Specific Human Astrocyte Subtype Revealed by Affinity Purified GFAP+1 Antibody; Unpurified Serum Cross-Reacts with Neurofilament-L in Alzheimer

Objective. To investigate the effect of isoflurane (ISO) on the proliferation, apoptosis, and inflammatory response of lipopolysaccharide- (LPS-) induced normal human astrocytes (NHAs) by regulating the miR-206/BDNF axis. Methods. NHA proliferation activity was measured by MTT; NHA apoptotic rates were measured by Annexin V-FITC/PI; western blotting was used to measure the BDNF expression; ELISA was used to measure the IL-6, IL-1β, and TNF-α expression in NHAs; qPCR was used to measure the expressions of miRNAs that are related to NHAs proliferation and apoptosis; dual-luciferase reporter was constructed to validate the targeting relationship between miR-206 and BDNF. Results. LPS increased the proliferation activity and decreased the apoptosis rate of NHAs which were effectively reversed by the ISO (p<0.05); LPS significantly inhibited the expression of miRNAs related to proliferation and apoptosis in NHAs (p<0.05, p<0.01), whereas ISO significantly increased the expression of miR-206 (p<0.01) by downregulating the expression of BDNF, thus inhibiting NHA proliferation and inflammatory response and enhancing apoptosis. Conclusion. ISO can inhibit the expression of BDNF by upregulating the expression of miR-206, thereby inhibiting the proliferation and inflammatory response of NHAs and promoting its apoptosis.

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Cytokine regulation of CD40 expression in fetal human astrocyte cultures

Journal of Neuroimmunology ◽

10.1016/s0165-5728(99)00124-1 ◽

1999 ◽

Vol 101 (1) ◽

pp. 7-14 ◽

Cited By ~ 25

Author(s):

Nahed Abdel-Haq ◽

Hsiao-Nan Hao ◽

William D. Lyman

Keyword(s):

Cytokine Regulation ◽

Human Astrocyte ◽

Cd40 Expression

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gp120-induced alterations of human astrocyte function: Na+/H+exchange, K+conductance, and glutamate flux

AJP Cell Physiology ◽

10.1152/ajpcell.2000.279.3.c700 ◽

2000 ◽

Vol 279 (3) ◽

pp. C700-C708 ◽

Cited By ~ 42

Author(s):

Holly K. Patton ◽

Zhen-Hong Zhou ◽

James K. Bubien ◽

Etty N. Benveniste ◽

Dale J. Benos

Keyword(s):

Glutamate Uptake ◽

Cell Types ◽

Neurological Complications ◽

Ph Sensitive ◽

Human Astrocyte ◽

Physiological Processes ◽

Human Astrocytes ◽

Rat Astrocytes ◽

Immunodeficiency Syndrome ◽

Intracellular Alkalinization

Many human immunodeficiency virus (HIV)-infected patients suffer from impaired neurological function and dementia. This facet of the disease has been termed acquired immunodeficiency syndrome (AIDS)-associated dementia complex (ADC). Several cell types, including astrocytes and neurons, are not productively infected by virus but are involved in ADC pathophysiology. Previous studies of rat astrocytes showed that an HIV coat protein (gp120) accelerated astrocyte Na+/H+exchange and that the resultant intracellular alkalinization activated a pH-sensitive K+conductance. The present experiments were conducted to determine whether gp120 affected human astrocytes in the same fashion. It was found that primary human astrocytes express a pH-sensitive K+conductance that was activated on intracellular alkalinization. Also, gp120 treatment of whole cell clamped human astrocytes activated this conductance specifically. Furthermore, gp120 inhibited glutamate uptake by primary human astrocytes. These altered physiological processes could contribute to pathophysiological changes in HIV-infected brains. Because the gp120-induced cell physiological changes were partially inhibited by dimethylamiloride (an inhibitor of Na+/H+exchange), our findings suggest that modification of human astrocyte Na+/H+exchange activity may provide a means of addressing some of the neurological complications of HIV infection.

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Palm Fruit Bioactives modulate human astrocyte activity in vitro altering the cytokine secretome reducing levels of TNFα, RANTES and IP-10

Scientific Reports ◽

10.1038/s41598-018-34763-3 ◽

2018 ◽

Vol 8 (1) ◽

Cited By ~ 6

Author(s):

Robert P. Weinberg ◽

Vera V. Koledova ◽

Kirsten Schneider ◽

T. G. Sambandan ◽

Adlai Grayson ◽

...

Keyword(s):

Palm Fruit ◽

Human Astrocyte

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Cocaine promotes primary human astrocyte proliferation via JNK-dependent up-regulation of cyclin A2

Restorative Neurology and Neuroscience ◽

10.3233/rnn-160676 ◽

2016 ◽

Vol 34 (6) ◽

pp. 965-976 ◽

Cited By ~ 1

Author(s):

Chun-Ting Lee ◽

Kristen L. Boeshore ◽

Chun Wu ◽

Kevin G. Becker ◽

Stacie L. Errico ◽

...

Keyword(s):

Astrocyte Proliferation ◽

Human Astrocyte ◽

Cyclin A2 ◽

Primary Human Astrocyte

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Modeling the effects of hyaluronic acid degradation on the regulation of human astrocyte phenotype using multicomponent interpenetrating polymer networks (mIPNs)

Scientific Reports ◽

10.1038/s41598-020-77655-1 ◽

2020 ◽

Vol 10 (1) ◽

Author(s):

Andrea C. Jimenez-Vergara ◽

Rachel Van Drunen ◽

Tyler Cagle ◽

Dany J. Munoz-Pinto

Keyword(s):

Hyaluronic Acid ◽

Calcium Binding ◽

Inflammatory Markers ◽

Polymer Networks ◽

Interpenetrating Polymer Networks ◽

Necrosis Factor Alpha ◽

Glycol Diacrylate ◽

Human Astrocyte ◽

Poly Ethylene ◽

Tissue Trauma

AbstractHyaluronic acid (HA) is a highly abundant component in the extracellular matrix (ECM) and a fundamental element to the architecture and the physiology of the central nervous system (CNS). Often, HA degradation occurs when an overreactive inflammatory response, derived from tissue trauma or neurodegenerative diseases such as Alzheimer’s, causes the ECM in the CNS to be remodeled. Herein, we studied the effects of HA content as a key regulator of human astrocyte (HAf) reactivity using multicomponent interpenetrating polymer networks (mIPNs) comprised of Collagen I, HA and poly(ethylene glycol) diacrylate. The selected platform facilities the modulation of HA levels independently of matrix rigidity. Total astrocytic processes length, number of endpoints, the expression of the quiescent markers: Aldehyde Dehydrogenase 1 Family Member L1 (ALDH1L1) and Glutamate Aspartate Transporter (GLAST); the reactive markers: Glial Fibrillary Acidic Protein (GFAP) and S100 Calcium-Binding Protein β (S100β); and the inflammatory markers: Inducible Nitric Oxide Synthase (iNOS), Interleukin 1β (IL-1β) and Tumor Necrosis Factor Alpha (TNFα), were assessed. Cumulatively, our results demonstrated that the decrease in HA concentration elicited a reduction in the total length of astrocytic processes and an increase in the expression of HAf reactive and inflammatory markers.

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