17518 Background: Recently, some new factors, such as cytogenetic abnormalities, ZAP-70, proliferative antigen Ki-67, and the expression of CD38 in leukaemic cells, were strong indicator of prognosis in B-cell chronic lymphocytic leukaemia (B-CLL). The aim of this study was to explore the characteristics of molecular cytogenetics in Chinese patients with CLL, evaluate the expression of Ki-67, ZAP-70 and CD38 in leukaemic cells, and analyze the influence of factors on the prognosis of CLL. Methods: Interphase fluorescence in situ hybridization (FISH) was used to detect cytogenetic aberrations, and a panel of FISH probes for 13q14 (D13S319), 17p13 (p53 gene), 11q23 (ATM gene), the centromere of chromosome 12 (D12Z3) and 14q32 (IGHC/IGHV) was applied on bone marrow or peripheral blood smears from 52 Chinese B-CLL patients; Four-color flow cytometry was used to determined the expression of ZAP-70 protein and CD38; Immunohistochemistry was applied to measure the expression of Ki-67 antigen and Bcl-2 protein. Kaplan-Meier was used for survival time. Results: Out of the 52 patients, 42 (80.7%) had at least one kind of molecular cytogenetic aberration and 16 (30.8%) with 2 abnormalities. The most frequent abnormalities detected in our patients was deletions of 13q14 in 26 cases (50.0%), followed by trisomy of chromosome 12 in 11 patients (21.2%), deletions of 17p13 in 10 patients (19.2%), deletions of 11q23 in 6 patients (11.5%), and 14q32 translocation in 5 patients (9.6%). Fourteen patients (26.9%) were positive for ZAP-70 (=20%), and 13 patients (25.0%) were positive for CD38. Positive ZAP-70 and CD38 status was associated with advanced disease stage (Binet C). The expression levels of Ki-67 in Binet C stage was higher than that in early stage (Binet A and B). In univariate analysis for survival, 13q14 deletion was a favorable prognostic factor, and the deletions of 17p13 and 11q23 were poor prognostic factors. The survival time was longer in the group with lower expression of Ki-67 than that in the higher expression group. Both ZAP-70 and CD38 expression were shown to predict the clinical course of the disease. Conclusions: Chromosomal aberrations (deletions of 13q14, 17p13 and 11q23), and expression of Ki-67, ZAP-70 and CD38 have been shown highly predictive prognostic value for Chinese patients with B-CLL. No significant financial relationships to disclose.
Analyses of the Survival Time and the Influencing Factors of Chinese Patients with Prion Diseases Based on the Surveillance Data from 2008–2011
