Hyper-production of raw-starch-digesting enzyme by mutant fungal strain and optimisation of solid by-products

Selected fungal strain for production of raw-starch-digesting enzyme by solid state fermentation was improved by sequential exposures to gamma-irradiation of Co60, ultraviolet and treatments with N-methyl-N'-nitrosoguanidine. Mutant Aspergillus sp. CXN2-3A was chosen and its production of raw-starch-digesting enzyme (RSDE) was improved 2 folds higher than that of wild type. Optimal condition for the production of the enzyme using wheat bran as the substrate was accomplished for the CXN2-3A. With the optimal fermentation condition and the solid medium supplemented with urea and NH4NO3, CoSO4, Tween 80, 1% glucose, CXN2-3A produced RSDE 19.23 folds higher than wild type cultured in pre-optimized condition and un-supplemented medium. Chủng nấm chọn lọc sản xuất enzyme thủy phân tinh bột bằng cách lên men trạng thái rắn, chủng nấm được cải thiện bằng chiếu xạ tia cực tím, tia Co60 và các phương pháp xử lí với N-methyl-N'-nitrosoguanidine. Mutant Aspergillus sp. CXN2-3A, đã được lựa chọn để sản xuất enzyme (RSDE) thủy phân tinh bột sống cải thiện cao hơn 2 lần so với chủng dại. Điều kiện tối ưu cho việc sản xuất các enzyme bằng cách sử dụng cám, lúa mì đã được thực hiện cho CXN2-3A. Với điều kiện lên men xốp tối ưu và bổ sung urê và NH4NO3, CoSO4, Tween 80, 1% glucose, CXN2-3A đã sản xuất RSDE cao gấp 19,23 lần so với kiểu dại ở cùng điều kiện.

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Production of raw-starch digesting amyloglucosidase by Aspergillus sp GP-21 in solid state fermentation

Journal of Industrial Microbiology & Biotechnology ◽

10.1038/sj.jim.2900676 ◽

1999 ◽

Vol 22 (6) ◽

pp. 622-626 ◽

Cited By ~ 21

Author(s):

G Mamo ◽

A Gessesse

Keyword(s):

Solid State ◽

Solid State Fermentation ◽

Raw Starch ◽

Aspergillus Sp

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Biotransformation of Animal Fat-By Products into ARA-Enriched Fermented Bioproducts by Solid-State Fermentation of Mortierella alpina

Journal of Fungi ◽

10.3390/jof6040236 ◽

2020 ◽

Vol 6 (4) ◽

pp. 236 ◽

Cited By ~ 1

Author(s):

Ondrej Slaný ◽

Tatiana Klempová ◽

Volha Shapaval ◽

Boris Zimmermann ◽

Achim Kohler ◽

...

Keyword(s):

Solid State ◽

Wheat Bran ◽

Solid State Fermentation ◽

Fatty Acid Content ◽

Maximum Yield ◽

Mortierella Alpina ◽

Cell Factory ◽

Animal Fat ◽

Wide Range ◽

By Products

Solid-state fermentation (SSF) is a powerful fermentation technology for valorizing rest materials and by-products of different origin. Oleaginous Zygomycetes fungi are often used in SSF as an effective cell factory able to valorize a wide range of hydrophilic and hydrophobic substrates and produce lipid-enriched bioproducts. In this study, for the first time, the strain Mortierella alpina was used in SSF for the bioconversion of animal fat by-products into high value fermented bioproducts enriched with arachidonic acid (ARA). Two cereals-based matrixes mixed with four different concentrations of animal fat by-product were evaluated for finding optimal conditions of a fat-based SSF. All obtained fermented bioproducts were found to be enriched with ARA. The highest substrate utilization (25.8%) was reached for cornmeal and it was almost double than for the respective wheat bran samples. Similarly, total fatty acid content in a fermented bioproduct prepared on cornmeal is almost four times higher in contrast to wheat bran-based bioproduct. Although in general the addition of an animal fat by-product caused a gradual cessation of ARA yield in the obtained fermented bioproduct, the content of ARA in fungal biomass was higher. Thus, M. alpina CCF2861 effectively transformed exogenous fatty acids from animal fat substrate to ARA. Maximum yield of 32.1 mg of ARA/g of bioproduct was reached when using cornmeal mixed with 5% (w/w) of an animal fat by-product as substrate. Furthermore, implementation of attenuated total reflectance Fourier transform infrared (ATR-FTIR) spectroscopy in characterization of obtained SSF bioproducts was successfully tested as an alternative tool for complex analysis, compared to traditional time-consuming methods.

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Growth temperature of different local isolates of Bacillus sp. in the solid state affects production of raw starch digesting amylases

Archives of Biological Sciences ◽

10.2298/abs1402483s ◽

2014 ◽

Vol 66 (2) ◽

pp. 483-490

Author(s):

Marinela Sokarda-Slavic ◽

Natasa Bozic ◽

Z. Vujcic

Keyword(s):

Solid State ◽

Amylase Activity ◽

Agar Plate ◽

Bacillus Sp ◽

Wild Type ◽

Raw Starch ◽

Total Enzyme Activity ◽

Hydrolysis Of ◽

Type Strains ◽

Total Enzyme

Natural amylase producers, wild type strains of Bacillus sp., were isolated from different regions of Serbia. Strains with the highest amylase activity based on the starch-agar plate test were grown on solid-state fermentation (SSF) on triticale. The influence of the substrate and different cultivation temperature (28 and 37?C) on the production of amylase was examined. The tested strains produced ?-amylases when grown on triticale grains both at 28 and at 37?C, but the activity of amylases and the number and intensity of the produced isoforms were different. Significant hydrolysis of raw cornstarch was obtained with the Bacillus sp. strains 2B, 5B, 18 and 24B. The produced ?-amylases hydrolyzed raw cornstarch at a temperature below the temperature of gelatinization, but the ability for hydrolysis was not directly related to the total enzyme activity, suggesting that only certain isoforms are involved in the hydrolysis.

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Solid State Fermentation of a Raw Starch Digesting Alkaline Alpha-Amylase from Bacillus licheniformis RT7PE1 and Its Characteristics

Biotechnology Research International ◽

10.1155/2014/495384 ◽

2014 ◽

Vol 2014 ◽

pp. 1-8 ◽

Cited By ~ 7

Author(s):

Romana Tabassum ◽

Shazia Khaliq ◽

Muhammad Ibrahim Rajoka ◽

Foster Agblevor

Keyword(s):

Molecular Weight ◽

Solid State ◽

Bacillus Licheniformis ◽

Wheat Bran ◽

Solid State Fermentation ◽

Half Life ◽

Alpha Amylase ◽

Kinetic Properties ◽

Optimum Temperature ◽

Raw Starch

The thermodynamic and kinetic properties of solids state raw starch digesting alpha amylase from newly isolated Bacillus licheniformis RT7PE1 strain were studied. The kinetic values Qp, Yp/s, Yp/X, and qp were proved to be best with 15% wheat bran. The molecular weight of purified enzyme was 112 kDa. The apparent Km and Vmax values for starch were 3.4 mg mL−1 and 19.5 IU mg−1 protein, respectively. The optimum temperature and pH for α-amylase were 55°C, 9.8. The half-life of enzyme at 95°C was 17h. The activation and denaturation activation energies were 45.2 and 41.2 kJ mol−1, respectively. Both enthalpies (ΔH∗) and entropies of activation (ΔS∗) for denaturation of α-amylase were lower than those reported for other thermostable α-amylases.

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Production of Lovastatin by Wild Strains of Aspergillus terreus

Natural Product Communications ◽

10.1177/1934578x1100600207 ◽

2011 ◽

Vol 6 (2) ◽

pp. 1934578X1100600 ◽

Cited By ~ 2

Author(s):

Ravindra H. Patil ◽

Prakash Krishnan ◽

Vijay L. Maheshwari

Keyword(s):

Candida Albicans ◽

Solid State ◽

Wheat Bran ◽

Aspergillus Terreus ◽

Fungal Strain ◽

Raw Material ◽

Rice Husks ◽

Dna Analyses ◽

Pellet Formation ◽

Wild Strains

A wild fungal strain of Aspergillus terreus, labeled as PM3, was isolated by using the Candida albicans bioassay and confirmed by 18S r DNA analyses. Lovastatin was produced by submerged and solid state fermentations. Of the 30 isolated fungal strains, 11 showed lovastatin production with Aspergillus terreus PM3 being the best with a yield of 240 mg/L at the 10th day of submerged fermentation. Carboxymethylcellulose had a stimulatory effect on lovastatin production. It restricted uncontrolled filamentous growth, induced pellet formation and, thereby, improved lovastatin yield. In solid state fermentation (SSF), of the agro wastes from five crops (bran of wheat and rice, husks of red gram and soybean, and green gram straw), wheat bran showed maximum lovastatin production (12.5 mg/g of dry substrate) at pH 7.1 and a temperature of 30±2°C. Development of a lovastatin production process based on wheat bran as a substrate in SSF is economically attractive as it is a cheap and readily available raw material in agriculture-based countries.

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Improvement of carboxymethyl cellulase production from Chaetomium cellulolyticum NRRL 18756 by mutation and optimization of solid state fermenttion

Bangladesh Journal of Botany ◽

10.3329/bjb.v40i2.9769 ◽

1970 ◽

Vol 40 (2) ◽

pp. 139-147 ◽

Cited By ~ 6

Author(s):

Eman M Fawzi ◽

Hossam S Hamdy

Keyword(s):

Solid State ◽

Solid State Fermentation ◽

Industrial Process ◽

Tween 80 ◽

Cellulase Production ◽

Potential Candidate ◽

Wild Type ◽

Carboxymethyl Cellulase ◽

Optimal Moisture Content ◽

Initial Ph

Cellulase producing fungus Chaetomium cellulolyticum NRRL 18756 was subjected to various doses of gamma irradiation to enhance the production of the industrially important enzyme carboxymethyl cellulase (CMCase). Among all the mutants tested, M-7 obtained through 0.5 KGy irradiation showed highest extracellular CMCase production which is 1.6-fold higher than that of the wild type. Optimal conditions for the production of CMCase by the mutant fungal strain using solid-state fermentation were examined. The optimized medium consisted of sugarcane bagasse supplemented with 1% (w/w) peptone, 2.5mM MgSO4, and 0.05% (v/w) Tween 80. Optimal moisture content and initial pH was 40% (v/w) and 5.0-6.5, respectively. The medium was fermented at 40° C for 4 days. The resulting CMCase yield was 4.0-fold more than that of the wild type strain grown on the basal wheat bran medium. Some characteristics of partially purified CMCase from the mutant and wild type of C. cellulolyticum were investigated. The partially purified mutant CMCase was more stable than the wild type CMCase. Thus, the higher thermostability of mutant CMCase makes it a potential candidate for commercial and industrial process. Key words: Chaetomium cellulolyticum; Carboxymethyl cellulase; Mutation; Optimization; Solid state fermentation DOI: http://dx.doi.org/10.3329/bjb.v40i2.9769 Bangladesh J. Bot. 40(2): 139-147, 2011 (December)

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