Effect of Larval Competition on Extrinsic Incubation Period and Vectorial Capacity of Aedes albopictus for Dengue Virus

We report a study on vector competence of an Italian population of Aedes albopictus for Zika virus (ZIKV). Ae. albopictus was susceptible to ZIKV infection (infection rate: 10%), and the virus could disseminate and was secreted in the mosquito’s saliva (dissemination rate: 29%; transmission rate: 29%) after an extrinsic incubation period of 11 days. The observed vector competence was lower than that of an Ae. aegypti colony tested in parallel.

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Transcriptome Analysis of Responses to Dengue Virus 2 Infection in Aedes albopictus (Skuse) C6/36 Cells

Viruses ◽

10.3390/v13020343 ◽

2021 ◽

Vol 13 (2) ◽

pp. 343

Author(s):

Manjin Li ◽

Dan Xing ◽

Duo Su ◽

Di Wang ◽

Heting Gao ◽

...

Keyword(s):

Dengue Virus ◽

Aedes Albopictus ◽

Bioinformatics Analysis ◽

Interaction Mechanism ◽

Transcriptional Analysis ◽

Functional Verification ◽

Mosquito Vector ◽

Rna Seq ◽

Sequencing Data ◽

Qrt Pcr

Dengue virus (DENV), a member of the Flavivirus genus of the Flaviviridae family, can cause dengue fever (DF) and more serious diseases and thus imposes a heavy burden worldwide. As the main vector of DENV, mosquitoes are a serious hazard. After infection, they induce a complex host–pathogen interaction mechanism. Our goal is to further study the interaction mechanism of viruses in homologous, sensitive, and repeatable C6/36 cell vectors. Transcriptome sequencing (RNA-Seq) technology was applied to the host transcript profiles of C6/36 cells infected with DENV2. Then, bioinformatics analysis was used to identify significant differentially expressed genes and the associated biological processes. Quantitative reverse transcription-polymerase chain reaction (qRT-PCR) was performed to verify the sequencing data. A total of 1239 DEGs were found by transcriptional analysis of Aedes albopictus C6/36 cells that were infected and uninfected with dengue virus, among which 1133 were upregulated and 106 were downregulated. Further bioinformatics analysis showed that the upregulated DEGs were significantly enriched in signaling pathways such as the MAPK, Hippo, FoxO, Wnt, mTOR, and Notch; metabolic pathways and cellular physiological processes such as autophagy, endocytosis, and apoptosis. Downregulated DEGs were mainly enriched in DNA replication, pyrimidine metabolism, and repair pathways, including BER, NER, and MMR. The qRT-PCR results showed that the concordance between the RNA-Seq and RT-qPCR data was very high (92.3%). The results of this study provide more information about DENV2 infection of C6/36 cells at the transcriptome level, laying a foundation for further research on mosquito vector–virus interactions. These data provide candidate antiviral genes that can be used for further functional verification in the future.

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Entomological Status Based on Vector Density Index and Transovarial Infection on Aedes Sp. Mosquito in Meteseh Village, Semarang City

E3S Web of Conferences ◽

10.1051/e3sconf/202020212016 ◽

2020 ◽

Vol 202 ◽

pp. 12016

Author(s):

Martini Martini ◽

Zufri Armen ◽

Kusariana Nissa ◽

Hestiningsih Retno ◽

Yuliawati Sri ◽

...

Keyword(s):

Health Services ◽

Dengue Virus ◽

Aedes Albopictus ◽

Research Method ◽

Hemorrhagic Fever ◽

Adult Mosquito ◽

Elisa Method ◽

Density Index ◽

Egg Density ◽

Descriptive Method

Dengue Hemorrhagic Fever (DHF) is a disease caused by Dengue virus. In 2016, the number of DHF patients in areas of Health services of Rowosari was 247 cases, IR 289.6/100.000 populations and the highest number of the patients was from Meteseh Village. The most usual mechanism happened in the vertical transmission, transovarial infection, from adult mosquito to the egg. The study was conducted to describe of entomological status of House Index (HI), Container Index (CI), Breteau Index (BI), House index (HI), Ovitrap Index (OI) of larvae as well as transovarial infection in Aedes sp. The research method was descriptive method by conducting test to the entomology status of DHF incidence. Based on the survey in Meteseh sub-village (XVI), a result found that average House Index 8.99%, Container Index 2.88%, Breteau Index 16.40%, HI 8,99 Ovitrap Index 35.46% with the egg density at 1.023 eggs. The identified mosquitoes were 30, and from the identification result of Aedes sp., two Aedes species were found, 28 Aedes aegypti,and only 2 Aedes albopictus. The transovarial infection in Aedes sp. using ELISA method was found 6 mosquito samples which were positive of dengue virus, with the detail in neighborhood 01 (1 sample), neighborhood 02 (2 samples), neighborhood 03 (3 samples). DHF in Meteseh Sub village in Semarang City was probably happened because correlated with transovarial infection.

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Vector competence of Malaysian Aedes albopictus with and without Wolbachia to four dengue virus serotypes

Tropical Medicine & International Health ◽

10.1111/tmi.12918 ◽

2017 ◽

Vol 22 (9) ◽

pp. 1154-1165 ◽

Cited By ~ 9

Author(s):

Sylvia Joanne ◽

Indra Vythilingam ◽

Boon-Teong Teoh ◽

Cherng-Shii Leong ◽

Kim-Kee Tan ◽

...

Keyword(s):

Dengue Virus ◽

Aedes Albopictus ◽

Vector Competence ◽

Dengue Virus Serotypes

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Vector survival and parasite infection: the effect of Wuchereria bancrofti on its vector Culex quinquefasciatus

Parasitology ◽

10.1017/s0031182004005153 ◽

2004 ◽

Vol 129 (1) ◽

pp. 43-50 ◽

Cited By ~ 11

Author(s):

K. KRISHNAMOORTHY ◽

S. SUBRAMANIAN ◽

G. J. VAN OORTMARSSEN ◽

J. D. F. HABBEMA ◽

P. K. DAS

Keyword(s):

Incubation Period ◽

Culex Quinquefasciatus ◽

Geometric Mean ◽

Wuchereria Bancrofti ◽

Parasite Load ◽

Infected Mosquito ◽

Parasite Development ◽

Mosquito Vector ◽

Extrinsic Incubation Period ◽

Parasite Loss

This paper investigates a cohort of 2187 laboratory reared Culex quinquefasciatus fed on 69 human volunteers, including 59 persons with different levels of Wuchereria bancrofti microfilariae and 10 without microfilaria. Mosquitoes were followed until death. Mosquito survival was analysed in relation to the level of microfilaria in the human and larval count in the dead mosquito. Vector mortality during the extrinsic incubation period (12 days post-engorgement) was significantly higher in mosquitoes fed on microfilaraemic volunteers (50%) than in those fed on amicrofilaraemics (29%). Both the percentage infected and the geometric mean parasite density was significantly higher among mosquitoes which died before 13 days (45% infected and 10 larvae per infected mosquito) than those surviving beyond 13 days (39% and 2·2), suggesting a parasite loss of more than 80% during the extrinsic incubation period. A large proportion (62%) of the mosquitoes that died during the early of phase of parasite development were infected (36% in low, 26% in medium and 90% in high human Mf-density). Survival analysis showed that the parasite load in mosquitoes and the human Mf-density for a given parasite load are independent risk factors of vector survival. Overall, the hazard of dying was found to be 11–15 times higher among mosquitoes fed on microfilaraemic volunteers than those fed on amicrofilaraemics. The hazard doubles for every increase of about 60–70 parasites in the vector. As a consequence of the parasite-induced reduction in vector survival, the transmission success of the parasite is reduced. The implication of the results on control/elimination of lymphatic filariasis using mass-drug administration is discussed.

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