scholarly journals Chemical Composition Analysis and Evaluation of Antibacterial Activities of Essential Oil from Clove (Syzygium aromaticum) Buds

2020 ◽  
Vol 32 (7) ◽  
pp. 1649-1652
Author(s):  
Tran Quoc Toan ◽  
Cam Thi Inh ◽  
Hoang Thi Bich ◽  
Pham Quoc Long ◽  
Tran Thi Kim Ngan ◽  
...  

Essential oils from Syzygium aromaticum (Myrtaceae) were extracted through hydrodistillation method, which applied in different fields such as relieve pain, promote healing, and aromatherapy industry. In this study, essential oil of clove buds was obtained using hydrodistillation method and evaluated for chemical compositions and physico-chemical characteristics. The averaged physico-chemical parameters were determined as specific gravity (1.0203 g/cm3), acid index (21.984), ester index (34.92). Moreover, the hydrodistillation process performance achieved by 6.85%. The composition of essential oils was analyzed by GC-MS, revealing a total of 15 volatile constituents existing in the Syzygium aromaticum oil. The major components were eugenol (76.542%), caryophyllene (4.319%), α-caryophyllene (0.456%), acete-ugenol (18.11%), caryophyllene oxide (0.192%), 4,4-dimethyl tetracyclo[6.3.2.0.(1,8)]tridecan-9-ol (0.12%). Syzygium aromaticum essential oil in this study was found to inhibit five bacteria strains including Bacillus cereus, Staphylococcus aureus, Escherichia coli, Salmonella typhimurium and Pseudomonas aeruginosa. The highest antimicrobial activity of clove essential oil was found for Bacillus cereus strain (14 mm).

2017 ◽  
Vol 2017 ◽  
pp. 1-9 ◽  
Author(s):  
Nidal Jaradat ◽  
Lina Adwan ◽  
Shadi K’aibni ◽  
Abdel Naser Zaid ◽  
Munqez J. Y. Shtaya ◽  
...  

Introduction. Interest in essential oils was recently revived with their popularity increasing in medicine, pharmacy, and aromatherapy. This study was performed to identify the chemical compositions of the essential oil of Ruta chalepensis growing wildly in three regions in Palestine and to assess and compare their antimicrobial and antioxidant activities. Methods. Identification of the essential oil was performed by gas chromatography coupled with mass spectrometry (GC-MS). Antimicrobial activity was tested against Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Methicillin-Resistant Staphylococcus aureus, and Candida albicans by using minimum inhibitory concentration (MIC) assay, while antioxidant activity was analyzed by using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging method. Results. The essential oils of R. chalepensis from Jerusalem and Hebron regions have almost identical components; the major compounds identified were linalyl acetate and β-linalool; these essential oils exerted potential antioxidant and antibacterial activities. On the other hand, the major components of the plant essential oil from Jenin region were 2-undecanone and 2-nonanone, which exhibited potential antifungal activity. Conclusions. The phytoconstituents and antioxidant and antimicrobial properties of the essential oil of R. chalepensis from different regions in Palestine were established in this study. The obtained results indicate possible applications for R. chalepensis in the treatment of various infectious and noninfectious diseases.


2017 ◽  
Vol 4 (7) ◽  
pp. 35-44
Author(s):  
T. M. Obuotor ◽  
W. O. Anibaba ◽  
O. A. Sarumi ◽  
O. P. Adeniyi

The antibacterial activities of the clove essential oil (C), turmeric essential oil (T) as well as clove and turmeric (CT) essential oil were analysed against some bacterial pathogens implicated in periodontal diseases using agar well diffusion. The bacteria include Klebsiella oxytoca, Bacillus subtilis, Pseudomonas flourescens, Pseudomonas aeruginosa, Klebsiella oxytoca, Enterobacter sp., Proteus mirabilis and Escherichia coli. At 15% concentration of the essential oils, both clove essential oil and the essential oil of clove and turmeric (CT) exhibited pronounced and varying degrees of growth inhibition zones against the bacteria (8.3+/-0.33-22.6+/-1.53 and 7.3+/-0.33-22.3+/-1.45). The MIC value for clove essential oil, tumeric essential oil and clove and turmeric (CT) essential oils ranges from 0.9-7.5%, respectively. The result shows that in general, clove essential oil has significantly greater zone of inhibition (mean) than tumeric essential oil. The bactericidal rate of clove essential oil against Klebsiella oxytoca and Bacillus subtilis was also determined. From our study, we can conclude that clove essential oil has more prevailing and sustainable antibacterial properties than turmeric essential oil even at a considerable low percentage. We recommend that clove essential oil not only has very promising potential for a broad-spectrum antibiotic drug against periodontal pathogenic bacteria. In addition, it can be used as an effective source of natural herbal antibiotics.


2019 ◽  
Vol 31 (10) ◽  
pp. 2284-2286 ◽  
Author(s):  
Tan Phat Dao ◽  
Thien Hien Tran ◽  
Phu Thuong Nhan Nguyen Nhan Nguyen ◽  
Tran Thi Kim Ngan ◽  
Ngo Thi Cam Quyen ◽  
...  

Chemical compounds from essential oils have been receiving a great deal of public attention owing to a myriad of functions, including their role as a substitute for artificial preservatives. This study reports the physico-chemical characteristics and the antibacterial activities of the essential oil isolated from the leaves of lemon (Citrus aurantifolia L.) grown in Tien Giang Province, Vietnam. The essential oils were obtained by microwave assisted hydro-distillation and their chemical composition was investigated by GC-MS. The result revealed that the oil is extremely rich in α-citral, reaching the content of 27.982 %, followed by β-citronellol and D-limonene at 20.06 and 15.732 %, respectively. Besides, antibacterial activities of extracted essential oil against 4 bacterial cultures i.e., Pseudomonas aeruginosa, Escherichia coli, Staphylococcus aureus and Bacillus cereus was examined, highlighting strong antibacterial properties of citral in the oil.


2014 ◽  
Vol 9 (12) ◽  
pp. 1934578X1400901 ◽  
Author(s):  
Acharavadee Pansanit ◽  
Patcharee Pripdeevech

The present work reports the chemical compositions, antibacterial and antioxidant activities of essential oils from T. jasminoides flowers collected from two different geographical areas, Chiang Rai and Chiang Mai, Thailand. The essential oil of T. jasminoides from the Chiang Rai area had 99 compounds representing 97.9% of the total oil composition, with E-nerolidol and α-phellandrene as the major constituents. In contrast, the essential oil of T. jasminoides collected from the Chiang Mai area contained 93 components representing 94.8% of the total oil, with trans-linalool oxide and citronellol as the major compounds. Flower oils of T. jasminoides exhibited greater antibacterial activities against Gram-negative bacteria than Gram-positive bacteria. Both oils displayed antioxidant activities.


Author(s):  
Imane Rihab Mami ◽  
Noria Merad-Boussalah ◽  
Mohammed El Amine Dib ◽  
Boufeldja Tabti ◽  
Jean Costa ◽  
...  

Aim and Objective: Oxidative stress is implicated in the development and progression of many disease. Some of appropriate actions that could be initiated to taken to resolve the problem of these diseases are search for new antioxidant substances isolated from plants. The aims of this study were to study the intraspecies variations of A. verticillata and C. caeruleus essential oils from 8 locations using statistical analysis, the in vitro antioxidant properties of collective essential oils and in combinations. Materials and Methods: The essential oils were analyzed by GC and GC-MS. The intraspecies variations of the essential oil compositions were discussed using principal component analysis (PCA) and cluster analysis (CA). The antioxidant properties were evaluated DPPH-radical scavenging activity and β-carotene bleaching test. Results: The main components of Ammoides verticillata collective essential oil (Coll EO) were thymol (30.5%), carvacrol (23.2%), p-cymene (13.1%), limonene (12.5%) and terpinene-4-ol (12.3%). While roots of Carthamus caeruleus essential oil were dominated by carline oxide (86.2%). The chemical variability allowed the discrimination of two main Groups for both Coll EOs. A direct correlation between the altitudes, climate and the chemical compositions was evidenced. Ammoides verticulata and Carthamus caeruleus Coll Eos showed good antioxidant activity. In binary mixture, the interaction both Coll Eos and between oils rich of thymol and/or carvacrol with carlina oxide produced the best synergistic effects, compared to individual essential oils and the synthetic antioxidant (BHT). Conclusion: Ammoides verticillata and Carthamus caeruleus essential oil blends can be used as a natural food preservative and alternative to chemical antioxidants.


Molecules ◽  
2020 ◽  
Vol 25 (6) ◽  
pp. 1303 ◽  
Author(s):  
Do N. Dai ◽  
Nguyen T. Chung ◽  
Le T. Huong ◽  
Nguyen H. Hung ◽  
Dao T.M. Chau ◽  
...  

Members of the genus Cinnamomum (Lauraceae) have aromatic volatiles in their leaves and bark and some species are commercially important herbs and spices. In this work, the essential oils from five species of Cinnamomum (C. damhaensis, C. longipetiolatum, C. ovatum, C. polyadelphum and C. tonkinense) growing wild in north central Vietnam were obtained by hydrodistillation, analyzed by gas chromatography and screened for antimicrobial and mosquito larvicidal activity. The leaf essential oil of C. tonkinense, rich in β-phellandrene (23.1%) and linalool (32.2%), showed excellent antimicrobial activity (MIC of 32 μg/mL against Enterococcus faecalis and Candida albicans) and larvicidal activity (24 h LC50 of 17.4 μg/mL on Aedes aegypti and 14.1 μg/mL against Culex quinquefasciatus). Cinnamomum polyadelphum leaf essential oil also showed notable antimicrobial activity against Gram-positive bacteria and mosquito larvicidal activity, attributable to relatively high concentrations of neral (11.7%) and geranial (16.6%). Thus, members of the genus Cinnamomum from Vietnam have shown promise as antimicrobial agents and as potential vector control agents for mosquitoes.


2011 ◽  
Vol 1 (6) ◽  
pp. 248-254
Author(s):  
R. Amudan ◽  
D. V. Kamat ◽  
S. D. Kamat

The essential oil of clove ( Syzygium aromaticum) holds an important positionamongst widely used essential oils. A typical steam distillation processfor the extraction of clove oil provides a 10.1% yield. Recent studies involvedthe use of enzymes such as pectinase, amylase, lignocellulase, and cellulaseon the powder of clove buds, prior to extraction. The traditional methods ofphysical and chemical extraction are effective but may affect the structure,quality and yield of the phytochemicals extracted. In the current study,hence, enzymes specific for action on the cell wall have been used in the pretreatment prior to extraction, to enhance the quality and yield of the phytochemicalsextracted. The results indicated that all the enzymes, gave morethan 50% higher yield than control in terms of weight of extracted essentialoil. A mixture of the enzymes gave the highest yield of 17.82%. Gas chromatographyresults indicated that the essential oil extracted using amylase hada maximum eugenol content of 70%, in comparison with the eugenol content(62–68%) in the essential oils extracted using the rest of the enzymes.Antibacterial activity of all the extracts was studied on methicillin â€resistantStaphylococcus aureus  (MRSA). The essential oil extracted by using amylaseinhibitedMRSA, showed a zone size of 40 mm, whereas the essential oil extractedby using lignocellulase showed a zone size of 45 mm. The gas chromatogramindicated the maximum number of peaks in this extract, whichcould be producing a combined antibacterial effect on the organism. Thespecific gravity values of the essential oil extracted using lignocellulase andamylase was 1.051 and 1.062, respectively, whereas the control had a specificgravity of 1.015.


BioResources ◽  
2007 ◽  
Vol 2 (2) ◽  
pp. 265-269 ◽  
Author(s):  
M. Hakki Alma ◽  
Murat Ertaş ◽  
Siegfrie Nitz ◽  
Hubert Kollmannsberger

In this study, clove bud oil, which was cultivated in the Mediterranean region of Turkey, was provided from a private essential oil company in Turkey. Essential oil from clove (Syzygium aromaticum L.) was obtained from steam-distillation method, and its chemical composition was analyzed by GC and GC-MS. The results showed that the essential oils mainly contained about 87.00% eugenol, 8.01% eugenyl acetate and 3.56% β-Caryophyllene. The chemical composition of the Turkish clove bud oil was comparable to those of trees naturally grown in their native regions.


2014 ◽  
Vol 9 (7) ◽  
pp. 668-677 ◽  
Author(s):  
Tatjana Mihajilov-Krstev ◽  
Dragan Radnović ◽  
Dušanka Kitić ◽  
Vesna Jovanović ◽  
Violeta Mitić ◽  
...  

AbstractThe present study investigates the chemical compositions of three Satureja montana L. ssp montana essential oils and correlates chemical variability with biological activities. GC/MS analysis showed that with an increase in altitude (100–500–800 m), a higher content of linalool, terpinen-4-ol and cis-sabinene hydrate was found, while the percentage of phenolic compounds, thymol and carvacrol decreased. Antimicrobial activity of the essential oils was tested against 7 fungal and 23 bacterial strains. The essential oil characterized by the highest content of phenols and alcohols exhibited the highest antimicrobial potential. The correlation analysis showed that the major carriers of the obtained antioxidant activity are oxygenated monoterpenes. All essential oils inhibited human serum cholinesterase activity. High antimicrobial potential, together with moderate antioxidant capacity and strong inhibition of human serum cholinesterase, classifies S. montana essential oil as a natural source of compounds that can be used in the treatment of foodborne and neurological diseases, wound and other infections, as well as for general health improvement.


2007 ◽  
Vol 76 (3) ◽  
pp. 357-361 ◽  
Author(s):  
Š. Faix ◽  
Š. Juhas ◽  
Z. Faixová

The aim of this study was to determine the effects of four essential oils intake by feed, namely Origanum vulgare, Thymus vulgaris, Cinnamomum zeylanicum Ness, and Syzygium aromaticum on antioxidant status in mice in vivo. Essential oils were in the aether oleum form. They were diluted with ethanol absolute mixed with ground pelett (0.1, 0.25, 0.57 and 1% concentration) and thereafter ethanol was evaporated. SOD, GPx activities and TAS were measured in erythrocytes and plasma spectrophotometrically with Ransod, Ransel and TAS kits from RANDOX, respectively. GPX activity showed a significant increase in 0.25% and 0.1% concentration of Origani aetheroleum. The GPx activities were decreased in 1% concentration of Thymi aetheroleum and 0.57% concentration of Cinnamomi aetheroleum and 0.57% concentration of Caryophylli aetheroleum. The total antioxidant status showed a significant decrease in 1 % concentration of Origani aetheroleum and significantly increased in 0.1% concentration. The same results were found in Thymi aetheroleum. Cinnamomi aetheroleum and Caryophylli aetheroleum had not effect on total antioxidant status. SOD activities were not significantly changed after intake of essential oils. In conclusion, our results showed, that concentration of essential oil is very important for antioxidant status and also for metabolism of mice, because a high dose of essential oil has adverse effect on metabolism of mice, representated by a lower growth of the body weight. On the other hand, essential oils at lower concentrations have positive effect on antioxidant status of mice.


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