Decolonisation of MRSA, S. aureus and E. coli by Cold-Atmospheric Plasma Using a Porcine Skin Model In Vitro

Background: Over the past five years, the cold atmospheric plasma-activated solutions (PAS) have shown their promissing application in cancer treatment. Similar as the common direct cold plasma treatment, PAS shows a selective anti-cancer capacity in vitro and in vivo. However, different from the direct cold atmospheric plasma (CAP) treatment, PAS can be stored for a long time and can be used without dependence on a CAP device. The research on PAS is gradually becoming a hot topic in plasma medicine. Objectives: In this review, we gave a concise but comprehensive summary on key topics about PAS including the development, current status, as well as the main conclusions about the anti-cancer mechanism achieved in past years. The approaches to make strong and stable PAS are also summarized.

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The synergistic effect of Canady Helios cold atmospheric plasma and a FOLFIRINOX regimen for the treatment of cholangiocarcinoma in vitro

Scientific Reports ◽

10.1038/s41598-021-88451-w ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Olivia Jones ◽

Xiaoqian Cheng ◽

Saravana R. K. Murthy ◽

Lawan Ly ◽

Taisen Zhuang ◽

...

Keyword(s):

Cell Viability ◽

Chemotherapy Regimen ◽

Synergetic Effect ◽

Atmospheric Plasma ◽

High Recurrence Rate ◽

Cold Atmospheric Plasma ◽

Tract Cancer ◽

Anticancer Properties ◽

Potential Interactions

AbstractCholangiocarcinoma (CCA) is a rare biliary tract cancer with a low five-year survival rate and high recurrence rate after surgical resection. Currently treatment approaches include systemic chemotherapeutics such as FOLFIRINOX, a chemotherapy regimen is a possible treatment for severe CCA cases. A limitation of this chemotherapy regimen is its toxicity to patients and adverse events. There exists a need for therapies to alleviate the toxicity of a FOLFIRINOX regimen while enhancing or not altering its anticancer properties. Cold atmospheric plasma (CAP) is a technology with a promising future as a selective cancer treatment. It is critical to know the potential interactions between CAP and adjuvant chemotherapeutics. In this study the aim is to characterize the efficacy of FOLFIRINOX and CAP in combination to understand potential synergetic effect on CCA cells. FOLFIRINOX treatment alone at the highest dose tested (53.8 µM fluorouracil, 13.7 µM Leucovorin, 5.1 µM Irinotecan, and 3.7 µM Oxaliplatin) reduced CCA cell viability to below 20% while CAP treatment alone for 7 min reduced viability to 3% (p < 0.05). An analysis of cell viability, proliferation, and cell cycle demonstrated that CAP in combination with FOLFIRINOX is more effective than either treatment alone at a lower FOLFIRINOX dose of 6.7 µM fluorouracil, 1.7 µM leucovorin, 0.6 µM irinotecan, and 0.5 µM oxaliplatin and a shorter CAP treatment of 1, 3, or 5 min. In conclusion, CAP has the potential to reduce the toxicity burden of FOLFIRINOX and warrants further investigation as an adjuvant therapy.

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Cold atmospheric plasma coupled with air abrasion in liquid medium for the treatment of peri-implantitis model grown with a complex human biofilm: an in vitro study

Clinical Oral Investigations ◽

10.1007/s00784-021-03949-x ◽

2021 ◽

Author(s):

Wang Lai Hui ◽

Vittoria Perrotti ◽

Adriano Piattelli ◽

Kostya (Ken) Ostrikov ◽

Zhi Fang ◽

...

Keyword(s):

Liquid Medium ◽

Statistical Significance ◽

Atmospheric Plasma ◽

Air Abrasion ◽

Implant Surface ◽

Cold Atmospheric Plasma ◽

Surface Features ◽

Treatment Groups ◽

Surface Decontamination

Abstract Objective Treatment of implants with peri-implantitis is often unsuccessful due to residual microbial biofilm hindering re-osseointegration. The aim of this study was to treat biofilm-grown titanium (Ti) implants with different modalities involving air abrasion (AA) and cold atmospheric plasma (CAP) to compare the effectiveness in surface decontamination and the alteration/preservation of surface topography. Materials and methods Saliva collected from a peri-implantitis patient was used to in vitro develop human biofilm over 35 implants with moderately rough surface. The implants were then mounted onto standardized acrylic blocks simulating peri-implantitis defects and treated with AA (erythritol powder), CAP in a liquid medium, or a combination (COM) of both modalities. The remaining biofilm was measured by crystal violet (CV). Surface features and roughness before and after treatment were assessed by scanning electron microscope (SEM). The data were statistically analyzed using Kruskal-Wallis followed by Tukey’s multiple comparison test. Results In the present peri-implantitis model, the human complex biofilm growth was successful as indicated by the statistical significance between the negative and positive controls. All the treatment groups resulted in a remarkable implant surface decontamination, with values very close to the negative control for AA and COM. Indeed, statistically significant differences in the comparison between the positive control vs. all the treatment groups were found. SEM analysis showed no post-treatment alterations on the implant surface in all the groups. Conclusions Decontamination with AA delivering erythritol with or without CAP in liquid medium demonstrated compelling efficacy in the removal of biofilm from implants. All the tested treatments did not cause qualitative alterations to the Ti surface features. No specific effects of the CAP were observed, although further studies are necessary to assess its potential as monotherapy with different settings or in combination with other decontamination procedures. Clinical relevance CAP is a promising option in the treatment of peri-implantitis because it has potential to improve the elimination of bacterial plaque from implant surfaces, in inaccessible pockets or during open-flap debridement, and should stimulate the process of the re-osseointegration of affected dental implants by not altering surface features and roughness.

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The anti-cancer effect of cold atmospheric plasma in vitro

Cold Plasma Cancer Therapy ◽

10.1088/2053-2571/aafb9cch3 ◽

2019 ◽

Author(s):

Michael Keidar ◽

Dayun Yan ◽

Jonathan H Sherman

Keyword(s):

Atmospheric Plasma ◽

Cold Atmospheric Plasma ◽

Anti Cancer

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The efficacy and safety of cold atmospheric plasma as a novel therapy for diabetic wound in vitro and in vivo

International Wound Journal ◽

10.1111/iwj.13341 ◽

2020 ◽

Vol 17 (3) ◽

pp. 851-863 ◽

Cited By ~ 1

Author(s):

Rui He ◽

Qin Li ◽

Wenqi Shen ◽

Tao Wang ◽

Huijuan Lu ◽

...

Keyword(s):

Atmospheric Plasma ◽

Diabetic Wound ◽

Efficacy And Safety ◽

Novel Therapy ◽

Cold Atmospheric Plasma

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Comparative Study between Direct and Indirect Treatment with Cold Atmospheric Plasma on In Vitro and In Vivo Models of Wound Healing

Plasma Medicine ◽

10.1615/plasmamed.2019028659 ◽

2018 ◽

Vol 8 (4) ◽

pp. 379-401 ◽

Cited By ~ 1

Author(s):

Constance Duchesne ◽

Nadira Frescaline ◽

Jean-Jacques Lataillade ◽

Antoine Rousseau

Keyword(s):

Wound Healing ◽

Comparative Study ◽

Atmospheric Plasma ◽

In Vivo Models ◽

Cold Atmospheric Plasma ◽

Indirect Treatment

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The Emerging Role of Cold Atmospheric Plasma in Implantology: A Review of the Literature

Nanomaterials ◽

10.3390/nano10081505 ◽

2020 ◽

Vol 10 (8) ◽

pp. 1505 ◽

Cited By ~ 1

Author(s):

Wang Lai Hui ◽

Vittoria Perrotti ◽

Flavia Iaculli ◽

Adriano Piattelli ◽

Alessandro Quaranta

Keyword(s):

New Technologies ◽

Atmospheric Plasma ◽

Oral Diseases ◽

Air Plasma ◽

Review Of The Literature ◽

Air Abrasion ◽

Cold Atmospheric Plasma ◽

Osteoblast Activity

In recent years, cold atmospheric plasma (CAP) technologies have received increasing attention in the field of biomedical applications. The aim of this article is to review the currently available literature to provide an overview of the scientific principles of CAP application, its features, functions, and its applications in systemic and oral diseases, with a specific focus on its potential in implantology. In this narrative review, PubMed, Medline, and Scopus databases were searched using key words like “cold atmospheric plasma”, “argon plasma”, “helium plasma”, “air plasma”, “dental implants”, “implantology”, “peri-implantitis”, “decontamination”. In vitro studies demonstrated CAP’s potential to enhance surface colonization and osteoblast activity and to accelerate mineralization, as well as to determine a clean surface with cell growth comparable to the sterile control on both titanium and zirconia surfaces. The effect of CAP on biofilm removal was revealed in comparative studies to the currently available decontamination modalities (laser, air abrasion, and chlorhexidine). The combination of mechanical treatments and CAP resulted in synergistic antimicrobial effects and surface improvement, indicating that it may play a central role in surface “rejuvenation” and offer a novel approach for the treatment of peri-implantitis. It is noteworthy that the CAP conditioning of implant surfaces leads to an improvement in osseointegration in in vivo animal studies. To the best of our knowledge, this is the first review of the literature providing a summary of the current state of the art of this emerging field in implantology and it could represent a point of reference for basic researchers and clinicians interested in approaching and testing new technologies.

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Parameters Affecting the Antimicrobial Properties of Cold Atmospheric Plasma Jet

Journal of Clinical Medicine ◽

10.3390/jcm8111930 ◽

2019 ◽

Vol 8 (11) ◽

pp. 1930 ◽

Cited By ~ 7

Author(s):

Bih-Show Lou ◽

Chih-Ho Lai ◽

Teng-Ping Chu ◽

Jang-Hsing Hsieh ◽

Chun-Ming Chen ◽

...

Keyword(s):

Gas Flow ◽

Treatment Time ◽

Plasma Jet ◽

Atmospheric Plasma ◽

Gas Flow Rate ◽

Cold Atmospheric Plasma ◽

E Coli ◽

Antimicrobial Efficiency ◽

Ar Gas ◽

Sample Distance

Using the Taguchi method to narrow experimental parameters, the antimicrobial efficiency of a cold atmospheric plasma jet (CAPJ) treatment was investigated. An L9 array with four parameters of CAPJ treatments, including the application voltage, CAPJ-sample distance, argon (Ar) gas flow rate, and CAPJ treatment time, were applied to examine the antimicrobial activity against Escherichia coli (E. coli). CAPJ treatment time was found to be the most influential parameter in its antimicrobial ability by evaluation of signal to noise ratios and analysis of variance. 100% bactericidal activity was achieved under the optimal bactericidal activity parameters including the application voltage of 8.5 kV, CAPJ-sample distance of 10 mm, Ar gas flow rate of 500 sccm, and CAPJ treatment time of 300 s, which confirms the efficacy of the Taguchi method in this design. In terms of the mechanism of CAPJ’s antimicrobial ability, the intensity of hydroxyl radical produced by CAPJ positively correlated to its antimicrobial efficiency. The CAPJ antimicrobial efficiency was further evaluated by both DNA double-strand breaks analysis and scanning electron microscopy examination of CAPJ treated bacteria. CAPJ destroyed the cell wall of E. coli and further damaged its DNA structure, thus leading to successful killing of bacteria. This study suggests that optimal conditions of CPAJ can provide effective antimicrobial activity and may be grounds for a novel approach for eradicating bacterial infections.

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