scholarly journals Alpine Pioneer Plants in Soil Bioengineering for Slope Stabilization and Restoration: Results of a Preliminary Analysis of Seed Germination and Future Perspectives

2020 ◽  
Vol 12 (17) ◽  
pp. 7190
Author(s):  
Luca Giupponi ◽  
Valeria Leoni
Keyword(s):  
Calcium Carbonate ◽  
Seed Germination ◽  
Room Temperature ◽  
Restoration Ecology ◽  
Germination Rate ◽  
Ex Situ ◽  
Pioneer Species ◽  
Soil Bioengineering ◽  
Pioneer Plants ◽  
Dryas Octopetala

Stabilization of slopes subject to landslide by measures with low impact, such as those of bioengineering, is a topic of interest. The use of scarcely studied alpine pioneer plants could contribute to innovation in soil bioengineering and restoration ecology but to use them, knowledge of the ex situ germinability of their seeds is fundamental. This research analysed the germinability of seeds of nine alpine pioneer species (Papaver aurantiacum, Rumex scutatus, Tofieldia calyculata, Pulsatilla alpina, Silene glareosa, Adenostyles alpina, Dryas octopetala, Laserpitium peucedanoides and Laserpitium krapfii) treated with water, gibberellic acid (GA3) and/or calcium carbonate at room temperature. The seeds had different responses to the treatments: Laserpitium peucedanoides, L. krapfii and Silene glareosa showed difficulty in germinating (germination < 2.5%), while Dryas octopetala had good germination (39–61%) regardless of treatment. GA3 significantly increased the seed germination rate of Papaver aurantiacum, Pulsatilla alpina, Rumex scutatus and Tofieldia calyculata, while the addition of calcium carbonate made the seeds of Rumex scutatus and Tofieldia calyculata germinate more quickly. Results are discussed focusing on the perspectives of using alpine pioneer species in future soil bioengineering work for slopes stabilization and restoration, and on the actions that stakeholders should take to make this happen.

scholarly journals Study of the Cryopreservation Conditions for seed Materials of Chartolepis intermedia Boiss.

2021 ◽  
Vol 38 ◽  
pp. 00046
Author(s):  
Margarita Ishmuratova ◽  
Saltanat Tleukenova ◽  
Alibek Ramasanov ◽  
Elena Gavrilkova ◽  
Dmitrii Ageev
Keyword(s):  
Seed Germination ◽  
Liquid Nitrogen ◽  
Room Temperature ◽  
Germination Rate ◽  
Plastic Container ◽  
In The Wild ◽  
First Time ◽  
Karaganda Region

For the first time, the depending of germination rate and energy of germination of Chartolepis intermedia seeds, collected in the wild of Karaganda region, from morphology of seeds and conditions of cryopreservation is investigated. The maximum results for viability are fixed for dark-colored average or large seeds. The best results are determined for variant of cryopreservation in plastic container with future defrosting at the room temperature. For the increasing parameters of seed germination we recommended to freeze Chartolepis intermedia seeds with using cryoprotector sucrose in concentration 20%. As the results of the study, we developed algorithm of cryopreservation of Chartolepis intermedia seeds in liquid nitrogen.

scholarly journals FEATURES SEED REPRODUCTION OF HEDYSARUM GRANDIFLORUM PALL. EX SITU AND IN VITRO

2020 ◽  
pp. 286-293
Author(s):  
N. A. Suprun ◽  
◽  
E. V. Malaeva ◽  
S. A. Shumikhin ◽  
◽  
...  
Keyword(s):  
Seed Germination ◽  
Germination Rate ◽  
Natural Populations ◽  
Seed Vigor ◽  
Ex Situ ◽  
Optimal Temperature ◽  
High Productivity ◽  
Seed Reproduction ◽  
Optimal Mode

Hedysarum grandiflorum samples were collected from 7 natural populations of the Volgograd, Samara and Lugansk People's Republic and the features of seed reproduction were studied under ex situ and in vitro conditions. The optimal temperature for seed germination and the dependence of germination and seed vigor on the storage were revealed. We observed the maximum germination rate in the scarified seeds of H. grandiflorum of 2018 collection and it was equal to 70%.It was found that the seed germina-tion and seed vigor are low and quickly decrease with years of storage, while their scarification allows to increase these indicators. It is noted that the relatively low germination of the studied seeds is leveled by the rather high productivity of species in general. The optimal mode of sterilization of H. grandiflorum seeds was experimentally determined - 10% Lysofornin® 3000 solution for 5 minutes. The percentage of sterile seedlings was 60%.

scholarly journals Morphological characters, flowering and seed germination of the Indonesian medicinal plant Orthosiphon aristatus

2019 ◽  
Vol 20 (2) ◽  
pp. 328-337 ◽  
Author(s):  
SHALATI FEBJISLAMI ◽  
ANI KURNIAWATI ◽  
MAYA MELATI ◽  
YUDIWANTI WAHYU
Keyword(s):  
Seed Germination ◽  
Medicinal Plant ◽  
Southeast Asia ◽  
Morphological Variation ◽  
Germination Rate ◽  
Leaf Shape ◽  
Morphological Characters ◽  
Ex Situ ◽  
New Information ◽  
Ex Situ Collections

Febjislami S, Kurniawati A, Melati M, Wahyu Y. 2019. Morphological characters, flowering and seed germination of the Indonesian medicinal plant Orthosiphon aristatus. Biodiversitas 20: 328-337. Orthosiphon aristatus (Blume) Miq is a popular medicinal plant in Southeast Asia. The morphological variation of O. aristatus is narrow and information on flowering and seed germination is limited. This study aimed to determine the morphological characters, flowering and seed germination of O. aristatus. The study was conducted on 19 accessions (ex situ collections) of O. aristatus from West, Central and East Java. It was found that the differences in morphological and flowering characters were mainly based on shape and color. The dominant stem color is strong yellowish green mixed with deep purplish pink in different proportions. The dominant leaf shape was medium elliptic. O. aristatus flower has three kinds of colors: purple, intermediate and white (the most common color). O. aristatus has heterostyled flower with a long-styled morph. The stigma has two shapes: open and close (the dominant shape). The open and close stigma was found in Tuban accession, the open stigma was found in Pamekasan accession. O. aristatus accessions have a narrow diversity of 84% similarity rate. O. aristatus started flowering at 4-5 weeks after planting and blooming 2-3 weeks later. O. aristatus seeds begin to germinate on the fourth day and take about eight days to grow from seedling to become complete sprouts. Count I ranged from day 5-6 and count II on days 10-11. Tuban accession has the highest germination rate of 72.97%. The results of this research can be considered as new information, regarding information about the diversity of morphological, flowering characters and the ability of O. aristatus seed to germinate.

scholarly journals Assessment of the effect of long-term seed storage on the viability of Matricaria chamomilla seeds after cryopreservation

2021 ◽  
Vol 103 (3) ◽  
pp. 95-101
Author(s):  
Alibek Ramasanov ◽  
◽  
Larissa Babeshina ◽  
Keyword(s):  
Seed Germination ◽  
Liquid Nitrogen ◽  
Room Temperature ◽  
Germination Rate ◽  
Seed Storage ◽  
Water Bath ◽  
Moscow Region ◽  
Matricaria Chamomilla ◽  
Test Species ◽  
Viable Seeds

The article presents the study of influences of cryopreservation on seed germination of Matricaria chamomilla. Four varieties «Moscow Region», «Karagandinskaya», «Aibolit» and «Old Lekar» are used in the work. The seeds of the test species are cryopreserved in liquid nitrogen, followed by defrosting at room temperature and rapid defrosting in a water bath at temperature +40 Ԩ. After cryopreservation the seeds are planted in Petri dishes and placed in a climate chamber for determination laboratory germination. It is noted that the best results are obtained in the defrosting variant at the room temperature. So the maximum germination rate for variety «Karagandinskaya» was 84.0 %, energy of germination — 83.0 %; for «Aibolit» variety was 82.0 % and 81.0 %, respectively; «Moscow Region» variety had a minimum germination rate — 13.0 % and energy of germination 10.0 %. While for the «Old Lekar» variety, the optimal thawing mode was rapid thawing in a water bath at the temperature +40Ԩ; seed germination was 63.0 %, energy of germination — 62.0 %. Thus, it is necessary for receiving more viable seeds of varieties of Matricaria chamomilla after freezing in liquid nitrogen to apply the slow mode of thawing at the room temperature +24 Ԩ.

scholarly journals Investigation of vegetative properties and generative production of the potential ornamental and narrow endemic species Verbascum yurtkuranianum (Scrophulariaceae) for ex situ conservation

BioResources ◽  
2021 ◽  
Vol 16 (4) ◽  
pp. 7530-7549
Author(s):  
Kamil Erken
Keyword(s):  
Seed Germination ◽  
Endemic Species ◽  
Ex Situ Conservation ◽  
Germination Rate ◽  
Ultrasonic Waves ◽  
Ornamental Plant ◽  
Ex Situ ◽  
Narrow Endemic ◽  
Single Location ◽  
Number Of Individuals

Verbascum yurtkuranianum is a narrow endemic species occurring in a single location, the northern Bursa province (Turkey). It is an endangered and potentially ornamental plant. No conducted study on its life and biology, production, and aesthetic features is available. This study aimed to reveal its vegetative properties, seed characteristics, methods and requirements for seed germination, germination speed, and potential ornamental value so it can be conserved ex situ and produced. Verbascum yurtkuranianum has potential value as an ornamental plant regarding its aesthetic features as a flower. This study revealed that the total number of individuals in the species is 788. Without any treatment, 70.7% germination rate is achieved if the seeds are stored at 4 °C. The optimum germination temperature was from 15 to 20 °C (77.3% and 78.7%, respectively), and the photoperiod regulation for seed germination was 12/12 or 8/16 (light/dark) hours (74.7% and 76.0%, respectively). The most effective treatment to promote germination rate was found by implementation of 60 min ultrasonic waves (94.3%) or application of 120 min vacuum (95.3%). Germination occurred between 8 and 10 days. A parcel of ex situ conservation was constituted with the seedlings obtained from the germination studies.

scholarly journals Vegetation regeneration on natural terrain landslides in Hong Kong: direct seeding of native species as a restoration tool

2022 ◽  
Author(s):  
Ying Ki Law ◽  
Calvin Lee ◽  
Chun Chiu Pang ◽  
Billy Hau ◽  
Jin Wu
Keyword(s):  
Hong Kong ◽  
Seed Germination ◽  
Native Species ◽  
Forest Restoration ◽  
Germination Rate ◽  
Direct Seeding ◽  
Species Selection ◽  
Seed Coating ◽  
Pioneer Species ◽  
Coating Formulation

Landslides are common in tropical and subtropical regions with hilly terrains and heavy rainstorms, which cause significant economic, ecological, and social impacts. Natural forest succession is usually slow on landslide scars due to poor soil structure and the lack of seeds of woody plant seeds, and often comes with a higher risk of repeated landslide. Ecological forest restoration has recently been suggested as an effective alternative to restore the exposed landslide scars, however, a comprehensive study to identify effective landslide restoration strategies remains lacking, particularly associated with seed treatment methods and species selection. Here we evaluated the effectiveness of different seed coating treatments of both pioneer and later successional tree species of different seed sizes on seed germination in a one-year study on three landslides in Hong Kong. Our results show that bare seeds had germination rates of 17 to 67% across all selected species (n=7). Biochar-dominant seed coating formulation boosted an additional 9.33 (SE= 0.04) in seed germination rate, while the clay-dominant seed coating formulation did not show significant effect on germination. Our results also show that medium and large-seeded non-pioneer species have significantly higher germination rates than pioneer species. These results collectively suggest that direct seeding using a biochar seed coat is a manageable and useful method to enhance tree seed germination—an essential first step to restore the forests after landslide disturbances in Hong Kong, with potential to be extended to other humid tropical and subtropical forests.

scholarly journals Water immersion and one-year storage influence seed germination of Copernicia alba palm tree from a neotropical wetland

2021 ◽  
Author(s):  
Vanessa Couto Soares ◽  
L. Felipe Daibes ◽  
Geraldo A. Damasceno-Junior ◽  
Liana Baptista De Lima
Keyword(s):  
Seed Germination ◽  
Room Temperature ◽  
Ex Situ Conservation ◽  
Water Immersion ◽  
Hot Water ◽  
Ex Situ ◽  
Palm Tree ◽  
Laboratory Conditions ◽  
One Year ◽  
Water Treatments

Abstract Copernicia alba (Morong ex Morong & Britton) is a palm tree native to tropical and subtropical climates, commonly named “carandá”, that often forms monodominant populations in the Pantanal wetland in Brazil. Currently, the knowledge of the seed germination behavior of C. alba is still limited, as well as seed viability in ex-situ conservation under laboratory conditions. Hence, this study investigated the effect of water treatments and a one-year storage period (at 19°C under a relative humidity of 45%) on its germination. We subjected fresh and stored seeds to immersion in water at room temperature (~25°C) for 24, 48, and 72 h; and hot water (~75°C) for 5 min and 10 min. The results showed that seeds do not have primary dormancy, with fresh seeds germinating 84% in the control. Additionally, fresh seeds had a significant increase in germination percentage (reaching 100%) and faster germination times after immersion in water for 48 and 72 h. On the other hand, hot water treatments decreased germination for both fresh and stored seeds. One year of storage reduced the germination capacity by almost 50%. We concluded that seeds of C. alba may be easily germinated under laboratory conditions for seedling production, and water immersion at room temperature can improve the germination of fresh seeds. However, long-term seed storage and hot-water treatments may jeopardize germination. Future studies should further investigate ex-situ conservation of seed of C. alba under extended periods, considering seed desiccation and alternative storage conditions, to improve seed conservation techniques .

scholarly journals Germination and Protocorm Formation of Ophrys Sphegodes Mill. – In Vitro Protocol for a Rare Orchid Species

2018 ◽  
Vol 67 (3-4) ◽  
pp. 196-201
Author(s):  
Jovana Dulić ◽  
Mirjana Ljubojević ◽  
Ines Prlainović ◽  
Goran Barać ◽  
Tijana Narandžić ◽  
...  
Keyword(s):  
Seed Germination ◽  
Germination Rate ◽  
Casein Hydrolysate ◽  
Habitat Destruction ◽  
Ex Situ ◽  
Organic Additives ◽  
Orchid Species ◽  
Short Period ◽  
Protocorm Formation

Summary Ophrys sphegodes Mill. is a wild orchid species which is threatened and protected due to its pollination biology, small seed and habitat destruction. The aim of this study was to establish asymbiotic germination protocol for the purpose of ex situ conservation. Two basal media Knudson C (KC) and Malmgren (MM), supplemented with organic additives (peptone (PE), L-glutamin (A)e, folic acid, casein hydrolysate (CA)) added separately and control media KC--C and MM--C were used in the present research. All the nutrition media contained 2% sucrose, 7% agar and 1% activated carbon, while their pH was adjusted to 5.8 ± 0.02 before autoclaving at 121 ° C for 20 minutes. The seeds were examined under two illumination conditions, 0/24 light/dark (L/D) and 16/8 L/D. The presented results indicate a huge influence of illumination and nutrition media on the seed germination and protocorm formation. The seed germination was overall significantly more successful in dark conditions (0/24 L/D) than with lighting (16/8 L/D). Protocorm, rhizoids and shoot formation were achieved only on the seeds cultured on MM medium, while the KC medium caused only swelling of the embryo. Organic additives had positive influence on the germination rate. According to the obtained results, the best germination rate and seedling development were achieved on MM-PE media, cultured in dark. The presented procedure accelerates the germination period and can provide a large number of plants in a relatively short period of time so it can be used for conservation programs and mass production protocol.

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