scholarly journals Molecular and morphological characterization of EMS-induced chili pepper mutants resistant to Chili veinal mottle virus

2020 ◽  
Vol 21 (4) ◽  
Author(s):  
Ifa Manzila ◽  
Tri Puji Priyatno ◽  
KRISTIANTO NUGROHO ◽  
RERENSTRADIKA TIZAR TERRYANA ◽  
Puji Lestari ◽  
...  

Abstract. Manzila I, Priyatno TP, Nugroho K, Terryana RT, Lestari P, Hidayat SH. 2020. Molecular and morphological characterization of EMS-induced chili pepper mutants resistant to Chili veinal mottle virus. Biodiversitas 21: 1448-1457. Chili veinal mottle virus (ChiVMV) is a major prevalent virus that potentially reduces the yield of chili pepper by more than 50% in Indonesia. Therefore, the development of a ChiVMV-resistant cultivar is necessary to ensure satisfactory chili pepper production. Mutation breeding is a promising tool available to produce virus-resistant plants via the induction of new alleles due to point mutations within the existing chili germplasm. This research was conducted to assess the genetic variation of ten promising (M6) EMS-induced mutant lines of chili pepper (Capsicum annuum L.) potentially resistant to ChiVMV by their morphological characteristics and SSR profile, as well as identify potential mutants based on agronomic traits. The ten chili pepper mutant lines used in the present study were derived from the shoot tips of a Gelora cultivar treated with 0.5% ethyl methanesulfonate (EMS) as a chemical mutagen. The performance of the respective promising chili pepper mutant lines both at a molecular and morphological level was observed. Phenotypic analysis showed that all the promising chili pepper mutant lines underwent significant morphological changes. Of these, the phenotype coefficient variation (PCV) values were higher than the genotype coefficient variation (GCV) values for all the traits observed. High heritability (h2) was found for the canopy area, length of the fruit stalk, fruit weight, fruit weight per plant, thick fruit flesh, leaf width, leaf length, and fruit storage duration. A total of 52 alleles were detected from the five polymorphic SSR loci with 5.2 average alleles/loci. A dendrogram was generated and revealed two groups with five mutants clustered separately from their parents which could be valuable for further improvement.

2021 ◽  
Author(s):  
Diego Cunha Zied ◽  
Wagner G. Vieira Junior ◽  
Douglas M. M. Soares ◽  
Cassius V. Stevani ◽  
Eustáquio S. Dias ◽  
...  

Abstract The mushroom Agaricus subrufescens has been synonymous with Agaricus blazei and Agaricus brasiliensis during the last decades and there has been much discussion with regards to the origin, distribution, and nomenclature of this mushroom. Therefore, we conducted a genetic and morphological characterization of the mycelium and mushroom of four commercial strains currently cultivated in Brazil (ABL CS7, ABL 18/01, ABL 98/11, and ABL 16/01) together with an assessment of their agronomic behavior and compared these results with those of other strains used during the last 15 years. All the A. subrufescens strains characterized here are phylogenetically related to the Americas/Europe specimens, bearing an internal transcribed spacer region of type A (ABL 16/01) or both types A and B (ABL 18/01, ABL 98/11, and ABL CS7). We did not find any correlation between the morphological characteristics of the mycelial colonies and the agronomic behavior of the strains. Strains ABL 98/11 and ABL 16/01 produced the best yields and morphological characteristics for the mushrooms, indicating their high weight, which enhances the commercialization of the mushroom and justifies their longstanding commercial use over the last 15 years.


HortScience ◽  
2018 ◽  
Vol 53 (9) ◽  
pp. 1266-1270 ◽  
Author(s):  
Nader R. Abdelsalam ◽  
Hayssam M. Ali ◽  
Mohamed Z.M. Salem ◽  
Elsayed G. Ibrahem ◽  
Mohamed S. Elshikh

Mango (Mangifera indica L.) is a fruit crops belong to the family Anacardiaceae and is the oldest cultivated tree worldwide. Cultivars maintained in Egypt have not been investigated previously. Mango was first brought to Egypt from South Asia. Morphological and molecular techniques were used to identify the genetic diversity within 28 mango cultivars. SSR and EST-SSR were used for optimizing germplasm management of mango cultivars. Significant variations were observed in morphological characteristics and genetic polymorphism, as they ranged from 0.71% to 100%. High diversity was confirmed as a pattern of morphological and genotypes data. Data from the present study may be used to calculate the mango relationship and diversity currently grown in Egypt.


2020 ◽  
Vol 73 (1) ◽  
pp. 9019-9027
Author(s):  
Jorge Andres Betancur G. ◽  
Sandra Bibiana Muriel R. ◽  
Elena Paola Gonzalez J.

The red dragon fruit Selenicereus undatus is a native fruit from the Americas. Its growing appeal in the international market as an exotic fruit highlights the relevance of the crop for the local economies. In Colombia, the high diversity within the genus and species makes it difficult to have reliable materials for propagation as cultivars. This agronomic challenge requires more studies of the distribution and real diversity within the genus Selenicereus sp. Due to the information aforementioned, the fruit is harvested in small localities as an extraction production system. This project aimed to morphologically characterize the working collection of red dragon fruit under growing conditions in the Municipality of San Jerónimo. It contained 30 accessions from the departments of Antioquia, Córdoba, and Valle del Cauca. Twenty-one characters of cladode, flower, and fruit were quantified to assess the diversity, and a restricted cluster analysis was performed to understand the morphological richness of the materials. As a result, it was observed differences in terms of stems, such as distance between areolas and width of cladode. This was also observed in flowers, in which their total length, the width of petals, the presence of trichomes, the color of calyx and petals, and the color of the stigma were different. Also, differences were recorded in the fruit. Characteristics such as weight and color of its skin were assessed, resulting in fruit size medium to small. Regarding other properties, total soluble solids varied between 11.2 to 15.6 °Brix. 55% of the fruits have measurements that fit market requirements. Finally, three accessions match the requirements of weight and °Brix, to be used as possible genotypes by commercial growers; however, studies about agronomic management strategies that favor an increase in fruit weight must be done.


Zootaxa ◽  
2020 ◽  
Vol 4821 (1) ◽  
pp. 49-70
Author(s):  
ASHOK KUMAR MEENA ◽  
RAJAMANI SWAMINATHAN ◽  
RAJENDRA NAGAR

The present paper deals with a description of two new species of crickets of the subfamily Podoscirtinae (Orthoptera: Gryllidae), Trelleora khasiana sp. nov. and Indotrella maculata sp. nov., from India; besides, some additional morphological characteristics of the already reported species, Indotrella angusta Gorochov, 2003 is also presented. The morphological characterization of these two new species is supported with suitable line diagrams and plates. A checklist of crickets of Podoscirtinae for the Asia-tropical region is included.


2014 ◽  
Vol 6 (2) ◽  
pp. 451-456 ◽  
Author(s):  
K. K. Sharma ◽  
U. S. Singh

The genus Trichoderma contains species that are of a great economic importance due to their ability to act as biological control agents against a large variety of fungal plant pathogens. In the present investigation thirty isolates of the Trichoderma sp. were obtained from the rhizosphere soils of different plants at different locations at Nainital, Almora, Udham Singh Nagar, Derhadun, Haridwar and Tehri Garhwal districts of Uttarakhand (India). The isolates were characterized on the basis of their cultural and morphological characteristics. The cultural characteristics included linear growth, colony colour, pigmentation and growth pattern. Morphological characteristics studied were structure, shape and arrangement of conidiophores, phialides and conidia. Out of thirty isolates, 6 isolates namely PB10, PB13, PB23, PB26, PB27 and PB28 were identified as T. virens and remaining 24 isolates as T. harzianum.


2019 ◽  
Vol 10 (2) ◽  
pp. 559
Author(s):  
N.P. Sakthi Avinash ◽  
K. Manonmani ◽  
R. Muthuvijayaragavan ◽  
S. Rajeswari ◽  
S. Manonmani ◽  
...  

2020 ◽  
Vol 3 (6) ◽  
pp. 48-54
Author(s):  
Lino Manuel Vicente Sangumbe ◽  
◽  
Rosalina Jamba Calucongolo ◽  
David Mande Mário ◽  
Marcelo José Garcia Villagra

This work aimed to morphologically characterize Eucalyptus dunni and E. urograndis to the application of hydrogel. By determining the morphological parameters of the seedlings and then of the plantations in the field, after the application of the hydrogel, the characterization was carried out. The first stages, was the production of seedlings - where their morphological characteristics were determined. The second was the evaluation of growth in the field - where the morphological characteristics of the plants were determined when applying the hydrogel. The results showed that the morphological characteristics of the seedlings were adequate for the final place (field). In the field, the best result were achieved in T1, thus demonstrating the efficiency of the hydrogel.


2017 ◽  
Vol 65 (2) ◽  
pp. 555-569 ◽  
Author(s):  
A. Orobiyi ◽  
L. Y. Loko ◽  
F. Sanoussi ◽  
A. P. Agré ◽  
N. Korie ◽  
...  

2021 ◽  
Vol 4 (1) ◽  
pp. 55-64
Author(s):  
Intan Sari ◽  
Henri Henri ◽  
Eka Sari ◽  
Suharyanto Suharyanto

Ginseng plant (Phytolacca octandra L.) is a wild plant that has the potential to be used as medicine. The morphological characteristics of ginseng plants in Indonesia are different. Therefore, it is necessary to carry out research aimed at characterizing the morphology and phytochemical screening of ginseng (P. octandra L.) leaves and tubers. The research was conducted first by observing morphological characters and screening phytochemical compounds such as alkaloid test, flavonoid test, phenol test, tannin test, saponin test, terpenoid test, and steroid test. The results of the research on the morphological characterization of ginseng (P. octandra L.) were: having a taproot which was modified into a tuber, a round, smooth, red color, pointed leaf shape, the base and tip of the leaf were pointed, buni fruit type, round flat, colored blackish-purple when ripe, a compound flower is located at the end of the branch and has flat, round seeds. Ginseng tubers have triterpenoid and saponin compounds, while the leaves only have saponin compounds. The potential development of ginseng (P. octandra L.) is currently limited as traditional medicine by local people. This is due to limited knowledge in its development efforts and unknown phytochemical content.


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