scholarly journals Characterization of a Coliphage AS1 isolated from sewage effluent in Pakistan

2022 ◽  
Vol 82 ◽  
Author(s):  
A. Ullah ◽  
T. Qamash ◽  
F. A. Khan ◽  
A. Sultan ◽  
S. Ahmad ◽  
...  
Keyword(s):  
Control Agent ◽  
Lytic Activity ◽  
Sewage Effluent ◽  
Drug Resistant ◽  
Bacterial Strains ◽  
Wide Range ◽  
Maximum Stability ◽  
Conventional Antibiotics ◽  
Bacterial Control

Abstract The emergence of multi-drug resistant (MDR) bacterial strains, which are posing a global health threat has developed the interest of scientists to use bacteriophages instead of conventional antibiotics therapy. In light of an increased interest in the use of phage as a bacterial control agent, the study aimed to isolate and characterize lytic phages from sewage effluent. During the current study, bacteriophage AS1 was isolated from sewage effluent against E.coli S2. The lytic activity of phageAS1 was limited to E.coli S2 strain showing monovalent behavior. The calculated phage titer was 3.5×109 pfu/ml. PhageAS1 was stable at a wide range of pH and temperature. The maximum stability was recorded at 37ºC and pH 7.0, while showing its normal lytic activity at temperature 60ºC and from pH 5.0 to11.0 respectively. At temperature 70ºC, phage activity was somewhat reduced whereas, further increase in temperature and decrease or increase in pH completely inactivated the phage. From the current study, it was concluded that waste water is a best source for finding bacteriophages against multi-drug resistant bacterial strains and can be used as bacterial control agent.

scholarly journals Lytic bacteriophages as a potential alternative to control Staphylococcus aureus

2019 ◽  
Vol 54 ◽  
Author(s):  
Juliana Almeida Leite ◽  
Hyago Passe Pereira ◽  
Cristiano Amâncio Vieira Borges ◽  
Bruna Rios Coelho Alves ◽  
Alessandra Isis Alves Pinheiro Ramos ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Dairy Products ◽  
Biological Control Agent ◽  
Bovine Mastitis ◽  
Restriction Enzymes ◽  
Control Agent ◽  
Lytic Activity ◽  
Wide Range ◽  
Potential Alternative ◽  
Dna Fragment

Abstract: The objective of this work was to characterize autochthonous bacteriophages and to determine their lytic activity on Staphylococcus aureus. Six phages were isolated from dairy barn flush water through enrichment cultures with three S. aureus strains. All phages were characterized by DNA digestion by restriction enzymes and sequencing of the DNA fragment encoding endolysin. Each phage was tested against 100 S. aureus strains isolated from bovine mastitis and from dairy products using the lysis-plate method. The sequences of the endolysin gene were highly conserved, with nucleotide similarity higher than 99% among the isolated phages. Three domains involved in the recognition and lysis of the bacterial cell wall were identified. Two bacteriophages isolated from the dairy barns present high lytic activity on S. aureus, on a wide range of host strains, indicating their potential for studies on phage therapy in dairy cattle or as a biological control agent for dairy products.

scholarly journals Characterization and Genome Study of Novel Lytic Bacteriophages against Prevailing Saprophytic Bacterial Microflora of Minimally Processed Plant-Based Food Products

2021 ◽  
Vol 22 (22) ◽  
pp. 12460
Author(s):  
Michał Wójcicki ◽  
Paulina Średnicka ◽  
Stanisław Błażejak ◽  
Iwona Gientka ◽  
Monika Kowalczyk ◽  
...  
Keyword(s):  
Biological Control ◽  
Genome Sequencing ◽  
Biological Control Agent ◽  
Antibiotic Resistance Genes ◽  
Food Products ◽  
Lytic Activity ◽  
Municipal Sewage ◽  
Bacterial Strains ◽  
Minimally Processed ◽  
Wide Range

The food industry is still searching for novel solutions to effectively ensure the microbiological safety of food, especially fresh and minimally processed food products. Nowadays, the use of bacteriophages as potential biological control agents in microbiological food safety and preservation is a promising strategy. The aim of the study was the isolation and comprehensive characterization of novel bacteriophages with lytic activity against saprophytic bacterial microflora of minimally processed plant-based food products, such as mixed leaf salads. From 43 phages isolated from municipal sewage, four phages, namely Enterobacter phage KKP 3263, Citrobacter phage KKP 3664, Enterobacter phage KKP 3262, and Serratia phage KKP 3264 have lytic activity against Enterobacter ludwigii KKP 3083, Citrobacter freundii KKP 3655, Enterobacter cloacae KKP 3082, and Serratia fonticola KKP 3084 bacterial strains, respectively. Transmission electron microscopy (TEM) and whole-genome sequencing (WGS) identified Enterobacter phage KKP 3263 as an Autographiviridae, and Citrobacter phage KKP 3664, Enterobacter phage KKP 3262, and Serratia phage KKP 3264 as members of the Myoviridae family. Genome sequencing revealed that these phages have linear double-stranded DNA (dsDNA) with sizes of 39,418 bp (KKP 3263), 61,608 bp (KKP 3664), 84,075 bp (KKP 3262), and 148,182 bp (KKP 3264). No antibiotic resistance genes, virulence factors, integrase, recombinase, or repressors, which are the main markers of lysogenic viruses,b were annotated in phage genomes. Serratia phage KKP 3264 showed the greatest growth inhibition of Serratia fonticola KKP 3084 strain. The use of MOI 1.0 caused an almost 5-fold decrease in the value of the specific growth rate coefficient. The phages retained their lytic activity in a wide range of temperatures (from –20 °C to 50 °C) and active acidity values (pH from 4 to 11). All phages retained at least 70% of lytic activity at 60 °C. At 80 °C, no lytic activity against tested bacterial strains was observed. Serratia phage KKP 3264 was the most resistant to chemical factors, by maintaining high lytic activity across a broader range of pH from 3 to 11. The results indicated that these phages could be a potential biological control agent against saprophytic bacterial microflora of minimally processed plant-based food products.

scholarly journals Photophysical and Bactericidal Properties of Pyridinium and Imidazolium Porphyrins for Photodynamic Antimicrobial Chemotherapy

Molecules ◽  
2021 ◽  
Vol 26 (4) ◽  
pp. 1122
Author(s):  
Florent Le Guern ◽  
Tan-Sothéa Ouk ◽  
Issabayev Yerzhan ◽  
Yesmurzayeva Nurlykyz ◽  
Philippe Arnoux ◽  
...  
Keyword(s):  
Antimicrobial Chemotherapy ◽  
Photophysical Properties ◽  
Good Alternative ◽  
Amide Bond ◽  
Drug Resistant ◽  
Bacterial Strains ◽  
Societal Issues ◽  
Photodynamic Antimicrobial Chemotherapy ◽  
Bactericidal Properties

Despite advances achieved over the last decade, infections caused by multi-drug-resistant bacterial strains are increasingly becoming important societal issues that need to be addressed. New approaches have already been developed in order to overcome this problem. Photodynamic antimicrobial chemotherapy (PACT) could provide an alternative to fight infectious bacteria. Many studies have highlighted the value of cationic photosensitizers in order to improve this approach. This study reports the synthesis and the characterization of cationic porphyrins derived from methylimidazolium and phenylimidazolium porphyrins, along with a comparison of their photophysical properties with the well-known N-methylpyridyl (pyridinium) porphyrin family. PACT tests conducted with the tetracationic porphyrins of these three families showed that these new photosensitizers may offer a good alternative to the classical pyridinium porphyrins, especially against S.aureus and E.coli. In addition, they pave the way to new cationic photosensitizers by the means of derivatization through amide bond formation.

scholarly journals Biosynthesis and Characterization of Bioplastic Poly-3-hydroxybutyrate from Hydrolyzate of Ulva using Bacillus subtilis rnM

2019 ◽  
Vol 31 (12) ◽  
pp. 2811-2815
Author(s):  
T. Revadhi ◽  
R. Nanthini
Keyword(s):  
Growth Conditions ◽  
Bacterial Strains ◽  
High Temperature And Pressure ◽  
Treatment Conditions ◽  
Wide Range ◽  
Nmr Techniques ◽  
Temperature And Pressure ◽  
Pre Treatment ◽  
New Generation

Biopolymers are a new generation biopolymers, which have wide range of applications. Poly-3-hydroxybutyrate (PHB) is one of the best biopolymers for replacement of non-biodegradable petroleum-based plastic and can be produced from organic wastes source by some bacterial strains under imbalance growth conditions. In present study, hydrolyzate was prepared from biomass of seaweed Ulva by acid pre-treated and used as a feedstock for PHB biosynthesis by Bacillu subtilis rnM. The pre-treatment was carried out by two different pre-treatment conditions such as room temperature and high temperature and pressure (HTP). The hydrolysate prepared by 2 % HCl at HTP supported for the maximum PHB biosynthesis than the other pre-treated conditions. The yield of PHB obtained by B. subtilis rnM when tested with laboratory grade sugars was lower to that achieved with hydrolysate of Ulva sp. The biosynthesized PHB was characterized by TGA, DSC, FTIR, XRD and NMR techniques.

scholarly journals THE IDENTIFICATION, PRODUCTION AND CHARACTERIZATION OF NATTOKINASE, BACTERIOCIN FROM BACTERIAL SPECIES

2019 ◽  
Vol 2 (4) ◽  
pp. 91
Author(s):  
Lal Krishna
Keyword(s):  
Enzyme Activity ◽  
Bacillus Subtilis ◽  
Pathogenic Bacteria ◽  
Blood Clot ◽  
Bacterial Species ◽  
Antagonistic Activity ◽  
Bacterial Strains ◽  
Wide Range

The study was aimed at identification, production and characterization of nattokinase, bacteriocin from bacterial species. Nattokinase and bacteriocins finds a wide range of applications in Pharmaceutical industry, health care and medicine. Nattokinase is a highly active fibrinolytic enzyme secreted by Bacillus subtilis and bacteriocins are proteinaceous toxins produced by Lactobacillus to inhibit the growth of closely related bacterial strains. Bacillus subtilis and Lactobacillus isolates shown positive results to microscopic, biochemical analysis.  The nattokinase and bacteriocins were produced by optimizing the media. The enzymes were purified by ammonium sulfate precipitation and HPLC. The enzyme activity for nattokinase was found at 7 mg/ml, pH 8.0 and temperature 48 ºC and the enzyme activity for bacteriocin was found at 3.9 mg/ml, pH 6.5 and temperature 30 °C. Bacteriocins from Lactobacillus showed good antagonistic activity against pathogenic bacteria. Nattokinase from Bacillus subtilis played a significant role in thrombolytic and anti-coagulation at in vitro. The results indicated that the pure enzyme has a potential in dissolving blood clot.

scholarly journals Exploring the Potential of Calotropis Procera Ait. (Asclepiadaceae) Phytocompounds against S. Aureus TyrRS

2020 ◽  
Vol 5 (3) ◽  
pp. 1-11
Author(s):  
Athar F
Keyword(s):  
Trna Synthetase ◽  
Biologically Active ◽  
Calotropis Procera ◽  
Bacterial Strains ◽  
Protein Activity ◽  
Hydrogen Bond Interaction ◽  
Synthetic Drugs ◽  
Tropical Plant ◽  
Wide Range ◽  
Conventional Antibiotics

In medicinal chemistry the plant-based drugs as an alternative medicine is increasing day by day with realization of toxic effects and health hazards associated with the multiple uses of antibiotics and synthetic drugs and due to the growing resistance of pathogens to conventional antibiotics. Calotropis procera is a tropical plant distributed widely in Asia, Africa, and America and native to north Africa. It can produce a wide range of chemical compounds which are biologically active against multidrug resistance bacterial strains (ESKAPE). In this manuscript the docking analysis find out the antimicrobial potential of these plant-derived compounds against S. aureus tyrosyl-tRNA synthetase (PDB ID: 1JIJ). S. aureus tyrosyl-tRNA synthetase plays an essential role in protein synthesis by producing charged tRNAs. In this screening of docking score 83 phytocompounds selected compounds which has good binding affinity above -10 which Calotroproceryl acetate A (-10.1), L-rhamnose (-10.2) and Lupeol (-10.4). The interacting analysis showing all the three compounds, Lupeol has highest binding energy which has maximum hydrogen bond interaction with CYS37, HIS47, GLY49, HIS50, THR75, GLN174, ASP177 and GLN190. These residues are important for protein activity and therefore binding at these residues may hamper protein’s activity all the three compounds interacted with active site residue of S. aureus tyrosyl-tRNA synthetase and therefore it is hypothesized that these compounds are the putative target of the protein activity which enhance bacterial pathogenesis and survival.

scholarly journals Evaluation of Seaweed Sulfated Polysaccharides As Natural Antagonists Targeting Salmonella typhi OmpF: Molecular Docking and Pharmacokinetic Profiling

2021 ◽  
Author(s):  
ARUN KUMAR MALAISAMY ◽  
Mahalakshmi Murugan ◽  
Ashok Kumar Vairamuthu ◽  
Manikka Kubendran Aravind ◽  
Sathaiah Gunaseelan ◽  
...  
Keyword(s):  
Molecular Docking ◽  
Pathogenic Bacteria ◽  
Biological Activities ◽  
Salmonella Typhi ◽  
Sulfated Polysaccharides ◽  
Drug Resistant ◽  
Bacterial Strains ◽  
Microbial Diseases ◽  
Wide Range ◽  
Docking Approach

Abstract Background: Salmonella belongs to the Enterobacteriaceae family, a gram-negative, non-spore-forming, rod-shaped, motile, and pathogenic bacteria that transmit through unhygienic conditions. It is estimated that 21 million new infections arise every year, resulting in approximately 200,000 deaths. It is more prevalent among children, the old aged, and immunocompromised individuals. The frequent usage of classical antimicrobials has begun the increasing emergence of various drug-resistant pathogenic bacterial strains. Hence, this study was intended to evaluate the bioactive seaweed sulfated polysaccharides (SSP) against the ompF (outer membrane porin F) protein target of Salmonella typhi. SSP are sulfated compounds with a wide range of biological activities, such as anti-microbial, anti-allergy, anti-cancer, anti-coagulant, anti-inflammation, anti-oxidant, and anti-viral. Results: In this study, eleven compounds were targeted against S. typhi OmpF by molecular docking approach and were compared with two commercially available typhoid medications. The SSP showed good binding affinity compared to commercial drugs, particularly carrageenan/MIV-150, carrageenan lambda, fucoidan, and 3-Phenyllactate, ranked as top antagonists against OmpF. Further, pharmacokinetics and toxicology (ADMET) studies corroborated that SSP possessed drug-likeness and highly progressed in all parameters.Conclusion: AutoDockTools and Schrodinger's QikProp module results suggest that SSP could be a promising drug for extensively drug-resistant (XDR) S. typhi. To our best of knowledge, this is the first report on in-silico analysis of SSP against S. typhi OmpF. Thus, implying the capabilities of SSP's especially compounds like carrageenans, as a potential anti-microbial agent against Salmonella typhi infections. Eventually, advanced studies could corroborate SSPs to the next level of application in the crisis of XDR microbial diseases.

scholarly journals Evaluation of seaweed sulfated polysaccharides as natural antagonists targeting Salmonella typhi OmpF: molecular docking and pharmacokinetic profiling

2022 ◽  
Vol 11 (1) ◽  
Author(s):  
Malaisamy Arunkumar ◽  
Murugan Mahalakshmi ◽  
Vairamuthu Ashokkumar ◽  
Manikka Kubendran Aravind ◽  
Sathaiah Gunaseelan ◽  
...  
Keyword(s):  
Molecular Docking ◽  
Pathogenic Bacteria ◽  
Biological Activities ◽  
Salmonella Typhi ◽  
Sulfated Polysaccharides ◽  
Drug Resistant ◽  
Bacterial Strains ◽  
Microbial Diseases ◽  
Wide Range ◽  
Docking Approach

Abstract Background Salmonella belongs to the Enterobacteriaceae family, a gram-negative, non-spore-forming, rod-shaped, motile, and pathogenic bacteria that transmit through unhygienic conditions. It is estimated that 21 million new infections arise every year, resulting in approximately 200,000 deaths. It is more prevalent among children, the old aged, and immunocompromised individuals. The frequent usage of classical antimicrobials has begun the increasing emergence of various drug-resistant pathogenic bacterial strains. Hence, this study was intended to evaluate the bioactive seaweed sulfated polysaccharides (SSPs) against the ompF (outer membrane porin F) protein target of Salmonella typhi. SSP is the sulfated compound with a wide range of biological activities, such as anti-microbial, anti-allergy, anti-cancer, anti-coagulant, anti-inflammation, anti-oxidant, and anti-viral. Results In this study, eleven compounds were targeted against S. typhi OmpF by the molecular docking approach and were compared with two commercially available typhoid medications. The SSP showed good binding affinity compared to commercial drugs, particularly carrageenan/MIV-150, carrageenan lambda, fucoidan, and 3-phenyllactate, ranked as top antagonists against OmpF. Further, pharmacokinetics and toxicology (ADMET) studies corroborated that SSP possessed drug-likeness and highly progressed in all parameters. Conclusions AutoDockTools and Schrodinger's QikProp module results suggest that SSP could be a promising drug for extensively drug-resistant (XDR) S. typhi. To the best of our knowledge, this is the first report on in silico analysis of SSP against S. typhi OmpF, thus implying the capabilities of SSPs especially compounds like carrageenans, as a potential anti-microbial agent against Salmonella typhi infections. Eventually, advanced studies could corroborate SSPs to the next level of application in the crisis of XDR microbial diseases. Graphical Abstract

scholarly journals The effect of acidic pH on the interaction and lytic activity of MP1 and its H-MP1 analog in anionic lipid membrane: a biophysical study by Molecular Dynamics and Spectroscopy

2020 ◽  
Author(s):  
Ingrid Bernardes Santana Martins ◽  
Taisa Giordano Viegas ◽  
Alexandre Suman de Araujo ◽  
Joao Ruggiero Neto ◽  
Bibiana Monson de Souza ◽  
...  
Keyword(s):  
Lipid Bilayers ◽  
Lipid Membrane ◽  
Lytic Activity ◽  
Synthetic Analog ◽  
Bacterial Strains ◽  
Solution Ph ◽  
Dynamic Simulations ◽  
Gram Negative ◽  
Biophysical Study ◽  
Conventional Antibiotics

Antimicrobial peptides (AMPs) are part of the innate immune system of many species and are compounds with potential application against the development of resistant bacterial strains promoted by conventional antibiotics. The AMPs are rich in cationic and hydrophobic residues and act directly on the lipidic phase of the cell membranes. The MP1 has a broad-spectrum bactericide activity in both Gram-negative and positive bacteria, not being hemolytic or cytotoxic. H-MP1 is a synthetic analog of MP1 with lysines replaced by histidines so that its net charge could be responsive to changes in solution pH. In the present work, we investigated the effect of the solution pH on the structural properties, in the adsorption and insertion, and on the lytic activity of these peptides in lipid bilayers mimicking the cell membrane of Gram-negative bacteria, using experimental and computational biophysical techniques. The results indicate that the lytic activity of H-MP1 is sensitive to pH, increasing to an acidic environment, matching that of MP1, which is not influenced by solution pH. Molecular Dynamic simulations indicated that the adsorption process of both peptides started by the interaction of the N-terminus with the bilayer, followed by the complete adsorption of the peptide laying parallel to the bilayer plane, inducing an increase in the peptide's helical content enhancing peptides contact with the bilayer hydrophobic phase.

Extraction and characterization of sea anemones compound and its Anti bacterial and hemolytic studies

2020 ◽  
Vol 10 (4) ◽  
pp. 93-97
Author(s):  
Anil Kumar A ◽  
Raja Sheker K ◽  
Naveen B ◽  
Abhilash G ◽  
Akila CR
Keyword(s):  
Antibacterial Activity ◽  
Focal Point ◽  
Haemolytic Activity ◽  
Klebsiella Pneumonia ◽  
Bacterial Strains ◽  
Sea Anemones ◽  
Marine Plants ◽  
Heteractis Magnifica ◽  
Trididemnum Solidum

Seas assets that give us a variety of characteristic items to control bacterial, contagious and viral ailment and mostly utilized for malignancy chemotherapy practically from spineless creatures, for example, bryozoans, wipes, delicate corals, coelenterates, ocean fans, ocean bunnies, molluscs and echinoderms. In the previous 30 - 40 years, marine plants and creatures have been the focal point of overall endeavours to characterize the regular results of the marine condition. Numerous marine characteristic items have been effectively exceptional to the last phases of clinical preliminaries, including dolastatin-10, a group of peptides disengaged from Indian ocean rabbit, Dollabella auricularia. Ecteinascidin-743 from mangrove tunicate Ecteinascidia turbinata, Didemnins was isolated from Caribbean tunicate Trididemnum solidum and Conopeptides from cone snails (Conus sp.), and a developing number of up-and-comers have been chosen as promising leads for expanded pre-clinical appraisals. Sea anemones possess numerous tentacles containing stinging cells or cnidocytes. The stinging cells are equipped with small organelles known as nematocysts. The two species of sea anemones namely, Heteractis magnificaandStichodactyla haddoni, were collected from Mandapam coastal waters of Ramanathapuram district, Tamilnadu, India. The Nematocyst was collected and centrifuged, and the supernatant was lyophilized and stored for further analysis. The amount of protein from Heteractis Magnifica and Stichodactyla haddoni was estimated. The crude extract has shown haemolytic activity on chicken blood and goat blood. In the antibacterial activity of the sea anemone against six bacterial strains Staphylococcus aureus, Salmonella typhii, Salmonella paratyphii, Klebsiella pneumonia, Vibrio cholerae, Pseudomonas aeruginosa. Antibacterial activity of H. Magnifica and S.haddoni was measured as the radius of the zone of inhibition.

Sign in / Sign up

Export Citation Format

Share Document