scholarly journals The use of embryonic chicken eggs as an alternative model to evaluate the virulence of Salmonella enterica serovar Gallinarum

PLoS ONE ◽  
2020 ◽  
Vol 15 (9) ◽  
pp. e0238630
Author(s):  
Jun-feng Zhang ◽  
Bai Wei ◽  
Se-Yeoun Cha ◽  
Ke Shang ◽  
Hyung-Kwan Jang ◽  
...  
Keyword(s):  
Salmonella Enterica ◽  
Alternative Model ◽  
Embryonic Chicken ◽  
Chicken Eggs

scholarly journals Characterization of Antibiotic Resistant Salmonella spp Isolated from Chicken Eggs of Dhaka City

2010 ◽  
Vol 3 (1) ◽  
pp. 191 ◽  
Author(s):  
M. M. Ahmed ◽  
M. M. Rahman ◽  
K. R. Mahbub ◽  
M. Wahiduzzaman
Keyword(s):  
Antibiotic Resistance ◽  
Salmonella Enterica ◽  
Egg Yolk ◽  
Dhaka City ◽  
Salmonella Spp ◽  
Antibiotic Resistant ◽  
Poultry Eggs ◽  
Potential Reservoir ◽  
Egg Shell ◽  
Chicken Eggs

In present study, the occurrence of Salmonella in local chicken eggs and their pattern of antibiotic resistance were determined. 100 egg samples collected from different locations of Dhaka city were analysed and Salmonella spp were found in 8% of the samples. Among all presumptive Salmonella isolates, 8 isolates were confirmed as Salmonella enterica subsp. salamae (4%), Salmonella enterica subsp. indica (1%), Salmonella Paratyphi-A (1%), Salmonella bongori (1%) and Salmonella Choleraesuis (1%) on the basis of serotyping and biochemical analysis. These isolates were subjected to susceptibility test against 10 antibiotic disks. All the isolates were found chloramphenicol sensitive. The highest percentage of resistance (87.5%) was found to amoxicillin and ampicillin. Resistance against erythromycin, cephalexin, doxycycline hydrochloride, ceftazidime, doxycycline and nalidixic acid was also found significant ranging from 25% to 62.5%. Salmonella isolated from egg shell surface were found more antibiotic resistant than that of egg yolk and white. The present study suggests that poultry eggs are potential reservoir of antibiotic resistant Salmonellae.Keywords: Egg; Salmonella; Characterization; Antibiotic resistance.© 2011 JSR Publications. ISSN: 2070-0237 (Print); 2070-0245 (Online). All rights reserved.doi:10.3329/jsr.v3i1.6109                J. Sci. Res. 3 (1), 191-196  (2011)

scholarly journals Determination of the Optimum Time for Preparation of Half-Boiled Eggs Free from Salmonella Enterica Enteritidis

2018 ◽  
Vol 3 (1) ◽  
pp. 16
Author(s):  
Mohamad Fithri Akmar Omar ◽  
Nurdiyana Syahirah Abdul Manaf ◽  
Siti Nurnajwa Nadhirah Mohd Sufian ◽  
Norsuhaida Zakaria ◽  
Nur Farhah Najwa Ayub ◽  
...  
Keyword(s):  
Salmonella Enterica ◽  
Hot Water ◽  
Average Weight ◽  
Salmonella Enterica Serovar Enteritidis ◽  
Optimum Time ◽  
Colony Forming Units ◽  
Time Required ◽  
Chicken Eggs ◽  
Boiled Water

Introduction: Consuming half-boiled eggs during breakfast is popular among Malaysians. The eggs are usually prepared by submerging them in freshly boiled water for about 20 minutes duration. As chickens have the likelihood of harbouring Salmonella enterica Serovar Enteritidis in their eggs, it is feared that consumption of half-boiled eggs contaminated with S. Enteritidis could lead to gastroenteritis. Hence, this study was carried out to determine the optimum time required for boiled water to kill S. Enteritidis in half-boiled eggs. Methods: Grade C chicken eggs (average weight 54.5 g) were obtained from a local sundry shop and were confirmed to be Salmonella-free prior to the experiment. Fifteen eggs that were previously spiked with 106 colony-forming units (CFU) of S. Enteritidis were completely submerged into freshly-boiled water (≈100ºC) for a maximum of 20 min. Positive controls consisted of eggs that were inoculated but were not treated. At every 5 min interval, three eggs were taken out and checked for presence of viable S. Enteritidis on MacConkey agar, and the respective water temperature was recorded. Results: Results of two independent trials showed that after 10 min of submerging the eggs in the hot water, there were an average of 3 log reductions in the CFU of S. Enteritidis but after 15 min, no viable S. Enteritidis was detected. Conclusion: A half-boiled egg should be prepared by submerging it in freshly boiled water for at least 15 min to ensure that it is safe to be consumed.

Emergence of Salmonella enterica Serovar Potsdam as a Major Serovar in Waterfowl Hatcheries and Chicken Eggs

2011 ◽  
Vol 6 (2) ◽  
pp. e37-e38
Author(s):  
Yao-Chi Su ◽  
Chang-You Yu ◽  
Jiang-Liang Lin ◽  
Jyh-Mirn Lai ◽  
Shu-Wun Chen ◽  
...  
Keyword(s):  
Salmonella Enterica ◽  
Chicken Eggs

scholarly journals Detection of Salmonella Enterica in Egg Yolk by PCR on a Microfluidic Disc Device Using Immunomagnetic Beads

Sensors ◽  
2020 ◽  
Vol 20 (4) ◽  
pp. 1060 ◽  
Author(s):  
Izumi Kubo ◽  
Mitsutoshi Kajiya ◽  
Narumi Aramaki ◽  
Shunsuke Furutani
Keyword(s):  
Salmonella Enterica ◽  
Magnetic Beads ◽  
Egg Yolk ◽  
Sampling Time ◽  
Foodborne Illness ◽  
Immunomagnetic Beads ◽  
Inva Gene ◽  
Viable Cells ◽  
Chicken Eggs ◽  
Pcr Targeting

Salmonella enterica is a pathogenic bacterium that causes foodborne illness. One of the vehicle foods of S. enterica are chicken eggs. Efficient collection of the bacterium is necessary to detect it specifically. We developed a method to detect S. enterica by PCR on a microfluidic disc device using a fluorescent probe. Salmonella enterica cells were isolated in the microchambers on the device, followed by thermal lysis and PCR targeting with the invA gene, a gene specific to S. enterica, were observed by measurement of the fluorescent signal that resulted from gene amplification. However, the developed method was unable to discriminate viable cells from dead cells. Consequently, in this study, magnetic beads modified with anti-Salmonella antibody were utilized to detect viable Salmonella cells from egg yolk prior to PCR on the device. While using the antibody-modified beads, egg yolk components, which inhibit PCR, were removed. The collected cells were subsequently detected by PCR of the invA gene on a microfluidic disc device. This method enabled the detection of viable cells without the inhibition of PCR by any egg component. S. enterica was detected at 5.0×104 cells mL−1 or at a higher concentration of egg yolk within 6 h including the sampling time.

scholarly journals Identification of Genes Associated with Survival of Salmonella enterica Serovar Enteritidis in Chicken Egg Albumen

2006 ◽  
Vol 72 (2) ◽  
pp. 1055-1064 ◽  
Author(s):  
Raul I. Clavijo ◽  
Cindy Loui ◽  
Gary L. Andersen ◽  
Lee W. Riley ◽  
Sangwei Lu
Keyword(s):  
Salmonella Enterica ◽  
Human Population ◽  
Acid Metabolism ◽  
Clinical Manifestations ◽  
Salmonella Enterica Serovar Enteritidis ◽  
Serovar Typhimurium ◽  
Contaminated Food ◽  
Egg Albumen ◽  
Chicken Eggs ◽  
Epidemiological Characteristics

ABSTRACT Salmonella enterica consists of over 2,000 serovars that are major causes of morbidity and mortality associated with contaminated food. Despite similarities among serovars of Salmonella enterica, many demonstrate unique host specificities, epidemiological characteristics, and clinical manifestations. One of the unique epidemiological characteristics of the serovar Enteritidis is that it is the only bacterium routinely transmitted to humans through intact chicken eggs. Therefore, Salmonella enterica serovar Enteritidis must be able to persist inside chicken eggs to be transmitted to humans, and its survival in egg is important for its transmission to the human population. The ability of Salmonella enterica serovar Enteritidis to survive in and transmit through eggs may have contributed to its drastically increased prevalence in the 1980s and 1990s. In the present study, using transposon-mediated mutagenesis, we have identified genes important for the association of Salmonella enterica serovar Enteritidis with chicken eggs. Our results indicate that genes involved in cell wall structural and functional integrity, and nucleic acid and amino acid metabolism are important for Salmonella enterica serovar Enteritidis to persist in egg albumen. Two regions unique to Salmonella enterica serovar Enteritidis were also identified, one of which enhanced the survival of a Salmonella enterica serovar Typhimurium isolate in egg albumen. The implication of our results to the serovar specificity of Salmonella enterica is also explored in the present study.

Emergence of Salmonella enterica Serovar Potsdam as a Major Serovar in Waterfowl Hatcheries and Chicken Eggs

2011 ◽  
Vol 55 (2) ◽  
pp. 217-222 ◽  
Author(s):  
Yao-Chi Su ◽  
Chang-You Yu ◽  
Jiang-Liang Lin ◽  
Jyh-Mirn Lai ◽  
Shu-Wun Chen ◽  
...  
Keyword(s):  
Salmonella Enterica ◽  
Chicken Eggs

Embryonated chicken eggs as an alternative model for mixed Clostridium perfringens and Eimeria tenella infection in chickens

2013 ◽  
Vol 112 (6) ◽  
pp. 2299-2306 ◽  
Author(s):  
Alaa Aldin Alnassan ◽  
Awad Ali Shehata ◽  
Marianne Kotsch ◽  
Matthias Lendner ◽  
Arwid Daugschies ◽  
...  
Keyword(s):  
Clostridium Perfringens ◽  
Alternative Model ◽  
Eimeria Tenella ◽  
Chicken Eggs ◽  
Embryonated Chicken

An Electron Microscopic Study of an Avian Reovirus that Causes Arthritis

1971 ◽  
Vol 29 ◽  
pp. 254-255
Author(s):  
E, R. Walker ◽  
N. O. Olson ◽  
M. H. Friedman
Keyword(s):  
Electron Microscopy ◽  
Viral Genome ◽  
Inner Core ◽  
Avian Reovirus ◽  
Electron Microscopic ◽  
Outer Coat ◽  
Acid Type ◽  
Chicken Eggs ◽  
Icosahedral Particle ◽  
Mature Virus

An unidentified virus, responsible for an arthritic-like condition in chickens was studied by electron microscopy and other methods of viral investigation. It was characterized in chorio-allantoic membrane (CAM) lesions of embryonating chicken eggs and in tissue culture as to: 1) particle size; 2) structure; 3) mode of replication in the cell; and 4) nucleic acid type.The inoculated virus, coated and uncoated, is first seen in lysosomal-like inclusions near the nucleus; the virions appear to be uncoated in these electron dense inclusions (Figure 1), Although transfer of the viral genome from these inclusions is not observable, replicating virus and mature virus crystals are seen in the cytoplasm subsequent to the uncoating of the virions.The crystals are formed in association with a mass of fibrils 50 to 80 angstroms in diameter and a ribosome-studded structure that appears to be granular endoplasmic reticulum adapted to virus replication (Figure 2). The mature virion (Figure 3) is an icosahedral particle approximately 75 millimicrons in diameter. The inner core is 45 millimicrons, the outer coat 15 millimicrons, and the virion has no envelope.

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