Single-dose acute toxicity of intraperitoneally administered Vital-Shoot containing reduced glutathione in ICR mice

2017 ◽  
Vol 41 (3) ◽  
pp. 133-136
Author(s):  
Chun-Nam Cha ◽  
◽  
Eun-Kee Park ◽  
Chang-Yeul Yoo ◽  
Suk Kim ◽  
...  
Keyword(s):  
Single Dose ◽  
Acute Toxicity ◽  
Reduced Glutathione ◽  
Icr Mice

Liver preneoplastic changes in mice treated with the herbicide fomesafen

1999 ◽  
Vol 18 (5) ◽  
pp. 338-344 ◽  
Author(s):  
J Krijt ◽  
P Stránská ◽  
J Sanitraák ◽  
A Chlumská ◽  
F Fakan
Keyword(s):  
Single Dose ◽  
Single Injection ◽  
Diphenyl Ether ◽  
Liver Histology ◽  
Mouse Strains ◽  
Toxicological Evaluation ◽  
Liver Nodules ◽  
Icr Mice ◽  
Mice Liver ◽  
Foci Of Altered Hepatocytes

1 Effect of the diphenyl ether herbicide fomesafen on liver preneoplastic changes and porphyrin biosynthesis was examined in male C57BL/6J mice (0.23% in the diet for 14 months) and ICR mice (0.3% in the diet for 50 weeks). Fomesafen treatment resulted in preneoplastic changes (liver nodules and foci of altered hepatocytes) in both strains, uroporphyria developed only in ICR mice. 2 Iron pretreatment (600 mg/kg as a single dose) accelerated the development of fomesafen-induced preneoplastic changes in both mouse strains. The number of foci containing altered hepatocytes, as well as the number and size of liver nodules, were increased in iron-pretreated animals. 3 A single injection of iron induced marked uroporphyria in C57BL/6J mice after 14 months (liver porphyrin content 102 nmol/g). This uroporphyria was further potentiated by fomesafen administration (208 nmol/g). 4 In ICR mice, liver histology was apparently normal after a 3 month recovery from fomesafen treatment (0.32% for 9 months). Liver porphyrin content (260 nmol/g) started to decrease immediately after fomesafen withdrawal, but was still significantly elevated after 3 months (5 nmol/g), as compared to controls (1 nmol/g). 5 It is concluded that the toxicological evaluation of fomesafen should focus on liver porphyrin biosynthesis.

scholarly journals Single-Dose Toxicity Study on ML171, a Selective NOX1 Inhibitor, in Mice

2021 ◽  
Vol 2021 ◽  
pp. 1-8
Author(s):  
Se-Hyun Oh ◽  
Ji-Sun Ahn ◽  
Eun-Joo Oh ◽  
You-Jin Kim ◽  
Ju-Min Yook ◽  
...  
Keyword(s):  
Single Dose ◽  
Nicotinamide Adenine Dinucleotide Phosphate ◽  
Anterior Lobe ◽  
Negative Control ◽  
Icr Mice ◽  
Injection Dose ◽  
Good Laboratory ◽  
Males And Females ◽  
Isoform Selectivity ◽  
Safe Dose

Background. ML171 is a potent nicotinamide adenine dinucleotide phosphate oxidase (NOX) inhibitor with isoform selectivity only for NOX1. This study is aimed at investigating the safety of ML171 after a single intraperitoneal (IP) injection in mice. Methods. The toxicity of a single dose of ML171 was evaluated in 6-week-old Institute of Cancer Research (ICR) mice in a good laboratory practice (GLP) laboratory. Twenty-five mice of each sex were assigned to five groups: negative control, vehicle control, and 125, 250, and 500 mg/kg of ML171. All mice were acclimatized for one week before beginning the study. Mice received an IP injection of ML171 or vehicle. The general condition and mortality of the animals were observed. The mice were sacrificed to evaluate histopathology 14 days after the administration of ML171 or vehicle. Results. Bodyweights were not significantly different in any group. Three males and one female died due to ML171 administration in the 500 mg/kg dose group. Autopsies of the surviving mice did not reveal any significant abnormalities after the injection of 125 mg/kg of ML171. However, the anterior lobe edge of the liver was thickened and adhesions between the liver and adjacent organs were observed in mice treated with 250 or 500 mg/kg of ML171. In addition, hypertrophy of centrilobular hepatocytes and inflammatory cell infiltration were observed after injection of 250 and 500 mg/kg of ML171. Conclusion. Our results indicate that the lethal IP injection dose of ML171 is 500 mg/kg for both males and females. Mortality were not observed for lower doses of ML171. The safe dose of single IP ML171 in ICR mice was 250 mg/kg or less. Further studies are needed to confirm the safety of ML171 in the human body.

scholarly journals A Study on the Acute Toxicity of Leejung-tang(Lizhong-tang) and Fermented Leejung-tang(Lizhong-tang) Extract in ICR Mice

2012 ◽  
Vol 27 (3) ◽  
pp. 95-100 ◽  
Author(s):  
Young-Pil Jung ◽  
Yun-Hwan Hwang ◽  
Ji-Hye Lee ◽  
Nam-Hui Yim ◽  
Won-Kyung Cho ◽  
...  
Keyword(s):  
Acute Toxicity ◽  
Icr Mice

scholarly journals Acute and Subacute Toxicity Profiles of the Methanol Extract of Lycopersicon esculentum L. Leaves (Tomato), a Botanical with Promising In Vitro Anticancer Potential

2020 ◽  
Vol 2020 ◽  
pp. 1-10
Author(s):  
Gaëlle S. Nguenang ◽  
Arsène S. M. Ntyam ◽  
Victor Kuete
Keyword(s):  
Single Dose ◽  
Acute Toxicity ◽  
Lycopersicon Esculentum ◽  
Methanolic Extract ◽  
Female Rats ◽  
Male Rats ◽  
Total Serum ◽  
Subacute Toxicity ◽  
Tomato Extract ◽  
Long Term Treatment

Lycopersicon esculentum (tomato) is a plant widely used in Africa like food and to solve many health problems. The methanol crude extract of tomato recently demonstrated a good antiproliferative effect on many human cancer cell lines. The aim of this research was to evaluate the acute toxicity and subacute oral toxicity of methanolic extract from leaves of this plant. These toxicities were evaluated based on the OECD (Organization for Economic Cooperation and Development) guidelines. The assay of acute toxicity was performed using a total of 3 female rats, which received a single dose of 5000 mg/kg of methanolic extract via oral gavage. For the subacute toxicity study, 32 Wistar rats (males and females) were used. The groups were treated with three different doses of Lycopersicon esculentum methanolic extract (250, 500, and 1000 mg/kg b.w.) for 28 days and the control group received distilled water. The hematological, biochemical, and histopathological studies were performed after the sacrifice. Single dose of tomato extract caused no toxicity up to a dose of 5000 mg/kg body weight; hence, the median lethal dose (DL50) of leaves of this plant was greater than this value. However, lower toxic effects could be manifested in the long-term treatment at the highest dose (1000 mg/kg) because urea level and total serum proteins significantly increased at a dose of 1000 mg/kg with respect to control. The microscopic observation showed no remarkable pathological changes on all organs in the treated groups compared with the control groups of female and male rats. These results demonstrate that single dose of tomato extract leaves is relatively nontoxic at a dose of 5000 mg/kg b.w. and prolonged use of lower doses (250 and 500 mg/kg) of L. esculentum orally should be encouraged, whereas highest dose (1000 mg/kg) should be avoided.

scholarly journals Single-dose acute toxicity of THJ-2201 designer Cannabis drug: LD50 and hematological and histological changes in mice

2018 ◽  
Vol 8 (1) ◽  
Author(s):  
Abdulsallam Bakdash ◽  
Ahmad Mohamed Khir AL-Mathloum ◽  
Elkhatim H. Abdelgadir ElAmin ◽  
Nael M. T. Abu Taha ◽  
Sachil Kumar ◽  
...  
Keyword(s):  
Single Dose ◽  
Acute Toxicity ◽  
Histological Changes

scholarly journals Safety evaluation of Aloe vera soft capsule in acute, subacute toxicity and genotoxicity study

PLoS ONE ◽  
2021 ◽  
Vol 16 (3) ◽  
pp. e0249356
Author(s):  
Jun Wu ◽  
Ying Zhang ◽  
Zhongming Lv ◽  
Ping Yu ◽  
Weiqing Shi
Keyword(s):  
Acute Toxicity ◽  
Toxicity Test ◽  
Mutagenic Activity ◽  
Aloe Vera ◽  
Safety Evaluation ◽  
Health Food ◽  
Male And Female ◽  
Subacute Toxicity ◽  
Icr Mice ◽  
Toxicological Profile

Aloe vera has been widely used in health and nutritional supplements in Chinese herbal medicine. Furthermore, Aloe vera production has been an emerging industry for making cosmetics and functional food. However, the reported adverse effects raised questions as to whether Aloe vera and its products were safe enough to be used in medicine and health care. In view of this, the safety evaluation of Aloe vera products before marketing is very important. The present study aimed to assess the toxicological profile of Aloe vera soft capsule (ASC), through acute, subacute toxicity and genotoxicity tests. Male and female ICR mice were received by oral gavage 15000 mg/kg bodyweight of ASC in the acute toxicity test. Male and female SD rats were fed on diet blended with different doses of ASC (equivalent to 832.5, 1665 and 3330 mg/kg bodyweight of ASC) for the subacute toxicity test. In the acute toxicity study, no mortality or behavioral changes were observed, indicating the LD50 was higher than 15000 mg/kg bodyweight. In the subacute toxicity test, no significant changes were observed in bodyweight, food consumption, hematological, biochemical or histopathological parameters in the rats exposed. These data suggested that ASC used in this study did not produce any marked subacute toxic effects up to a maximum concentration of 3330 mg/kg bodyweight. In the genotoxicity study, ASC showed no mutagenic activity in the Ames test and no evidence of potential to induce bone marrow micronucleus or testicular chromosome aberrations in ICR mice exposed to 10000 mg/kg bodyweight. Collectively, ASC could be considered safe before it was marketed as a laxative and moistening health food.

scholarly journals Sub-acute toxicity study in female ICR mice following repetitive intramuscular injection of cervical cancer vaccines

2014 ◽  
Vol 29 ◽  
pp. e2014024
Author(s):  
Seol-Hee Moon ◽  
Du-Yeol Kim ◽  
Jung-Min Lee ◽  
Hee-Won Park ◽  
Hye-Yeong Lee ◽  
...  
Keyword(s):  
Cervical Cancer ◽  
Acute Toxicity ◽  
Cancer Vaccines ◽  
Intramuscular Injection ◽  
Toxicity Study ◽  
Icr Mice ◽  
Acute Toxicity Study

scholarly journals Cardioprotective Effect of Marine Astaxanthin on Doxorubicin-Induced Cardiotoxicity in Normal Rats

2019 ◽  
pp. 1-11
Author(s):  
Ali A. AlQahtani ◽  
Abdel-Moneim M. Osman ◽  
Zoheir A. Damanhouri ◽  
Huda M. Al-Kreathy ◽  
Hamdan S. Al-Malky ◽  
...  
Keyword(s):  
Superoxide Dismutase ◽  
Single Dose ◽  
Serum Level ◽  
Glutathione Peroxidase ◽  
Normal Saline ◽  
Total Antioxidant Capacity ◽  
Reduced Glutathione ◽  
Cardioprotective Effect ◽  
Cardiac Enzymes ◽  
Total Antioxidant

Background: Doxorubicin (DOX) is an effective antineoplastic drug indicated to treat many cancerous diseases but its clinical usefulness is limited by many side effects. The main and the most serious one is DOX induced cardiotoxicity. Many strategies have been tried to minimize this side effect such as addition of cardioprotective agent to DOX treatment protocols. Aims: The aim of this work was directed to investigate whether marine astaxanthin (ATX), a xanthophyll carotenoid pigment with potent antioxidant effect, could protect heart against the cardiotoxicity induced by DOX. Methodology: Forty Male Wister rats were divided into four equal groups and treated for one week as follow: Group I rats were treated with normal saline (2 ml/kg, x7, i.p.) and considered a control group. Group II rats were treated with ATX (40 mg/kg, x7, i.p.). Group III rats were treated with normal saline (2 ml/kg, x7, i.p.) and a single dose of DOX (20 mg/kg, i.p.) at day 7. Finally, group IV rats were treated with ATX (40 mg/kg, x7, i.p) and with a single dose of DOX (20 mg/kg, i.p.) at day 7. After 24 and 48 hrs of treatment, rats were anesthetized and prepared for collection of blood samples and heart isolation. The cardioprotective effect of ATX against DOX induced cardiotoxicity were evaluated by measurement of the serum level of cardiac enzymes CPK by colorimetric assay and CK-MB by Eliza. Also the levels of serum total antioxidant capacity (TAC) were measured colorimetrically. In addition, the Malondialdehyde (MDA), reduced glutathione, glutathione peroxidase (GPx) levels and superoxide dismutase (SOD) were determined in heart tissues homogenate by colorimetric method. In addition, Heart sample were taken for histopathology studies. Results: The Addition of ATX to DOX significantly (p<0.05) decreased the serum level of cardiac enzymes (CPK, CK-MB) and increased the serum total antioxidant capacity in compare with these levels in sera of rats treated with DOX only. This addition also significantly decreased the level of malondialdehyde and increased the reduced glutathione and glutathione peroxidase and superoxide dismutase significantly in the heart tissues homogenate in compare to corresponding levels in rats treated with DOX alone. Histopathological investigation of cardiac tissues confirmed the biochemical studies, where addition of ATX to DOX treatment protocol showed that the fragmentation of the muscle fiber revealed normal with central vesicular nuclei and prevented a marked disruption of normal cardiac architecture which resulted from DOX treatment. Conclusion: Marine astaxanthin provides excellent cardioprotective effect against doxorubicin induced cardiotoxicity in rats.

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