Background-free Detection of Mouse Antibodies on Mouse Tissue by Anti-isotype Secondary Antibodies: Mouse on Mouse Ab Staining
Keyword(s):
Immunodetection on mouse routinely processed tissue via antibodies raised in mice faces cross-reactivity of the secondary anti-mouse reagents with endogenous immunoglobulins, which permeate the tissue. Various solutions to this problem have been devised and include endogenous Ig block with anti-mouse Fab fragments or directly conjugated primary antibodies. Mouse isotype-specific antibodies, differently from reagents directed against both heavy and light chains, fail to detect endogenous Ig after fixation and embedding, while providing a clean and specific detection system for mouse antibodies on mouse routinely processed tissue.
1992 ◽
Vol 40
(9)
◽
pp. 1319-1328
◽
1996 ◽
Vol 42
(12)
◽
pp. 1915-1923
◽
Keyword(s):
1966 ◽
Vol 123
(5)
◽
pp. 921-934
◽
Keyword(s):
2009 ◽
Vol 91B
(1-6)
◽
pp. 27-33