Serum Amino Acids Profile and the Beneficial Effects of L-Arginine or L-Glutamine Supplementation in Dextran Sulfate Sodium Colitis

Colitis causes destruction of the intestinal mucus layer and increases intestinal inflammation. The use of antioxidants and anti-inflammatory agents derived from natural sources has been recently highlighted as a new approach for the treatment of colitis. Oxyresveratrol (OXY) is an antioxidant known to have various beneficial effects on human health, such as anti-inflammatory, antibacterial activity, and antiviral activity. The aim of this study was to investigate the therapeutic effect of OXY in rats with dextran sulfate sodium (DSS)-induced acute colitis. OXY ameliorated DSS-induced colitis and repaired damaged intestinal mucosa. OXY downregulated the expression of pro-inflammatory cytokine genes (TNF-α, IL-6, and IL-1β) and chemokine gene MCP-1, while promoting the production of anti-inflammatory cytokine IL-10. OXY treatment also suppressed inflammation via inhibiting cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS) expression in the colon, as well as the activity of myeloperoxidase (MPO). OXY exhibited anti-apoptotic effects, shifting the Bax/Bcl-2 balance. In conclusion, OXY might improve DSS-induced colitis by restoring the intestinal mucus layer and reducing inflammation within the intestine.

Download Full-text

Effects of dexfenfluramine on weight loss and serum amino acids profile in obese subjects

Nutrition Research ◽

10.1016/0271-5317(96)00186-8 ◽

1996 ◽

Vol 16 (10) ◽

pp. 1671-1678 ◽

Cited By ~ 1

Author(s):

Antonio S. del Barrio ◽

Alfredo Fernandez-Quintela ◽

Ana Rocandio ◽

Pedro M. Latorre ◽

Jose A. Vázquez

Keyword(s):

Amino Acids ◽

Weight Loss ◽

Serum Amino Acids ◽

Obese Subjects ◽

Amino Acids Profile

Download Full-text

Beneficial effects of eosinophils in colitis induced by dextran sulfate sodium*1

Journal of Allergy and Clinical Immunology ◽

10.1016/j.jaci.2004.01.053 ◽

2004 ◽

Vol 113 (2) ◽

pp. S172 ◽

Cited By ~ 1

Author(s):

T KOBAYASHI

Keyword(s):

Dextran Sulfate ◽

Dextran Sulfate Sodium ◽

Beneficial Effects

Download Full-text

Porcine circovirus type 2 affects the serum profile of amino acids and intestinal expression of amino acid transporters in mice

RSC Advances ◽

10.1039/c5ra12904d ◽

2015 ◽

Vol 5 (90) ◽

pp. 73651-73659 ◽

Cited By ~ 4

Author(s):

Dingfu Xiao ◽

Jie Yin ◽

Wenkai Ren ◽

Jianhua He ◽

Xionggui Hu ◽

...

Keyword(s):

Amino Acids ◽

Amino Acid ◽

Porcine Circovirus Type ◽

Porcine Circovirus Type 2 ◽

Porcine Circovirus ◽

Amino Acid Transporters ◽

Highly Pathogenic ◽

Serum Amino Acids ◽

Amino Acids Profile

PCV2 is highly pathogenic, however, its effect on the serum amino acids profile is unknown.

Download Full-text

Anti-Inflammatory and Gut Microbiota Modulatory Effect of Lactobacillus rhamnosus Strain LDTM 7511 in a Dextran Sulfate Sodium-Induced Colitis Murine Model

Microorganisms ◽

10.3390/microorganisms8060845 ◽

2020 ◽

Vol 8 (6) ◽

pp. 845

Author(s):

Soyoung Yeo ◽

Hyunjoon Park ◽

Eunsol Seo ◽

Jihee Kim ◽

Byoung Kook Kim ◽

...

Keyword(s):

Gut Microbiota ◽

Dextran Sulfate ◽

Dextran Sulfate Sodium ◽

Lactobacillus Rhamnosus ◽

Probiotic Strain ◽

Experimental Models ◽

Unknown Etiology ◽

Opposite Pattern ◽

Beneficial Effects ◽

Probiotic Treatment

Inflammatory bowel disease (IBD) is a group of conditions involving chronic relapsing-remitting inflammation of the gastrointestinal tract with an unknown etiology. Although the cause–effect relationship between gut microbiota and IBD has not been clearly established, emerging evidence from experimental models supports the idea that gut microbes play a fundamental role in the pathogenesis of IBD. As microbiome-based therapeutics for IBD, the beneficial effects of probiotics have been found in animal colitis models and IBD patients. In this study, based on the dextran sulfate sodium (DSS)-induced colitis mouse model, we investigated Lactobacillus rhamnosus strain LDTM 7511 originating from Korean infant feces as a putative probiotic strain for IBD. The strain LDTM 7511 not only alleviated the release of inflammatory mediators, but also induced the transition of gut microbiota from dysbiotic conditions, exhibiting the opposite pattern in the abundance of DSS colitis-associated bacterial taxa to the DSS group. Our findings suggest that the strain LDTM 7511 has the potential to be used as a probiotic treatment for IBD patients in comparison to L. rhamnosus GG (ATCC 53103), which has been frequently used for IBD studies.

Download Full-text

Assessment of serum amino acids profile in adult patients with Crohn’s disease

Clinical Nutrition ESPEN ◽

10.1016/j.clnesp.2021.09.254 ◽

2021 ◽

Vol 46 ◽

pp. S632

Author(s):

I. Cioffi ◽

M. Fisco ◽

L. Santarpia ◽

O. Di Vincenzo ◽

M. Caterino ◽

...

Keyword(s):

Amino Acids ◽

Crohn’S Disease ◽

Crohn's Disease ◽

Adult Patients ◽

Serum Amino Acids ◽

Amino Acids Profile

Download Full-text

Beneficial effects of the traditional medicine Igongsan and its constituent ergosterol on dextran sulfate sodium-induced colitis in mice

Molecular Medicine Reports ◽

10.3892/mmr.2015.3824 ◽

2015 ◽

Vol 12 (3) ◽

pp. 3549-3556 ◽

Cited By ~ 3

Author(s):

SU-JIN KIM ◽

HYUN-JI SHIN ◽

GEUN-HYUK LEE ◽

DAE-SEUNG KIM ◽

HYE-LIN KIM ◽

...

Keyword(s):

Traditional Medicine ◽

Dextran Sulfate ◽

Dextran Sulfate Sodium ◽

Beneficial Effects

Download Full-text

Bisphenol-A alters microbiota metabolites derived from aromatic amino acids and worsens disease activity during colitis

Experimental Biology and Medicine ◽

10.1177/1535370218782139 ◽

2018 ◽

Vol 243 (10) ◽

pp. 864-875 ◽

Cited By ~ 12

Author(s):

Jennifer AA DeLuca ◽

Kimberly F Allred ◽

Rani Menon ◽

Rebekah Riordan ◽

Brad R Weeks ◽

...

Keyword(s):

Amino Acids ◽

Inflammatory Bowel Disease ◽

Bisphenol A ◽

Disease Activity ◽

Bowel Disease ◽

Dextran Sulfate ◽

Dextran Sulfate Sodium ◽

Aromatic Amino Acids ◽

Colonic Inflammation ◽

Inflammatory Bowel

Inflammatory bowel disease is a complex collection of disorders. Microbial dysbiosis as well as exposure to toxins including xenoestrogens are thought to be risk factors for inflammatory bowel disease development and relapse. Bisphenol-A has been shown to exert estrogenic activity in the colon and alter intestinal function, but the role that xenoestrogens, such as bisphenol-A , play in colonic inflammation has been previously described but with conflicting results. We investigated the ability of bisphenol-A to exacerbate colonic inflammation and alter microbiota metabolites derived from aromatic amino acids in an acute dextran sulfate sodium-induced colitis model. Female C57BL/6 mice were ovariectomized and exposed to bisphenol-A daily for 15 days. Disease activity measures include body weight, fecal consistency, and rectal bleeding. Colons were scored for inflammation, injury, and nodularity. Alterations in the levels of microbiota metabolites derived from aromatic amino acids known to reflect phenotypic changes in the gut microbiome were analyzed. Bisphenol-A exposure increased mortality and worsened disease activity as well as inflammation and nodularity scores in the middle colon region following dextran sulfate sodium exposure. Unique patterns of metabolites were associated with bisphenol-A consumption. Regardless of dextran sulfate sodium treatment, bisphenol-A reduced levels of tryptophan and several metabolites associated with decreased inflammation in the colon. This is the first study to show that bisphenol-A treatment alone can reduce microbiota metabolites derived from aromatic amino acids in the colon which may be associated with increased colonic inflammation and inflammatory bowel disease. Impact statement As rates of inflammatory bowel disease rise, discovery of the mechanisms related to the development of these conditions is important. Environmental exposure is hypothesized to play a role in etiology of the disease, as are alterations in the gut microbiome and the metabolites they produce. This study is the first to show that bisphenol-A alone alters tryptophan and microbiota metabolites derived from aromatic amino acids in a manner consistent with autoimmune diseases, specifically inflammatory bowel diseases, regardless of dextran sulfate sodium treatment. These findings indicate a potential mechanism by which bisphenol-A negatively affects gut physiology to exacerbate inflammation.

Download Full-text

Protective Effects of Shrimp Peptide on Dextran Sulfate Sodium-Induced Colitis in Mice

Frontiers in Nutrition ◽

10.3389/fnut.2021.773064 ◽

2021 ◽

Vol 8 ◽

Author(s):

Xingwei Xiang ◽

Qihong Jiang ◽

Wan Shao ◽

Jinhong Li ◽

Yufang Zhou ◽

...

Keyword(s):

Amino Acids ◽

Dextran Sulfate ◽

Dextran Sulfate Sodium ◽

Intestinal Flora ◽

Intestinal Barrier ◽

16S Rrna Genes ◽

Rrna Genes ◽

Protective Effects ◽

Mice Model

Inflammatory bowel disease, an intestinal relapsing inflammatory disease, not only impairs gastrointestinal function but also increases the chances of developing colon cancer. Currently, the effects of shrimp peptide (SP) in mice model of ulcerative colitis (UC) are still unclear. In particular, it is uncertain whether SP affects the gut flora with UC mice. In this study, we investigated the anti-inflammatory effects of SP on a dextran sulfate sodium (DSS)-induced mouse model of UC. Firstly, the molecular weight of SP was mainly distributed in the range of 180–1,000 Da (61.95% proportion), and the amino acid composition showed that SP contained 17 amino acids, of which, the essential amino acids accounted for 54.50%. In vivo, oral SP significantly attenuated the severity of colitis, such as diarrhea, weight loss, and rectal bleeding. Furthermore, treatment with SP remarkably ameliorated intestinal barrier integrity, thus lowering the levels of the inflammatory cytokines and ameliorating antioxidant indices and intestinal injury indicators in the serum and colon. Lastly, the cecal contents were used to sequence and analyze the 16S rRNA genes of bacteria. Results suggested that treatment with SP could restore the balance of intestinal flora in modeled mice by regulating the abundance of pathogenic and beneficial bacteria. Furthermore, SP could significantly improve intestinal flora dysfunction in mice with UC. In summary, our findings show that SP has a prophylactic and therapeutic effect in UC in vivo, thereby highlighting its broad medicinal applications.

Download Full-text