Studi pendahuluan genetika populasi ikan tuna sirip kuning (thunnus albacares) dari dua populasi di laut Kepulauan Maluku, Indonesia

Abstract. Yellowfin tuna (Thunnus albacares) is a large pelagic fish that have high economic value and inhabits the Moluccas Sea, Indonesia. Tuna catches in the Moluccas sea was very high and might decrease the yellowfin tuna population in this region. The research on population genetic of yellowfin tuna is fundamental to answer the problem. This information can be used as baseline data for future management, utilization, and basis of genetic conservation. The objective of this research was to infer the genetic population structure of two populations (North Maluku and Ambon) in the Moluccas Sea, Indonesia. In total, 41 tissue samples from pectoral fins of yellowfin tuna were collected in this study (North Maluku 33 samples and Ambon 8 samples). The results showed that genetic distances were low between the two populations. Additionally, the comparison of genetic distance between the Moluccas population and Indian Ocean waters also showed no significant differences. The Fst analysis showed the high gene flow between these two populations. Furthermore, haplotype network analysis showed that these two populations were the panmixia population. The overall result showed that no refraction genetic in the yellowfin tuna population from two populations in the Moluccas Sea.Keywords: Haplotype, genetic distance, Moluccas Sea, index fixation analysis, yellowfin tuna, population genetic structure. Abstrak. Tuna sirip kuning (Thunnus albacares) merupakan jenis pelagis besar bernilai ekonomis tinggi yang hidup di perairan Laut Maluku, Indonesia. Tangkapan ikan tuna di Laut Maluku berstatus tinggi, sehingga dapat menurunkan jumlah populasi. Penelitian tentang genetika populasi ikan tuna sirip kuning penting dilakukan untuk menjawab permasalahan ini. Informasi ini dapat menjadi sumber data untuk pengelolaan, pemanfaatan dan pelestarian untuk konservasi genetik. Tujuan penelitian untuk mengetahui struktur populasi genetik pada dua populasi di Laut Maluku (Maluku Utara dan Ambon). Secara total, 41 sampel jaringan dari sirip pectoral Tuna sirip kuning dikumpulkan dalam penelitian ini (Maluku Utara 33 sampel dan Ambon 8 sampel). Hasil penelitian menemukan jarak genetik yang dekat antar kedua populasi. Perbandingan jarak genetik pada populasi Perairan Maluku dan Samudera Hindia tidak menunjukan perbedaan signifikan. Analisis fiksasi indeks (Fst) memperlihatkan aliran genetik kuat antar populasi. Analisis jaringan haplotipe menunjukan kedua populasi merupakan populasi panmiksia. Penelitian ini secara umum menunjukkan belum terjadi perubahan struktur genetik populasi ikan tuna sirip kuning pada dua populasi di Laut Maluku.Kata kunci: Haplotipe, jarak genetik, Laut Maluku, analisis fiksasi indeks, tuna sirip kuning, struktur populasi genetik.

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Genetic Population Structure of Yellowfin Tuna (Thunnus albacares) as Based Data of Fish Conservation in North Mallucas Sea

Omni-Akuatika ◽

10.20884/1.oa.2018.14.3.457 ◽

2018 ◽

Vol 14 (3) ◽

Author(s):

Nebuchadnezzar Akbar ◽

Muhammad Aris

Keyword(s):

Genetic Structure ◽

Genetic Distance ◽

Population Decline ◽

Economic Value ◽

Pairwise Comparison ◽

Secondary Data ◽

Yellowfin Tuna ◽

Genetic Population ◽

Distance Analysis ◽

Comparison Test

Tuna is a migratory species with high economic value. Utilization of tuna is increasing and growing every year. Fishing intensity of tuna in the Maluku Sea is high and can disrupt the population. The existence of the population will be threatened if not managed properly. The latest genetic information approach is necessary to prevent the population decline. The purpose of this research was to know the genetic structure of yellowfin tuna in North Maluku waters, Indonesi. Sampling was conducted in May-July 2016 in Morotai Island, Obi and Sanana, while secondary data was gathered in Ternate Island, Bacan and Ambon. A total of 72 samples were collected and analyzed. The result of the study found that the base length (bp) of the control region of mtDNA was found to be 512 bp (base pairs). the genetic distance in the nearest population is at Sanana and Obi (0.025). The results of genetic distance analysis between population found genetic similarity between Morotai-Sanana (0,021), Obi-Sanana (0,025), Obi-Morotai (0,026) and Ambon-Sanana (0,026), while the furthest genetic distance was found in Ternate-Bacan (0,040) and Ternate-Obi (0,042). The pairwise comparison test (Fst) shows a few genetic differentiation between yellowfin tuna populations. The value (Fst) of the yellowfin tuna population shows a strong gene flow between populations. The haplotype distribution shows a relationship between haplotypes in both yellowfin tuna, thus failing to show clade between different geographic locations. Unsustainable use can harm the population through genetic quality. Several approaches should be taken to support the life cycle of yellowfin tuna. The overall result shows that there has not been any change of genetic structure of yellowfin tuna in North Maluku Sea. Keywords : Haplotype, genetic distance, North Maluku, yellowfin tuna, pairwise comparison test

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Population genetic structure of Simulium degrangei (Diptera: Simuliidae) from Western Carpathians

Biologia ◽

10.2478/s11756-012-0057-2 ◽

2012 ◽

Vol 67 (4) ◽

Cited By ~ 3

Author(s):

Ladislav Jedlička ◽

Matúš Kúdela ◽

Tomáš Szemes ◽

Peter Celec

Keyword(s):

Genetic Structure ◽

Population Genetic ◽

Haplotype Network ◽

Genetic Distances ◽

Western Carpathians ◽

Mountain Range ◽

Molecular Variation ◽

Gene Encoding ◽

The Crimea ◽

Of Greece

AbstractSimulium degrangei Dorier & Grenier, 1960 was recorded in southern and central Europe and in the Crimea and Caucasus. Its distribution pattern is scattered. The variability of the mtDNA gene encoding cytochrome oxidase I was studied in populations of S. degrangei from the Western Carpathians in Slovakia and in the northern Hellenides of Greece. In the analyzed samples, 21 haplotypes were recorded, of which twenty were private and occurred in only one mountain range, but one haplotype was found in all three studied populations from the Western Carpathians. Both haplotypes from the Hellenides were private, but these were not isolated and they fit into the haplotype network of the Western Carpathians. Statistics of genetic variability, different designs of analysis of molecular variation with non-significant differences and the pair-wise genetic distances support the hypothesis that there are no differences between the analyzed populations. Similarly, using nested clade phylogeographical analysis, no genetic-geographical structure was found.

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Application of Surplus Production Model to the Yellowfin Tuna Thunnus albacares in the northern and western parts of Aceh waters

IOP Conference Series Earth and Environmental Science ◽

10.1088/1755-1315/869/1/012072 ◽

2021 ◽

Vol 869 (1) ◽

pp. 012072

Author(s):

A Rahmah ◽

I Mardhatillah ◽

A Damora ◽

M Muhammad ◽

N Nurfadillah

Keyword(s):

Economic Value ◽

Maximum Sustainable Yield ◽

Yellowfin Tuna ◽

Production Model ◽

Purse Seine ◽

Thunnus Albacares ◽

Catch Per Unit Effort ◽

Surplus Production ◽

Surplus Production Model ◽

Banda Aceh

Abstract Yellowfin tuna Thunnus albacares is one of pelagic fish that has high potential and economic value in Banda Aceh. Utilization of this resource in Banda Aceh is using purse seine units, with the number of purse seines continuously increasing. Therefore, management needs to be done so that optimal productivity can be maintained. This study discusses the estimation of catch and effort at maximum sustainable yield (MSY) of yellowfin tuna based on catch per unit effort (CPUE) and purse seine production in Banda Aceh during 2013-2018. Mathematical analysis was carried out using the equilibrium approach with the Schaefer model. The highest catch of yellowfin tuna reached 191 tons (July) and the average CPUE for yellowfin tuna was 0.796 tons/trip with CMSY of 2,482 tons/year and EMSY of 2,765 trips/year. From 2015 to 2018, the trend of biomass continued to decline and overfishing occurred during this period.

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Isozyme and RAPD variation among and within hemp dogbane (Apocynum cannabinum) populations

Weed Science ◽

10.1017/s0043174500090810 ◽

1998 ◽

Vol 46 (4) ◽

pp. 408-413 ◽

Cited By ~ 2

Author(s):

Corey V. Ransom ◽

David S. Douches ◽

James J. Kells

Keyword(s):

Genetic Variation ◽

Genetic Distance ◽

Phenotypic Variation ◽

Population Genetic ◽

Rapd Analysis ◽

Analysis Data ◽

The Other ◽

Randomly Amplified Polymorphic Dna ◽

Population Genetic Analysis ◽

Two Populations

Clonal individuals from 16 hemp dogbane populations with phenotypic variation were analyzed using isozyme and randomly amplified polymorphic DNA (RAPD) analysis. Plants originated from populations in Michigan and Illinois. Three knownApocynumspecies, spreading dogbane, hemp dogbane, and prairie dogbane, were evaluated. Genetic distance among populations was more pronounced with isozyme analysis compared to RAPD analysis. The combined isozyme and RAPD analysis data separated spreading dogbane from all other plants analyzed. Genetic variation was present among the 16 hemp dogbane populations, but was less than expected based on the phenotypic variation present among the collections. The short genetic distance between the 16 hemp dogbane collections and the threeApocynumspecies suggests that variation among populations of hemp dogbane may be from outcrossing with other closely relatedApocynumspecies. Isozyme and RAPD analyses were also conducted on plants from two populations in Michigan to determine the level of genetic variation among plants within the same population. Genetic analysis revealed that one population was entirely clonal, while the other population was a mixture of clonal and segregating plants.

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Efficiency of different PCR-based marker systems for assessment of Iris pumila genetic diversity

Biologia ◽

10.2478/s11756-013-0192-4 ◽

2013 ◽

Vol 68 (4) ◽

Cited By ~ 8

Author(s):

Olena Bublyk ◽

Igor Andreev ◽

Ruslan Kalendar ◽

Kateryna Spiridonova ◽

Viktor Kunakh

Keyword(s):

Genetic Diversity ◽

Genetic Distance ◽

Gene Diversity ◽

Issr Markers ◽

Genetic Distances ◽

Resolving Power ◽

Abiotic Stress Response ◽

Genetic Population ◽

Iris Pumila ◽

Polymorphic Bands

AbstractWe investigated informativeness and effectiveness of different marker types (ISSR, IRAP, REMAP, RGAP and LP-PCR that employ primers based on the conservative sequences of abiotic stress response genes) to study genetic diversity of Iris pumila L. By the number of amplicons per primer, number of polymorphic amplicons per primer and resolving power index (Rp), ISSR-markers were the most efficient followed by LP-PCR-markers. In order of decreasing value of indicators of genetic diversity “the percentage of polymorphic bands”, and “the average Jaccardś genetic distance between plants”, marker systems may be arranged as follows: ISSR > RAPD > LP-PC > RGAP ≈ IRAP. For ISSR-markers, the percentage of polymorphic bands was 1.3–1.7 times higher than for the others, and the average genetic distance was 1.2–1.3 times higher. Different marker systems were ranked by the value of Neiś gene diversity and the Shannonś index as follows: ISSR > RAPD ≈ LP-PCR > RGAP ≈ IRAP, with the highest and the lowest values differing 1.4 times. Genetic population structure was investigated with program Structure 2.3. The data of all marker systems suggest that all genomes under study belonged to one population. The PCoA and cluster analyses based on genetic distances showed distinctions in clustering generated from different markers data and summarized data, as well as the lack of strong clusters. Mantel test revealed significant positive correlation between the matrices of genetic distances generated by the data of almost all marker systems. The strongest correlation was found between RGAP- and IRAP-markers (r = 0.452, p = 0.01) and between RGAP and ISSR (r = 0.430, p = 0.01). ISSR, RAPD and LP-PCR proved to be more effective for the study of I. pumila genetic diversity, nevertheless, joint use of different marker systems will provide a more comprehensive assessment of variation in different genomic regions.

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Maintenance and spawning on yellowfin tuna broodstock reared in floating net cage

IOP Conference Series Earth and Environmental Science ◽

10.1088/1755-1315/890/1/012039 ◽

2021 ◽

Vol 890 (1) ◽

pp. 012039

Author(s):

Tridjoko ◽

J H Hutapea ◽

A Setiadi ◽

Gunawan ◽

B Selamet

Keyword(s):

Survival Rate ◽

Activity Index ◽

Economic Value ◽

Yellowfin Tuna ◽

Hatching Rate ◽

Thunnus Albacares ◽

Fish Feed ◽

Fresh Fish ◽

Spawning Frequency ◽

Net Cage

Abstract Yellowfin tuna (Thunnus albacares) is one of the fish species that have important economic value which is expected to become one of foreign exchange that can give solution to national economy. Hatchery technology of yellowfin tuna performed at the Research Institute for Mariculture and Fisheries Extension Gondol has been successfully spawned. However, the quality and quantity of eggs produced is still not optimal. The purpose of this study was to determine the performance of spawning on yellowfin tuna broodstock reared in floating net. This research was conducted in cage with a diameter of 48.8 m with a depth of 8 m, filled 90 fishes yellow fin tuna with weights ranging between 50-70 kg per fish. Feed given is: fresh fish, squid and added vitamin C and vitamin E. The study was conducted for 11 months from January to November 2017.The results showed that the yellowfin tuna could spawn monthly. The highest spawning frequency occurred in July at 17 times. The highest total number of eggs harvested was 8,740,350 in October. Hatching rate between 50-92%. Survival activity index (SAI) is 1.8-3.5%. Survival rate of yellow fin tuna during maintenance reached 92.2%.

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PERFORMA PEMIJAHAN IKAN TUNA SIRIP KUNING, Thunnus albacares DI KERAMBA JARING APUNG

Jurnal Riset Akuakultur ◽

10.15578/jra.12.1.2017.49-56 ◽

2017 ◽

Vol 12 (1) ◽

pp. 49 ◽

Cited By ~ 1

Author(s):

Jhon Harianto Hutapea ◽

Ananto Setiadi ◽

Gunawan Gunawan ◽

I Gusti Ngurah Permana

Keyword(s):

Water Quality ◽

Research And Development ◽

Dissolve Oxygen ◽

Activity Index ◽

Economic Value ◽

Yellowfin Tuna ◽

Thunnus Albacares ◽

Night Time ◽

Near Shore ◽

Net Cage

Ikan tuna sirip kuning merupakan komoditas ekspor yang bernilai ekonomis tinggi yang populasinya semakin menurun di alam. Penelitian ini dilaksanakan di Balai Besar Penelitian dan Pengembangan Budidaya Laut, Gondol dan bertujuan untuk mengetahui performa pemijahan ikan tuna sirip kuning yang dipelihara di dalam keramba jaring apung. Sebanyak 100 ekor induk ikan tuna dengan ukuran bobot sekitar 15-30 kg dipelihara dalam keramba sejak tahun 2014. Induk ikan diberi pakan berupa ikan layang dan cumi-cumi dengan rasio 2:1 dua kali sehari (pagi dan sore hari). Pengamatan yang dilakukan meliputi tingkah laku induk, pemijahan, dan keragaan telur yang dihasilkan, serta kualitas air terutama suhu dan oksigen dilakukan setiap hari. Induk ikan memijah untuk pertama kalinya terjadi pada tahun 2015. Selanjutnya pemijahan terjadi hampir setiap malam hari dengan jumlah telur yang dapat dikumpulkan berkisar 30.000-3.600.000 butir. Daya tetas telur yang diperoleh berkisar 26%-96%, dengan ketahanan hidup larva tanpa pakan (survival activity index-SAI) berkisar 0,1-3,8. Berdasarkan hasil pengamatan ini dapat disimpulkan bahwa induk ikan tuna sirip kuning umur dua tahun dapat memijah di keramba jaring apung dan menghasilkan performa pemijahan yang baik.Yellowfin tuna is an export commodity and high economic value but its population tend to decrease. The research was conducted at Institute for Mariculture Research and Development of Indonesia. The purpose of this study was to observe the spawning performance of yellowfin tuna in floating net cage. The study was started in 2014 using 100 broodstock with estimated weight range of 15-30 kg. Feed supplied for broodstock were scad mackerel and squid with 2:1 ratio and was given twice a day in the morning and afternoon. The observations included broodstock behavior, spawning, egg performance, and daily morning of water quality (temperature and dissolve oxygen). First spawning was observed in January, 2015, where eggs were found in floating net collector deployed in floating net cage. Spawning occured nearly every day at night time. The number of eggs collected was ranged from 30,000 to 3,600,000 eggs with varied hatching of 26%-96% and survival activity index of 0.1-3.8. Based on these results, it can be concluded that yellowfin tuna broodstock can spawn in floating net cage near shore at the age of two year with good spawning performance.

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Advertisement call and genetic structure conservatism : good news for an endangered Neotropical frog

10.7287/peerj.preprints.1666v1 ◽

2016 ◽

Author(s):

Lucas R Forti ◽

William P Costa ◽

Lucas B Martins ◽

Carlos H L Nunes-de-Almeida ◽

Luís Felipe Toledo

Keyword(s):

Genetic Distance ◽

Haplotype Network ◽

Genetic Distances ◽

Good News ◽

Habitat Isolation ◽

Advertisement Call ◽

Phenotypic Traits ◽

Negative Effects ◽

Amphibian Species ◽

Anthropogenic Habitat

Background. Many amphibian species are negatively affected by anthropogenic habitat change. Populations distributed over modified landscapes may be subject to local extinction or may be relegated to remaining, likely isolated and possibly degraded, patches of available habitat. Isolation without gene flow can lead to variability in phenotypic traits due to differences in local selective pressures, such as environmental structure, microclimate or site-specific species assemblages. Methods. Here we tested this microevolution hypothesis by evaluating the acoustic parameters of 349 advertisement calls from 15 males representing six populations of the endangered species Proceratophrys moratoi. In addition, we analyzed the genetic distance between populations and the genetic diversity by haplotype network. We carried out a cluster analysis by Bray-Curtis index of similarity using acoustic data by UPGMA method. We correlated acoustic dissimilarities (calculated by Euclidean distance) with geographic and genetic distances among populations. Results. Spectral traits of the advertisement call of P. moratoi presented lower coefficients of variation than did temporal traits both within and among males. Cluster analyses placed individuals without population and geographical distance congruence, but recovered the species topology in relation to sister species. The genetic distance was low among populations: it did not exceed 0.4% for the most distant populations, and was not correlated with acoustic distance. Discussion. Both acoustic features and genetic sequences are highly conserved, suggesting that populations may be connected by recent migrations or that they are subject to stabilizing selective forces. Although future studies are needed, these findings contribute to a growing body of literature suggesting that this species would be a good candidate for a reintroduction program without negative effects on communication or even genetic heritage.

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