scholarly journals Effect of Blood Collection Tubes on Total Triiodothyronine and Other Laboratory Assays

2005 ◽  
Vol 51 (2) ◽  
pp. 424-433 ◽  
Author(s):  
Raffick AR Bowen ◽  
Yung Chan ◽  
Joshua Cohen ◽  
Nadja N Rehak ◽  
Glen L Hortin ◽  
...  
Keyword(s):  
Clinical Chemistry ◽  
Reference Interval ◽  
Blood Collection ◽  
Serum Samples ◽  
Becton Dickinson ◽  
Total Triiodothyronine ◽  
Patient Values ◽  
Clinically Significant ◽  
Age Range ◽  
Blood Collection Tubes

Abstract Background: Increased total triiodothyronine (TT3) assay results in apparently euthyroid patients triggered an investigation of the effect of blood collection tubes on serum TT3 and other laboratory assays. Methods: We examined potential assay interference for three types of tubes: plastic Greiner Bio-One™ Vacuette™; glass Becton Dickinson (BD) Vacutainer™; and plastic BD Vacutainer SST™ tubes. Serum samples from apparently healthy volunteers (age range, 30–60 years; 15 males and 34 females) were collected in different tube types and analyzed in 17 immunoassays (n = 49), 30 clinical chemistry tests (n = 20), and 33 immunology assays (n = 15). Tube effects were also examined by adding pooled serum to different tube types. Results: TT3 values, when measured by the IMMULITE™ 2000 but not the AxSYM™ analyzer, were significantly higher (P <0.0001) for SST (2.81 nmol/L) than either glass (2.15 nmol/L) or Vacuette (2.24 nmol/L) tubes. The effect was large enough to substantially shift the distribution of patient values, increasing the percentage of values above the reference interval from 11.3% to 35.8%. The degree of interference from SST tubes on TT3 differed among various tube lots and could be attributed to a tube additive shared by other plastic tubes. Results from several other tests statistically differed among tube types, but differences were not considered to be clinically significant. Conclusions: Assay interferences from blood collection tubes represent challenges to clinical laboratories because they are not detected by the usual quality-control or proficiency testing programs. Laboratories can, however, address this problem by monitoring distribution of patients’ results.

Comparison of some biochemical tests in different blood collection tubes in hemodialysis patients

2019 ◽  
Vol 45 (1) ◽  
pp. 26-36 ◽  
Author(s):  
Arzu Kösem ◽  
Canan Topçuoğlu ◽  
Sevilay Sezer ◽  
Şimal Köksal Cevher ◽  
Ezgi Coşkun Yenigün ◽  
...  
Keyword(s):  
Clinical Chemistry ◽  
Statistical Significance ◽  
Blood Collection ◽  
Test Results ◽  
Biochemical Tests ◽  
Becton Dickinson ◽  
Two Samples ◽  
Clinically Significant ◽  
Roche Diagnostics ◽  
Blood Collection Tubes

Abstract Objective Blood collection tubes (BCTs) related interferences in test results can adversely influence on patient outcomes. We compared test results of samples in BD (Becton-Dickinson, Franklin Lakes, NJ, USA) Vacutainer Serum Separator Tubes (SST), BD Vacutainer® Barricor™ Plasma BCTs (Barricor™) and BD Vacutainer® Rapid Serum Tube (RST). Materials and methods Thirty-two samples were obtained from patients after the hemodialysis were included in this study. Eight routine clinical chemistry parameters (AST, creatinin, urea, PTH, glucose, LDH, K, calcium) were measured on Roche Cobas Analyzer (Roche Diagnostics, North America). The results of samples obtained from RST and Barricor™ were compared with SST as reference tubes. Results Results of Glucose, K, Urea, PTH from the SST and Barricor™ were statistically significantly different (p = 0.017, p < 0.001, p = 0.011, p < 0.001, respectively). In addition, results of PTH, LDH from SST and RST were significantly different (p < 0.001, p = 0.019). However, statistical significance of test results was not clinically significant for the biochemical parameters. Conclusion Working with Barricor™ may provide not just a fast, clean, high-quality plasma samples, safety results, but also time and cost-effectivity. Therefore, these types of tubes, which are less costly than other BCTs, may be preferred to obtain plasma.

Estimation, prevention, and quality control of carbon dioxide loss during aerobic sample processing.

1981 ◽  
Vol 27 (10) ◽  
pp. 1676-1681 ◽  
Author(s):  
Z L Bandi
Keyword(s):  
Carbon Dioxide ◽  
Quality Control ◽  
Control Program ◽  
Blood Collection ◽  
Serum Samples ◽  
Quality Control Program ◽  
Control Programs ◽  
Tert Butylamine ◽  
Clinically Significant ◽  
Blood Collection Tubes

Abstract We find that 2 to 6 mmol of carbon dioxide per liter (mean: 4.1 mmol/L) is lost during routine laboratory processing of patients' serum samples after centrifugation. Additional CO2 may be lost if evacuated blood-collection tubes are not filled completely during phlebotomy. More than 2 mmol of CO2 per liter is lost from samples stored in tightly stoppered tubes for 120 min if the tubes are less than half full. In extreme cases, 8 mmol of CO2 per liter may be lost from samples exposed to room air in open cups of automated micro-sample instruments. Clinically significant CO2 loss (greater than 2 mmol/L) before analysis is not detected by many laboratories because the generally accepted quality-control programs monitor only the very last step of the analytical process. A valid CO2 quality-control program should include samples with high as well as the generally used low pCO2 values. Alkalinization of serum and plasma samples with tert-butylamine prevents CO2 loss. Optimum tert-butylamine concentration, pH, and pCO2 were about 14 to 16 mmol/L, 9.0 to 9.3, and 0.4 to 1.5 mmHg (about 50 to 200 Pa).

scholarly journals Immunoassay Interference by a Commonly Used Blood Collection Tube Additive, the Organosilicone Surfactant Silwet L-720

2005 ◽  
Vol 51 (10) ◽  
pp. 1874-1882 ◽  
Author(s):  
Raffick AR Bowen ◽  
Yung Chan ◽  
Mark E Ruddel ◽  
Glen L Hortin ◽  
Gyorgy Csako ◽  
...  
Keyword(s):  
Solid Phase ◽  
Blood Collection ◽  
Becton Dickinson ◽  
Total Triiodothyronine ◽  
Collection Tube ◽  
Capture Antibody ◽  
Blood Collection Tubes ◽  
Blood Collection Tube ◽  
Selection Of ◽  
Serum Components

Abstract Background: A small number of immunoassays on several different types of analyzers were recently adversely affected by tube additives in Becton Dickinson (BD) Vacutainer® SST™, SST II, and Microtainer™ blood collection tubes. We examined the effect of a commonly used tube surfactant, Silwet™ L-720, on immunoassays and the mechanism for the interference. Methods: Immunoassays were performed on serum supplemented with Silwet L-720 on the IMMULITE™ 2500 and AxSYM™ analyzers. Direct effects of the surfactant on the chemiluminescent detection step of immunoassays and on antibody immobilization on the solid phase were examined. Results: Increasing the final surfactant concentration from 0 to 400 mg/L in serum significantly increased (∼51%) the apparent total triiodothyronine (TT3) concentrations measured on the IMMULITE 2500 but not the AxSYM analyzer. Several other competitive, but not noncompetitive, assays were also significantly affected by the surfactant on the IMMULITE 2500 analyzer. The effect was independent of serum components, and the surfactant had no direct effect on chemiluminescence reactions. The capture antibody, however, was displaced from the solid phase by incubation with solutions containing surfactant under conditions similar to the IMMULITE TT3 assay. Conclusions: The Silwet L-720 surfactant, which is used to coat the inner surfaces of tubes, appears to account for previously reported immunoassay interference by BD Vacutainer SST blood collection tubes. One of the mechanisms for the interference is the desorption of antibodies from the solid phase by the surfactant. The results identify an important factor in the selection of suitable blood collection tube surfactants and provide an approach for solving similar tube-assay interference problems in the future.

scholarly journals Clinical Evaluation of the Torq Zero Delay Centrifuge System for Decentralized Blood Collection and Stabilization

Diagnostics ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 1019
Author(s):  
Kyungjin Hong ◽  
Gabriella Iacovetti ◽  
Ali Rahimian ◽  
Sean Hong ◽  
Jon Epperson ◽  
...  
Keyword(s):  
Clinical Chemistry ◽  
Blood Collection ◽  
Test Results ◽  
Sample Collection ◽  
Rapid Separation ◽  
Cell Counts ◽  
Collection Device ◽  
Precision Study ◽  
Clinically Significant ◽  
Blood Specimens

Blood sample collection and rapid separation—critical preanalytical steps in clinical chemistry—can be challenging in decentralized collection settings. To address this gap, the Torq™ zero delay centrifuge system includes a lightweight, hand-portable centrifuge (ZDrive™) and a disc-shaped blood collection device (ZDisc™) enabling immediate sample centrifugation at the point of collection. Here, we report results from clinical validation studies comparing performance of the Torq System with a conventional plasma separation tube (PST). Blood specimens from 134 subjects were collected and processed across three independent sites to compare ZDisc and PST performance in the assessment of 14 analytes (K, Na, Cl, Ca, BUN, creatinine, AST, ALT, ALP, total bilirubin, albumin, total protein, cholesterol, and triglycerides). A 31-subject precision study was performed to evaluate reproducibility of plasma test results from ZDiscs, and plasma quality was assessed by measuring hemolysis and blood cells from 10 subject specimens. The ZDisc successfully collected and processed samples from 134 subjects. ZDisc results agreed with reference PSTs for all 14 analytes with mean % biases well below clinically significant levels. Results were reproducible across different operators and ZDisc production lots, and plasma blood cell counts and hemolysis levels fell well below clinical acceptance thresholds. ZDiscs produce plasma samples equivalent to reference PSTs. Results support the suitability of the Torq System for remotely collecting and processing blood samples in decentralized settings.

Preanalytical robustness of blood collection tubes with RNA stabilizers

2019 ◽  
Vol 57 (10) ◽  
pp. 1522-1529 ◽  
Author(s):  
Chiara Stellino ◽  
Gaël Hamot ◽  
Camille Bellora ◽  
Johanna Trouet ◽  
Fay Betsou
Keyword(s):  
Storage Temperature ◽  
Regulation Of Gene Expression ◽  
Analysis Data ◽  
Blood Collection ◽  
Becton Dickinson ◽  
Rna Integrity ◽  
Qrt Pcr ◽  
Rna Integrity Number ◽  
Significant Difference ◽  
Blood Collection Tubes

Abstract Background Efficient blood stabilization is essential to obtaining reliable and comparable RNA analysis data in preclinical operations. PAXgene (Qiagen, Becton Dickinson) and Tempus (Applied Biosystems, Life Technologies) blood collection tubes with RNA stabilizers both avoid preanalytical degradation of mRNA by endogenous nucleases and modifications in specific mRNA concentrations by unintentional up- or down-regulation of gene expression. Methods Sixteen different preanalytical conditions were tested in PAXgene and Tempus blood samples from seven donors: different mixing after collection, different fill volumes and different 24-h transport temperature conditions after collection. RNA was extracted by column-based methods. The quality of the extracted RNA was assessed by spectrophotometric quantification, A260/A280 purity ratio, RNA Integrity Number (Agilent Bioanalyzer), miRNA quantative real time polymerase chain reaction (qRT-PCR) on two target miRNAs (RNU-24 and miR-16), mRNA quality index by qRT-PCR on the 3′ and 5′ region of the GAPDH gene, and the PBMC preanalytical score, based on the relative expression levels of the IL8 and EDEM3 coding genes. Results When PAXgene RNA and Tempus blood collection tubes were used following the manufacturers’ instructions, there was no statistically or technically significant difference in the output RNA quality attributes. However, the integrity of the RNA extracted from Tempus collection tubes was more sensitive to fill volumes and effective inversion, than to storage temperature, while the integrity of RNA extracted from PAXgene collection tubes was more sensitive to effective inversion and storage temperature than to fill volumes. Conclusions Blood collection tubes with different RNA stabilizers present different robustness to common preanalytical variations.

Clinical Evaluation of Kinetic Enzymatic Fixed-Time and Integral Analysis of Serum Triglycerides

1974 ◽  
Vol 20 (4) ◽  
pp. 476-481 ◽  
Author(s):  
T O Tiffany ◽  
J M Morton ◽  
E M Hall ◽  
A S Garrett
Keyword(s):  
Serum Alkaline Phosphatase ◽  
Fixed Time ◽  
Normal Serum ◽  
Blood Collection ◽  
Fasting Serum ◽  
Serum Samples ◽  
Time Rate ◽  
Serum Triglycerides ◽  
Hydrolysis Of ◽  
Blood Collection Tubes

Abstract Serum triglycerides can be enzymatically determined by hydrolysis of triglycerides to glycerol and free fatty acids [Clin. Chem. 19, 476 (1973)]. Enzyme integral (end-point) and fixed-time rate analyses of serum triglycerides by this procedure have been investigated with a centrifugal analyzer. We determined the precision of blank determinations, the contribution of normal serum alkaline phosphatase to total values, and the amount of endogenous glycerol in fasting serum samples and blood collection tubes. We also made a statistical analysis of enzymatic triglyceride values obtained for a healthy, fasting (12-16 h) population of men and women. The relative merits of both procedures are discussed.

Comparison of Improvacuter™ tubes with BD Vacutainer™ tubes for various hormones in the aspects of stability and influence of gel separators

2015 ◽  
Vol 53 (2) ◽  
Author(s):  
Cevdet Zungun ◽  
Fatma MeriÇ Yılmaz ◽  
Elif Guney Boru ◽  
Canan Topcuoglu
Keyword(s):  
Statistical Significance ◽  
Blood Collection ◽  
Laboratory Results ◽  
Analytical Range ◽  
Clinically Significant ◽  
Lower Limits ◽  
Blood Collection Tubes ◽  
Clot Activator

AbstractValidation of blood collection tubes are important to determine the role of different collection tubes which influence the assurance of laboratory results. We compared two different tubes (ImprovacuterWe compared the results of nine immunoassays performed on UniCelEstradiol and testosterone concentrations obtained from Improvacuter Gel and Clot Activator tubes and BD Vacutainer SST II Advance tubes remained below the lower limits of analytical range for the same analytes while they were within the limits in BD Vacutainer Clot Activator tubes and Improvacuter tubes. Statistical significance of stability was not clinically significant for the hormone parameters we tested in all four tubes.Gel containing tubes (both BD and Improve) gave comparable results with the tubes which do not contain gel except for estradiol and testosterone. The use of gel containing tubes for estradiol and testosterone are not recommended on UniCel

scholarly journals Simple thrombin-based method for eliminating fibrinogen interference in serum protein electrophoresis of haemodialysed patients

2020 ◽  
Vol 30 (2) ◽  
pp. 265-271
Author(s):  
Anamarija Rade ◽  
Anamarija Đuras ◽  
Irena Kocijan ◽  
Patricija Banković Radovanović ◽  
Ana Turčić
Keyword(s):  
Serum Protein ◽  
Venous Catheter ◽  
Protein Electrophoresis ◽  
Serum Protein Electrophoresis ◽  
Blood Collection ◽  
Serum Samples ◽  
Becton Dickinson ◽  
Vascular Access Devices ◽  
Hospital Protocols ◽  
Clot Activator

Introduction: Serum samples of haemodialysed patients collected through vascular access devices, e.g. central venous catheter (CVC) can contain residual heparin, which can cause incomplete clotting and consequently fibrinogen interference in serum protein electrophoresis (SPE). We hypothesized that this problem may be overcome by addition of thrombin and aimed to find a simple thrombin-based method for fibrinogen interference removal. Materials and methods: Blood samples of 51 haemodialysed patients with CVC were drawn through catheter into Clot Activator Tube (CAT) and Rapid Serum Tube Thrombin (RST) vacutainers (Becton Dickinson, New Jersey, USA) following the routine hospital protocols and analysed with gel-electrophoresis (Sebia, Lisses, France). Samples were redrawn in the CAT tubes and re-analysed after being treated with thrombin using two methods: transferring CAT serum into RST vacutainer and treatment of CAT serum with fibrinogen reagent (Multifibren U, Siemens, Marburg, Germany). Results: Direct blood collection in RST proved to be slightly more efficient than CAT in removing the interfering band in beta fraction (CAT removed 6/51 and RST removed 12/51, P = 0.031). Transferring CAT serum into the RST vacutainer proved to be more efficient for subsequent removal of interfering band from CAT serum than the addition of fibrinogen reagent (39/45 vs. 0/45 samples with efficiently removed interfering band, P &lt; 0.001). Conclusion: Fibrinogen interference caused by incomplete clotting because of residual heparin can be overcome by addition of thrombin. Transferring CAT serum into the RST vacutainer was the most efficient method.

scholarly journals Blood Collection Tubes Influence Serum Ficolin-1 and Ficolin-2 Levels

2013 ◽  
Vol 21 (1) ◽  
pp. 51-55 ◽  
Author(s):  
Allison M. Brady ◽  
Brady L. Spencer ◽  
Ann R. Falsey ◽  
Moon H. Nahm
Keyword(s):  
Lectin Pathway ◽  
Blood Collection ◽  
Sample Collection ◽  
Serum Samples ◽  
Content Type ◽  
Mannose Binding ◽  
Related Proteins ◽  
Blood Collection Tubes ◽  
Clot Activator ◽  
Binding Lectin

ABSTRACTThe ficolins are members of a recently discovered family of host innate opsonins that can activate the lectin pathway of complement. The ficolins bind many ligands, although they are typically described as binding acetylated sugars. Ficolin-1 (M-ficolin) and ficolin-2 (L-ficolin) are known to bindStreptococcus pneumoniaeserotypes 19C and 11A, respectively. While studying the binding of ficolins to pneumococci, we found variations in ficolin-2 binding among serum samples collected in different types of blood collection tubes. Plastic tubes, which contain a silica clot activator, yielded sera with reduced ficolin-2 binding and apparent ficolin-2 levels. We found that the silica clot activator eluted from plastic red-top tubes inhibited ficolin-2 ligand binding, while other related proteins, like mannose-binding lectin (MBL) and ficolin-1, were not affected. These tube types did not affect the concentrations of other related opsonins (C1q, MBL, or ficolin-3 [H-ficolin]). Interestingly, we also found that ficolin-1 levels were increased 2- to 3-fold in plastic serum separator tubes compared to the increases in other tube types. These findings have implications for future ficolin-1 and ficolin-2 studies, as proper sample collection and handling are essential.

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