scholarly journals Morphological evaluation and determination keys of 21 citrus genotypes at seedling stage

2021 ◽  
Vol 22 (3) ◽  
Author(s):  
Rahmat Budiarto ◽  
Roedhy Poerwanto ◽  
Edi Santosa ◽  
Darda Efendi
Keyword(s):  
Cluster Analysis ◽  
Morphological Characters ◽  
Seedling Stage ◽  
Citrus Reticulata ◽  
Morphological Markers ◽  
Vegetative Shoot ◽  
Mature Tree ◽  
Complete Linkage ◽  
Morphological Evaluation ◽  
Citrus Varieties

Abstract. Budiarto R, Poerwanto R, Santosa E, Efendi D. 2021. Morphological evaluation and determination keys of 21 citrus genotypes at seedling stage. Biodiversitas 22: 1570-1579. The identification of citrus varieties is generally based on flower, fruit, and mature tree characters. The detailed and comprehensive identification of seedling stage is very limited, therefore present study aimed to identify and distinguish 21 citrus genotypes based on 50 morphological characters of vegetative shoot at seedling stage. Cluster analysis using complete linkage agglomerative method showed broader dissimilarities between C. x limon and C. x microcarpa. Unfortunately, this method was limited to differentiate six genotypes within Citrus reticulata Blanco due to extremely low dissimilarities found. All citrus seedlings have similarities in the forms of habitus, gland spots, arrangement and venation of leaf. The result of PCA determined petiole wing, spine, color, hair and fragrance of leaves as five morphological markers at seedling stage. In addition, there was a positive correlation between spine and leaf pleasant. Moreover, the details of morphological dissimilarities between genotypes were described in arranged determination keys.

scholarly journals Radiosensitivitas dan Seleksi Mutan Putatif Jeruk Keprok Garut (Citrus reticulata L.) berdasarkan Penanda Morfologi

2015 ◽  
Vol 43 (2) ◽  
pp. 126
Author(s):  
Karyanti , ◽  
Agus Purwito ◽  
Ali Husni
Keyword(s):  
Gamma Irradiation ◽  
Somatic Embryos ◽  
Lethal Dose ◽  
Morphological Characters ◽  
Callus Growth ◽  
Citrus Reticulata ◽  
Morphological Markers ◽  
Citrus Breeding ◽  
Maturation Medium ◽  
The Subject

<p>ABSTRACT</p><p>Mandarin Citrus cv. garut is a of local citrus variety with several superiority, but not competitive to imported citrus. Quality improvement have been the subject of citrus breeding programme. The objective of this research was to determine lethal dose 50 (LD50) and putative mutans regenerant selected based on morphological markers. Callus was irradiated at doses of 0, 20, 30, 40, 50, 60, 70, 80, 90 and 100 Gy and regenerated through somatic embryogenesis. The result of radiosensitivity showed that lethal dose 50 (LD50) analyzed by Curve Expert 1.4 software was 75.31 Gy. Observation on callus showed variation on morphology and weight of callus. Callus growth was not inhibited at doses 0-50 Gy but at doses 60-100 Gy callus growth was inhibited. Gamma irradiation also affected the formations of somatic embryos. After six weeks on maturation medium, the highest number of embryo somatic was produced from doses of 20 and 100 Gy and following culture on germination medium, the highest number of planlet was produced from doses of 20 and 40 Gy. Based on morphological characters, the selected regenerants had variability of 0-58%.</p><p>Keywords: gamma irradiation, germination, lethal dosis 50 (LD50), maturation, somatic embryo</p>

scholarly journals Diversity of Common Bean Landraces Collected in the Western and Eastern Carpatien

2011 ◽  
Vol 39 (No. 3) ◽  
pp. 73-83 ◽  
Author(s):  
O. Horňáková ◽  
M. Závodná ◽  
M. Žáková ◽  
J. Kraic ◽  
F. Debre
Keyword(s):  
Cluster Analysis ◽  
Common Bean ◽  
Morphological Characters ◽  
Molecular Data ◽  
Seed Traits ◽  
Phaseolus Vulgaris L ◽  
Growth Type ◽  
Banding Patterns ◽  
Polymorphism Detection ◽  
Thousand Seed Weight

The study of diversity in common bean was based on morphological and agronomical characteristics, differentiation of collected accessions by morphological and molecular markers, detection of genetic variation, and duplicates detection in bean landraces. The analysed 82 accessions of common bean (Phaseolus vulgaris L.) were collected in the Western andEastern Carpatien as landrace mixtures. Their seeds were segregated and pooled according to their characteristics; they were further multiplicated, and introduced into the collection. An extensive variation in plant and seed traits was discovered in thirty-three morphological and agronomical characteristics. Nevertheless, some of the accessions were identical in these characteristics. Cluster analysis grouped genotypes into two main branches, reflecting the growth type, seed size parameters, and thousand-seed weight. Molecular differentiation studies were performed by multilocus polymorphism detection in microsatellite and minisatellite DNA regions. Cluster analysis based on molecular data also grouped genotypes but no linkage to morphological traits was revealed. Bean accessions with very similar or identical morphological characters were clearly distinguished by DNA banding patterns. The presence of duplicates was excluded. &nbsp;

scholarly journals The Significance of Macronutrients in Alternate Bearing ‘Nadorcott’ Mandarin Trees

HortScience ◽  
2018 ◽  
Vol 53 (11) ◽  
pp. 1600-1609 ◽  
Author(s):  
Ockert P.J. Stander ◽  
Graham H. Barry ◽  
Paul J.R. Cronjé
Keyword(s):  
Treatment Effects ◽  
Foliar Spray ◽  
Shoot Development ◽  
Citrus Reticulata ◽  
Alternate Bearing ◽  
Vegetative Shoot ◽  
Current Season ◽  
Standard Practice ◽  
Main Determinants ◽  
Fruit Load

The significance of macronutrients nitrogen (N), phosphorus (P), potassium (K), calcium (Ca), and magnesium (Mg) in leaves was studied in relation with their possible roles in alternate bearing of ‘Nadorcott’ mandarin (Citrus reticulata) trees over a period of three seasons. Fruit load (“on,” a heavy fruit load, vs. “off,” a light fruit load) affected the leaf macronutrient concentrations, and the amount of macronutrients removed through the harvest of fruit, i.e., the crop removal factor (g·kg−1), was consistent in both seasons. The crop removal factors were higher for each macronutrient in “off” trees—harvest of 1 kg fruit removed ≈2.3 g·kg−1 N, 0.3 g·kg−1 P, 3.1 g·kg−1 K, 1.0 g·kg−1 Ca, and 0.4 g·kg−1 Mg, compared with 1.3 g·kg−1 N, 0.2 g·kg−1 P, 1.7 g·kg−1 K, 0.6 g·kg−1 Ca, and 0.2 g·kg−1 Mg in “on” trees. Fruit load per tree (kg/tree) of 84, 110, and 52 kg/tree in “on” trees, however, removed ≈217 g/tree N, 28 g/tree P, 296 g/tree K, 100 g/tree Ca, and 35 g/tree Mg, which was 1.5–6 times more than that of fruit loads of 14, 71, and 16 kg/tree in “off” trees. In “off” trees, N, P, and K, and in “on” trees, Ca accumulated in leaves to between 20% and 30% higher concentrations in season 1, but the higher macronutrient status did not manifest in or consistently correlate with intensity of summer vegetative shoot development in the current season, or intensity of flowering in the next season, the two main determinants of fruit load in ‘Nadorcott’ mandarin. Apart from some anomalies, the concentrations of macronutrients in leaves were unaffected by de-fruiting and foliar spray applications of N and K to “on” trees, and showed no consistent relationship with treatment effects on parameters of vegetative shoot development and flowering. Leaf macronutrients in alternate bearing ‘Nadorcott’ mandarin trees, fertilized according to grower standard practice, are not related to differences in flowering and vegetative shoot development, and appear to be a consequence of fruit load and not a determinant thereof.

scholarly journals A phenetic study of the genus Rasahus Amyot & Serville (Heteroptera, Reduviidae)

1990 ◽  
Vol 1 (3) ◽  
pp. 131-144
Author(s):  
María Coscarón
Keyword(s):  
Principal Component Analysis ◽  
Cluster Analysis ◽  
Principal Component ◽  
Component Analysis ◽  
Morphological Characters ◽  
Basal Plate ◽  
Using Data ◽  
Diagnostic Importance

Cluster analysis by four methods and a principal component analysis were performed using data on 24 morphological characters of 27 species of the genus Rasahus (Peiratinae). The results obtained by the different techniques show general agreement. They confirm the present number of taxa and reveal the existence within the genus of three groups of species: scutellaris , hamatus and vittatus. The scutellaris group is constituted by R. aeneus (Walker), R. maculipennis (Lepelletier and Serville), R. bifurcatas Champion, R. castaneus Coscarón, R. guttatipennis (Stål), R. flavovittarus Stål, R. costarricensis Coscarón, R. scutellaris (Fabricius), R. atratus Coscarón, R. peruensis Coscarón, R. paraguayensis Coscarón, R. surinamensis Coscarón, R. albomaculatus Mayr, R. brasiliensis Coscarón and R. sulcicollis (Serville).The hamatus group contains R. rufiventris (Walker), R. hamatus (Fabricius), R. amapaensis Coscarón, R. arcitenens Stål, R. limai Pinto, R. angulatus coscarón, R. thoracicus Stål, R. biguttatus (Say), R. arcuiger (Stål), R. argentinensis Coscarón and R. grandis Fallou. The vittatus group contains R. vittatus Coscarón. The characters used to separate the groups of species are: shape of the pygophore, shape of the parameres, basal plate complexity, shape of the postocular region and hemelytra pattern. Illustrations of the structures of major diagnostic importance are included.

scholarly journals Use of Highly Discriminating Morphological Characters and Isozymes in the Study of Spanish Chestnut Cultivars

2006 ◽  
Vol 131 (6) ◽  
pp. 770-779 ◽  
Author(s):  
Santiago Pereira-Lorenzo ◽  
María Belén Díaz-Hernández ◽  
Ana María Ramos-Cabrer
Keyword(s):  
Principal Component Analysis ◽  
Cluster Analysis ◽  
Multivariate Analysis ◽  
Castanea Sativa ◽  
Principal Component ◽  
Market Value ◽  
Morphological Characters ◽  
Minor Importance ◽  
Breeding Programs ◽  
And Cluster Analysis

Morphological characters (six traits) and isozymes (four systems, five loci) were used to discriminate between Spanish chestnut cultivars (Castanea sativa Mill.) from the Iberian Peninsula. A total of 701 accessions (representing 168 local cultivars) were analyzed from collections made between 1989 and 2003 in the main chestnut growing areas: 31 were from Andalucía (12 cultivars), 293 from Asturias (65 cultivars), 25 from Castilla-León (nine cultivars), four from Extremadura (two cultivars) and 348 from Galicia (80 cultivars). Data were synthesized using multivariate analysis, principal component analysis, and cluster analysis. A total of 152 Spanish cultivars were verified: 58 cultivars of major importance and 94 of minor importance, of which 18 had high intracultivar variation. Thirty-seven cultivars were clustered into 14 synonymous groups. Six of these were from Galicia, one from Castilla-León (El Bierzo), four from Asturias, one from Asturias and Castilla-León (El Bierzo), and two from Asturias, Castilla-León (El Bierzo), and Galicia. The chestnut cultivars from Galicia and Asturias were undifferentiated in genetic terms, indicating that they are not genetically isolated. Overall, chestnut cultivars from southern Spain showed the least variation. Many (58%) of Spanish cultivars produced more than 100 nuts/kg; removing this low market-value character will be a high priority. The data obtained will be of use in chestnut breeding programs in Spain and elsewhere.

Key to the species of the genus Scleria (Cyperaceae) in Costa Rica based on the morphology of achenes

Phytotaxa ◽  
2016 ◽  
Vol 284 (2) ◽  
pp. 81
Author(s):  
PATRICIA ESPINOZA ◽  
EDUARDO CHACÓN-MADRIGAL ◽  
ETHEL SÁNCHEZ ◽  
JORGE GÓMEZ-LAURITO
Keyword(s):  
Cluster Analysis ◽  
Electron Microscope ◽  
Costa Rica ◽  
Scanning Electron Microscope ◽  
Light Microscope ◽  
Digital Images ◽  
Morphological Characters ◽  
Specific Variation ◽  
Scanning Electron

We described the achenes of 21 species of the genus Scleria reported in Costa Rica using 16 morphological characters and developed a key based only on achene characteristics. Specimens deposited in herbaria in Costa Rica were analyzed. We observed the achenes using a stereoscope and light microscope and took digital images that were used to measure the achenes. Besides, the achenes were observed using a Scanning Electron Microscope. A cluster analysis using achene characteristics was performed in order to know which species are morphologically similar. The intra-specific variation of the characteristics analyzed in the achenes studied is very small for all the species. Using characteristics of the achene, we could differentiate species among four of the five traditional sections of the genus used to classify the species: Hypoporum, Ophryoscleria, Schizolepis and Scleria. The key allows differentiating among 21 species of the genus Scleria previously reported in Costa Rica using only achenes. Besides the key, we prepared an illustrative guide for the genus using pictures taken with SEM and a stereoscope. The descriptions offer better information about the species that grow in Costa Rica.

Genetic variation between and within two populations of Trifolium glomeratum (cluster clover) in Western Australia

1997 ◽  
Vol 48 (7) ◽  
pp. 969 ◽  
Author(s):  
Sarita Jane Bennett
Keyword(s):  
Cluster Analysis ◽  
Genetic Variation ◽  
Western Australia ◽  
Morphological Characters ◽  
Field Station ◽  
Site Variation ◽  
Components Analysis ◽  
And Cluster Analysis ◽  
The University ◽  
Two Populations

Genetic variation between and within populations of Trifolium glomeratum (cluster clover) was studied using seed collected from 2 sites in Western Australia: Mount Barker in the south and Kwelkan in the wheatbelt. Seed was collected at 64 subplots within each site and the material was grown at the University Field Station at Shenton Park, Perth. Seventeen morphological characters were scored and the results were analysed using analysis of variance, principal components analysis, and cluster analysis. Within-site variation was much greater than had previously been shown, and a considerable amount of between-site variation was present. It is suggested that within-site variation is due to a small amount of heterozygosity, as a result of limited outbreeding, being present in each population. The 2 populations are shown to be distinct from each other, with the population from Mount Barker containing more within-site variation. It is suggested that this is a result of climatic stress influencing and reducing the amount of variation being maintained in the Kwelkan population.

Cluster analysis of late Holocene pollen trends in Ontario

1991 ◽  
Vol 69 (8) ◽  
pp. 1719-1730 ◽  
Author(s):  
Ian D. Campbell ◽  
J. H. McAndrews
Keyword(s):  
Cluster Analysis ◽  
Geometric Mean ◽  
Ice Age ◽  
White Pine ◽  
Single Linkage ◽  
Complete Linkage ◽  
Similarity Indices ◽  
Species Abundances ◽  
Climatic Cooling ◽  
Past 1000 Years

Cluster analysis of Ontario pollen stratigraphies demonstrates similar regional successions during the past 1000 years. Seven character states qualitatively describe the behaviour of the pollen percentage trends for each taxon: 0, absent; 1, present with no visible trend but high noise; 2, rising through time; 3, falling through time; 4, rise–fall; 5, fall–rise; and 6, stable through time. The three similarity indices (S) used were of the form S equals the number of characters in agreement divided by the number of informative characters. The three clustering techniques used are single linkage, complete linkage, and unpaired weighted geometric mean analysis. Single linkage and unpaired weighted geometric mean analysis showed a north–south division with all three indices; complete linkage showed only rare local groupings with all three indices. The division between the two clusters falls just south of Lake Nipissing. All successions indicate climatic cooling; the clusters reflect southward movement of the centres of species abundances, particularly white pine. The method identifies regions of similar vegetation dynamics. Key words: cluster analysis, forest dynamics, Holocene, Little Ice Age, Ontario, palynology.

Phenetic analysis of the complex Senna fabrisii—S. trichosepala (Leguminosae, Caesalpiniodeae, Aphyllae) based on morphological characters and seed protein electrophoretic profiles

Phytotaxa ◽  
2013 ◽  
Vol 145 (1) ◽  
pp. 1 ◽  
Author(s):  
Federico O. Robbiati ◽  
Alícia Lamarque ◽  
Ana M. Anton ◽  
Renée H. Fortunato
Keyword(s):  
Cluster Analysis ◽  
Seed Protein ◽  
Principal Component ◽  
Taxonomic Status ◽  
Electrophoretic Analysis ◽  
Morphological Characters ◽  
Herbarium Specimens ◽  
And Cluster Analysis ◽  
High Degree ◽  
Univariate Analyses

Multivariate and univariate analyses based on morphological characters and seed soluble protein electrophoretic profiles were carried out in order to understand the taxonomic status within the complex S. fabrisii-S. trichosepala. Twenty morphological characters from herbarium specimens were recorded and analyzed by ANOVA, principal component and cluster analysis. The seed protein electrophoretic analysis including cluster analysis was based on three populations: one of S. fabrisii and two of S. trichosepala. The results showed that both taxa differed only by three morphological features; in parallel, the CPA and CA demonstrate that no clearly separated group can be recognized. The seed protein electrophoretic profiles show a high degree of similarity. Based on these results, and due to the variability observed, the inclusion of S. fabrisii in the synonymy of S. trichosepala is proposed.

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