scholarly journals phytanoyl-CoA dioxygenase domain-containing protein 1 plays an important role in egg shell formation of silkworm (Bombyx mori)

PLoS ONE ◽  
2021 ◽  
Vol 16 (12) ◽  
pp. e0261918
Author(s):  
Anli Chen ◽  
Pengfei Liao ◽  
Qiongyan Li ◽  
Qiaoling Zhao ◽  
Mengjie Gao ◽  
...  
Keyword(s):  
Sequence Analysis ◽  
Genetic Analysis ◽  
Target Gene ◽  
Z Chromosome ◽  
Hatching Rate ◽  
Chromosome 2 ◽  
Shell Formation ◽  
Chorion Genes ◽  
Egg Shell ◽  
Silkworm Bombyx Mori

Yun7Ge is a giant egg mutant found in the silkworm variety Yun7. In comparison with the giant mutant Ge, the eggs of Yun7Ge are larger. The number of laid eggs and hatching rate of Yun7Ge are reduced, which is not conducive to reproduction. In this work, the target gene controlling giant egg trait is located on the Z chromosome and was determined through genetic analysis. Transcriptome results showed that phytanoyl-CoA dioxygenase domain-containing protein 1 (PHYHD1) on the Z chromosome was silenced, and the 25 chorion genes on chromosome 2 were remarkably downregulated. Sequence analysis showed that the 73.5 kb sequence including the PHYHD1 was replaced by a ~3.0 kb sequence. After knocking out the PHYHD1 by using CRISPR/Cas9, the chorion genes were significantly downregulated. Hence, the silencing of PHYHD1 leads to the downregulation of many chorion protein genes, thus directly causing giant eggs.

scholarly journals The Effect of Lithium Carbonate on Egg Shell Formation and Serum Calcium Level of the Hen

1971 ◽  
Vol 50 (2) ◽  
pp. 577-580 ◽  
Author(s):  
R.D. Creek ◽  
Pauline Lund ◽  
O.P. Thomas ◽  
W.O. Pollard
Keyword(s):  
Serum Calcium ◽  
Calcium Level ◽  
Serum Calcium Level ◽  
Lithium Carbonate ◽  
Shell Formation ◽  
Egg Shell

Role of magnesium in egg shell formation in the domestic hen

1991 ◽  
Vol 32 (4) ◽  
pp. 853-864 ◽  
Author(s):  
A. L. Waddell ◽  
R. G. Board ◽  
V. D. Scott ◽  
S. G. Tullett
Keyword(s):  
Shell Formation ◽  
Egg Shell ◽  
Domestic Hen

SULFONAMIDES AND EGG-SHELL FORMATION IN THE DOMESTIC FOWL

Science ◽  
1945 ◽  
Vol 101 (2633) ◽  
pp. 617-618 ◽  
Author(s):  
R. BERNARD ◽  
P. GENEST
Keyword(s):  
Domestic Fowl ◽  
Shell Formation ◽  
Egg Shell

scholarly journals Molecular and genetic analysis of the yeast early meiotic recombination genes REC102 and REC107/MER2.

1992 ◽  
Vol 12 (3) ◽  
pp. 1248-1256 ◽  
Author(s):  
M Cool ◽  
R E Malone
Keyword(s):  
Sequence Analysis ◽  
Genetic Analysis ◽  
Meiotic Recombination ◽  
Genetic Recombination ◽  
Recombination Process ◽  
Genetic Complementation ◽  
Rec Genes ◽  
Reductional Division

By selecting for mutations which could rescue the meiotic lethality of a rad52 spo13 strain, we isolated several new Rec genes required relatively early in the meiotic recombination process. This paper presents data to confirm that two of them, REC102 and REC107, are general, meiosis-specific recombination genes that have no detectable role during mitosis. Sequence analysis and genetic complementation indicate that REC107 is identical to the MER2 gene. No sequences related to REC102 have been found in the GenBank or EMBL collections. REC102 is expressed only in meiosis, prior to the reductional division, at about the time that genetic recombination occurs. Examination of the REC102 sequence indicates the presence of several sequences which may play a role in the regulation of its expression; however, the URS1 sequence commonly found in genes expressed early in meiosis is not present.

scholarly journals DNA SEQUENCES OF FRAMESHIFT AND OTHER MUTATIONS INDUCED BY ICR-170 IN YEAST

Genetics ◽  
1985 ◽  
Vol 111 (2) ◽  
pp. 233-241
Author(s):  
Joachim F Ernst ◽  
D Michael Hampsey ◽  
Fred Sherman
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Amino Acid ◽  
Sequence Analysis ◽  
Genetic Analysis ◽  
Dna Sequences ◽  
Induced Mutations ◽  
Sequence Analyses ◽  
Base Pairs ◽  
Yeast Saccharomyces Cerevisiae ◽  
Frameshift Mutations

ABSTRACT ICR-170-induced mutations in the CYC1 gene of the yeast Saccharomyces cerevisiae were investigated by genetic and DNA sequence analyses. Genetic analysis of 33 cyc1 mutations induced by ICR-170 and sequence analysis of eight representatives demonstrated that over one-third were frameshift mutations that occurred at one site corresponding to amino acid positions 29-30, whereas the remaining mutations were distributed more-or-less randomly, and a few of these were not frameshift mutations. The sequence results indicate that ICR-170 primarily induces G·C additions at sites containing monotonous runs of three G·C base pairs. However, some (see PDF) sites within the CYC1 gene were not mutated by ICR-170. Thus, ICR-170 is a relatively specific mutagen that preferentially acts on certain sites with monotonous runs of G·C base pairs.

scholarly journals Effects of Pyriproxyfen Exposure on Reproduction and Gene Expressions in Silkworm, Bombyx mori

Insects ◽  
2020 ◽  
Vol 11 (8) ◽  
pp. 467
Author(s):  
He-Ying Qian ◽  
Xiao Zhang ◽  
Guo-Dong Zhao ◽  
Hui-Min Guo ◽  
Gang Li ◽  
...  
Keyword(s):  
Bombyx Mori ◽  
Model Organism ◽  
Ovary Development ◽  
Control Group ◽  
Hatching Rate ◽  
Hormone Regulation ◽  
Concentration Effects ◽  
Gene Expressions ◽  
Silkworm Larvae ◽  
Silkworm Bombyx Mori

The silkworm, Bombyx mori Linnaeus, is an important economic insect and a representative model organism of Lepidoptera, which has been widely used in the study of reproduction and development. The development of the silkworm’s reproductive gland is easily affected by many external factors, such as chemical insecticides. After the silkworm larvae were treated with different concentrations of pyriproxyfen, the results showed that the number of eggs and hatching rate of eggs in the silkworm can be reduced by pyriproxyfen, and the concentration effects were displayed. Pyriproxyfen exposure could affect the normal development of the ovary tissue by reducing the number of oocytes and oogonia in the ovaries of silkworm fed with pyriproxyfen. We employed qRT-PCR, to detect the expressions of genes related to ovary development (Vg, Ovo, Otu, Sxl-S and Sxl-L) and hormone regulation (EcR and JHBP2) in silkworm. Our study showed that the transcription levels of Vg, Ovo, Otu, Sxl-S and Sxl-L in the treatment group were lower than those in the control group (6.08%, 61.99%, 83.51%, 99.31% and 71.95%, respectively). The transcription level of ECR was 70.22% for the control group, while that of JHBP2 was upregulated by 3.92-fold. Changes of transcription levels of these genes caused by pyriproxyfen exposure ultimately affect the absorption of nutrients, energy metabolism, ovary development and egg formation of the silkworm, thus leading to reproductive disorders of the silkworm. In general, our study revealed the response of silkworm reproduction to pyriproxyfen exposure and provided a certain reference value for the metabolism of the silkworm to pyriproxyfen.

Studies on the structure of the female reproductive system and egg-shell formation inAspiculuris tetrapteraSchulz, (Nematoda: Oxyuroidea)

Parasitology ◽  
1964 ◽  
Vol 54 (4) ◽  
pp. 699-719 ◽  
Author(s):  
A. O. Anya
Keyword(s):  
Reproductive System ◽  
Shell Formation ◽  
Female Reproductive System ◽  
High Concentration ◽  
Classical Study ◽  
Quinone Tanning ◽  
Phenolic Substances ◽  
Egg Shell ◽  
Exogenous Origin ◽  
Aspiculuris Tetraptera

The histological anatomy of the female reproductive system of an oxyuroid nematode,Aspiculuris tetraptera, Schulz, has been described.The process of egg-shell formation in this animal has been followed in detail while the structural and chemical characteristics of the egg-shell have been studied by histochemical and other methods. It is shown that there are three layers: a lipoprotein layer, a ‘chitinous’ layer and the so-called vitelline (glycosidal) membrane. Evidence is presented for the exogenous origin of the lipoprotein layer: this being formed by the cells of the upper uterus which are shown to be secretory.The question of quinone-tanning in the egg-shell ofA. tetrapteraand in other oxyuroids and ascarids is considered. It is shown that neither a polyphenol oxidase nor a high concentration of phenolic substances (apart from protein tyrosine) exists in this system. The significance of these and other observations is discussed in relation to the mechanism of tanning as elucidated in insects and trematodes.I have to acknowledge with gratitude the support of many during these investigations. To Dr P. Tate for his encouragement and provision of facilities at the Molteno Institute; to Dr D. L. Lee, for much useful discussion and permission to refer to some of his unpublished electron micrographs of nematodes; to Professor J. D. Smyth, who kindly read through the manuscript; to the Cambridge Philosophical Society for a grant that made possible the translation of Fauré-Frémiet's classical study onAscaris;and to the Department of Technical Cooperation for financial assistance.

scholarly journals Intracellular pH during calcification. A study of the avian shell gland

1969 ◽  
Vol 111 (5) ◽  
pp. 647-652 ◽  
Author(s):  
K. Simkiss
Keyword(s):  
Intracellular Ph ◽  
Domestic Fowl ◽  
Shell Gland ◽  
Shell Formation ◽  
Extracellular Water ◽  
Rapid Fall ◽  
Egg Shell

The intracellular pH of the shell gland of the domestic fowl was calculated at various stages in egg-shell formation. The calculation is based on the distribution of 5,5-dimethyloxazolidine-2,4-dione between intracellular and extracellular water. The results show a rapid fall in intracellular pH at the time of mineralization and this is interpreted as indicating a removal of protons from the site of calcification.

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