scholarly journals Acclimating leaf celery plant (Apium graveolens) via bottom wet culture for increasing its adaptability to tropical riparian wetland ecosystem

2020 ◽  
Vol 22 (1) ◽  
Author(s):  
Benyamin Lakitan ◽  
KARTIKA KARTIKA ◽  
SUSILAWATI SUSILAWATI ◽  
ANDI WIJAYA
Keyword(s):  
Natural Habitat ◽  
Total Yield ◽  
Apium Graveolens ◽  
Potential Candidate ◽  
Wetland Ecosystem ◽  
Estimation Model ◽  
Shallow Water Table ◽  
Riparian Wetland ◽  
Spad Value ◽  
Celery Plant

Abstract. Lakitan B, Kartika, Susilawati, Wijaya A. 2021. Acclimating leaf celery plant (Apium graveolens) via bottom wet culture to increase its adaptability to the tropical riparian wetland ecosystem. Biodiversitas 22: 320-328. Bottom-wet culture was set up for acclimating leaf celery plant prior to cultivation at shallow water table conditions. The aim of this research was to evaluate adaptability of leaf celery plants to riparian wetland ecosystem. Leaf celery was selected as potential candidate since natural habitat of its wild relatives is marshlands. Shading at 0%, 20%, and 60% was applied to reduced tropical sunlight intensity. Results of this study indicated that soil moisture was significantly increased in plants exposed to 60% shading, but leaf SPAD value was not significantly affected. Leaf celery is a perennial vegetable that can be frequently harvested. Weekly harvesting was rewarded with optimum yield and good quality leaves, i.e. high SPAD value (45.73 to 51.89). Delaying harvest to 3 weeks increased total yield but 52.12% of the harvested leaves were non-marketable. Mother plant of leaf celery produced suckers, but number of suckers only moderately correlated with yield (R2 = 0.56). Plants exposed to 60% shading produced significantly less suckers (9.00) than those exposed to full sunlight (12.46) and 20% shading (12.88) Use of zero intercept linear regression model, with length of leaf midrib (LLM) x leaf wingspan (LWS) as predictor, resulted in a geometrically based and accurate leaf area estimation model (LA = 0.3431(LLM x LWS); R2 = 0.87) for compound leaves of leaf celery plant. In conclusion, the most crucial factor in optimizing quantity and quality of yield was weekly harvesting focusing on marketable-size leaves.

scholarly journals Antioxidant Activities of Extracts from Celery Leaves (Apium Graveolens L) Grown in Jos, Nigeria

2020 ◽  
pp. 1-5
Author(s):  
M. Suleman Stephen ◽  
E. A. Adelakun ◽  
J. H. Kanus ◽  
Meshack M. Gideon
Keyword(s):  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Antioxidant Activities ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Ethanolic Extract ◽  
Acetone Extract ◽  
Apium Graveolens ◽  
Isolation And Identification ◽  
Celery Plant

The presence of natural antioxidant in plants is well known. Plant phenolics constitute one of the major groups of components that act as antioxidant and free radical terminator. Hence, this study focused on investigating the antioxidant activity of Celery plant (Apium graveolens L). The fresh leaves were collected, crushed and extracted with ethanol and acetone by maceration. The radical scavenging properties of the extracts were determined by measuring changes in absorbance of DPPH radical at a wave lenght of 517 nm by UV and ascorbic acid is used as the standard. It showed that the crude ethanolic extract has higher antioxidant activity compared to ascorbic acid and acetone extract with less scavenging activity. The values were (IC50 114.6 µg/mL) for ascorbic acid, (IC50 112 µg/mL) for the crude ethanolic extract and (IC50172 µg/mL) for crude acetone extract. The result shows that Celery plant grown in Jos possess good antioxidant properties which may be linked to the presence of phenolics and flavonoids in the plant, which justifies its use as a medicinal plant. This can be further investigated for the isolation and identification of active compounds of medicinal utilities.

scholarly journals Association of ‘Candidatus Liberibacter solanacearum’ with a Vegetative Disorder of Celery in Spain and Development of a Real-Time PCR Method for Its Detection

2014 ◽  
Vol 104 (8) ◽  
pp. 804-811 ◽  
Author(s):  
Gabriela R. Teresani ◽  
Edson Bertolini ◽  
Ana Alfaro-Fernández ◽  
Carmen Martínez ◽  
Francisco André Ossamu Tanaka ◽  
...  
Keyword(s):  
Real Time ◽  
Apium Graveolens ◽  
Candidatus Liberibacter Solanacearum ◽  
Test Kit ◽  
Typical Morphology ◽  
Candidatus Liberibacter ◽  
Pcr Method ◽  
Polymerase Chain ◽  
Celery Plant ◽  
Rna Genes

A new symptomatology was observed in celery (Apium graveolens) in Villena, Spain in 2008. Symptomatology included an abnormal amount of shoots per plant and curled stems. These vegetative disorders were associated with ‘Candidatus Liberibacter solanacearum’ and not with phytoplasmas. Samples from plant sap were immobilized on membranes based on the spot procedure and tested using a newly developed real-time polymerase chain reaction assay to detect ‘Ca. L. solanacearum’. Then, a test kit was developed and validated by intralaboratory assays with an accuracy of 100%. Bacterial-like cells with typical morphology of ‘Ca. Liberibacter’ were observed using electron microscopy in celery plant tissues. A fifth haplotype of ‘Ca. L. solanacearum’, named E, was identified in celery and in carrot after analyzing partial sequences of 16S and 50S ribosomal RNA genes. From our results, celery (family Apiaceae) can be listed as a new natural host of this emerging bacterium.

scholarly journals Volatile metabolites of endophytic Klebsiella aerogenes from Zingiber zerumbet rhizome and its antagonistic effect on soft rot causative Pythium myriotylum

2020 ◽  
pp. 122-132
Author(s):  
K. Harsha ◽  
R. Aswati Nair
Keyword(s):  
Ethyl Acetate ◽  
Methyl Esters ◽  
Natural Habitat ◽  
Ethanol Extract ◽  
Soft Rot ◽  
Antagonistic Effect ◽  
Absolute Methanol ◽  
Potential Candidate ◽  
Zingiber Zerumbet ◽  
Volatile Metabolites

Rhizomes of Zingiber zerumbet collected from their natural habitat and reported earlier to have high zerumbone content were selected for isolation of endophytes. Biochemical and molecular characterization using 16S rRNA sequencing of the endophytes identified the isolates as belonging to genus Klebsiella, Pantoea and Enterobacter. Isolate designated ZzKSD8 identified as K. aerogenes yielded maximal antagonistic activities against P. myriotylum determined as 83.5% ± 0.77. Volatile metabolites produced by ZzKSD8 caused absolute impairment of P. myriotylum hyphal growth compared to control (23.9 ± 0.37 cm). Volatile metabolites were extracted from 48 hour grown stationary phase ZzKSD8 cultures using absolute methanol, ethyl acetate and ethanol (60% v/v). GC-MS metabolite profiling detected alkanes and fatty acid methyl esters as the predominant constituents in the solvent extracts. Major constituents included methyl palmitate (31.37%), methyl stearate (18.57%) and cyclopropaneoctanoic acid, 2-hexyl-, methyl ester (17.05%) in methanol extract; alkanes like tetratetracontane (13.18%) and 2-methyloctacosane (12.10%) in ethyl acetate extracts and 4,22-sigmastadiene-3-one as major metabolite (22.51%) and stigmast-5-en-3-ol, (3.beta) (17.40%) in ethanol extract. Identified metabolites reported to modulate defense strategies in plants against phytopathogens makes ZzKSD8 a potential candidate for development of biological alternatives to control soil-borne soft-rot disease.

scholarly journals Field-Scale Evaluation of Botanical Extracts Effect on the Yield, Chemical Composition and Antioxidant Activity of Celeriac (Apium graveolens L. Var. rapaceum)

Molecules ◽  
2020 ◽  
Vol 25 (18) ◽  
pp. 4212
Author(s):  
Katarzyna Godlewska ◽  
Paweł Pacyga ◽  
Izabela Michalak ◽  
Anita Biesiada ◽  
Antoni Szumny ◽  
...  
Keyword(s):  
Chemical Composition ◽  
Higher Plants ◽  
Antioxidant Activities ◽  
Total Yield ◽  
Red Clover ◽  
Dry Weight ◽  
Field Trials ◽  
Apium Graveolens ◽  
Ultrasound Assisted Extraction ◽  
Botanical Extracts

The use of higher plants for the production of plant growth biostimulants is receiving increased attention among scientists, farmers, investors, consumers and regulators. The aim of the present study was to examine the possibility of converting plants commonly occurring in Europe (St. John’s wort, giant goldenrod, common dandelion, red clover, nettle, valerian) into valuable and easy to use bio-products. The biostimulating activity of botanical extracts and their effect on the chemical composition of celeriac were identified. Plant-based extracts, obtained by ultrasound-assisted extraction and mechanical homogenisation, were tested in field trials. It was found that the obtained formulations increased the total yield of leaves rosettes and roots, the dry weight of leaves rosettes and roots, the content of chlorophyll a + b and carotenoids, the greenness index of leaves, the content of vitamin C in leaves and roots. They mostly decreased the content of polyphenols and antioxidant activities in leaves but increased them in roots and conversely affected the nitrates content. Extracts showed a varied impact on the content of micro and macroelements, as well as the composition of volatile compounds and fatty acids in the celeriac biomass. Due to the modulatory properties of the tested products, they may be used successfully in sustainable horticulture.

scholarly journals Biofertilizer: Azolla pinnata in-combination with Inorganic Fertilizer on Growth and Yield of Rice

2021 ◽  
Author(s):  
Mohamad Izdzuan bin Mohamad Khair ◽  
Elisa Azura Azman ◽  
Roslan Ismail ◽  
Muhammad Naim Fadzli Abdul Rani
Keyword(s):  
Significant Influence ◽  
Soil Analysis ◽  
Block Design ◽  
Total Yield ◽  
Inorganic Fertilizer ◽  
Growth And Yield ◽  
Azolla Pinnata ◽  
Significant Treatment ◽  
Total N ◽  
Spad Value

Abstract A study was conducted at MARDI Seberang Perai, Penang. This study aims to examine Azolla pinnata on the growth and performance of the MR 297 variety. The experiment consisted of five treatments; PK + Azolla (T1); NP + Azolla (T2); NK + Azolla (T3); NPK-Control (T4), and Azolla only (T5). Each treatment has four replications. The experimental design used was a complete randomized block design (RCBD), and all data collected were analyzed using one-way ANOVA with a statistically significant 0.05% test. For the average soil analysis between the beginning and end, all soil analyses showed decreased soil properties except Total N (%) and organic carbon (%). Treatment has a significant influence on the tiller number, the number of panicles, and the yield per pot. There is a significant influence on treatment on plant height and SPAD value in crop growth performance. There was no significant effect on N and P in plant nutrients. In contrast, there was a significant treatment effect on K. This study showed that Azolla could be used as an alternative fertilizer on rice fields because the soil treated with NK + Azolla shows a comparable result with soil treated with inorganic fertilizer without Azolla on the total yield.

scholarly journals Identification and Characterization of an O-Succinyl-L-Homoserine Sulfhydrylase From Thioalkalivibrio sulfidiphilus

2021 ◽  
Vol 9 ◽  
Author(s):  
Wen-Yuan Zhu ◽  
Kun Niu ◽  
Peng Liu ◽  
Yu-Hang Fan ◽  
Zhi-Qiang Liu ◽  
...  
Keyword(s):  
Large Scale ◽  
Total Yield ◽  
Maximum Activity ◽  
Potential Candidate ◽  
Natural Amino Acid ◽  
Gene Encoding ◽  
Broad Application ◽  
Identification And Characterization ◽  
Application Prospects

L-methionine is an important natural amino acid with broad application prospects. A novel gene encoding the enzyme with the ability to catalyze O-succinyl-L-homoserine (OSH) to L-methionine was screened and characterized. The recombinant O-succinyl-L-homoserine sulfhydrylase from Thioalkalivibrio sulfidiphilus (tsOSHS) exhibited maximum activity at 35°C and pH 6.5. OSHS displayed an excellent thermostability with a half-life of 21.72 h at 30°C. Furthermore, the activity of OSHS increased 115% after Fe2+ added. L-methionine was obtained with a total yield reaching 42.63 g/L under the concentration of O-succinyl-L-homoserine 400 mM (87.6 g/L). These results indicated that OSHS is a potential candidate for applying in the large-scale bioproduction of L-methionine.

scholarly journals Effect of the Biofertilizer (Azolla pinnata) in Combination with Inorganic Fertilizers on Growth and Yield of Rice

2021 ◽  
Vol 04 (04) ◽  
pp. 59-75
Author(s):  
Mohamad Izdzuan bin Mohamad Khair ◽  
◽  
Elisa Azura Azman ◽  
Roslan Ismail ◽  
Muhammad NaimFadzli Abdul Rani ◽  
...  
Keyword(s):  
Soil Analysis ◽  
Block Design ◽  
Total Yield ◽  
Growth And Yield ◽  
Azolla Pinnata ◽  
Significant Treatment ◽  
Total N ◽  
Spad Value ◽  
Randomized Block Design ◽  
And Performance

This study was conducted at Malaysia Agriculture Research and Development Institute (MARDI), Seberang Perai, Penang (Malaysia), that aims to examine the impacts of the application of Azolla pinnata on the growth and performance of rice of the MR 297 variety. The experiment consisted of five treatments: PK + Azolla (T1); NP + Azolla (T2); NK + Azolla (T3); NPK-Control (T4), and Azolla only (T5). Each treatment had four replicates. The experimental design used was a complete randomized block design (RCBD), and all data collected were analyzed using one-way ANOVA with a statistically significant 0.05% test. For the average soil analysis between the beginning and end, all soil analyses showed decreased soil properties except total N and organic carbon. There is a significant effect on the tiller, panicles, yields, plant height, and SPAD value in crop growth performance. There was no significant effect observed on N and P among plant nutrients. In contrast, there was a significant treatment effect on K. This study concluded that the soil treated with NK + Azolla showed a comparable result with soil treated with inorganic fertilizer only for the total yield.

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