scholarly journals Neuron tracing and quantitative analyses of dendritic architecture reveal symmetrical three-way-junctions and phenotypes of git-1 in C. elegans

2021 ◽  
Vol 17 (7) ◽  
pp. e1009185
Author(s):  
Omer Yuval ◽  
Yael Iosilevskii ◽  
Anna Meledin ◽  
Benjamin Podbilewicz ◽  
Tom Shemesh
Keyword(s):  
Feature Extraction ◽  
Deep Learning ◽  
Dendritic Morphology ◽  
Control Mechanisms ◽  
Structural Elements ◽  
Molecular Control ◽  
Dendritic Trees ◽  
C Elegans ◽  
Neuron Tracing ◽  
Quantitative Analyses

Complex dendritic trees are a distinctive feature of neurons. Alterations to dendritic morphology are associated with developmental, behavioral and neurodegenerative changes. The highly-arborized PVD neuron of C. elegans serves as a model to study dendritic patterning; however, quantitative, objective and automated analyses of PVD morphology are missing. Here, we present a method for neuronal feature extraction, based on deep-learning and fitting algorithms. The extracted neuronal architecture is represented by a database of structural elements for abstracted analysis. We obtain excellent automatic tracing of PVD trees and uncover that dendritic junctions are unevenly distributed. Surprisingly, these junctions are three-way-symmetrical on average, while dendritic processes are arranged orthogonally. We quantify the effect of mutation in git-1, a regulator of dendritic spine formation, on PVD morphology and discover a localized reduction in junctions. Our findings shed new light on PVD architecture, demonstrating the effectiveness of our objective analyses of dendritic morphology and suggest molecular control mechanisms.

scholarly journals Neuron tracing and quantitative analyses of dendritic architecture reveal symmetrical three-way-junctions and phenotypes of git-1 in C. elegans

2021 ◽  
Author(s):  
Omer Yuval ◽  
Yael Iosilevskii ◽  
Benjamin Podbilewicz ◽  
Tom Shemesh
Keyword(s):  
Molecular Mechanisms ◽  
Dendritic Tree ◽  
Structural Features ◽  
Dendritic Morphology ◽  
Control Mechanisms ◽  
Molecular Control ◽  
Dendritic Trees ◽  
C Elegans ◽  
Quantitative Analyses ◽  
Linear Sections

AbstractComplex dendritic trees are a distinctive feature of neurons. Alterations to dendritic morphology are associated with developmental, behavioral and neurodegenerative diseases. The highly-arborized PVD neuron of C. elegans serves as a model to study dendritic patterning; however, quantitative, objective and automated analyses of PVD morphology are missing. Here, we present a method for neuronal feature extraction, based on deep-learning and fitting algorithms. The extracted neuronal architecture is represented by a database of structural elements for abstracted analysis. We obtain excellent automatic tracing of PVD trees and uncover that dendritic junctions are unevenly distributed. Surprisingly, these junctions are three-way-symmetrical on average, while dendritic processes are arranged orthogonally. We quantify the effect of mutations in git-1, a regulator of dendritic spine formation, on PVD morphology and discover a localized reduction in junctions. Our findings shed new light on PVD architecture, demonstrating the effectiveness of our objective analyses of dendritic morphology and suggest molecular control mechanisms.Author SummaryNerve cells (neurons) collect input signals via branched cellular projections called dendrites. A major aspect of the study of neurons, dating back for over 100 years, involves the characterization of neuronal shapes and of their dendritic processes.Here, we present an algorithmic approach for detection and classification of the tree-like dendrites of the PVD neuron in C. elegans worms. A key feature of our approach is to represent dendritic trees by a sets of fundamental shape elements, such as junctions and linear sections. By analyzing this dataset, we discovered several novel structural features. We have found that the junctions connecting branched dendrites have a three-way-symmetry, although the dendrites are arranged in a crosshatch pattern; and that the distribution of junctions varies across distinct sub-classes of the PVD’s dendritic tree. We further quantified subtle morphological effects due to mutations in git-1 gene, a known regulator of dendritic spines. Our findings suggest molecular mechanisms for dendritic shape regulation and may help direct new avenues of research.

Smart teaching mode based on particle swarm image recognition and human-computer interaction deep learning

2020 ◽  
Vol 39 (4) ◽  
pp. 5699-5711
Author(s):  
Shirong Long ◽  
Xuekong Zhao
Keyword(s):  
Feature Extraction ◽  
Particle Swarm Optimization ◽  
Deep Learning ◽  
Real Time ◽  
Image Recognition ◽  
Particle Swarm ◽  
Learning Technology ◽  
Search Performance ◽  
Swarm Optimization ◽  
Teaching Mode

The smart teaching mode overcomes the shortcomings of traditional teaching online and offline, but there are certain deficiencies in the real-time feature extraction of teachers and students. In view of this, this study uses the particle swarm image recognition and deep learning technology to process the intelligent classroom video teaching image and extracts the classroom task features in real time and sends them to the teacher. In order to overcome the shortcomings of the premature convergence of the standard particle swarm optimization algorithm, an improved strategy for multiple particle swarm optimization algorithms is proposed. In order to improve the premature problem in the search performance algorithm of PSO algorithm, this paper combines the algorithm with the useful attributes of other algorithms to improve the particle diversity in the algorithm, enhance the global search ability of the particle, and achieve effective feature extraction. The research indicates that the method proposed in this paper has certain practical effects and can provide theoretical reference for subsequent related research.

Multi Disease-Prediction Framework Using Hybrid Deep Learning: An Optimal Prediction Model (Preprint)

2020 ◽  
Author(s):  
Anusha Ampavathi ◽  
Vijaya Saradhi T
Keyword(s):  
Feature Extraction ◽  
Big Data ◽  
Deep Learning ◽  
Weight Function ◽  
Optimization Algorithm ◽  
Large Scale ◽  
Heuristic Algorithms ◽  
Disease Prediction ◽  
Health Care Decisions ◽  
Proposed Model

UNSTRUCTURED Big data and its approaches are generally helpful for healthcare and biomedical sectors for predicting the disease. For trivial symptoms, the difficulty is to meet the doctors at any time in the hospital. Thus, big data provides essential data regarding the diseases on the basis of the patient’s symptoms. For several medical organizations, disease prediction is important for making the best feasible health care decisions. Conversely, the conventional medical care model offers input as structured that requires more accurate and consistent prediction. This paper is planned to develop the multi-disease prediction using the improvised deep learning concept. Here, the different datasets pertain to “Diabetes, Hepatitis, lung cancer, liver tumor, heart disease, Parkinson’s disease, and Alzheimer’s disease”, from the benchmark UCI repository is gathered for conducting the experiment. The proposed model involves three phases (a) Data normalization (b) Weighted normalized feature extraction, and (c) prediction. Initially, the dataset is normalized in order to make the attribute's range at a certain level. Further, weighted feature extraction is performed, in which a weight function is multiplied with each attribute value for making large scale deviation. Here, the weight function is optimized using the combination of two meta-heuristic algorithms termed as Jaya Algorithm-based Multi-Verse Optimization algorithm (JA-MVO). The optimally extracted features are subjected to the hybrid deep learning algorithms like “Deep Belief Network (DBN) and Recurrent Neural Network (RNN)”. As a modification to hybrid deep learning architecture, the weight of both DBN and RNN is optimized using the same hybrid optimization algorithm. Further, the comparative evaluation of the proposed prediction over the existing models certifies its effectiveness through various performance measures.

scholarly journals Unsupervised Multi-Level Feature Extraction for Improvement of Hyperspectral Classification

2021 ◽  
Vol 13 (8) ◽  
pp. 1602
Author(s):  
Qiaoqiao Sun ◽  
Xuefeng Liu ◽  
Salah Bourennane
Keyword(s):  
Feature Extraction ◽  
Deep Learning ◽  
Spatial Information ◽  
Hyperspectral Data ◽  
Great Promise ◽  
Learning Models ◽  
Single Level ◽  
Multiple Networks ◽  
Multi Level ◽  
Hyperspectral Classification

Deep learning models have strong abilities in learning features and they have been successfully applied in hyperspectral images (HSIs). However, the training of most deep learning models requires labeled samples and the collection of labeled samples are labor-consuming in HSI. In addition, single-level features from a single layer are usually considered, which may result in the loss of some important information. Using multiple networks to obtain multi-level features is a solution, but at the cost of longer training time and computational complexity. To solve these problems, a novel unsupervised multi-level feature extraction framework that is based on a three dimensional convolutional autoencoder (3D-CAE) is proposed in this paper. The designed 3D-CAE is stacked by fully 3D convolutional layers and 3D deconvolutional layers, which allows for the spectral-spatial information of targets to be mined simultaneously. Besides, the 3D-CAE can be trained in an unsupervised way without involving labeled samples. Moreover, the multi-level features are directly obtained from the encoded layers with different scales and resolutions, which is more efficient than using multiple networks to get them. The effectiveness of the proposed multi-level features is verified on two hyperspectral data sets. The results demonstrate that the proposed method has great promise in unsupervised feature learning and can help us to further improve the hyperspectral classification when compared with single-level features.

Smart paddy field monitoring system using deep learning and IoT

2021 ◽  
pp. 1063293X2198894
Author(s):  
Prabira Kumar Sethy ◽  
Santi Kumari Behera ◽  
Nithiyakanthan Kannan ◽  
Sridevi Narayanan ◽  
Chanki Pandey
Keyword(s):  
Feature Extraction ◽  
Deep Learning ◽  
Transfer Learning ◽  
Paddy Field ◽  
New Technology ◽  
Support Vector ◽  
Nitrogen Status ◽  
Deep Feature ◽  
Per Capita ◽  
Deep Feature Extraction

Paddy is an essential nutrient worldwide. Rice gives 21% of worldwide human per capita energy and 15% of per capita protein. Asia represented 60% of the worldwide populace, about 92% of the world’s rice creation, and 90% of worldwide rice utilization. With the increase in population, the demand for rice is increased. So, the productivity of farming is needed to be enhanced by introducing new technology. Deep learning and IoT are hot topics for research in various fields. This paper suggested a setup comprising deep learning and IoT for monitoring of paddy field remotely. The vgg16 pre-trained network is considered for the identification of paddy leaf diseases and nitrogen status estimation. Here, two strategies are carried out to identify images: transfer learning and deep feature extraction. The deep feature extraction approach is combined with a support vector machine (SVM) to classify images. The transfer learning approach of vgg16 for identifying four types of leaf diseases and prediction of nitrogen status results in 79.86% and 84.88% accuracy. Again, the deep features of Vgg16 and SVM results for identifying four types of leaf diseases and prediction of nitrogen status have achieved an accuracy of 97.31% and 99.02%, respectively. Besides, a framework is suggested for monitoring of paddy field remotely based on IoT and deep learning. The suggested prototype’s superiority is that it controls temperature and humidity like the state-of-the-art and can monitor the additional two aspects, such as detecting nitrogen status and diseases.

scholarly journals RhoA/ROCK pathway is the major molecular determinant of basal tone in intact human internal anal sphincter

2012 ◽  
Vol 302 (7) ◽  
pp. G664-G675 ◽  
Author(s):  
Satish Rattan ◽  
Jagmohan Singh
Keyword(s):  
Anal Sphincter ◽  
Internal Anal Sphincter ◽  
Control Mechanisms ◽  
Rock Inhibitor ◽  
Critical Determinant ◽  
Molecular Control ◽  
Motility Disorders ◽  
Basal Tone ◽  
Before And After ◽  
Rational Therapy

The knowledge of molecular control mechanisms underlying the basal tone in the intact human internal anal sphincter (IAS) is critical for the pathophysiology and rational therapy for a number of debilitating rectoanal motility disorders. We determined the role of RhoA/ROCK and PKC pathways by comparing the effects of ROCK- and PKC-selective inhibitors Y 27632 and Gö 6850 (10−8to 10−4M), respectively, on the basal tone in the IAS vs. the rectal smooth muscle (RSM). Western blot studies were performed to determine the levels of RhoA/ROCK II, PKC-α, MYPT1, CPI-17, and MLC20in the unphosphorylated and phosphorylated forms, in the IAS vs. RSM. Confocal microscopic studies validated the membrane distribution of ROCK II. Finally, to confirm a direct relationship, we examined the enzymatic activities and changes in the basal IAS tone and p-MYPT1, p-CPI-17, and p-MLC20, before and after Y 27632 and Gö 6850. Data show higher levels of RhoA/ROCK II and related downstream signal transduction proteins in the IAS vs. RSM. In addition, data show a significant correlation between the active RhoA/ROCK levels, ROCK enzymatic activity, downstream proteins, and basal IAS tone, before and after ROCK inhibitor. From these data we conclude 1) RhoA/ROCK and downstream signaling are constitutively active in the IAS, and this pathway (in contrast with PKC) is the critical determinant of the basal tone in intact human IAS; and 2) RhoA and ROCK are potential therapeutic targets for a number of rectoanal motility disorders for which currently there is no satisfactory treatment.

scholarly journals Feature Extraction for Finger-Vein-Based Identity Recognition

2021 ◽  
Vol 7 (5) ◽  
pp. 89
Author(s):  
George K. Sidiropoulos ◽  
Polixeni Kiratsa ◽  
Petros Chatzipetrou ◽  
George A. Papakostas
Keyword(s):  
Feature Extraction ◽  
Deep Learning ◽  
Extraction Methods ◽  
Identity Recognition ◽  
Learning Framework ◽  
Finger Vein ◽  
The Past ◽  
Biometric Systems ◽  
Vein Recognition ◽  
Finger Vein Recognition

This paper aims to provide a brief review of the feature extraction methods applied for finger vein recognition. The presented study is designed in a systematic way in order to bring light to the scientific interest for biometric systems based on finger vein biometric features. The analysis spans over a period of 13 years (from 2008 to 2020). The examined feature extraction algorithms are clustered into five categories and are presented in a qualitative manner by focusing mainly on the techniques applied to represent the features of the finger veins that uniquely prove a human’s identity. In addition, the case of non-handcrafted features learned in a deep learning framework is also examined. The conducted literature analysis revealed the increased interest in finger vein biometric systems as well as the high diversity of different feature extraction methods proposed over the past several years. However, last year this interest shifted to the application of Convolutional Neural Networks following the general trend of applying deep learning models in a range of disciplines. Finally, yet importantly, this work highlights the limitations of the existing feature extraction methods and describes the research actions needed to face the identified challenges.

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