Fertility restoration of racing mare with persistent corpus luteum

Background and Aim: Persistent corpus luteum (PCL) causes anestrus in mares. This study aimed to determine the effect of intrauterine prostaglandin F2α (PGF2α) treatment on PCL of racing mares to restore fertility. Materials and Methods: Twelve racing mares suspected with PCL were diagnosed using transrectal palpation and confirmed by serum progesterone (P4) concentration measurement. PGF2α was infused intrauterine, followed by serum collection at 24, 48, and 72 h after. Estrous symptoms were monitored, and mating was conducted on day 3 of estrus with an earlier injection of 8.4 μg gonadotropin-releasing hormone twice a day. Transrectal palpation was performed on days 21-30 to observe the corpus luteum. Pregnancy diagnosis was performed rectally on 40-45 days post-mating and confirmed using Doppler ultrasound scanning. Results: Eleven of the 12 mares had PCL. There was a dramatic reduction in the P4 concentration following PGF2α treatment of mares with PCL. All mares exhibited estrus 2.6±0.55 days post-treatment with a P4 concentration of 0.12±0.12 ng/mL. Rectal palpation and P4 concentration on 21-30 days after estrous onset showed that all mares were ovulating. The evaluation of P4 concentration on days 40-45 post-mating showed that all mares were still in the luteal phase. However, the pregnancy rate was only 54.5% based on rectal palpation and Doppler ultrasound scanning. Conclusion: Treatment of PCL in racing mares with an intrauterine infusion of PGF2α restored the estrous cycle and induced ovulation and pregnancy.

Download Full-text

Effect on corpus luteum function of luteal phase administration of a potent gonadotropin-releasing hormone analog (nafarelin)**Supported by National Institute of Child Health and Human Development contract N01-HD-0-2811.

Fertility and Sterility ◽

10.1016/s0015-0282(16)48610-3 ◽

1985 ◽

Vol 43 (6) ◽

pp. 844-850 ◽

Cited By ~ 10

Author(s):

Eldon D. Schriock ◽

Scott E. Monroe ◽

Mary C. Martin ◽

Milan R. Henzl ◽

Robert B. Jaffe

Keyword(s):

Child Health ◽

Corpus Luteum ◽

Human Development ◽

Luteal Phase ◽

Gonadotropin Releasing Hormone ◽

Releasing Hormone ◽

Hormone Analog

Download Full-text

CONCENTRATION OF PROSTAGLANDIN F2α AND STEROIDS IN THE HUMAN CORPUS LUTEUM

Journal of Endocrinology ◽

10.1677/joe.0.0730115 ◽

1977 ◽

Vol 73 (1) ◽

pp. 115-122 ◽

Cited By ~ 38

Author(s):

I. A. SWANSTON ◽

K. P. McNATTY ◽

D. T. BAIRD

Keyword(s):

Menstrual Cycle ◽

Corpus Luteum ◽

Luteal Phase ◽

Prostaglandin F2α ◽

Corpora Lutea ◽

Progesterone Concentration ◽

Late Luteal Phase ◽

Prostaglandin F ◽

Human Corpus Luteum

SUMMARY The concentration of prostaglandin F2α (PGF2α), progesterone, pregnenolone, oestradiol-17β, oestrone, androstenedione and testosterone was measured in corpora lutea obtained from 40 women at various stages of the menstrual cycle. The concentration of PGF2α was significantly higher in corpora lutea immediately after ovulation (26·7 ± 3·9 (s.e.m.) ng/g, P < 0·005) and in corpora albicantia (16·3 ± 3·3 ng/g, P < 0·005) than at any other time during the luteal phase. There was no correlation between the concentration of PGF2α and that of any steroid. The progesterone concentration was highest in corpora lutea just after ovulation (24·9 ± 6·7 μg/g) and in early luteal groups (25·7 ± 6·8 μg/g) but declined significantly (P < 0·05) to its lowest level in corpora albicantia (1·82 ± 0·66 μg/g). The concentration of oestradiol-17β in the corpus luteum and luteal weight were significantly greater during the mid-luteal phase than at any other stage (concentration 282 ± 43 ng/g, P < 0·05; weight 1·86 ± 0·18 g, P < 0·005). The results indicate that regression of the human corpus luteum is not caused by a rise in the ovarian concentration of PGF2α in the late luteal phase of the cycle.

Download Full-text

118 A retrospective analysis of follicular dynamics, LH, estradiol-17β, and progesterone in prostaglandin F2α-induced estrus of Beetal goats

Reproduction Fertility and Development ◽

10.1071/rdv31n1ab118 ◽

2019 ◽

Vol 31 (1) ◽

pp. 185

Author(s):

A. Murtaza ◽

M. I. R. Khan ◽

W. Ahmad ◽

T. Sohail ◽

I. Mohsin ◽

...

Keyword(s):

Single Dose ◽

Corpus Luteum ◽

Luteal Phase ◽

Linear Models ◽

Variable Interval ◽

Prostaglandin F2α ◽

Follicle Diameter ◽

Coefficient Corrélation ◽

Student’S T ◽

Follicular Dynamics

The objective of the study was to reveal the factors associated with variable interval to ovulation following a single dose of prostaglandin F2α (PGF2) in Beetal goats. In experiment 1, the extent of ovulation time was demonstrated after synchronizing Beetal goats (n=38) by using a single injection of PGF2 based on corpus luteum detection by B-mode transrectal ultrasonography. Ovulations were detected every 6h from PGF2 until ovulation. To explore the changes in follicular dynamics, endocrine profiles and behavioural events due to the variation in the interval to ovulation, another set of goats (n=13; experiment 2) were synchronized by a single dose of PGF2 given at random days of the luteal phase. Follicular dynamics were monitored every 12h from PGF2 until ovulation and oestrus was detected by apronized bucks every 6h from PGF2 treatment. Blood sampling (3mL) for oestradiol-17β and progesterone was done every 12h from PGF2 until ovulation and every 2h for LH assay by radioimmunoassay. Data were analysed by Student’s t-test, general linear models, and Pearson coefficient correlation (SPSS, version 20.0, SPSS Inc., Chicago, IL, USA). Results revealed that ovulation time varied from 60 to 96h after PGF2, and 57% of ovulations occurred by 72h after PGF2 (experiment 1). Based on this observation, goats (n=13) in second experiment were retrospectively divided into early and late ovulators (i.e. 72 and 84h following PGF2, respectively). Onset of oestrus, peak oestradiol-17β concentration, and LH surge after PGF2 were first observed in early than late ovulators (P<0.05). The goats having greater follicle diameter and smaller corpus luteum diameter at the time of PGF2 ovulated earlier than late ovulators (5.4±0.2v. 4.3±0.2mm and 10±0.6v. 11.8±0.3mm, respectively; P<0.05). Likewise, plasma progesterone concentration tended to be lower (P=0.087) in early than late ovulators. However, interval from onset of oestrus to peak LH and ovulation, duration of standing oestrus, interval from LH peak to ovulation, diameter and growth rate of preovulatory follicles, and number of ovulations were similar (P>0.05) between early and late ovulators. In conclusion, the size of dominant follicle and corpus luteum at the time of PGF2 determines the interval to ovulation following a single dose of PGF2 during the luteal phase.

Download Full-text

The role of vascularisation of the corpus luteum in the short luteal phase studied by Doppler ultrasound

Neuro-Ophthalmology ◽

10.3109/01658109209058103 ◽

1992 ◽

Vol 12 (1) ◽

pp. 321-323

Author(s):

Helena Tinkanen

Keyword(s):

Corpus Luteum ◽

Doppler Ultrasound ◽

Luteal Phase

Download Full-text

Estrus Synchronization Using Prostaglandin F2α (PGF2α) and Combination of PGF2α and Gonadotropin-Releasing Hormone In Ongole Crossbred

E3S Web of Conferences ◽

10.1051/e3sconf/202015101009 ◽

2020 ◽

Vol 151 ◽

pp. 01009

Author(s):

Pudji Astuti ◽

Claude M. Airin ◽

Slamet Widiyanto ◽

Sarmin Sarmin ◽

Amelia Hana ◽

...

Keyword(s):

Statistical Methods ◽

Artificial Insemination ◽

Luteal Phase ◽

Prostaglandin F2α ◽

Gonadotropin Releasing Hormone ◽

Estrus Synchronization ◽

Significant Difference ◽

Estrus Detection ◽

Female Cattle ◽

Estrus Phase

The response of estrus can be enhanced by providing PGF2α and a combination of PGF2αGnRH so that the time of mating using Artificial Insemination (AI) can be held precisely. This study aims to compare the response of estrus synchronization using PGF2α compare to PGF2α-GnRH. Twenty female cattle Ongole Crossbreds, 4-6 years, BCS average of 3-4 were used in this research. Cattle were selected based on their luteal phase at the days of 7 to 18 by rectal examination. Then, all of the cattle were injected by cloprostenol® with a dose of 500 ug intrauterine. Ten of the animals were injected by 2.5 mL of Fertagyl® intramuscularly after 48 hours then continued observing and response estrus detection. The onset of estrus using PGF2α was detected on 75.94 ± 0.78, while 41.57 ± 28.40 hours for PGF2α-GnRH Using statistical, there was a significant difference (P<0.05). Duration of estrus phase using PGF2α was 24.65 ± 0.49 whereas PGF2α-GnRH was 10.88 ± 7.45 hours. Using statistical methods, there was a significant difference (P <0.05). It could be concluded that the use of PGF2α was more effective for the duration and intensity of estrus, and a combination of PGF2α -GnRH is more effective for stimulating estrus.

Download Full-text

Cloning of a buffalo (Bubalus bubalis) prostaglandin F2α receptor: changes in its expression and concentration in the buffalo cow corpus luteum

Reproduction ◽

10.1530/rep.1.00107 ◽

2004 ◽

Vol 127 (6) ◽

pp. 705-715 ◽

Cited By ~ 10

Author(s):

Shalu Verma-Kumar ◽

S V Srinivas ◽

P Muraly ◽

Vijay K Yadav ◽

R Medhamurthy

Keyword(s):

Amino Acid ◽

Corpus Luteum ◽

Prostaglandin F2α ◽

Stage Iv ◽

Oestrous Cycle ◽

Full Length ◽

Stage I ◽

Stage Ii ◽

Serum Progesterone ◽

Buffalo Cow

Acting primarily through its specific G protein-coupled receptor termed FPr, prostaglandin (PG) F2α induces regression of the corpus luteum (CL) at the end of a non-fertile oestrous cycle. This study was aimed at cloning a full-length cDNA for FPr and determining its expression and protein concentrations during different stages of CL development in the water buffalo. Serum progesterone and StAR expression were determined to establish temporal relationships between indices of steroidogenesis and changes in FPr expression at different stages of CL development. In contrast to the dairy cow, the stage IV CL (day 20 of the oestrous cycle) did not appear to be functionally regressed in the buffalo. Molecular cloning of a cDNA encoding the buffalo FPr yielded a full length 2193 bp FPr cDNA containing a single open reading frame encoding a 362 amino acid protein with seven putative membrane-spanning domains. The deduced buffalo FPr amino acid sequence possesses a high degree of identity with the other mammalian homologues. Steady state concentration of buffalo FPr transcript increased (P > 0.05) from stage I to stage II/III, and declined at 18 h post PGF2α injection. The FPr concentration expressed as fmol/μg of plasma membrane protein showed an increase (P > 0.05) from stage I (1.98 ± 0.10), through stage II/III (2.42 ± 0.48) to stage IV (2.77 ± 0.18). High affinity FPr was observed in stage I (Kd 4.86 nmol) and stage II/III (Kd 6.28 nmol) while low affinity FPr (Kd 19.44 nmol) was observed in stage IV. In conclusion, we have cloned a full length FPr cDNA from buffalo cow CL and observed that FPr mRNA expression, receptor number and affinity did not vary significantly (P > 0.05) within the luteal phase of the oestrous cycle.

Download Full-text

Titrating Luteinizing Hormone Replacement to Sustain the Structure and Function of the Corpus Luteum after Gonadotropin-Releasing Hormone Antagonist Treatment in Rhesus Monkeys1

The Journal of Clinical Endocrinology & Metabolism ◽

10.1210/jcem.84.1.5362 ◽

1999 ◽

Vol 84 (1) ◽

pp. 342-349

Author(s):

Diane M. Duffy ◽

Dennis R. Stewart ◽

Richard L. Stouffer

Keyword(s):

Life Span ◽

Corpus Luteum ◽

Ribonucleic Acid ◽

Dose Regimen ◽

Luteal Phase ◽

Gnrh Antagonist ◽

Hormone Replacement ◽

Dependent Manner ◽

Serum Progesterone ◽

Messenger Ribonucleic Acid

These studies were designed to identify 1) a regimen of a third generation GnRH antagonist that abolishes primate luteal function, and 2) the amount of LH replacement required to maintain the structure and functional life span of the corpus luteum of the menstrual cycle after GnRH antagonist treatment. A single injection of antide at 3 or 5 mg/kg BW on day 6 of the luteal phase suppressed serum progesterone levels within 1 day of treatment, but levels recovered within 4 days. Administration of antide (3 mg/kg) for 3 days (luteal days 6–8) reduced (P < 0.05) serum progesterone below 1 ng/mL and maintained these low levels for the entire sampling period; in subsequent experiments, all monkeys received this antide regimen. Fixed doses (5, 10, or 20 IU) of recombinant human LH administered at 8-h intervals during and after antide treatment stimulated progesterone production in a dose-dependent manner; these monkeys menstruated earlier than controls regardless of treatment group. Replacement with an escalating dose regimen (5–20 IU) of LH resulted in typical serum progesterone and relaxin levels throughout a luteal phase of normal length. Corpora lutea removed on day 10 from monkeys treated with antide alone had decreased wet weight (P < 0.05) and few large luteal cells; coadministration of the escalating dose regimen of LH maintained luteal structure similar to that seen in time-matched controls. Antide-only treatment increased progesterone receptor (PR) messenger ribonucleic acid, but decreased PR immunostaining in luteal tissue; the escalating dose regimen of LH maintained PR messenger ribonucleic acid and immunostaining similar to those in controls. This study indicates that during GnRH antagonist administration, an escalating dose regimen of LH replacement is optimal for maintenance of the structure and functional life span of the primate corpus luteum.

Download Full-text

Aquapuncture Therapy of Repeat Breeding in Dairy Cattle

The American Journal of Chinese Medicine ◽

10.1142/s0192415x02000296 ◽

2002 ◽

Vol 30 (02n03) ◽

pp. 397-404 ◽

Cited By ~ 11

Author(s):

J. H. Lin ◽

L. S. Wu ◽

Y. L. Wu ◽

C. S. Lin ◽

N. Y. J. Yang

Keyword(s):

Glucose Solution ◽

Economic Loss ◽

Gonadotropin Releasing Hormone ◽

Hypodermic Needle ◽

Acupuncture Points ◽

Dairy Herds ◽

Progesterone Concentration ◽

Serum Progesterone ◽

Experimental Animals ◽

Rectal Palpation

Repeat breeders cause great economic loss in dairy herds. This study determined the efficacy of simple aquapuncture therapy (acupuncture combined with injection) to treat repeat breeders in two dairy herds (one in Hsinchu County and the other in Tainan County). Both herds had used gonadotropin-releasing hormone (GnRH) beforehand to treat the symptom but the success rate was poor — about 30%. Therefore, cows that failed to respond to GnRH were given the aquapuncture treatment. A total of 18 animals (two heifers and 16 cows) that had failed to conceive to 3-9 services were treated by aquapuncture, in which 10 ml and 5 ml of 50% glucose solution were injected at Baihui (Hundred Meetings, the depression in the center of the lumbosacral space) and Shenpeng (Kidney Shelf, the depression in the space between the lumbar vertebral transverse processes 5-6) acupoints, respectively with a 21G, 1.5-inch hypodermic needle. Both acupoints belong to the traditional acupuncture points of bovine and equine systems. Most animals showed heat within 14 days after aquapuncture and were inseminated artificially. Serum progesterone concentrations and rectal palpation were used for pregnancy diagnosis. The pregnancy rate after the treatment was 14/18 (77.7%) and 12/18 (66.6%) based on progesterone concentration and rectal palpation respectively, suggesting acupuncture as a simple and effective method to treat repeat breeders in dairy herds. However, the final result obtained from actual delivery of fetus in the experimental animals was only 8/18 (44.4%).

Download Full-text