scholarly journals Biosynthesis and Characterization of Bioplastic Poly-3-hydroxybutyrate from Hydrolyzate of Ulva using Bacillus subtilis rnM

2019 ◽  
Vol 31 (12) ◽  
pp. 2811-2815
Author(s):  
T. Revadhi ◽  
R. Nanthini
Keyword(s):  
Growth Conditions ◽  
Bacterial Strains ◽  
High Temperature And Pressure ◽  
Treatment Conditions ◽  
Wide Range ◽  
Nmr Techniques ◽  
Temperature And Pressure ◽  
Pre Treatment ◽  
New Generation

Biopolymers are a new generation biopolymers, which have wide range of applications. Poly-3-hydroxybutyrate (PHB) is one of the best biopolymers for replacement of non-biodegradable petroleum-based plastic and can be produced from organic wastes source by some bacterial strains under imbalance growth conditions. In present study, hydrolyzate was prepared from biomass of seaweed Ulva by acid pre-treated and used as a feedstock for PHB biosynthesis by Bacillu subtilis rnM. The pre-treatment was carried out by two different pre-treatment conditions such as room temperature and high temperature and pressure (HTP). The hydrolysate prepared by 2 % HCl at HTP supported for the maximum PHB biosynthesis than the other pre-treated conditions. The yield of PHB obtained by B. subtilis rnM when tested with laboratory grade sugars was lower to that achieved with hydrolysate of Ulva sp. The biosynthesized PHB was characterized by TGA, DSC, FTIR, XRD and NMR techniques.

scholarly journals Solid-State NMR Techniques for the Structural Characterization of Cyclic Aggregates Based on Borane–Phosphane Frustrated Lewis Pairs

Molecules ◽  
2020 ◽  
Vol 25 (6) ◽  
pp. 1400 ◽  
Author(s):  
Robert Knitsch ◽  
Melanie Brinkkötter ◽  
Thomas Wiegand ◽  
Gerald Kehr ◽  
Gerhard Erker ◽  
...  
Keyword(s):  
Solid State ◽  
Structural Characterization ◽  
Solid State Nmr ◽  
Magic Angle ◽  
Frustrated Lewis Pairs ◽  
Wide Range ◽  
Nmr Techniques ◽  
Nmr Methods ◽  
Lewis Pairs

Modern solid-state NMR techniques offer a wide range of opportunities for the structural characterization of frustrated Lewis pairs (FLPs), their aggregates, and the products of cooperative addition reactions at their two Lewis centers. This information is extremely valuable for materials that elude structural characterization by X-ray diffraction because of their nanocrystalline or amorphous character, (pseudo-)polymorphism, or other types of disordering phenomena inherent in the solid state. Aside from simple chemical shift measurements using single-pulse or cross-polarization/magic-angle spinning NMR detection techniques, the availability of advanced multidimensional and double-resonance NMR methods greatly deepened the informational content of these experiments. In particular, methods quantifying the magnetic dipole–dipole interaction strengths and indirect spin–spin interactions prove useful for the measurement of intermolecular association, connectivity, assessment of FLP–ligand distributions, and the stereochemistry of adducts. The present review illustrates several important solid-state NMR methods with some insightful applications to open questions in FLP chemistry, with a particular focus on supramolecular associates.

Raman spectroscopic characterization of CH4density over a wide range of temperature and pressure

2014 ◽  
Vol 45 (8) ◽  
pp. 696-702 ◽  
Author(s):  
Linbo Shang ◽  
I-Ming Chou ◽  
R. C. Burruss ◽  
Ruizhong Hu ◽  
Xianwu Bi
Keyword(s):  
Spectroscopic Characterization ◽  
Raman Spectroscopic ◽  
Wide Range ◽  
Temperature And Pressure

Synthesis and Characterization of the ZnO-TiO2 Nanoparticles by a Hydrothermal Processing

2006 ◽  
Vol 324-325 ◽  
pp. 1297-1300 ◽  
Author(s):  
J.W. Kim ◽  
S.W. Kim ◽  
K.S. Park ◽  
J.K Lee ◽  
Byung Ik Kim ◽  
...  
Keyword(s):  
Average Particle Size ◽  
Ammonium Hydroxide ◽  
Average Particle ◽  
Hydrothermal Processing ◽  
Zinc Titanate ◽  
High Temperature And Pressure ◽  
Metal Precursors ◽  
Temperature And Pressure ◽  
Zinc Titanates

Zinc titanate nanoparticles were prepared under high temperature and pressure conditions by precipitation from metal precursors with aqueous ammonium hydroxide. Zinc titanates powders were obtained in the temperature range of 180-230 for 4 h. The phase of synthesized particle with calcined at 800 for 2h. ZnTiO3, Zn2TiO4, Zn2Ti3O8, TiO2, ZnO. The average particle size and distribution of the synthesized zinc titanate nanoparticles were below 100 nm and narrow, respectively.

scholarly journals Characterization of the distribution of internal motions in the basic pancreatic trypsin inhibitor using a large number of internal NMR probes

1983 ◽  
Vol 16 (1) ◽  
pp. 1-57 ◽  
Author(s):  
Gerhard Wagner
Keyword(s):  
Trypsin Inhibitor ◽  
Side Chains ◽  
Internal Motions ◽  
Wide Range ◽  
Basic Pancreatic Trypsin Inhibitor ◽  
Pancreatic Trypsin Inhibitor ◽  
External Conditions ◽  
Nmr Techniques ◽  
Aromatic Side Chains

The experimental observations described in this article indicated that a distribution of many different fluctuations is present in a globular protein. These fluctuations were characterized by observation of many natural internal probes such as the labile peptide protons and the aromatic side chains. The conditions which are necessary to get reactions of the internal probes have been discussed in detail. The structural interpretation of the data was facilitated by the development and the use of new NMR techniques which provided the identification of the resonances of all the labile peptide protons. With NOE measurements a distinction between correlated and uncorrelated exchange events was obtained. This enabled us to elucidate the exchange mechanism over a wide range of p2H and temperature and to classify different subsets of fluctuations with respect to their lifetimes. It was further demonstrated that a change of external conditions such as temperature, p2H or pressure can change the distribution of fluctuations in the protein. The mechanisms responsible for rotation of internal aromatic side chains were also found to change with temperature, and mechanistic aspects of these fluctuations were discussed.

scholarly journals Characterization of a Coliphage AS1 isolated from sewage effluent in Pakistan

2022 ◽  
Vol 82 ◽  
Author(s):  
A. Ullah ◽  
T. Qamash ◽  
F. A. Khan ◽  
A. Sultan ◽  
S. Ahmad ◽  
...  
Keyword(s):  
Control Agent ◽  
Lytic Activity ◽  
Sewage Effluent ◽  
Drug Resistant ◽  
Bacterial Strains ◽  
Wide Range ◽  
Maximum Stability ◽  
Conventional Antibiotics ◽  
Bacterial Control

Abstract The emergence of multi-drug resistant (MDR) bacterial strains, which are posing a global health threat has developed the interest of scientists to use bacteriophages instead of conventional antibiotics therapy. In light of an increased interest in the use of phage as a bacterial control agent, the study aimed to isolate and characterize lytic phages from sewage effluent. During the current study, bacteriophage AS1 was isolated from sewage effluent against E.coli S2. The lytic activity of phageAS1 was limited to E.coli S2 strain showing monovalent behavior. The calculated phage titer was 3.5×109 pfu/ml. PhageAS1 was stable at a wide range of pH and temperature. The maximum stability was recorded at 37ºC and pH 7.0, while showing its normal lytic activity at temperature 60ºC and from pH 5.0 to11.0 respectively. At temperature 70ºC, phage activity was somewhat reduced whereas, further increase in temperature and decrease or increase in pH completely inactivated the phage. From the current study, it was concluded that waste water is a best source for finding bacteriophages against multi-drug resistant bacterial strains and can be used as bacterial control agent.

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