scholarly journals Fungus-originated glucanase and monooxygenase genes in creeping bent grass (Agrostis stolonifera L.)

PLoS ONE ◽  
2021 ◽  
Vol 16 (9) ◽  
pp. e0257173
Author(s):  
Yugo Watanabe ◽  
German C. Spangenberg ◽  
Hiroshi Shinozuka
Keyword(s):  
Phylogenetic Analysis ◽  
Gene Transfer ◽  
Close Relationships ◽  
Fungal Endophyte ◽  
Agrostis Stolonifera ◽  
Plant Tissues ◽  
Cool Season ◽  
Ancestral Gene ◽  
Current Article ◽  
Clade 1

Recent studies have revealed presence of fungus-originated genes in genomes of cool-season grasses, suggesting occurrence of multiple ancestral gene transfer events between the two distant lineages. The current article describes identification of glucanase-like and monooxygenase-like genes from creeping bent grass, as lateral gene transfer candidates. An in silico analysis suggested presence of the glucanase-like gene in Agrostis, Deyeuxia, and Polypogon genera, but not in other species belonging to the clade 1 of the Poeae tribe. Similarly, the monooxygenase-like gene was confined to Agrostis and Deyeuxia genera. A consistent result was obtained from PCR-based screening. The glucanase-like gene was revealed to be ubiquitously expressed in young seedlings of creeping bent grass. Although expression of the monooxygenase-like gene was suggested in plant tissues, the levels were considerably lower than those of the glucanase-like gene. A phylogenetic analysis revealed close relationships of the two genes between the corresponding genes in fungal endophyte species of the Epichloë genus, suggesting that the genes originated from the Epichloë lineage.

scholarly journals Fungus-originated genes in the genomes of cereal and pasture grasses acquired through ancient lateral transfer

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Hiroshi Shinozuka ◽  
Maiko Shinozuka ◽  
Ellen M. de Vries ◽  
Timothy I. Sawbridge ◽  
German C. Spangenberg ◽  
...  
Keyword(s):  
Gene Transfer ◽  
Dna Sequence ◽  
Sequence Similarity ◽  
Fungal Endophyte ◽  
Grass Species ◽  
Cool Season ◽  
Forage Crops ◽  
Ancestral Gene ◽  
Pasture Grasses ◽  
Sequence Similarity Analysis

AbstractEvidence for ancestral gene transfer between Epichloë fungal endophyte ancestors and their host grass species is described. From genomes of cool-season grasses (the Poeae tribe), two Epichloë-originated genes were identified through DNA sequence similarity analysis. The two genes showed 96% and 85% DNA sequence identities between the corresponding Epichloë genes. One of the genes was specific to the Loliinae sub-tribe. The other gene was more widely conserved in the Poeae and Triticeae tribes, including wheat (Triticum aestivum L.) and barley (Hordeum vulgare L.). The genes were independently transferred during the last 39 million years. The transferred genes were expressed in plant tissues, presumably retaining molecular functions. Multiple gene transfer events between the specific plant and fungal lineages are unique. A range of cereal crops is included in the Poeae and Triticeae tribes, and the Loliinae sub-tribe is consisted of economically important pasture and forage crops. Identification and characterisation of the 'natural' adaptation transgenes in the genomes of cereals, and pasture and forage grasses, that worldwide underpin the production of major foods, such as bread, meat, and milk, may change the ‘unnatural’ perception status of transgenic and gene-edited plants.

Use of tall fescue EST-SSR markers in phylogenetic analysis of cool-season forage grasses

Genome ◽  
2005 ◽  
Vol 48 (4) ◽  
pp. 637-647 ◽  
Author(s):  
M A. Rouf Mian ◽  
Malay C Saha ◽  
Andrew A Hopkins ◽  
Zeng-Yu Wang
Keyword(s):  
Phylogenetic Analysis ◽  
Ssr Markers ◽  
Tall Fescue ◽  
Related Species ◽  
Grass Species ◽  
Forage Grass ◽  
Forage Grasses ◽  
Cool Season ◽  
Phylogenetic Relations ◽  
Wide Range

Microsatellites or simple sequence repeats (SSRs) are highly useful molecular markers for plant improvement. Expressed sequence tag (EST)-SSR markers have a higher rate of transferability across species than genomic SSR markers and are thus well suited for application in cross-species phylogenetic studies. Our objectives were to examine the amplification of tall fescue EST-SSR markers in 12 grass species representing 8 genera of 4 tribes from 2 subfamilies of Poaceae and the applicability of these markers for phylogenetic analysis of grass species. About 43% of the 145 EST-SSR primer pairs produced PCR bands in all 12 grass species and had high levels of polymorphism in all forage grasses studied. Thus, these markers will be useful in a variety of forage grass species, including the ones tested in this study. SSR marker data were useful in grouping genotypes within each species. Lolium temulentum, a potential model species for cool-season forage grasses, showed a close relation with the major Festuca–Lolium species in the study. Tall wheatgrass was found to be closely related to hexaploid wheat, thereby confirming the known taxonomic relations between these species. While clustering of closely related species was found, the effectiveness of such data in evaluating distantly related species needs further investigations. The phylogenetic trees based on DNA sequences of selected SSR bands were in agreement with the phylogenetic relations based on length polymorphism of SSRs markers. Tall fescue EST-SSR markers depicted phylogenetic relations among a wide range of cool-season forage grass species and thus are an important resource for researchers working with such grass species.Key words: phylogeny, EST-SSR, forage grasses, tall fescue.

scholarly journals Complete Genome Sequence of a Little Cherry Virus-2 Isolate from Sweet Cherry in China

2019 ◽  
Vol 8 (37) ◽  
Author(s):  
Dongzi Zhu ◽  
Jiawei Wang ◽  
Xiaojuan Zong ◽  
Yue Tan ◽  
Hairong Wei ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Genome Sequence ◽  
Small Rna ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Close Relationships ◽  
Sweet Cherry ◽  
Rt Pcr ◽  
Rna Deep Sequencing ◽  
Small Rna Deep Sequencing

The first complete genome sequence of a little cherry virus-2 (LChV-2-TA) isolate from China was determined using small RNA deep sequencing combined with overlapping reverse transcriptase PCR (RT-PCR). Phylogenetic analysis revealed that LChV-2-TA grouped in a well-supported cluster with members of the genus Ampelovirus with close relationships to previously reported LChV-2 isolates.

A note on the presence of phasmids on the male tails of Anguillulina multicincta, A. erythrinae and A. robusta

1948 ◽  
Vol 22 (3-4) ◽  
pp. 139-140 ◽  
Author(s):  
T. Goodey
Keyword(s):  
Lolium Perenne ◽  
Agrostis Stolonifera ◽  
Plant Roots ◽  
Plant Tissues ◽  
Grass Roots ◽  
De Man

In an earlier paper Goodey (1940) gave a detailed description of the morphology of three species of Anguillulina viz.: A. multicincta (Cobb) Goodey, A. erythrinae (Zimmermann) Goodey and A. robusta (de Man) Goodey. All three species are associated with plant roots: A. multicincta is a parasite of banana roots, A. erythrinae is parasitic on the roots of certain common grasses and cereals such as Agrostis stolonifera, Lolium perenne and oats (the writer has also found it on hop roots), A. robusta though associated with grass roots has not, to the writer's knowledge, been found within plant tissues. In the case of all three species phasmids were easily recognized on the female tails and were described and figured but they were not seen on the male tails and it was assumed that they were probably absent.

Absorption, Translocation, and Metabolism of Amicarbazone in Annual Bluegrass (Poa annua), Creeping Bentgrass (Agrostis stolonifera), and Tall Fescue (Festuca arundinacea)

Weed Science ◽  
2013 ◽  
Vol 61 (2) ◽  
pp. 217-221 ◽  
Author(s):  
Jialin Yu ◽  
Patrick E. McCullough ◽  
William K. Vencill
Keyword(s):  
Tall Fescue ◽  
Festuca Arundinacea ◽  
Creeping Bentgrass ◽  
Agrostis Stolonifera ◽  
Poa Annua ◽  
Physiological Effects ◽  
Cool Season ◽  
Foliar Absorption ◽  
Annual Bluegrass

Amicarbazone controls annual bluegrass in cool-season turfgrasses but physiological effects that influence selectivity have received limited investigation. The objective of this research was to evaluate uptake, translocation, and metabolism of amicarbazone in these species. Annual bluegrass, creeping bentgrass, and tall fescue required < 3, 56, and 35 h to reach 50% foliar absorption, respectively. At 72 h after treatment (HAT), annual bluegrass and creeping bentgrass translocated 73 and 70% of root-absorbed14C to shoots, respectively, while tall fescue only distributed 55%. Annual bluegrass recovered ≈ 50% more root-absorbed14C in shoots than creeping bentgrass and tall fescue. Creeping bentgrass and tall fescue metabolism of amicarbazone was ≈ 2-fold greater than annual bluegrass from 1 to 7 d after treatment (DAT). Results suggest greater absorption, more distribution, and less metabolism of amicarbazone in annual bluegrass, compared to creeping bentgrass and tall fescue, could be attributed to selectivity of POST applications.

Two new species of Clavaria (Agaricales, Basidiomycota) from Central China

Phytotaxa ◽  
2020 ◽  
Vol 477 (1) ◽  
pp. 71-80
Author(s):  
JUN YAN ◽  
XIAO-YAN WANG ◽  
XIAO-HUI WANG ◽  
ZUO-HONG CHEN ◽  
PING ZHANG
Keyword(s):  
Phylogenetic Analysis ◽  
New Species ◽  
Internal Transcribed Spacer ◽  
Close Relationships ◽  
Morphological Features ◽  
Its Sequences ◽  
Central China ◽  
Line Drawings ◽  
Two New Species ◽  
Normal University

Species of Clavaria (Clavariaceae, Agaricales) collected from China were studied using morphological and molecular methods. Two species, C. griseolilacina and C. sinensis were described as new species; both possess branched and pale purple-tinged fruit bodies. However, the former possess a few branched, while the latter is profusely branched. The phylogenetic analysis based on the internal transcribed spacer (ITS) sequences confirmed the distinctions between these two new species and their close relationships with C. rubicundula and C. fumosa in the genus. An evolutionary tendency in genus Clavaria was discussed. Additionally, morphological features of the two new species were described in detail and were illustrated with line drawings and photographs. Holotypes of the new species are deposited in the Mycological Herbarium of Hunan Normal University (MHHNU). The ITS sequences generated in this study were deposited in GenBank. A key is provided for the known Clavaria species in China.

scholarly journals Phylogenetic Analysis Reveals Multiple Lateral Transfers of Adenosine-5′-Phosphosulfate Reductase Genes among Sulfate-Reducing Microorganisms

2002 ◽  
Vol 184 (1) ◽  
pp. 278-289 ◽  
Author(s):  
Michael W. Friedrich
Keyword(s):  
Phylogenetic Analysis ◽  
Gene Transfer ◽  
16S Rrna ◽  
Lateral Gene Transfer ◽  
Phylogenetic Trees ◽  
Gram Positive ◽  
Sulfate Reducing ◽  
Wide Range ◽  
Aps Reductase ◽  
Key Enzyme

ABSTRACT Lateral gene transfer affects the evolutionary path of key genes involved in ancient metabolic traits, such as sulfate respiration, even more than previously expected. In this study, the phylogeny of the adenosine-5′-phosphosulfate (APS) reductase was analyzed. APS reductase is a key enzyme in sulfate respiration present in all sulfate-respiring prokaryotes. A newly developed PCR assay was used to amplify and sequence a fragment (∼900 bp) of the APS reductase gene, apsA, from a taxonomically wide range of sulfate-reducing prokaryotes (n = 60). Comparative phylogenetic analysis of all obtained and available ApsA sequences indicated a high degree of sequence conservation in the region analyzed. However, a comparison of ApsA- and 16S rRNA-based phylogenetic trees revealed topological incongruences affecting seven members of the Syntrophobacteraceae and three members of the Nitrospinaceae, which were clearly monophyletic with gram-positive sulfate-reducing bacteria (SRB). In addition, Thermodesulfovibrio islandicus and Thermodesulfobacterium thermophilum, Thermodesulfobacterium commune, and Thermodesulfobacterium hveragerdense clearly branched off between the radiation of the δ-proteobacterial gram-negative SRB and the gram-positive SRB and not close to the root of the tree as expected from 16S rRNA phylogeny. The most parsimonious explanation for these discrepancies in tree topologies is lateral transfer of apsA genes across bacterial divisions. Similar patterns of insertions and deletions in ApsA sequences of donor and recipient lineages provide additional evidence for lateral gene transfer. From a subset of reference strains (n = 25), a fragment of the dissimilatory sulfite reductase genes (dsrAB), which have recently been proposed to have undergone multiple lateral gene transfers (M. Klein et al., J. Bacteriol. 183:6028–6035, 2001), was also amplified and sequenced. Phylogenetic comparison of DsrAB- and ApsA-based trees suggests a frequent involvement of gram-positive and thermophilic SRB in lateral gene transfer events among SRB.

scholarly journals A suite of rare microbes interacts with a dominant, heritable, fungal endophyte to influence plant trait expression

2019 ◽  
Author(s):  
Joshua G. Harrison ◽  
Lyra P. Beltran ◽  
C. Alex Buerkle ◽  
Daniel Cook ◽  
Dale R. Gardner ◽  
...  
Keyword(s):  
Host Plants ◽  
Plant Size ◽  
Fungal Endophyte ◽  
Plant Tissues ◽  
Plant Leaves ◽  
Plant Trait ◽  
Astragalus Lentiginosus ◽  
Plant Phenotype ◽  
Foliar Pathogen ◽  
System Functioning

AbstractEndophytes are microbes that live, for at least a portion of their life history, within plant tissues. Endophyte assemblages are often composed of a few abundant taxa and many infrequently-observed, low-biomass taxa that are, in a word, rare. The ways in which most endophytes affect host phenotype are unknown; however, certain dominant endophytes can influence plants in ecologically meaningful ways–including by affecting growth and immune system functioning. In contrast, the effects of rare endophytes on their hosts have been unexplored, including how rare endophytes might interact with abundant endophytes to shape plant phenotype. Here, we manipulate both the suite of rare foliar endophytes (including both fungi and bacteria) and Alternaria fulva–a vertically-transmitted and usually abundant fungus–within the fabaceous forb Astragalus lentiginosus. We report that rare, low-biomass endophytes affected host size and foliar %N, but only when the heritable fungal endophyte (A. fulva) was not present. A. fulva also reduced plant size and %N, but these deleterious effects on the host could be offset by a negative association we observed between this heritable fungus and a foliar pathogen. These results demonstrate how interactions among endophytic taxa determine the net effects on host plants and suggest that the myriad rare endophytes within plant leaves may be more than a collection of uninfluential, commensal organisms, but instead have meaningful ecological roles.

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