 nm in diameter) and their homogeneous incorporation on HAp have been studied by ultraviolet-visible (UV-vis) technique, transmission electron microscopy (TEM), and dynamic light scattering (DLS) analysis. The obtained results indicate that the structure and morphology of HAp have no significant changes after the incorporation of Ag-NPs on its surface. Moreover, an impressive antibacterial activity of HAp@Ag nanocomposite against Gram-positive bacterium Staphylococcus aureus and Gram-negative bacteria Escherichia coli and Pseudomonas aeruginosa has been recorded by using the agar well diffusion method. As a result, the HAp@Ag nanocomposite promises to be a great biomedical material with high antibacterial properties.

scholarly journals Green Synthesis, Characterization, and Evaluation of the Antimicrobial Activity of Camellia sinensis Silver Nanoparticles

2021 ◽  
Vol 2021 ◽  
pp. 1-7
Author(s):  
Toga Khalid Mohamed ◽  
Marivt Osman Widdatallah ◽  
Maida Musa Ali ◽  
Afraa Mubarak Alhaj ◽  
DhiaEldin AbdElmagied Elhag
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Antimicrobial Activity ◽  
Silver Nanoparticles ◽  
Green Synthesis ◽  
Camellia Sinensis ◽  
Multiple Drug Resistance ◽  
Visible Spectroscopy ◽  
X Ray ◽  
Scanning Electron

An extremely worrying and alarming increase in the level of multiple drug resistance is reported in Sudan, in which bacterial strains are becoming resistant to many commonly available antibiotics. Eventually, it is becoming extremely difficult to treat debilitating infections. In search of promising solutions to this arising crisis, Camellia sinensis silver nanoparticles were synthesized using the green synthesis method. The synthesis of the Camellia sinensis silver nanoparticles is confirmed using analytical methods as ultraviolet-visible spectroscopy, X-ray diffractometer, and scanning electron microscopy. Using the ultraviolet-visible spectroscopy, an absorption band of 412 nm was observed. Furthermore, scanning electron microscopy revealed the presence of silver nanoparticles which fell within the range of 1–100 nm, and X-ray diffractometer analysis showed three intense peaks with a maximum intense peak at 24.3 theta. Nanoparticles distribution between 12 nm and 64 nm was observed with an average diameter of 18.115 nm. It also revealed orthorhombic-shaped nanoparticles. The synthesized nanoparticles showed antimicrobial activity against Staphylococcus aureus with a zone of inhibition of 7 mm, but none was detected against Escherichia coli. The obtained physicochemical properties were correlated with the antibacterial activity of the silver nanoparticles.

Heteroaromatization with 4-Phenyldiazenyl-1-naphthol. Part IV: Synthesis of Some New Heterocyclic Compounds with Potential Biological Activity

2019 ◽  
Vol 16 (6) ◽  
pp. 931-938
Author(s):  
Hany M. Mohamed ◽  
Ashraf H.F. Abd El-Wahab
Keyword(s):  
Antimicrobial Activity ◽  
Agar Medium ◽  
Potassium Hydroxide Solution ◽  
Antimicrobial Activities ◽  
Azo Compounds ◽  
Diffusion Method ◽  
Bacterial Strains ◽  
Pharmacological Agents ◽  
Tetrazole Derivatives

Background: Synthetic azo compounds and their derivatives have been studied extensively due to their biological and pharmacological activities. Pyranopyridines, pyranopyrimidines and tetrazoles derivatives have emerged as a promising and attractive scaffold in the development of potent biological and pharmacological agents. Objectives: To design a series of new benzochromeno(pyridine/pyrimidine/tetrazole) derivatives and evaluate their antimicrobial activity against some bacterial strains (Gram-positive and Gram-negative) and some fungal strains. Materials and Methods: The (E)-7-(4-chlorophenyl)-5-(phenyldiazenyl)-10-thioxo-7,9,10,11-tetrahydro-8Hnaptho[ 1,2-b]pyrano[2,3-d]pyrimidin-8-one (4) was synthesized by the reaction of 4H-naphtho[1,2-b]pyran-3- carbonitrile (3) with carbon disulfide in alcoholic potassium hydroxide solution. Reaction of 3 with sodium azide in DMF and in presence of ammonium chloride afforded 6-(phenyldiazenyl)-3-(1H-tetrazol-5-yl)-4Hbenzo[ h]chromen-2-amine (7) while with malononitrile, thiourea or urea gave chromeno[2,3-b]pyridine-9- carbonitrile (8), chromeno[2,3-d]pyrimidine-10-thione (9) and chromeno[2,3-d]pyrimidin-10-one (10), respectively. The assignment structures were established on the basis of spectral data. Results: In this study, the antimicrobial activity of the synthesized compounds 3-12 was examined for their in vitro antimicrobial activity by using agar diffusion method such as Mueller-Hinton agar medium for bacteria and Sabouraud’s agar medium for fungi. Ampicillin and mycostatine were included in the experiments as reference drugs. Conclusion: A series of new benzochromeno(pyridine/pyrimidine/tetrazole) derivatives were synthesized in this work. All compounds were evaluated in antimicrobial activities.

Preliminary phytochemical screening and antimicrobial activities of plant extract of Elaeocarpus ganitrus Roxb.

2016 ◽  
Vol 5 (09) ◽  
pp. 4885 ◽  
Author(s):  
Khushbu Pandey ◽  
Mahendra Singh* ◽  
Bharat Pandey ◽  
Anshulika Upadhyaya ◽  
Kamal K. Pande
Keyword(s):  
Antimicrobial Activity ◽  
Rhizopus Oryzae ◽  
Antimicrobial Activities ◽  
Diffusion Method ◽  
Phytochemical Analysis ◽  
Phytochemical Screening ◽  
Bacterial Strains ◽  
E Coli ◽  
Antibacterial And Antifungal ◽  
Anti Fungal

The present study was carried out for phytochemical screening of principle bioactive compounds and antimicrobial activity in Elaeocarpus ganitrus Roxb., Phytochemical analysis revealed the presence of saponin, terpenoid, steroid, saponin, flavonoid, tannin and alkaloid. The petroleum, ether, chloroform, methanol, acetone and aqueous extracts were subjected to antimicrobial activity against bacterial strains Staphylococcus aureus, Pseudomonas, E. coli and Bacillus subtilis against anti-fungal strains A.awamori, A.fumigatus, Rhizopus oryzae, Trichoderma viridae and C.oryzae. The antibacterial and antifungal activity was evaluated by disc-diffusion method.

scholarly journals IN VITRO SCREENING OF ANTIMICROBIAL ACTIVITY OF ETHANOLIC EXTRACT OBTAINED FROM FICUS LYRATA WARB. (MORACEAE) LEAVES

2016 ◽  
Author(s):  
H. Tkachenko ◽  
L. Buyun ◽  
Z. Osadovskyy ◽  
M. Truhan ◽  
Ye. Sosnowski ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Pathogenic Bacteria ◽  
Antimicrobial Agents ◽  
Ethanolic Extract ◽  
Antimicrobial Activities ◽  
Diffusion Method ◽  
Traditional Use ◽  
Agar Disc ◽  
Ficus Lyrata

In the current investigation, screening of ethanolic extract obtained from Ficus lyrata leaves against pathogenic bacteria has been done in order to assess the antimicrobial activity aimed at detecting new sources of antimicrobial agents. The antimicrobial activity of the extract was determined using agar disc diffusion method. The antibacterial activity of leaf extract of F. lyrata was tested against human pathogenic bacteria — both Gram-positive (Staphylococcus aureus, methicillin-resistant S. aureus and Streptococcus pneumoniae) and Gram-negative strains (Klebsiella pneumoniae, Pseudomonas aeruginosa, and Escherichia coli). The results of this study provide evidence that the ethanolic extract of F. lyrata leaves has a mild antimicrobial activities, apparently, attributed to the presence of various secondary metabolites, which confirm the traditional use of this plant for the treatment of diseases caused by pathogens. These data allow us to suggest that the extracts of F. lyrata can be used to discover antibacterial substances for developing new pharmaceuticals to control clinically important pathogens responsible for severe disorders.

scholarly journals Optimization of antimicrobial efficiency of silver nanoparticles against three oral microorganisms in irreversible hydrocolloid impressions

2021 ◽  
Author(s):  
Ahmad Jafari ◽  
Ramin Mazaheri Nezhad Fard ◽  
Sima Shahabi ◽  
Farid Abbasi ◽  
Golshid Javdani Shahedin ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Silver Nanoparticles ◽  
Antimicrobial Agents ◽  
Diffusion Method ◽  
Ag Nps ◽  
Disk Diffusion Method ◽  
E Coli ◽  
Irreversible Hydrocolloid ◽  
Predicted Model ◽  
Water Ratio

Background and Objectives: Silver nanoparticles (Ag-NPs) are potent antimicrobial agents, which have recently been used in dentistry. The aim of the current study was to optimize antimicrobial activity of Ag-NPs used in preparing irre- versible hydrocolloid impressions against three microorganisms of Escherichia coli, Streptococcus mutans and Candida albicans. Materials and Methods: After assessing antimicrobial activity of the compound using disk diffusion method, three parame- ters of concentration of Ag-NPs (250-1000 ppm), ratio of hydrocolloid impression material powder to water (0.30-0.50) and time of mixing (20.0-60.0 s), affecting antimicrobial activity of irreversible hydrocolloid impression materials against the three microorganisms, were optimized. This combined process was successfully modeled and optimized using Box-Behnken design with response surface methodology (RSM). Decreases in colony number of E. coli, S. mutans and C. albicans were proposed as responses. Results: Qualitative antimicrobial assessments respectively showed average zone of inhibition (ZOI) of 3.7 mm for E. coli, 3.5 mm for S. mutans and 4 mm for C. albicans. For all responses, when the mixing duration and powder-to-water ratio increased, the circumstances (mixing duration of 59.38 s, powder-to-water ratio of 0.4 and Ag-NP concentration of 992 response) increased. Results showed that in optimum ppm, the proportion of decreases in colony numbers was maximum (89.03% for E. coli, 87.08% for S. mutans and 74.54% for C. albicans). Regression analysis illustrated a good fit of the ex- perimental data to the predicted model as high correlation coefficients validated that the predicted model was well fitted with data. Values of R2Adj with R2Pred were associated to the accuracy of this model in all responses. Conclusion: Disinfection efficiency dramatically increased with increasing of Ag-NP concentration, powder-to-water ratio and mixing time.

scholarly journals Antimicrobial activity of the biosynthesized silver nanoparticles of Gossypium hirsutum leaves extract

2021 ◽  
Vol 15 (3) ◽  
pp. 189-198
Author(s):  
Laxman S Vijapur ◽  
Y Srinivas ◽  
Anita R Desai ◽  
Jayadev N Hiremath ◽  
Channayya I Swami ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Particle Size ◽  
Silver Nanoparticles ◽  
Zeta Potential ◽  
Gossypium Hirsutum ◽  
Colour Change ◽  
Ethanolic Extract ◽  
Inhibition Zone ◽  
Nano Particles ◽  
Diffusion Method

Silver nanoparticles were successfully synthesized using silver nitrate and Gossypium hirsutum leaves extracts by varying the different concentration of aqueous & ethanolic extract. The Gossypium hirsutum leaves extract was containing phytoconstituents like carbohydrates, proteins, glycosides, flavonoids, alkaloids, tannins and phenolic compounds. Formation of Silver Nano Particles was primarily confirmed by colour change of yellow to brownish color. Silver nanoparticle with phytochemicals was confirmed by UV-Visible spectra by observing peak absorption of aqueous and ethanolic SNP at 430.0 nm and 416.0 nm respectively. Dynamic light scattering of the prepared formulations revealed all the formulation were within nano range, The particle size of SNP were in between 282.1nm to 205.7nm& zeta potential -38.66mV to -4.80mv for aqueous extract & particle size of SNP for ethanolic extract were in between 201.7nm to 156.1nm & zeta potential -29.95mV to -25.36mv.SEM showed the bio synthesized SNP were found to be spherical with rough surface & agglomerated. Antimicrobial activity of biosynthesized SNP was evaluated by means of inhibition zone analysis through well diffusion method, where SNP biosynthesized from aqueous and ethanolic extracts of G.hirsutum showed good antimicrobial activity against studied microorganisms.

scholarly journals ANTIMICROBIAL EVALUATION OF ETHANOLIC EXTRACT OF SELECTED SEED SHELLS

2020 ◽  
pp. 74-76
Author(s):  
MOUSHUMI BAIDYA ◽  
ANBU J. ◽  
SEMIMUL AKHTAR ◽  
SIPRA SARKAR ◽  
SUDIP KUMAR MANDAL
Keyword(s):  
Antimicrobial Activity ◽  
Fungal Pathogens ◽  
Antimicrobial Properties ◽  
Ethanolic Extract ◽  
Diffusion Method ◽  
Local Market ◽  
Bacterial Strains ◽  
Study Results ◽  
Fungal Organisms ◽  
Antimicrobial Evaluation

Objective: The study was undertaken to evaluate the antimicrobial activity of ethanolic extract of polyherbal seed shells. Methods: The seed of Momordica charantia, Manikara zapota, Emblica officinalis, Syzygium cumini, collected from the local market, Mathikere, Bangalore, India. Ethanolic extract was prepared from the dried seed powders using solvent 80% ethanol. Initially, antimicrobial activity of the extract was performed by agar well diffusion method against two bacterial strains (Escherichia coli, and Staphylococcus aureus) and two fungal pathogens (Aspergillus niger and Candida albicans). Results: The antimicrobial study results revealed that the test extract was strongly inhibited the growth of bacteria, whereas it was not inhibited the growth of fungal organisms used in this study. Conclusion: The results suggest that ethanolic extract of seeds possess antimicrobial properties which can be used for the treatment of infectious diseases.

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