scholarly journals THE INFLUENCE OF ULTRASONIC TREATMENT ON THE EXTRACTION OF BIOLOGICALLY ACTIVE COMPOUNDS OF PLANTS OF THE FAMILY CARYOPHYLLACEAE

2018 ◽  
pp. 145-151
Author(s):  
Лариса (Larisa) Николаевна (Nikolaevna) Зибарева (Zibareva) ◽  
Елена (Elena) Сергеевна (Sergeevna) Филоненко (Filonenko)
Keyword(s):  
Secondary Metabolites ◽  
Ultrasonic Treatment ◽  
High Performance ◽  
Control Level ◽  
Extraction Process ◽  
Biologically Active Compounds ◽  
Biologically Active ◽  
Destructive Effect ◽  
Active Compounds ◽  
The Family

The influence of ultrasonic irradiation on extraction of secondary metabolites from plants of the family Caryophyllaceae was study. It is shown that while carrying out extraction and the effects of ultrasound observed acceleration of the extraction process such biologically active compounds as ecdysteroids and flavonoids. The time of extraction of ecdysteroids from the aerial part of Silene viridiflora, Silene sendtneri, Silene roemeri, colpophylla Silene, Silene frivaldszkyana and Lychnis chalcedonica decreased by 6–12 times. Found that ultrasound has a different effect on the process of extraction of compounds from different plant species. Lychnis chalcedonica, with an average level of ecdysteroids, after 60 minutes of exposure is achieved by the extraction of the ecdysteroids is equal to the control, and after 90 min, exceeding its level by 49%. From Silene frivaldszkyana - species with high content of ecdysteroids control level were achieved after 120 min of ultrasonic treatment. High-intensity ultrasound accelerates the process, reduces the volume of solvents and increases the yield of flavonoids in 28–41% in comparison with the control. The destructive effect of ultrasound on the secondary metabolites not observed, as confirmed by UV-spectra and high performance liquid chromatography.

scholarly journals Diversity of Eleutherococcus genus in respect of biologically active compounds accumulation

2014 ◽  
Vol 60 (3) ◽  
pp. 34-43 ◽  
Author(s):  
Katarzyna Bączek
Keyword(s):  
High Performance Liquid Chromatography ◽  
Phenolic Acids ◽  
High Performance ◽  
Raw Materials ◽  
Stem Bark ◽  
Biologically Active Compounds ◽  
Biologically Active ◽  
Active Compounds ◽  
Eleutheroside B

Summary Eight species of Eleutherococcus genus grown at Rogów Arboretum collection were compared in respect of biologically active compounds accumulation, i.e. eleutheroside B and E, phenolic acids and sterols. For the determination of content of these compounds in underground organs and stem bark high performance liquid chromatography was applied. The highest content of the sum of eleutherosides B and E was observed in underground organs and stem bark of E. leucorrhizus (322.0 and 300.8 mg × 100 g-1, respectively) and E. nodiflorus (218.9 and 363.5 mg × 100g-1, respectively). In the raw materials from E. senticosus, the content of these compounds was significantly lower (177.4 and 159.3 mg × 100 g-1, respectively). E divaricatus and E. setchuenensis were characterized by the lowest accumulation of these compounds in underground organs whereas E. divaricatus, E. sessiliflorus and E. giraldii - in stem bark. Four phenolic acids were identified in the investigated species, namely: chlorogenic, rosmarinic, ferulic and caffeic acids. The main phenolic acid in the analyzed species was chlorogenic acid. The content of this compound in underground organs varied from 102.1 (E. henryi) to 958.7 mg × 100 g-1 (E. leucorrhizus) and in stem bark from 26.7 (E. giraldii) to 542.5 mg × 100 g-1 (E. setchuenensis). The content of identified sterol compounds (sitosterol 3-O-β-D-glucoside /eleutheroside A / campesterol, stigmasterol) was relatively low, but higher in underground organs in comparison with stem bark.

scholarly journals A new perspective on fungal metabolites: identification of bioactive compounds from fungi using zebrafish embryogenesis as read-out

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Jelmer Hoeksma ◽  
Tim Misset ◽  
Christie Wever ◽  
Johan Kemmink ◽  
John Kruijtzer ◽  
...  
Keyword(s):  
Biological Activity ◽  
Secondary Metabolites ◽  
High Resolution Mass Spectrometry ◽  
Biologically Active Compounds ◽  
Biologically Active ◽  
Therapeutic Drug ◽  
Preparative Hplc ◽  
Active Compounds ◽  
Vis Spectroscopy ◽  
Therapeutic Compounds

AbstractThere is a constant need for new therapeutic compounds. Fungi have proven to be an excellent, but underexplored source for biologically active compounds with therapeutic potential. Here, we combine mycology, embryology and chemistry by testing secondary metabolites from more than 10,000 species of fungi for biological activity using developing zebrafish (Danio rerio) embryos. Zebrafish development is an excellent model for high-throughput screening. Development is rapid, multiple cell types are assessed simultaneously and embryos are available in high numbers. We found that 1,526 fungal strains produced secondary metabolites with biological activity in the zebrafish bioassay. The active compounds from 39 selected fungi were purified by liquid-liquid extraction and preparative HPLC. 34 compounds were identified by a combination of chemical analyses, including LCMS, UV-Vis spectroscopy/ spectrophotometry, high resolution mass spectrometry and NMR. Our results demonstrate that fungi express a wide variety of biologically active compounds, consisting of both known therapeutic compounds as well as relatively unexplored compounds. Understanding their biological activity in zebrafish may provide insight into underlying biological processes as well as mode of action. Together, this information may provide the first step towards lead compound development for therapeutic drug development.

scholarly journals Prospects for the Production of Aromatized Wines Enriched with Biologically Active Compounds

2021 ◽  
pp. 11-28
Author(s):  
Marine Demetrashvili ◽  
◽  
Mariam Khomasuridze ◽  
Guram Tkemaladze ◽  
◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
High Performance ◽  
Biologically Active Compounds ◽  
Biologically Active ◽  
Total Phenols ◽  
Active Compounds ◽  
The Rich ◽  
Rich Content ◽  
Positive Effect ◽  
Control Samples

Within the experiment, for preparation of the aromatized wines, enriched with biologically active compounds, were used the endemic medicinal plants and fruits. In the control and trial samples, by application of high performance liquid chromatography, were determined the content of total phenols, resveratrol, quercetin, myricetin and micronutrients. Compared to the corresponding control samples – sweet fortified wine, Saperavi and Hawthorn Red Aromatized Wine contents 21%, and Rkatsiteli, Kiwi and Elecampane White Aromatized Wine, 18.25% more total phenols. Based on conducted analyses, the trial samples contain twice more antioxidants, than they are typically identified in wines: Saperavi, Blackberry, Plum and Leonurus Red Aromatized Wine is characterized with the rich content of resveratrol, while, Rkatsiteli, buckthorn and balm with - quercetin and myricetin. These compounds are widely used in pharmaceutical industry. The enrichment of wines with these antioxidant is achieved by the usage of plants and herbs. Besides, the selected materials influenced on micronutrients, their content is also significantly increased. The obtained results confirm, that the selected materials and worked out recipes are effective for the production of beverages that have a positive effect on the human health.

scholarly journals Skeletocutins M–Q: biologically active compounds from the fruiting bodies of the basidiomycete Skeletocutis sp. collected in Africa

2019 ◽  
Vol 15 ◽  
pp. 2782-2789 ◽  
Author(s):  
Tian Cheng ◽  
Clara Chepkirui ◽  
Cony Decock ◽  
Josphat C Matasyoh ◽  
Marc Stadler
Keyword(s):  
Staphylococcus Aureus ◽  
Secondary Metabolites ◽  
Biofilm Formation ◽  
Biologically Active Compounds ◽  
Biologically Active ◽  
Fruiting Bodies ◽  
Inhibition Assay ◽  
Active Compounds ◽  
New Compounds ◽  
New Metabolites

During the course of screening for new metabolites from basidiomycetes, we isolated and characterized five previously undescribed secondary metabolites, skeletocutins M–Q (1–5), along with the known metabolite tyromycin A (6) from the fruiting bodies of the polypore Skeletocutis sp. The new compounds did not exhibit any antimicrobial, cytotoxic, or nematicidal activities. However, compound 3 moderately inhibited the biofilm formation of Staphylococcus aureus (S. aureus), while compounds 3 and 4 performed moderately in the ʟ-leucine-7-amido-4-methylcoumarin (ʟ-Leu-AMC) inhibition assay. These compounds represent the first secondary metabolites reported to occur in the fruiting bodies by Skeletocutis. Interestingly, tyromycin A (6) was found to be the only common metabolite in fruiting bodies and mycelial cultures of the fungus, and none of the recently reported skeletocutins from the culture of the same strain were detected in the basidiomes.

scholarly journals Production of triterpenoids with cell and tissue cultures.

1970 ◽  
Vol 60 (4) ◽  
Author(s):  
Magdalena Malinowska ◽  
Elżbieta Sikora ◽  
Jan Ogonowski
Keyword(s):  
Higher Plants ◽  
Extraction Process ◽  
Biologically Active Compounds ◽  
Biologically Active ◽  
Therapeutic Effects ◽  
Enzymatic Reactions ◽  
Tissue Cultures ◽  
Active Compounds ◽  
Microbial Strains

Triterpenes are group of biologically active compounds which can be found in higher plants. Their main source are plants bark, leaves, twigs, fruits, resins or oils. The biological activity of triterpens is very diversified and many studies have already confirmed the following therapeutic effects: anti-inflammatory, antimicrobial, antiviral, antifungial, immunomodulatory, and hepatoprotective. Synthesis of triterpenes derivates can be performed by chemical or enzymatic reactions, however biotransformation is more specific and eliminates the side products and the molecule alterations. These processes use isolated enzymes or microorganisms. Cell culture in vitro eliminates problems like extract variability as well as instability of the compounds being obtained during the extraction process. What is more, it ensures high reproducibility and optimal regio- and enantioselectivity. The most widely used technique is a classical screening of a series of microbial strains. Studies on triterpene biotransformation give a lot of information about new biologically active compounds and let predict the metabolism of biological compounds. This review presents most important advancements in the metabolic engineering of microorganisms for the production of triterpenoids. Moreover, the review highlights general strategies to obtain rich biochemical diversity of plants by employing the biocatalysts produced by microorganisms or tissue cultures.

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