scholarly journals Effects of ammonia toxicity on growth performance, cortisol, glucose and hematological response of Nile Tilapia (Oreochromis niloticus)

2016 ◽  
Vol 1 (1) ◽  
pp. 21-28 ◽  
Author(s):  
Moustafa M. Zeitoun ◽  
Kheir El-Din M. EL-Azrak ◽  
Mohamed A. Zaki ◽  
Bahig R. Nemat-Allah ◽  
El-Sayed E. Mehana
Keyword(s):  
Growth Rate ◽  
Growth Performance ◽  
Ammonium Chloride ◽  
Hematological Parameters ◽  
Hemoglobin Concentration ◽  
Mean Corpuscular Hemoglobin ◽  
Fish Production ◽  
Corpuscular Hemoglobin ◽  
Male And Female ◽  
Males And Females

Ammonia is a production limiting factor in the aquaculture media affecting fish production. A study was designed to scrutinize effects of ammonia on growth performance, survival, cortisol and hematological parameters of Tilapia fish. The study examined effects of 96 h-incubation of male and female Tilapia with 3 mg ammonium chloride per a liter of water compared to control. The study has been carried out in the physiology laboratory of the department of animal and fish production, Alexandria University. Fourteen aquaria were used (6 control and 8 ammoniated). Each aquarium contained 6 fish (half the population males and the other have females). Duration of the control reared fish was 30 days, however the duration for ammoniated group was 4 days. In all stressed fish, there found decreases in final body weight, average daily gain and specific growth rate as compared to controls. Hematological parameters revealed increases (P0.05) in total leukocyte counts in both males and females exposed to stressors. There were significant decreases (P0.05) in red blood cell, hematocrit value and hemoglobin concentration in both males and females. There were non-significant differences (P0.10) in these parameters between males and females. Exposing both male and female tilapia to ammonia, resulted in increases (P0.05) in mean corpuscular volume (MCV). Mean corpuscular hemoglobin (MCH) didn’t change in male tilapia, while females expressed increased MCH values in the ammonia condition. Mean corpuscular hemoglobin concentration (MCHC) decreased (P0.05) under ammonia with no differences between males and females. Differential leukocyte count exhibited increases (P0.05) in neutrophils in ammonia-exposed males and females and decreases (P0.05) in eosinophils and monocytes in males, but not in females. However, lymphocytes decreased (P0.05) in both females and males exposed to ammonia. Cortisol level increased (P0.05) by about 2 folds in both sexes of fish exposed to ammonia (2.95 and 2.72 vs6.40 and 6.48 ng/ml in control males and females vs ammonia-exposed males and females).Rearing tilapia fish in media containing high level (3mg/l water) of ammonium chloride not only deteriorated growth rate but it also negatively affected the health wellbeing. 

scholarly journals Blood Donation in Nigeria: Standard of the Donated Blood

2012 ◽  
Vol 4 (02) ◽  
pp. 094-097 ◽  
Author(s):  
Nwogoh Benedict ◽  
Awodu Omolade Augustina ◽  
Bazuaye Godwin Nosakhare
Keyword(s):  
Blood Donation ◽  
Hematological Parameters ◽  
Hemoglobin Concentration ◽  
Mean Corpuscular Hemoglobin ◽  
Corpuscular Hemoglobin ◽  
Mean Cell Volume ◽  
Benin City ◽  
Cell Counts ◽  
Significant Difference ◽  
The Mean

ABSTRACT Objective: The objective was to determine the basic hematological parameters of remunerated blood donors in Benin City and to compare them with those of voluntary donors. Materials and Methods: This is a prospective study conducted in a tertiary health facility in Benin City. Pretransfusion samples were obtained from blood bags after gentle mixing and analyzed for hematological parameters. Samples were analyzed using the hematology autoanalyzer MODEL SYSMEX KN21. Result: A total of 215 samples were obtained comprising 160 remunerated (paid) and 55 voluntary donor samples. In the paid donors, the mean hemoglobin concentration (Hb) and hematocrit (HCT) 7.7±2.9 and 28.8±8.5 respectively. This was significantly lower than those of voluntary donors who had 13.9±1.2 and 42.2±3.3 with P < 0.001. The mean values of the red cell counts (RBC), white cell counts (WBC), mean cell volume (MCV), mean corpuscular hemoglobin (MCH), and mean corpuscular hemoglobin concentration (MCHC) were significantly lower in paid donors as P-values were <0.001. MCV was significantly low but not compared to the other parameters as P=0.04. There was no significant difference in the platelet count. Conclusion: Paid donors in Benin City have significantly lower hematological parameters than controls.

scholarly journals Statin and anemia in chronic kidney disease (CKD): an experimental study

2018 ◽  
Vol 197 ◽  
pp. 07003 ◽  
Author(s):  
P N Cahyawati ◽  
A A S A Aryastuti ◽  
M B T Ariawan ◽  
N Arfian ◽  
Ngatidjan Ngatidjan
Keyword(s):  
Chronic Kidney Disease ◽  
Kidney Disease ◽  
Hematological Parameters ◽  
Treatment Options ◽  
White Blood Cells ◽  
Hemoglobin Concentration ◽  
Mean Corpuscular Hemoglobin ◽  
Corpuscular Hemoglobin ◽  
Simvastatin Group ◽  
Group 5

Anemia is a common feature of chronic kidney disease (CKD). The current management of patients with anemia in CKD is controversial. These make anemia is one of the leading causes poor outcome in CKD patients. Our study focus to assess the effect of statin on hemoglobin level with an experimental model of CKD. We perform the animal model of CKD by subtotal nephrectomy procedure. A total of 20 male swiss was used in these study. All the animal divided into 4 groups: sham group (S), nephrectomy (Nx), simvastatin group 5 mg/kgBB (SV5), and simvastatin group 10 mg/kgBB (SV10). Hematological parameters (hemoglobin (Hb), white blood cells (WBC), red blood cell (RBC), hematocrit (HCT), mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC)), and serum creatinine are measured at the end of the study. The hemoglobin levels in the Ux group decreased significantly compared with the S group (11.02 ± 0.46 vs 14.3 ± 0.15, p<0.0001). While in the SV group, statin administration led to an increase in hemoglobin levels compared with the Ux group but not statistically significant (SV5: 13.26±0.34; Sv10: 12.68±0.26). These results suggest that administration of statins in CKD may improve the condition of anemia by an unknown mechanism, while still requiring other treatment options to maximize the effect of therapy.

scholarly journals Hematology of Wild Caught Dubois’s Tree FrogPolypedates teraiensis, Dubois, 1986 (Anura: Rhacophoridae)

2014 ◽  
Vol 2014 ◽  
pp. 1-7 ◽  
Author(s):  
Madhusmita Das ◽  
Pravati Kumari Mahapatra
Keyword(s):  
Blood Cell ◽  
Blood Serum ◽  
Blood Cells ◽  
Hematological Parameters ◽  
Hemoglobin Concentration ◽  
Mean Corpuscular Hemoglobin ◽  
Reference Ranges ◽  
Corpuscular Hemoglobin ◽  
Differential Leukocyte Count ◽  
Rbc Count

Blood was analyzed from eighty (forty males and forty females) adult individuals ofPolypedates teraiensisto establish reference ranges for its hematological and serum biochemical parameters. The peripheral blood cells were differentiated as erythrocytes, lymphocytes, eosinophils, neutrophils, monocytes, basophils, and thrombocytes, with similar morphology to other anurans. Morphology of blood cells did not vary according to sex. The hematological investigations included morphology and morphometry of erythrocytes, morphometry of leucocytes, packed cell volume (PCV), hemoglobin content (Hb), mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC), erythrocyte or red blood cell (RBC) count, leukocyte or white blood cell (WBC) count, differential leukocyte count, and neutrophil to lymphocyte (N/L) ratio. Besides, protein, cholesterol, glucose, urea, uric acid, and creatinine content of blood serum were assayed. Hematological parameters that differed significantly between sexes were RBC count, length and breadth of RBC, neutrophil %, N/L ratio, area occupied by basophils, and diameter of large lymphocyte and eosinophils. The level of glucose, urea, and creatinine in blood serum also significantly differed between sexes.

scholarly journals Hematological parameters of Hoplias malabaricus (Characiformes: Erythrinidae) parasitized by Monogenea in lagoons in Pirassununga, Brazil

2013 ◽  
Vol 22 (4) ◽  
pp. 457-462 ◽  
Author(s):  
Lincoln Lima Corrêa ◽  
Letícia Cucolo Karling ◽  
Ricardo Massato Takemoto ◽  
Paulo Sérgio Ceccarelli ◽  
Marlene Tiduko Ueta
Keyword(s):  
Condition Factor ◽  
Hematological Parameters ◽  
Hemoglobin Concentration ◽  
Good Health ◽  
Blood Parameters ◽  
Mean Corpuscular Hemoglobin ◽  
Corpuscular Hemoglobin ◽  
Hoplias Malabaricus ◽  
Relative Condition Factor ◽  
The Mean

Hematology is an important pathological and diagnostic tool. This paper describes the hematological parameters of 76 specimens of Hoplias malabaricus infested with monogenean parasites, which were collected from two lagoons. The hematological parameters included: erythrocyte count (Er), microhematocrit (Hct), hemoglobin (Hb), mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH) and mean corpuscular hemoglobin concentration (MCHC). The blood parameters showed no significant changes associated with the infestation. Water temperature was found to be negatively correlated with MCV and Hct, with values of rs= −0.52, p<0.0001 and rs= −0.48, p<0.0001, respectively. The mean Relative Condition Factor was Kn=1.01, indicating good health conditions of the fish in these lagoons. No correlation was found between the monogenean infestation and the blood parameters, or between the environmental factors and the monogeneans.

scholarly journals Hematological parameters in Nile Tilápia, Oreochromis niloticus exposed to sub-letal concentrations of mercury

2007 ◽  
Vol 50 (4) ◽  
pp. 619-626 ◽  
Author(s):  
Nilton Massuo Ishikawa ◽  
Maria José Tavares Ranzani-Paiva ◽  
Julio Vicente Lombardi ◽  
Cláudia Maris Ferreira
Keyword(s):  
Blood Cell ◽  
Cell Count ◽  
Oreochromis Niloticus ◽  
Hematological Parameters ◽  
Hemoglobin Concentration ◽  
Control Group ◽  
Mean Corpuscular Hemoglobin ◽  
Blood Cell Count ◽  
Corpuscular Hemoglobin ◽  
Set Up

Mercury toxicity in tilapia, Oreochromis niloticus, (Linnaeus, 1758) was investigated by the hematological parameters after long-term (14 days) exposure to various Hg concentrations (0.02, 0.002, 0.0002mg/L Hg). Test groups were set up with three replicates for each concentration, plus the control group. Blood samples were collected from six individuals for each concentration at 0, 3, 7, 10 and 14 days of exposure. The hematological parameters analyzed were: total red blood cell count (RBC), hemoglobin concentration (Hb), hematocrit (Ht), mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC), total white blood cell count (WBC) and differential leukocyte counts and total thrombocyte count (Tr). There were no significant differences among the mean hematological values at the different Hg concentrations indicating that Hg at the concentrations studied was not toxic to tilapia.

scholarly journals Is erythrocyte size a strategy to avoid hypoxia in Wiegmann’s Torquate Lizards (Sceloporus torquatus)? Field evidence

2015 ◽  
Vol 93 (5) ◽  
pp. 377-382 ◽  
Author(s):  
Juan C. González-Morales ◽  
Erendira Quintana ◽  
Hector Díaz-Albiter ◽  
Palestina Guevara-Fiore ◽  
Victor Fajardo
Keyword(s):  
Physiological Response ◽  
Hematological Parameters ◽  
Hemoglobin Concentration ◽  
Mean Corpuscular Hemoglobin ◽  
Mean Corpuscular Hemoglobin Concentration ◽  
Corpuscular Hemoglobin ◽  
Serum Electrolytes ◽  
Blood Samples ◽  
Field Evidence ◽  
Different Altitudes

This study examined changes in certain hematological parameters in a reptilian model naturally exposed to altitude-associated hypoxia. Four populations of the Mexican lizard Sceloporus torquatus Wiegmann, 1828 (Wiegmann’s Torquate Lizard) from different altitudes were sampled to evaluate erythrocyte count (Erc), hematocrit (Hct), mean corpuscular hemoglobin concentration (MCHC), and erythrocyte size (Ers). Blood was also assayed to determine hemoglobin ([Hb]), glucose, lactate, and electrolyte concentrations. Erc was performed using a Neubauer hemocytometer. Hct was calculated as percentage of packed cell volume by centrifuging blood samples. [Hb] was determined using a Bausch and Lomb Spectronic colorimeter. MCHC was calculated with the formula 100 × [Hb]/Hct. Ers was calculated from blood smear microphotographs analyzed with the Sigma Scan Pro software. Values of serum electrolytes (sodium (Na+), potassium (K+), and calcium (Ca2+)), pH, glucose, and lactate from blood samples were obtained through a blood electrolyte analyzer. Highland populations of S. torquatus exhibited a significant increase in Erc, Hct, Ers, and [Hb]. In contrast, MCHC showed no correlation with altitude. Additionally, significant differences in lactate, Na+, K+, and Ca2+ were observed in highland populations. In general, we found that most hematological parameters were significantly different among lizard populations from different altitudes. This is the first study to report changes in Ers in relation to altitude, which could be a physiological response to hypoxia.

scholarly journals Impact of Camel's Milk on Aluminum Chloride (Alcl3) - Induced Toxicity in Rats

2019 ◽  
Vol 16 (3) ◽  
pp. 669-679
Author(s):  
Hala A. Abdolhaleem ◽  
Magda Abd Elaziz ◽  
Mostafa M. Bashandy ◽  
Wafai Z. A. Mikhail
Keyword(s):  
Aluminum Chloride ◽  
Histopathological Examination ◽  
Hematological Parameters ◽  
Hemoglobin Concentration ◽  
Negative Control ◽  
Camel Milk ◽  
Mean Corpuscular Hemoglobin ◽  
Corpuscular Hemoglobin ◽  
Renal Functions ◽  
Camel's Milk

The present study was carried out to evaluate the efficiency of camel's milk to ameliorate the toxicity of aluminum chloride AlCl3 on some hematological parameters; hepatic,renal functions andlipids profile; as well ashistopathological alterations of some organs. Forty rats (8 / group) were divided into 5 treatment groups:Group1: Normal rats (negative control); Group2: AlCl3induced toxicity rats (positive control); Group3: AlCl3induced toxicity rats fed with raw camel milk; Group4: AlCl3 induced toxicity rats fed with heat treated camel milk; andGroup5: AlCl3 induced toxicity rats fed with sweet acidophilus camel milk.Rats were treatedby 5ml camel’s milk 10 min before the administration of 1 ml AlCl3 (0.5 mg / kgbody weight); and had their respective doses daily for 30 successive days orally. AlCl3 oral administration resulted in a significant decrease in red blood cells count (RBC's), significant increase in mean corpuscular volume (MCV) and mean corpuscular hemoglobin (MCH); while hemoglobin (Hb), hematocrite (Hct), platelets(plt), reticulocytes (Ret), mean corpuscular hemoglobin concentration (MCHC) did not revealed significant changes; the obtained anemia was macrocytic normochromic. The lipids profile; hepatic and renal functions showed non significant changes between different groups; however, histopathological examination showed variable alterations of varying severity in some organs; besides their response to camel's milk administration. Camel’s milk administration in groups 3, 4, 5 alleviated the toxic effect of AlCl3 with variable degrees between different groups.

scholarly journals BIỂU HIỆN LÂM SÀNG VÀ MỘT SỐ CHỈ TIÊU SINH LÝ MÁU CỦA LỢN MẮC DỊCH TIÊU CHẢY CẤP (PORCINE EPIDEMIC DIARRHEA - PED) NUÔI TẠI TỈNH THANH HOÁ

2020 ◽  
Vol 4 (2) ◽  
pp. 1928-1932
Author(s):  
Lê Văn Thái ◽  
Nguyễn Đình Hiệp ◽  
Lương Trọng Thắng
Keyword(s):  
Hematological Parameters ◽  
Clinical Manifestations ◽  
Hemoglobin Concentration ◽  
Pulse Frequency ◽  
White Blood Count ◽  
Mean Corpuscular Hemoglobin ◽  
Mean Corpuscular Hemoglobin Concentration ◽  
Corpuscular Hemoglobin ◽  
Study Results ◽  
Porcine Epidemic Diarrhea

Nghiên cứu nhằm xác định những biểu hiện lâm sàng và một số đặc điểm sinh lý máu của lợn mắc hội chứng tiêu chảy cấp (Porcine Epidemic Diarrhea - PED) để so với lợn khỏe nuôi tại một số trang trại thuộc tỉnh Thanh Hóa. Kết quả nghiên cứu cho thấy: khi lợn mắc dịch PED tần số hô hấp và tần số mạch cao hơn (45,13 và 138,19 lần/phút so với 23,74 và 95,31 lần/phút) trong khi thân nhiệt lại thấp hơn so với lợn khỏe (37,50 và 38,170C). Số lượng hồng cầu của lợn mắc PED và lợn khỏe không sai khác có ý nghĩa thống kê (6,58 - 6,61 triệu/ mm3). Trong khi, các chỉ tiêu như hemoglobin, lượng hemoglobin trung bình trong một hồng cầu (MCH), nồng độ hemoglobin trung bình (MCHC) của lợn khỏe đều cao hơn so với lợn mắc PED. Tổng số bạch cầu và tỷ lệ các loại bạch cầu trung tính cao hơn so với lợn khỏe. ABSTRACT The study was conducted to determine clinical manifestations and some blood hematological parameters of pigs infected with Porcine Epidemic Diarrhea (PED) to compare with healthy pigs raised in Thanh Hoa province. The research results showed that respiratory and pulse frequency of pigs infected with PED was higher than healthy pigs. In addition, their body temperature was lower than healthy pigs were. The number of red blood cells of pigs infected with PED and healthy pigs was from 6.58 to 6.61 (106/ mm3). Hemoglobin, mean corpuscular hemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC) in healthy pigs were higher than PED pigs. Total of white blood count and neutrophils in PED pigs was higher than healthy pigs. The study results are the basis of the initial prognosis for diagnosing PED disease  that would be the reference for researchers of livestock and veterinary.  

scholarly journals AMELIORATIVE EFFECT OF NARINGENIN AGAINST ANTITUBERCULOSIS DRUGS INDUCED ALTERATIONS IN HEMATOLOGICAL PARAMETERS OF RATS

2018 ◽  
Vol 11 (11) ◽  
pp. 253 ◽  
Author(s):  
Nisha Sahu ◽  
Gita Mishra ◽  
Hemeshwer Kumar Chandra ◽  
Satendra Kumar Nirala ◽  
Monika Bhadauria
Keyword(s):  
Hematological Parameters ◽  
Hemoglobin Concentration ◽  
Hematological Parameter ◽  
Mean Corpuscular Hemoglobin ◽  
Corpuscular Hemoglobin ◽  
Antituberculosis Drugs ◽  
Hematological Indices ◽  
Significant Reversal ◽  
Ameliorative Effect ◽  
Conjoint Treatment

Objective: The aim of the present study was to evaluate the efficacy of naringenin against antituberculosis drugs (ATDs) induced alteration in hematological parameters in rats.Methods: Rats were administered with ATDs for 8 weeks (3 days/weeks) followed by naringenin at three different doses (10, 20, and 40 mg/kg) conjointly for 8 weeks (3 days/weeks) orally. After 8 weeks, animals were euthanized; blood was collected by retro-orbital sinus method for the analysis of hematological parameters.Results: The results of this study show decreased in red blood cells, hemoglobin, mean corpuscular volume, mean corpuscular hemoglobin, mean corpuscular hemoglobin concentration, platelets, and eosinophils along with increase in the number of lymphocytes in ATDs induced rats. Treatment with naringenin encountered ATDs induced hematological parameter alteration which was evident by significant reversal in hematological indices toward control in dose-dependent manner.Conclusion: The present study concluded that ATDs exposure caused adverse effects in various blood components and conjoint treatment of naringenin reduced hematological alterations toward control due to antioxidant activity.

scholarly journals Hematological parameters of Iranian cichlid Iranocichla hormuzensis: Coad, 1982 (Perciformes) in Mehran River

2012 ◽  
Vol 84 (4) ◽  
pp. 943-949 ◽  
Author(s):  
Ehsan Daneshvar ◽  
Mahsa Y. Ardestani ◽  
Salar Dorafshan ◽  
Mauricio L. Martins
Keyword(s):  
Blood Cells ◽  
Mean Corpuscular Volume ◽  
High Efficiency ◽  
Hematological Parameters ◽  
White Blood Cells ◽  
Hemoglobin Concentration ◽  
Mean Corpuscular Hemoglobin ◽  
Mean Corpuscular Hemoglobin Concentration ◽  
Corpuscular Hemoglobin ◽  
Food Fish

This study describes the hematological parameters in Iranocichla hormuzensis, an Iranian freshwater cichlid important as ornamental and food fish. Forty fish were captured with seine net at Mehran river Hormozgan province, Iran. Blood was used to determine the total counts of red blood cells (RBC) and white blood cells (WBC), hematocrit, mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC) and morphometric data of erythrocytes. The Iranian fish showed lower RBC and WBC values than the other cichlids (Oreochromis niloticus, O. aureus, O. mossambicus, O. hybrid, Cichlasoma dimerus and Cichla monoculus). Hematocrit did not vary among the species, but MCV, MCH and MCHC in I. hormuzensis were higher than those for O. niloticus, O. aureus, O. hybrid, C. dimerus and C. monoculus. These differences may be related to different life habit of fish. This study suggests that I. hormuzensis is well acclimated to the environment being the first report for its hematology. It is also suggested high efficiency in oxygen transportation, and an efficient inflow of oxygen by the gills, indicating the welfare of fish on this environment.

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