scholarly journals To Estimate the Importance of Wharton’s Jelly in the Growth of the Foetus – A Light Microscopic Study

2021 ◽  
Vol 10 (35) ◽  
pp. 3024-3029
Author(s):  
Sreekumar Rajasekharan ◽  
UmesanKannanvilakom Govindapillai ◽  
Manju Madhavan C. ◽  
Suja R. S. ◽  
Swapna T ◽  
...  
Keyword(s):  
Umbilical Cord ◽  
Wall Thickness ◽  
Umbilical Artery ◽  
Umbilical Vein ◽  
Wharton’S Jelly ◽  
External Diameter ◽  
Single Umbilical Artery ◽  
Wharton's Jelly ◽  
Significant Difference ◽  
The Mean

BACKGROUND Human umbilical cord contains two arteries and one vein with their tunica intima and tunica media layers. The role of tunica adventitia is fulfilled by Wharton’s jelly, a mucoid connective tissue. The function of Wharton’s jelly is to prevent the vessels from compression and torsion which is essential for foetal development. The purpose of the study was to estimate the importance of Wharton’s jelly in the growth of the foetus. METHODS Umbilical cord tissue collected from each case was immediately put in 10 % formalin for fixation. Slides were then stained with Haematoxylin and Eosin. These slides were then read under light microscopy and measurements were taken using a photomicrograph. Wharton’s jelly area was calculated by subtracting the total vessel area from the umbilical cord area. RESULTS The histological measurements of umbilical vessels include the external diameter, lumen diameter, wall thickness, thickness of tunica intima and tunica media, and the area. The mean area of the umbilical cord was 35.73 ±23.04 mm2 (Mean ± SD) and the mean area of the Wharton’s jelly was 29.74 ± 19.26 mm2. There was a significant difference in the external diameter and wall thickness of the umbilical artery. Analyses showed that there was a significantly (P < 0.01) increased external diameter and wall thickness of umbilical artery in normal cases, compared to single umbilical artery cases. CONCLUSIONS There was a significant positive correlation between the gestational age and the external diameter of the umbilical cord. There was a significant difference in the external diameter of the umbilical cord between SUA cases (4.45 mm) and the other foetuses with normal umbilical cord (6.53 mm). There was a significantly increased external diameter, lumen diameter, wall thickness and area of umbilical vein in normal cases, compared to single umbilical artery cases. There was a significantly increased area of umbilical cord and area of Wharton’s jelly in normal umbilical cord foetuses than foetuses with a single umbilical artery. KEY WORDS Foetus, Umbilical Cord, Wharton’s Jelly, Umbilical Artery, Umbilical Vein, Light Microscopy

scholarly journals A Xeno-Free Strategy for Derivation of Human Umbilical Vein Endothelial Cells and Wharton’s Jelly Derived Mesenchymal Stromal Cells: A Feasibility Study toward Personal Cell and Vascular Based Therapy

2020 ◽  
Vol 2020 ◽  
pp. 1-8
Author(s):  
Hataiwan Kunkanjanawan ◽  
Tanut Kunkanjanawan ◽  
Veerapol Khemarangsan ◽  
Rungrueang Yodsheewan ◽  
Kasem Theerakittayakorn ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Umbilical Cord ◽  
Mesenchymal Stromal Cells ◽  
Stromal Cells ◽  
Umbilical Vein ◽  
Wharton’S Jelly ◽  
Human Umbilical Vein ◽  
Wharton's Jelly ◽  
Umbilical Vein Endothelial Cells

Coimplantation of endothelial cells (ECs) and mesenchymal stromal cells (MSCs) into the transplantation site could be a feasible option to achieve a sufficient level of graft-host vascularization. To find a suitable source of tissue that provides a large number of high-quality ECs and MSCs suited for future clinical application, we developed a simplified xeno-free strategy for isolation of human umbilical vein endothelial cells (HUVECs) and Wharton’s jelly-derived mesenchymal stromal cells (WJ-MSCs) from the same umbilical cord. We also assessed whether the coculture of HUVECs and WJ-MSCs derived from the same umbilical cord (autogenic cell source) or from different umbilical cords (allogenic cell sources) had an impact on in vitro angiogenic capacity. We found that HUVECs grown in 5 ng/ml epidermal growth factor (EGF) supplemented xeno-free condition showed higher proliferation potential compared to other conditions. HUVECs and WJ-MSCs obtained from this technic show an endothelial lineage (CD31 and von Willebrand factor) and MSC (CD73, CD90, and CD105) immunophenotype characteristic with high purity, respectively. It was also found that only the coculture of HUVEC/WJ-MSC, but not HUVEC or WJ-MSC mono-culture, provides a positive effect on vessel-like structure (VLS) formation, in vitro. Further investigations are needed to clarify the pros and cons of using autogenic or allogenic source of EC/MSC in tissue engineering applications. To the best of our knowledge, this study offers a simple, but reliable, xeno-free strategy to establish ECs and MSCs from the same umbilical cord, a new opportunity to facilitate the development of personal cell-based therapy.

scholarly journals A Gross and Histomorphological Study of the Umbilical Cord in Gestational Diabetes Mellitus

1970 ◽  
Vol 9 (1) ◽  
pp. 21-25 ◽  
Author(s):  
Sanjoy Kumar Chakraborty ◽  
BM Ali Yousuf ◽  
Laila Anjuman Banu ◽  
Khondker Manzare Shamim
Keyword(s):  
Diabetes Mellitus ◽  
Gestational Diabetes ◽  
Gestational Diabetes Mellitus ◽  
Umbilical Cord ◽  
Wharton’S Jelly ◽  
Control Group ◽  
Medical College ◽  
Wharton's Jelly ◽  
Mean Diameter ◽  
The Mean

Objective: Clinically the adverse effects of the diabetes on the out come of pregnancy are well established. The present study was designed to look for the impacts of gestational diabetes on the gross and histomorphological features of the umbilical cord. Type of study: A descriptive study having analytical components. Place and period of study: Department of Anatomy, Chittagong Medical College, Chittagong, from May 2006 to April 2007. Materials: Total fifty (50) umbilical cords with the placenta were collected within 36th to 40th weeks of gestation from Chittagong Medical College hospital and Memon maternity hospital of Chittagong City Corporation. Out of them 25 cords were from non diabetic pregnant mother (Control group) and 25 cords from mothers with gestational diabetes mellitus (GDM group). Method: After proper fixation with 10% formalin the diameter of the umbilical cord were measured with a vernier calipers in millimeters. Circumference, Cross-Sectional Area (CSA) of the umbilical cord and CSA of the Wharton’s jelly were measured by computerized micrometric method in millimeters and sq. millimeters respectively. Number of the umbilical vessels were examined and counted on the cut surface of the umbilical cord, later confirmed by histological examination. Presence or absence of the true knots were noted. Result: Among the gross morphological variables of the umbilical cord, the mean diameter was significantly larger in the GDM group than in the Control group (P< 0.05). Though the mean circumference, mean CSA of the umbilical cord and mean CSA of the Wharton's jelly were found to be higher in the GDM group than in the Control group, but the difference did not reach to the significant level. All the cords of the GDM group, the umbilical cord contained two arteries and one vein, whereas, two cords of the Control group, the umbilical cord had three arteries and one vein and one cord contain four arteries and one vein. No true knots were found in any groups. Pearson’s correlation test revealed significant positive correlation in both GDM and Control groups between mean diameter and circumference of the umbilical cord (r = 0.881, P = 0.000 & r = 0.689, P = 0.000) and between CSA of the umbilical cord and Wharton's jelly (r = 0.988, P = 0.000 & r = 0.990, P = 0.000). Key words: Umbilical cord; Gross and Histomorphology; GDM. DOI: http://dx.doi.org/ 10.3329/bja.v9i1.8143 Bangladesh Journal of Anatomy January 2011, Vol. 9 No. 1 pp 21-25

scholarly journals Immunomodulatory Properties of Wharton’s Jelly-Derived Mesenchymal Stem Cells from Three Anatomical Segments of Umbilical Cord

2021 ◽  
Vol 50 (6) ◽  
pp. 1715-1726
Author(s):  
Jezamine Lim ◽  
Sue Ping Eng ◽  
Wei Yen Yeoh ◽  
Yik Wan Low ◽  
Nur Mohd Shafwan bin Jusoh ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Umbilical Cord ◽  
Lymphocyte Proliferation ◽  
Mononuclear Cells ◽  
Wharton’S Jelly ◽  
Wharton's Jelly ◽  
Cell Based Therapy ◽  
Hla Dr ◽  
Significant Difference

Mesenchymal stem cells (MSCs) are multipotent progenitor cells that are reported to be immune-privileged and immune-evasive. MSCs are capable of differentiating into specific cell types for subsequent use in cell-based therapy. They express low levels of human leucocyte antigen (HLA)-ABC and no HLA-DR. Wharton’s jelly-derived MSCs (WJ-MSCs) were also found to express human leukocyte antigen G (HLA-G), which renders them immunosuppressive. This study aimed to determine whether cultured WJ-MSCs retain their immune-privileged and immune-evasive properties after cell differentiation, and whether these properties differ among MSCs derived from different anatomical segments of the umbilical cord. Umbilical cords of healthy pregnant mothers undergoing caesarean section were obtained and grouped by three anatomical segments: fetal, middle, and maternal segments. WJ-MSCs were isolated, culture-expanded, and differentiated into osteogenic cells. Expression of HLA-DR, HLA-ABC, and HLA-G were quantified using flow cytometry. Both undifferentiated and osteodifferentiated WJ-MSCs were subsequently co-cultured with allogeneic peripheral blood mononuclear cells with/without lipopolysaccharide (LPS) stimulation for five days. Lymphocyte proliferation assay was performed using carboxyfluorescein succinimidyl ester (CFSE) as a tracker. Our results showed no significant difference existed in the HLA profiles among WJ-MSCs from different segments and between WJ-MSCs with and without osteogenic differentiation. Mean levels for HLA-G, HLABC, and HLA-DR were 24.82±17.64, 52.50±18.41, and 1.00±1.68%, respectively. Stimulation with LPS and WJ-MSCs increased peripheral blooc mononuclear cells (PBMC) proliferation. However, PBMC proliferation was significantly lower when PBMCs were co-cultured with osteodifferentiated WJ-MSCs (p < .05; with LPS stimulation and p < .001 without LPS stimulation) than when they were co-cultured with undifferentiated WJ-MSCs. These findings suggest that cultured WJ-MSCs stimulate lymphocyte proliferation and are not immune-privileged. Osteodifferentiated WJ-MSCs reduced the immunogenicity of WJ-MSCs, and this reduction in PBMC proliferation was even more pronounced in the presence of LPS (p < .05). In conclusion, cultured WJ-MSCs are not immune-privileged. Osteodifferentiated WJ-MSCs are less immunogenic than undifferentiated WJ-MSCs, in which case hypoimmunogenicity is more profound under LPS-stimulated conditions.

scholarly journals Antenatal sonographic assessment of cross sectional area of umbilical cord components and its reference value in normal pregnancy

2018 ◽  
Vol 7 (10) ◽  
pp. 3924
Author(s):  
Khizer Hussain Afroze ◽  
Lakshmiprabha Subash ◽  
Anand S. H.
Keyword(s):  
Pregnant Women ◽  
Umbilical Cord ◽  
Reference Values ◽  
Gestational Age ◽  
Umbilical Vein ◽  
Cross Sectional Area ◽  
Wharton’S Jelly ◽  
Sectional Area ◽  
Cross Sectional ◽  
Wharton's Jelly

Background: Measuring the cross-sectional area of umbilical components in normal pregnant women helps in assessing the fetal abnormalities. Very few literatures were available on evaluation of reference values of cross sectional areas of umbilical cord components. The present study was conducted with the aim to determine the normal reference values of cross sectional areas of umbilical arteries, umbilical vein and Wharton’s jelly and to correlate them with the gestational age of the fetus.Methods: A cross sectional study was conducted on 300 normal pregnant women at the Department of Radiodiagnosis, Sri Siddhartha Medical College, Tumakuru, Karnataka to assess the reference range of cross sectional areas of umbilical cord arteries, umbilical vein and Wharton’s jelly at different gestational age of the fetus to analyze their growth.Results: A statistically significant correlation was observed between cross sectional areas of umbilical artery and vein and gestational age before and after 34 weeks (p=0.005 and 0.006 respectively) but no significant correlation was noticed with the cross-sectional area of Wharton’s jelly (p=0.088).Conclusions: Cross sectional area measurements of umbilical cord components can be considered as important tools for estimation of fetal growth.

scholarly journals Efficient Non-Viral Gene Modification of Mesenchymal Stromal Cells from Umbilical Cord Wharton’s Jelly with Polyethylenimine

Pharmaceutics ◽  
2020 ◽  
Vol 12 (9) ◽  
pp. 896
Author(s):  
Ana Isabel Ramos-Murillo ◽  
Elizabeth Rodríguez ◽  
Karl Beltrán ◽  
Cristian Ricaurte ◽  
Bernardo Camacho ◽  
...  
Keyword(s):  
Umbilical Cord ◽  
Mesenchymal Stromal Cells ◽  
Stromal Cells ◽  
Transfection Efficiency ◽  
Adipogenic Differentiation ◽  
Wharton’S Jelly ◽  
Viral Gene ◽  
Human Umbilical Cord ◽  
Wharton's Jelly ◽  
Significant Difference

Mesenchymal stromal cells (MSC) derived from human umbilical cord Wharton’s jelly (WJ) have a wide therapeutic potential in cell therapy and tissue engineering because of their multipotential capacity, which can be reinforced through gene therapy in order to modulate specific responses. However, reported methodologies to transfect WJ-MSC using cationic polymers are scarce. Here, WJ-MSC were transfected using 25 kDa branched- polyethylenimine (PEI) and a DNA plasmid encoding GFP. PEI/plasmid complexes were characterized to establish the best transfection efficiencies with lowest toxicity. Expression of MSC-related cell surface markers was evaluated. Likewise, immunomodulatory activity and multipotential capacity of transfected WJ-MSC were assessed by CD2/CD3/CD28-activated peripheral blood mononuclear cells (PBMC) cocultures and osteogenic and adipogenic differentiation assays, respectively. An association between cell number, PEI and DNA content, and transfection efficiency was observed. The highest transfection efficiency (15.3 ± 8.6%) at the lowest toxicity was achieved using 2 ng/μL DNA and 3.6 ng/μL PEI with 45,000 WJ-MSC in a 24-well plate format (200 μL). Under these conditions, there was no significant difference between the expression of MSC-identity markers, inhibitory effect on CD3+ T lymphocytes proliferation and osteogenic/adipogenic differentiation ability of transfected WJ-MSC, as compared with non-transfected cells. These results suggest that the functional properties of WJ-MSC were not altered after optimized transfection with PEI.

A case of a four-vessel umbilical cord: don’t stop counting at three!

2012 ◽  
Vol 1 (1-2) ◽  
Author(s):  
Geneviève Donata Koolhaas ◽  
Martine Helene Hollander ◽  
Harry Molendijk
Keyword(s):  
Umbilical Cord ◽  
Umbilical Artery ◽  
Congenital Abnormalities ◽  
Umbilical Vein ◽  
Daily Practice ◽  
Congenital Defects ◽  
Ultrasound Screening ◽  
Single Umbilical Artery ◽  
Antenatal Ultrasound ◽  
Substantial Morbidity

AbstractThere are various numerical abnormalities of umbilical cord vessels known in the literature, the single umbilical artery being the most prevalent. A four-vessel umbilical cord is found less frequently, and moreover, is less well-known in daily practice. A persistent right umbilical vein, however, can be associated with potentially serious congenital defects. A case of a four-vessel umbilical cord containing two arteries and two veins is presented. The literature on this subject reports both a differentiation between the two variants, intrahepatic and extrahepatic, which can be distinguished during antenatal ultrasound screening, and a possible association with congenital abnormalities, some of which can carry substantial morbidity and mortality. Although the incidence of a four-vessel umbilical cord is low, its presence should be considered both during routine antenatal ultrasound screening and on physical examination of any neonate.

scholarly journals Treatment of Knee Osteoarthritis with Intraarticular Umbilical Cord-Derived Wharton’s Jelly: A Case Report

2021 ◽  
Vol 14 (9) ◽  
pp. 883
Author(s):  
Ashim Gupta ◽  
Hugo C. Rodriguez ◽  
Anish G. Potty ◽  
Howard J. Levy ◽  
Saadiq F. El-Amin III
Keyword(s):  
Knee Osteoarthritis ◽  
Umbilical Cord ◽  
Likert Scale ◽  
Wharton’S Jelly ◽  
Post Injection ◽  
X Rays ◽  
Wharton's Jelly ◽  
Sf 36 ◽  
Significant Difference

We present the case of a 27-year-old male with grade II knee osteoarthritis (OA) that was intraarticularly injected with a 2 mL umbilical cord-derived Wharton’s jelly (UC-derived WJ) formulation. The patients’ baseline radiographs were taken and baseline numeric pain rating scale (NPRS), knee injury and osteoarthritis outcome score (KOOS), 7-point Likert scale, and a 36-item short form survey (SF-36) were recorded. The NPRS was re-recorded immediately after the injection, and at 24 h, 48 h, 1 week, 6 weeks, and at 3 months follow-up post-injection. The KOOS and 7-point Likert scale was re-recorded at the patients’ 1week, 6 week, and 3month follow-up, and SF-36 was re-recorded at 3 months. A final set of X-rays were also performed at 3 months follow-up post-injection. No adverse effects from the injection were reported over the duration of the study. No significant difference nor progression in OA on X-rays compared to baseline was observed. NPRS decreased by 50% and the 7-point Likert scale increased to Extremely Satisfied. KOOS increased overall by 10% and the SF-36 overall change was 25%. These results indicate the potential application of UC-derived WJ in the treatment of knee OA. Larger, long term, non-randomized and randomized control trials are warranted to adequately assess the safety and efficacy of UC-derived WJ and ultimate clinical use.

scholarly journals Partially four-vessel umbilical cord: about a diagnosed case at Panzi hospital

2019 ◽  
Vol 9 (1) ◽  
pp. 450
Author(s):  
Boengandi Walala D. ◽  
Nyakio Ngeleza O. ◽  
Mukanire Ntakwinja B. ◽  
Katenga Bosunga G. ◽  
Mukwege Mukengere D.
Keyword(s):  
Umbilical Cord ◽  
Congenital Anomalies ◽  
Umbilical Artery ◽  
Umbilical Vein ◽  
Maternity Ward ◽  
Dr Congo ◽  
Single Umbilical Artery ◽  
South Kivu ◽  
The Town

Umbilical cord malformations are described in the literature; and it's about the umbilical artery and the umbilical vein. There are sometimes cases of a single umbilical artery. In contrast, the number of vessels in the umbilical cord can increase by three to four. A supernumerary umbilical vein is particularly rare and is usually found in association with congenital anomalies; sometimes the supernumerary umbilical vein is associated with no malformation. We report a case of umbilical cord having a four-vessel part diagnosed at the maternity ward of Panzi Hospital, in the town of Bukavu, South Kivu, DR Congo.

Prenatal observation of fragmented duplication of umbilical vein: isolated and associated with single umbilical artery

2017 ◽  
Author(s):  
I.I. Ryabov , K.F. Yusupov , E.À. Pryashnikova
Keyword(s):  
Umbilical Cord ◽  
Umbilical Artery ◽  
Umbilical Vein ◽  
Prenatal Ultrasound ◽  
Ultrasound Diagnosis ◽  
Single Umbilical Artery ◽  
First Case

Two cases of prenatal ultrasound diagnosis of fragmented duplication of umbilical vein are presented. In the first case the fragmented duplication of umbilical vein in the central part of umbilical cord coupled with a single umbilical artery. Thus, if the examination of a number of vessels is carried out on the circumscribed part of umbilical cord only, for example at fragmented duplication of umbilical vein, the single umbilical artery may not be diagnosed.

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