scholarly journals Wound healing mechanisms in rats with streptozotocin-induced diabetes mellitus

2018 ◽  
Vol 64 (5) ◽  
pp. 292-298
Author(s):  
Evgeniy V. Ivanov ◽  
Svetlana A. Gavrilova ◽  
Maria P. Morozova ◽  
Ekaterina M. Klochihina ◽  
Aleksey K. Erdyakov ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Wound Healing ◽  
Tissue Repair ◽  
Control Group ◽  
Withdrawal Time ◽  
Diabetes Group ◽  
Citrate Buffer ◽  
Ki 67 ◽  
Streptozotocin Induced Diabetes ◽  
Β2 Adrenoreceptors

Background. Wound healing disorders and formation of diabetic foot, a severe disabling complication of diabetes mellitus, are accompanied by nervous system impairment and/or ischemia. Objective — the study was aimed at assessing the effect of peripheral innervation disorders on the regulation of tissue repair in the streptozotocin-induced rat model of diabetes mellitus. Material and methods. The study was carried out in male white outbred rats (n=70). The animals were wounded 42 days after induction of diabetes by injecting streptozotocin (diabetes group; this group received insulin Levemir at a dose of 2 units/kg in saline subcutaneously to reduce mortality), or after injection of citrate buffer (CB group). Skin samples were taken on day 8, 16, and 24 after wound modeling. Pain sensitivity was assessed in all animals. The resulting skin fragments were fixed, dehydrated, and embedded in paraffin according to standard procedures. Sections were stained with hematoxylin and eosin, antibodies specific for Ki-67, α1, β1, and β2-adrenoreceptors were used for immunohistochemical staining. Intact animals were used as an additional control group. Results. Tail withdrawal time measured on day 56 was higher in DM group rats as compared to the control group (p=0.017). CB group demonstrated a tendency towards more rapid wounds healing than diabetic animals, although the difference was not statistically significant due to wide scatter of data in the DM group (p=0.64). The intensity of staining for Ki67 was lower in the DM group (p=0.045). Reduced density of β2-adrenoreceptors was observed at the areas remote from the wound in CB group rats. Conclusion The results show no correlation between altered innervation and impaired tissue repair in rats with streptozotocin-induced diabetes.

scholarly journals Morphology of bronchial epithelium in rodent streptozotocin-induced diabetes mellitus

2013 ◽  
Vol 16 (4) ◽  
pp. 44-48
Author(s):  
Oksana Anatolyevna Pivovarova ◽  
Boris Nikitich Mankovsky
Keyword(s):  
Diabetes Mellitus ◽  
Body Weight ◽  
Wistar Rats ◽  
Bronchial Epithelium ◽  
Secretory Cells ◽  
Control Group ◽  
Partial Loss ◽  
Citrate Buffer ◽  
Single Intraperitoneal Injection ◽  
Streptozotocin Induced Diabetes

Aim.  To study the morphology of bronchial epithelium in a rodent streptozotocin-induced (STZ) diabetes mellitus. Materials and Methods. . Diabetes mellitus was introduced in 47 white Wistar rats aged 5?6 months (body weight 234.0?2.64 g). 43 white Wistar rats of the same age were used as control subjects (body weight 242.0?2.13). Diabetes was induced by single intraperitoneal injection of STZ (SIGMA, USA) 60 mg/kg in 0.1 M citrate buffer, pH 4.5. Results. A statistically significant decrease in the total epithelial area by 25.9% was observed in the study group, accompanied by a reduction of the supranuclear zone by 22.1% vs. the control group. Conclusion. We found that bronchial mucous membrane in rodents with STZ-induced diabetes mellitus exhibits signs of atrophy and partial loss of mucous production by bronchial secretory cells.

scholarly journals Keratinocytes differentiation and wound healing in rats with streptozotocin – induced diabetes and severe hyperglycemia

2020 ◽  
Vol 23 (1) ◽  
pp. 19-28 ◽  
Author(s):  
Evgeniy V. Ivanov ◽  
Maria P. Morozova ◽  
Ekaterina M. Rzhavina ◽  
Anna M. Gorbacheva ◽  
Svetlana A. Gavrilova ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Wound Healing ◽  
Its Region ◽  
Repair Process ◽  
Diabetic Rat ◽  
Control Group ◽  
Intact Skin ◽  
Skin Repair ◽  
Diabetes Model ◽  
Streptozotocin Induced Diabetes

BACKGROUND: Diabetes mellitus leads to disruption of the skin repair processes, but the leading mechanisms of this pathology have not yet been identified. In this regard, in our work, we decided to check how hyperglycaemia affects the process of keratinocyte phenotype changes during wound healing. AIMS: To study the effect of hyperglycaemia on wound healing and differentiation of keratinocytes in a rat streptozotocin-induced diabetes model. MATERIALS AND METHODS: Diabetes mellitus was induced in rats by using streptozotocin, 65 mg / kg, intraperitoneally, once. The wound was applied in the supra-scapular region on the 42nd day, after which (after 8, 16, and 24 days) the repair process was evaluated using histological methods. Immunohistochemistry was used to evaluate the expression of cytokeratin-10 and cytokeratin-17. RESULTS: In rats with diabetes mellitus, wound healing slowed down in the later stages, compared with the control group. In general, wound healing was accompanied by an increase in the expression of cytokeratin-10 in its region compared with intact skin, and contractile keratinocytes activation was disrupted in diabetic rat wounds. CONCLUSIONS: Hyperglycaemia slightly slows wound healing in rats and impairs contractile keratinocytes activation.

scholarly journals EFFECTS OF PROBIOTICS SUPPLEMENTATION ON SKIN WOUND HEALING IN DIABETIC RATS

2020 ◽  
Vol 33 (1) ◽  
Author(s):  
Letícia Fuganti CAMPOS ◽  
Eliane TAGLIARI ◽  
Thais Andrade Costa CASAGRANDE ◽  
Lúcia de NORONHA ◽  
Antônio Carlos L. CAMPOS ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Wound Healing ◽  
Type I Collagen ◽  
Chronic Wounds ◽  
Skin Wound ◽  
Diabetic Rats ◽  
Control Group ◽  
Type I ◽  
Collagen Deposition ◽  
Skin Wound Healing

ABSTRACT Background: Chronic wounds in patients with Diabetes Mellitus often become incurable due to prolonged and excessive production of inflammatory cytokines. The use of probiotics modifies the intestinal microbiota and modulates inflammatory reactions. Aim: To evaluate the influence of perioperative supplementation with probiotics in the cutaneous healing process in diabetic rats. Methods: Forty-six rats were divided into four groups (C3, P3, C10, P10) according to the treatment (P=probiotic or C=control, both orally administered) and day of euthanasia, 3rd or 10th postoperative days. All rats were induced to Diabetes Mellitus 72 h before starting the experiment with alloxan. Supplementation was initiated five days before the incision and maintained until euthanasia. Scalpel incision was guided by a 2x2 cm mold and the wounds were left to heal per second-intention. The wounds were digitally measured. Collagen densitometry was done with Picrosirius Red staining. Histological parameters were analyzed by staining by H&E. Results: The contraction of the wound was faster in the P10 group which resulted in a smaller scar area (p=0.011). There was an increase in type I collagen deposition from the 3rd to the 10th postoperative day in the probiotic groups (p=0.016), which did not occur in the control group (p=0.487). The histological analysis showed a better degree of healing in the P10 group (p=0.005), with fewer polymorphonuclear (p<0.001) and more neovessels (p=0.001). Conclusions: Perioperative supplementation of probiotics stimulates skin wound healing in diabetic rats, possibly due to attenuation of the inflammatory response and increased neovascularization and type I collagen deposition.

Evaluation of cardiac function in fetuses of mothers with gestational diabetes

2019 ◽  
Vol 29 (10) ◽  
pp. 1264-1267 ◽  
Author(s):  
Muhammad Mohsin ◽  
Saleem Sadqani ◽  
Kamran Younus ◽  
Zahra Hoodbhoy ◽  
Salima Ashiqali ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Gestational Diabetes ◽  
Cardiac Function ◽  
Gestational Diabetes Mellitus ◽  
Diastolic Function ◽  
Left Atrial ◽  
Myocardial Hypertrophy ◽  
Control Group ◽  
Diabetes Group ◽  
Diabetic Mothers

AbstractObjective:The purpose of this study was to assess fetal cardiac function in normal fetuses (control group) compared to those who are exposed to gestational diabetes mellitus using different echocardiographic measurements, and to explore the application of left atrial shortening fraction in determination of fetal diastolic function with gestational diabetes mellitus.Methods:A total of 50 women with gestational diabetes and 50 women with a healthy pregnancy were included in the study. Fetal echocardiography was performed and structural as well as functional fetal cardiac parameters were measured. Data were compared between with or without fetal myocardial hypertrophy and the control group.Results:In the study group, out of 50 fetuses of gestational diabetic mothers, 18 had myocardial hypertrophy and 32 had normal septal thickness. Gestational age at time of examination did not differ significantly between the control and gestational diabetes group (p = 0.55). Mitral E/A ratio was lower in gestational diabetes group as compared to the control (p < 0.001). Isovolumetric relaxation and contraction times and myocardial performance index were greater in fetuses of gestational diabetic mothers (p < 0.001). In fetuses of gestational diabetic mothers with myocardial hypertrophy, left atrial shortening fraction was lower as compared to those without myocardial hypertrophy and those of the control group (p < 0.001).Conclusions:The results of this study suggest that fetuses of gestational diabetic mothers have altered cardiac function even in the absence of septal hypertrophy, and that left atrial shortening fraction can be used as a reliable alternate parameter in the assessment of fetal diastolic function.

Physiology of Wound Healing and Risk Factors that Impede the Healing Process

1990 ◽  
Vol 1 (3) ◽  
pp. 545-552 ◽  
Author(s):  
Susan O’Brien Norris ◽  
Barbara Provo ◽  
Nancy A. Stotts
Keyword(s):  
Diabetes Mellitus ◽  
Wound Healing ◽  
Tissue Repair ◽  
Wound Repair ◽  
Healing Process ◽  
Critically Ill Patient ◽  
Wound Complications ◽  
Care Environment ◽  
Patient Morbidity ◽  
Illness Knowledge

In the critically ill patient, wound repair can be impeded by processes inherent to the illness, its treatment, and the critical care environment. This vulnerability to wound complications increases patient morbidity and mortality as well as length of stay, resource consumption, and hospital cost. The physiology of wound healing and factors that impede wound repair are discussed. Those factors commonly seen in critical illness include advanced age, diabetes mellitus, compromised immunocompetence, inadequate perfusion, and oxygenation, infection, malnutrition, obesity, and preoperative illness. Knowledge of management of the physiologic factors that affect wound healing enables the nurse to maximize tissue repair and prevent wound complications

scholarly journals Effect of Abelmoschus esculentus (okra)-based diet on streptozotocin-induced diabetes mellitus in adult Wistar rats

2020 ◽  
Vol 19 (8) ◽  
pp. 1737-1743
Author(s):  
Patrick O. Uadia ◽  
Isaac O. Imagbovomwan ◽  
Kelly Oriakhi ◽  
Ikechi G. Eze
Keyword(s):  
Diabetes Mellitus ◽  
Normal Control ◽  
Wistar Rats ◽  
Diabetic Control ◽  
Normal Diet ◽  
Abelmoschus Esculentus ◽  
Control Group ◽  
Β Cells ◽  
Streptozotocin Induced Diabetes ◽  
Ad Libitum

Purpose: To evaluate the effects of an okra-based diet on streptozotocin-induced diabetes mellitus in adult Wistar rats and its mechanism of action.Methods: Wistar rats (6) were administered streptozotocin (50 mg/kg ip) after an overnight fast. Upon confirmation of diabetes mellitus, the animals were fed ad libitum for 21 days with formulated okrabased test diet in place of normal diet. The rats treated similarly with streptozotocin and fed ad libitum with the normal diet served as diabetic control while rats fed on normal diet and not treated with streptozotocin served as the negative control. Thereafter, the rats were sacrificed, fasting bloodcollected and analysed for glucose concentration and biochemical parameters. Pancreas was also excised for histopathological studies.Results: There was a significant increase in body weight, HDL-cholesterol (p< 0.05) but significant decrease in blood glucose (p < 0.05), serum total cholesterol, triglyceride, LDL-cholesterol and VLDLcholesterol concentrations in the okra-fed diabetic treated rats when compared to the diabetic control group. Furthermore, superoxide dismutase activity (SOD) was significantly higher in the diabetic control, and reduced significantly when fed with okra-based diet (p < 0.05). Catalase (CAT) activity was significantly (p < 0.05) decreased in diabetic control and treated group, whereas it was significantly (p < 0.05) increased in normal control rats. There was a significant (p < 0.05) decrease in reduced glutathione levels. The significant (p< 0.05) increase in malondialdehyde in the diabetic group was significantly decrease in the diabetic rats fed with okra-diet. Also serum insulin level was significantly (p < 0.05) increased and serum α amylase activity was significantly (p < 0.05) decrease in the diabetic treated rats. Histology results show that there was damage to the β cells of the pancreas in the diabetic control when compared to normal control, but rats fed okra diet was able to regenerate endocrine β cells.Conclusion: Okra-based diet lowers hyperglycaemia as well as regenerate/repair endocrine β-cells and exocrine tissues of the pancreas damage by streptozotocin Keywords: Abelmoschus esculentus, Diabetes mellitus, anti-diabetic, Insulin, α-amylase, Mechanism

scholarly journals EFFECT OF THE ENZYME-CONTAINING POLYMERIC NANOPARTICLES ON MMP-2 ACTIVITY DURING BURN WOUND HEALING IN RATS WITH STREPTOZOTOCIN-INDUCED DIABETES

2021 ◽  
Vol 17 (2) ◽  
pp. 12-19
Author(s):  
O.I. Myronenko ◽  
T.I. Panova ◽  
L.V. Natrus ◽  
S.V. Verevka
Keyword(s):  
Diabetes Mellitus ◽  
Extracellular Matrix ◽  
Wound Healing ◽  
Gelatin Zymography ◽  
Matrix Metalloproteinase 2 ◽  
Diabetic Wound ◽  
Burn Wound ◽  
Impaired Wound Healing ◽  
Chronic Hyperglycemia ◽  
Streptozotocin Induced Diabetes

Relevance. Diabetic foot syndrome is a common complication that is characterized by the development of chronic ulcers. Among the mechanisms of impaired wound healing, the leading role is played by disturbance of extracellular matrix homeostasis: chronic hyperglycemia, on the one hand, promotes the formation of so-called advanced glycation end products (AGEs), which mediate pro-inflammatory activation of immune cells, and on the other hand, inhibits fibroblasts proliferation and collagen production, disrupts the migration of keratinocytes and endothelial cells. Therefore, the elimination of AGEs is a pathogenetic approach in diabetic wound treatment. For this purpose, a composite consisting of polyspecific microbial proteinases fixed on polymeric porous nanoparticles was developed. The activity of matrix metalloproteinase-2 (MMP-2) was chosen as a prognostic indicator of chronic wound healing. Objective: to study the activity of MMP-2 in the tissues of the burn wound of rats with simulated diabetes mellitus under the influence of enzyme-containing nanoparticles. Materials and methods. N = 48 Wistar rats were used in the experiment. Diabetes mellitus was induced by administration of 50 mg/kg of streptozotocin. To model the wound in rats, a standard animal model of thermal burns by Walker and Mason was used. Thermal damage corresponded to the II-IIIA degree of burns, and occupied 19±1.6% of the total area of ​​animal skin. Rats were divided into two groups of 24 animals each: the DM group did not receive any treatment, and rats from the DM+T group were daily applied to the burn wound with the mentioned composite (enzyme-containing nanoparticles). Animals were removed from the experiment on days 3, 7, 14 and 21 of observation. The activity of MMP-2 in the tissues of the burn wound of diabetic rats was studied by gelatin zymography, expressed in arbitrary units (AU). Statistical data processing was performed in the software package SPSS Statistics Base, v.22 with Student and Scheffe tests. Results. The level of activity of MMP-2 in the tissues of the burn wound of rats in the DM group on the 3rd day of the study was 4.9 ± 1.3 AU, increased by 7 days (p <0.01) and reached a maximum level of 52.55 ± 3.06 AU at day 14 (p <0.01). On day 21, the activity of the test enzyme decreased by 8.5 AU (p <0.01), compared to day 14. On day 3 of the study in the DM+T group, the activity of MMP-2 in the diabetic wound was 15.93 ± 2.68 AU and gradually decreased (p <0.01) to 5.67 ± 2.67 AU on day 14. However, on day 21, the second peak (p <0.01) of the activity of the studied enzyme was observed - 33.64 ± 4.1 AU. When comparing the two groups (DmM and DM+T) on day 3 of the study, the activity of MMP-2 in the tissues of the burn wound of rats in the DM+T group was three times higher (p <0.01) than in the DM group. But from the 7th day the activity of MMP-2 in the DM group was higher than the DM+T group. On day 21 of the study, the level of MMP-2 in the DM group remained higher (p <0.01) than in the DM+T group. Conclusions. The use of enzyme-containing nanoparticles provides effective degradation of glycosylated components of the extracellular matrix (AGEs), thereby reducing the inflammatory process and activity of MMP-2, and promoting wound healing in rats with streptozotocin-induced diabetes.

scholarly journals Effect of long-term hyperglycemia on cornea and retina morphology in rats with streptozotocin-induced diabetes mellitus

2021 ◽  
pp. 42-47
Author(s):  
А.А. Панов ◽  
Е.М. Ржавина ◽  
М.П. Морозова ◽  
А.К. Ердяков ◽  
С.А. Гаврилова
Keyword(s):  
Diabetes Mellitus ◽  
Ketone Bodies ◽  
Morphological Changes ◽  
Posterior Part ◽  
Male Rats ◽  
Control Group ◽  
Blood Concentrations ◽  
Central Retina ◽  
Streptozotocin Induced Diabetes

Цель исследования - изучение динамики морфологических изменений роговицы и заднего отдела глаза крыс при длительной гипергликемии. Методика. Исследование выполнено на 36 самцах крыс Wistar. Сахарный диабет индуцировали внутрибрюшинной инъекцией стрептозотоцина (65 мг/кг), после чего ежедневно вводили подкожно малые дозы инсулина (2 ЕД/кг). На 50-е, 58-е и 66-е сут эксперимента производили энуклеацию глаз у глубоко наркотизированных животных. Гистологические срезы фрагментов глаз окрашивали гематоксилин-эозином, проводили морфометрию параметров роговицы и сетчатки. Результаты. Средняя концентрация глюкозы и кетоновых тел крови в группе сахарного диабета составила 29,8 ммоль/л и 0,889 ммоль/л, в контрольной группе - 6,2 ммоль/л и 0,847 ммоль/л соответственно. Анализ гистологических срезов глаз выявил признаки отека роговицы, хориоидеи и наружных слоев центральных отделов сетчатки до появления других качественных и количественных морфологических изменений. Заключение. Оценка толщины роговицы, хориоидеи и наружных слоев центральных отделов сетчатки может служить предиктором развития диабетической ретинопатии. The aim was to study morphological changes in the cornea and the posterior part of rat eye during prolonged hyperglycemia. Methods. The study was performed on 36 Wistar male rats. Diabetes mellitus was induced by an injection of streptozotocin (65 mg/kg, i.p.) followed by daily injections of low doses of insulin (2 U/kg, s.c.). Eyes were enucleated from deeply anesthetized rats on days 50, 58, and 66 of the experiment. Histologic sections were stained with hematoxylin-eosin, and morphometry of the cornea and the retina was performed. Results. Mean blood concentrations of glucose and ketone bodies were 29.8 mmol/L and 0.889 mmol/L, respectively, in the diabetic group and 6.2 mmol/L and 0.847 mmol/L, respectively, in the control group. The histological analysis revealed signs of edema in the cornea, choroid and outer layers of the central retina, which preceded other morphological changes. Conclusion. Evaluating thickness of the cornea, choroid and outer layers of the central retina may serve for prediction of diabetic retinopathy.

scholarly journals Evaluation of radioprotective effect of carnosine (beta- alanyl-1- histidine) on the wound healing in rats

2005 ◽  
Vol 13 (3) ◽  
pp. 253-258 ◽  
Author(s):  
Rosana Aramaki Tanaka ◽  
Flávia Maria de Moraes Ramos ◽  
Solange Maria de Almeida ◽  
Mário Roberto Vizioli ◽  
Frab Norberto Bóscolo
Keyword(s):  
Wound Healing ◽  
Surgical Procedure ◽  
Tissue Repair ◽  
Radioprotective Effect ◽  
Male Rats ◽  
Whole Body ◽  
Control Group ◽  
Experimental Conditions ◽  
Anterior Dorsal ◽  
Radioprotective Substance

The purpose of this study was to evaluate the radioprotective effect of carnosine (beta- alanyl-1-histidine) on the wound healing in rats. Therefore, 48 male rats were submitted to a surgical procedure to perform a rectangular wound in the anterior-dorsal region. The animals were divided into 4 experimental groups randomly chosen: control; irradiated; carnosine irradiated and carnosine group. The irradiated and carnosine irradiated group were exposed to a dose (6Gy) of gamma irradiation, in the whole body, 72 hours after surgery. The carnosine and carnosine irradiated groups, in addition to the surgical procedure and the irradiation, received two doses of carnosine aqueous solution, the first one being injected 48 hours after surgery, and the second one 1 hour and 30 minutes before irradiation. The tissue repair of the 4 groups was evaluated at 4, 7, 14, and 21 days after inflicting the wound, by morphological, histochemical and histophysical methods. At all examined periods, it could be observed that the animals from the carnosine irradiated group presented a better developed granulation tissue than the irradiated group and closely similar to that of the control group. Thus, under the experimental conditions used, it was possible to conclude that carnosine is an effective radioprotective substance.

scholarly journals Deteriorated gene expression of selected calcium transporters in streptozotocin-induced diabetic hearts of Wistar rats

2018 ◽  
Vol 65 (2) ◽  
pp. 1-3
Author(s):  
M. Dragún ◽  
G. Dóka ◽  
M. Máťuš ◽  
P. Křenek ◽  
J. Klimas
Keyword(s):  
Diabetes Mellitus ◽  
Calcium Homeostasis ◽  
Wistar Rats ◽  
Control Group ◽  
Mrna Levels ◽  
Short Term ◽  
Gene Expressions ◽  
Citrate Buffer ◽  
Total N

Abstract Aim: The aim is to identify the possible changes in the expression of genes, that regulate calcium homeostasis in cardiomyocytes in diabetes mellitus. Methods: Male Wistar rats were randomized into two experimental protocols: short-term 5-days streptozotocin-induced diabetes protocol with 20 weeks old animals at the end of the protocol (total N = 20) and long-term 4-weeks protocol with 18 weeks of age at the end of the protocol (total N = 38). 50 mg/kg of streptozotocin (STZ) was administered in both protocols by a single intraperitoneal injection in 0,1M citrate buffer (pH = 4.5). Control group (CON) received only vehiculum. Gene expressions in samples of left heart ventricle were measured by RT-qPCR method. Results: The expression of SERCA2a in short-term protocol was decreased. In long-term protocol, decreased SERCA2a, TRPC4 and TRPC6 mRNA levels were observed (*p < 0.05). SERCA2a and TRPC4 mRNA levels exhibited statistical monotonic correlation in STZ-treated group in long-term protocol. Conclusions: In diabetes mellitus, the calcium homeostasis in cardiomyocytes is altered and there could be a relation between alteration of internal sarcoplasmatic stores and store-operated calcium entry.

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