scholarly journals Trolox-Equivalent Antioxidant Capacity Assay Versus Oxygen Radical Absorbance Capacity Assay in Plasma

2004 ◽  
Vol 50 (5) ◽  
pp. 952-954 ◽  
Author(s):  
Chi Chiu Wang ◽  
Ching Yan Chu ◽  
Kai On Chu ◽  
Kwong Wai Choy ◽  
Kim Sun Khaw ◽  
...  
Keyword(s):  
Antioxidant Capacity ◽  
Oxygen Radical ◽  
Oxygen Radical Absorbance Capacity ◽  
Trolox Equivalent Antioxidant Capacity ◽  
Trolox Equivalent

scholarly journals Determination of antioxidant capacity in some foods by oxygen radical absorbance capacity (ORAC)

2021 ◽  
Vol 4 (3) ◽  
pp. 36-46
Author(s):  
Thuy Le Thi ◽  
Thuong Nguyen Nhu ◽  
Bang Nguyen Thi ◽  
Truc Quynh Do ◽  
Trang Vu Thi ◽  
...  
Keyword(s):  
Antioxidant Capacity ◽  
High Selectivity ◽  
Peroxyl Radical ◽  
Fluorescence Decay ◽  
Oxygen Radical ◽  
Oxygen Radical Absorbance Capacity ◽  
Linear Coefficient ◽  
Vegetable Products ◽  
Trolox Equivalent

Oxygen radical absorbance capacity (ORAC) assay has been applied to determine the Hydrophilic – ORAC index in food. The method measures antioxidant scavenging activity against peroxyl radical induced by 2,2′-azobis(2-amidino-propane) dihydrochloride (AAPH) at 37ºC, used fluororescein as the fluorescent probe. The antioxidant capacity is measured by assemssing the fluorescence decay curve (AUC) of the sample as compared to that of the blank in which no antioxidant is present. Results expressed in ORAC units, equivalent to micromole Trolox per 100 grams sample (Trolox equivalent). The method was shown with high selectivity, a wide linear range, from 5 to 50µM Trolox with linear coefficient R2 = 0.9987. The recovery from 93.2% to 104.4% and repeatability (RSD) was less than 6.60%. The limits of detection and quantitation were 5 and 10µM Trolox, respectively. The method has been applied to determine of H-ORAC index in 65 samples including vegetables, fruits, vegetable products and health supplements with content ranging from 720 µM TE/100g to 310878 µM TE/100g.

The effect of the trolox equivalent antioxidant capacity (TEAC) in plasma on the formation of 4-aminobiphenylhaemoglobin adducts in smokers

Biomarkers ◽  
2002 ◽  
Vol 7 (4) ◽  
pp. 291-298 ◽  
Author(s):  
Jan W. Dallinga ◽  
Guido R. M. M. Haenen ◽  
Aalt Bast ◽  
Frederik-Jan Van Schooten
Keyword(s):  
Antioxidant Capacity ◽  
Trolox Equivalent Antioxidant Capacity ◽  
Trolox Equivalent

scholarly journals Effect of the French Oak Wood Extract Robuvit on Markers of Oxidative Stress and Activity of Antioxidant Enzymes in Healthy Volunteers: A Pilot Study

2014 ◽  
Vol 2014 ◽  
pp. 1-6 ◽  
Author(s):  
Martina Horvathova ◽  
Zuzana Orszaghova ◽  
Lucia Laubertova ◽  
Magdalena Vavakova ◽  
Peter Sabaka ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Enzymes ◽  
Antioxidant Capacity ◽  
Healthy Volunteers ◽  
Total Antioxidant Capacity ◽  
Trolox Equivalent Antioxidant Capacity ◽  
Total Antioxidant ◽  
Trolox Equivalent ◽  
Markers Of Oxidative Stress

We examinedin vitroantioxidant capacity of polyphenolic extract obtained from the wood of oakQuercus robur(QR), Robuvit, using TEAC (Trolox equivalent antioxidant capacity) method and the effect of its intake on markers of oxidative stress, activity of antioxidant enzymes, and total antioxidant capacity in plasma of 20 healthy volunteers. Markers of oxidative damage to proteins, DNA, and lipids and activities of Cu/Zn-superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx) were determined in the erythrocytes. We have found anin vitroantioxidant capacity of Robuvit of 6.37 micromole Trolox equivalent/mg of Robuvit. One month intake of Robuvit in daily dose of 300 mg has significantly decreased the serum level of advanced oxidation protein products (AOPP) and lipid peroxides (LP). Significantly increased activities of SOD and CAT as well as total antioxidant capacity of plasma after one month intake of Robuvit have been shown. In conclusion, we have demonstrated for the first time that the intake of Robuvit is associated with decrease of markers of oxidative stress and increase of activity of antioxidant enzymes and total antioxidant capacity of plasmain vivo.

Effect of Propofol, Sevoflurane and Desflurane on Systemic Redox Balance

2007 ◽  
Vol 20 (3) ◽  
pp. 585-593 ◽  
Author(s):  
G. Cinnella ◽  
G. Vendemiale ◽  
M. Dambrosio ◽  
G. Serviddio ◽  
P.L. Pugliese ◽  
...  
Keyword(s):  
Antioxidant Capacity ◽  
Antioxidant Properties ◽  
Redox Balance ◽  
Trolox Equivalent Antioxidant Capacity ◽  
Sodium Thiopental ◽  
Blood Samples ◽  
Plasma Antioxidant Capacity ◽  
Trolox Equivalent ◽  
Plasma Antioxidant ◽  
Group D

We studied the effects of Propofol, Desflurane, and Sevoflurane on the systemic redox balance in patients undergoing laparohysterectomy. We measured blood concentration of glutathione (GSH), plasma antioxidant capacity (Trolox Equivalent Antioxidant Capacity-TEAC), and lipid peroxidation products (malondialdehyde (aMDA) and 4-hydroxynonenal (aHNE) protein adducts). Sixty patients were randomly placed into three groups of twenty people each. In Group P anesthesia was induced with Propofol 2 mg/kg and maintained with 12–10–8 mg/kg/min; in Groups S and D anesthesia was induced with 3 mg/kg Sodium Thiopental and maintained with 2% Sevoflurane and 6% Desflurane, respectively. Blood samples were collected prior to induction (T0bas), 60min and 24h postoperatively (T160' and T224 h). In Group P, GSH increased on T160' (p<0.02) and returned to baseline on T24h, while TEAC remained unmodified; in Groups S, GSH and TEAC decreased on T160' (p<0.01 vs. T0bas, p<0.03 vs. T0bas, respectively); in Group D, on T160' there was a slight decrease of GSH and TEAC. The levels of aMDA slightly decreased throughout the study periods in Group P, increased in Group D, and remained stable in Group S. Propofol showed antioxidant properties, while Sevoflurane and Desflurane seemed to shift the redox balance towards oxidation, yet without inducing overt oxidative damage.

scholarly journals Changes in Markers of Oxidative Stress and α-Amylase in Saliva of Children Associated with a Tennis Competition

2020 ◽  
Vol 17 (17) ◽  
pp. 6269 ◽  
Author(s):  
José María Giménez-Egido ◽  
Raquel Hernández-García ◽  
Damián Escribano ◽  
Silvia Martínez-Subiela ◽  
Gema Torres-Luque ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Capacity ◽  
Psychological Stress ◽  
Antioxidant Status ◽  
Perceived Exertion ◽  
Progressive Increase ◽  
Rating Of Perceived Exertion ◽  
Trolox Equivalent Antioxidant Capacity ◽  
Reducing Ability ◽  
Trolox Equivalent

The purpose of this paper was to analyze the changes caused by a one-day tennis tournament in biomarkers of oxidative stress and α-amylase in saliva in children. The sample was 20 male active children with the following characteristics: (a) age of players = 9.46 ± 0.66 years; (b) weight = 34.8 ± 6.5 kg; (c) height = 136.0 ± 7.9 cm; (d) mean weekly training tennis = 2.9 ± 1.0 h. The tennis competition ran for one day, with four matches for each player. Data were taken from the average duration per match and the rating of perceived exertion (RPE). Four biomarkers of antioxidant status: uric acid (AU), Trolox equivalent antioxidant capacity (TEAC), ferric reducing ability of saliva (FRAS, cupric reducing antioxidant capacity (CUPRAC) and salivary alpha-amylase (sAA) as a biomarker of psychological stress were measured in saliva. The time points were baseline (at home before the tournament), pre-competition (immediately before the first match) and post-match (after each match) measurements. The four biomarkers of antioxidant status showed a similar dynamic with lower values at baseline and a progressive increase during the four matches. Overall one-day tennis competition in children showed a tendency to increase antioxidant biomarkers in saliva. In addition, there was an increase in pre-competition sAA possibly associated with psychological stress. Further studies about the possible physiological implications of these findings should be performed in the future.

scholarly journals Evaluation of theIn VitroandIn VivoAntioxidant Potentials ofSudarshanaPowder

2018 ◽  
Vol 2018 ◽  
pp. 1-5 ◽  
Author(s):  
Weerakoon Achchige Selvi Saroja Weerakoon ◽  
Pathirage Kamal Perera ◽  
Dulani Gunasekera ◽  
Thusharie Sugandhika Suresh
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Capacity ◽  
Thiobarbituric Acid ◽  
Trolox Equivalent Antioxidant Capacity ◽  
Control Group ◽  
Thiobarbituric Acid Reactive Substance ◽  
Trolox Equivalent ◽  
Antioxidant Effects

Sudarshanapowder (SP) is one of the most effective Ayurveda powder preparations for paediatric febrile conditions. The objective of the present study was to evaluate thein vitroandin vivoantioxidant potentials of SP. Thein vitroantioxidant effects were evaluated using ABTS radical cation decolourization assay where the TROLOX equivalent antioxidant capacity (TEAC) was determined. Thein vivoantioxidant activity of SP was determined in Wistar rats using the Lipid Peroxidation (LPO) assay in serum. Thein vitroassay was referred to as the TROLOX equivalent antioxidant capacity (TEAC) assay. For thein vivoassay, animals were dosed for 21 consecutive days and blood was drawn to evaluate the MDA level. Thein vitroantioxidant activity of 0.5 μg of SP was equivalent to 14.45 μg of standard TROLOX. The percentage inhibition against the radical formation was50.93±0.53%. The SP showed a statistically significant (p<0.01) decrease in the serum level of thiobarbituric acid-reactive substance in the test rats when compared with the control group. These findings suggest that the SP possesses potent antioxidant activity which may be responsible for some of its reported bioactivities.

scholarly journals PSI-36 Effect of short-chain fructooligosaccharides on Trolox equivalent antioxidant capacity and thiobarbituric acid reactive substances in mature and senior stock-type horses

2019 ◽  
Vol 97 (Supplement_3) ◽  
pp. 254-255
Author(s):  
Emili McClure ◽  
Courtney P Heaton ◽  
Dishnu Sajeev ◽  
Thu Dinh
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Capacity ◽  
Random Effect ◽  
Thiobarbituric Acid ◽  
Short Chain ◽  
Trolox Equivalent Antioxidant Capacity ◽  
Thiobarbituric Acid Reactive Substances ◽  
Stress Markers ◽  
Trolox Equivalent ◽  
Stock Type

Abstract Oxidative stress (OS) causes health complications through the destruction of cellular components as individuals age. Reactive oxygen species are used to measure OS through Trolox equivalent antioxidant capacity (TEAC) and thiobarbituric acid reactive substances (TBARS). Other prebiotics have been used to reduce OS markers in numerous species; however, the effect of short-chain fructooligosaccharides (scFOS) on OS has not been studied in the horse. Ten healthy stock-type horses were blocked by age into 2 groups: mature (MA; n = 5; 7.0 ± 0.87 yr) and senior (SR; n = 5; 22.6 ± 1.1 yr) to analyze effects of scFOS on TEAC and TBARS. Horses were randomly assigned to 1 of 3 diets for 25 d before transition to another diet. Diets were bermudagrass hay offered at 1.5% BW/d hay as-fed, hay with a ration balancer (CON), or hay with a ration balancer and scFOS added at a rate of 2.5 g/kg (PRE). Prior to a total fecal collection for an alternate study, horses were fasted overnight for 8 h with blood samples taken immediately prior to feeding (0), 30, and 60 min postprandial. Oxidative stress markers were analyzed for the 2 ration balancer diets. Statistical analysis was performed with SAS using the MIXED procedure with horse within diet as a random effect with significance of P ≤ 0.05. Trolox equivalent antioxidant capacity was unaffected by diet (P = 0.827) or age (P = 0.347). Time (P = 0.006) was significant for TBARS which increased postprandial regardless of treatment or age. Consistent with other species, higher levels of OS was found in SR compared to MA regardless of time or diet (P = 0.037; 4.491 µM vs. 3.412 µM TBARS, respectively). These results indicate that scFOS do not seem to be effective in reducing OS in SR and MA horses.

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