scholarly journals Activity of hydrolytic enzymes of innate immunity in bacterial and coronaviral pneumonia.

2021 ◽  
pp. 6-12
Author(s):  
V. Zemko ◽  
A. Frolova ◽  
Y. Zemko ◽  
V. Okulich ◽  
T. Lepteeva
Keyword(s):  
Innate Immunity ◽  
Blood Serum ◽  
Healthy Volunteers ◽  
Bacterial Pneumonia ◽  
Comparison Group ◽  
Hydrolytic Enzymes ◽  
Serum Enzymes ◽  
Enzymatic Activities ◽  
Group A ◽  
Deoxyribonuclease Activity

The aim of this work was to study the enzymatic activities of hydrolases in the blood serum of patients with bacterial pneumonia, pneumonia caused by SARS-CoV-2 and practically healthy volunteers who made up the comparison group. A number of blood serum enzymes related to innate immunity were determined: lysozyme, elastase, BAPNAamidase and deoxyribonuclease activity. Results: it was shown that blood serum in pneumonia caused by SARS-CoV-2 has the highest level of elastase and lysozyme activities, which significantly (p<0,001) exceeded the corresponding activities of sera amongst patients with bacterial pneumonia and in the comparison group. It was also found that trypsin-like activity of blood serum was increased in bacterial pneumonia in comparison with pneumonia caused by SARS-CoV-2 (p <0,001). There were no statistically significant differences in the level of deoxyribonuclease activity of blood serum among analyzed groups.

Selflessness in Anorexia Nervosa as Reflected in the Rorschach Comprehensive System

2012 ◽  
Vol 33 (1) ◽  
pp. 78-93 ◽  
Author(s):  
Gili Curiel-Levy ◽  
Laura Canetti ◽  
Esti Galili-Weisstub ◽  
Myrna Milun ◽  
Eitan Gur ◽  
...  
Keyword(s):  
Multivariate Analysis ◽  
Anorexia Nervosa ◽  
Analysis Of Variance ◽  
Clinical Observation ◽  
Comparison Group ◽  
Comprehensive System ◽  
Multivariate Analysis Of Variance ◽  
Rorschach Comprehensive System ◽  
Group A

This study examines the expression of selflessness – the tendency to ignore one’s own needs and serve others’ needs – in Rorschach protocols of women suffering from anorexia nervosa. The protocols of 35 women suffering from anorexia nervosa were compared to 30 protocols of a psychiatric comparison group. A multivariate analysis of variance over five variables (AG, PER, PHR, COP, and GHR) was significant: Anorexic patients showed higher characteristics of selflessness compared to the psychiatric comparison group. These findings contribute to the validation of the Rorschach technique and to the clinical observation of selflessness in anorexic patients, and they emphasize specific characteristics in the treatment of anorexia nervosa patients.

Abstract 17638: Systemic Cardiovascular Effects of Intravenous Urocortin 2 and Urocortin 3 in Patients with Heart Failure and Healthy Volunteers

Circulation ◽  
2014 ◽  
Vol 130 (suppl_2) ◽  
Author(s):  
Colin G Stirrat ◽  
Sowmya Venkatasubramanian ◽  
Tania Pawade ◽  
Andrew Mitchell ◽  
Anoop Shah ◽  
...  
Keyword(s):  
Heart Failure ◽  
Blood Pressure ◽  
Cardiac Index ◽  
Healthy Volunteers ◽  
Diastolic Blood Pressure ◽  
Cardiovascular Effects ◽  
Peripheral Vascular ◽  
Patients With Heart Failure ◽  
Group A ◽  
Urocortin 3

Introduction: Urocortin 2 (UCN 2) and urocortin 3 (UCN 3) are endogenous peptide hormones with an emerging role in the pathophysiology and treatment of heart failure. For the first time, we examined the systemic cardiovascular effects of both UCN 2 and UCN 3 in healthy volunteers and patients with heart failure. Methods: Seven healthy volunteers (Group A) and nine patients with stable chronic heart failure (Group B, New York Heart Association class II and III, left ventricular ejection fraction <35%) on optimal medical therapy underwent non-invasive oscillometric sphygmomanometry and impedance cardiography during incremental intravenous infusions of sodium nitroprusside (0.15/0.5/1.5 μg/kg/min), UCN 2 (0.16/0.48/1.6 μg/min), UCN 3 (5/15/50 μg/min) and saline placebo in a randomised double blind two-way cross over study. Results: Other than diastolic blood pressure (78 vs 72 mmHg for Group A and B respectively, p<0.05), haemodynamic variables were similar at baseline of each infusion and were unchanged by saline placebo infusion (p>0.05 for all). SNP, UCN2 and UCN 3 infusions increased heart rate and cardiac index, and reduced systolic and diastolic blood pressure and peripheral vascular resistance index (PVRI) in both healthy volunteers and patients with heart failure (p<0.05 for all; see Figure 1). There were no significant differences in the changes in cardiac index or PVRI between healthy volunteers and patients with heart failure during either UCN 2 or UCN 3 infusions (p>0.05). Conclusion: Intravenous UCN 2 and especially UCN 3 increase cardiac output and reduce peripheral vascular resistance. This favourable haemodynamic profile suggests that UCN 2 and UCN 3 hold exciting therapeutic potential for the treatment of acute heart failure.

scholarly journals Surgical treatment of peripheral hepatic cholangiocarcinoma with a visceral vein invasion

2020 ◽  
Vol 87 (7-8) ◽  
pp. 3-9
Author(s):  
О. Yu. Usenko ◽  
О. V. Hrynenko ◽  
А. І. Zhylenko ◽  
О. О. Popov ◽  
А. V. Husiev ◽  
...  
Keyword(s):  
Surgical Treatment ◽  
Hepatic Resection ◽  
Comparison Group ◽  
Postoperative Mortality ◽  
Late Results ◽  
Peripheral Cholangiocarcinoma ◽  
Group A ◽  
Comparative Group ◽  
Biliary Ducts ◽  
Clinically Significant

Objective. To estimate the immediate and late results of surgical treatment in patients, suffering peripheral cholangiocarcinoma with invasion of visceral vein. Маterials and methods. Retrospective analysis was accomplished for surgical treatment of 84 patients, suffering peripheral cholangiocarcinoma, in whom radical operations were performed in Department of Transplantation and Hepatic Surgery of Shalimov National Istitute of Surgery and Transplantology in a period from Jan. 2004 tо Dec. 2018 yrs. The investigated group consisted of 28 patients, to whom hepatic resection with simultant resection and plasty of visceral veins for tumoral vascular invasion was performed. Into comparison group 56 patients were included, in whom hepatic resection was not accompanied with vascular resection. Results. Trustworthy differences were absent between groups in accordance to following indices: the patients’ age (p=0.16-0.7), gender (p=0.3), physical status (p=0.36), pre- and postoperative stationary stay (p=0.4). In the investigated group there were performed 14.3% hemihepatectomies, 32.1% extended hemihepatectomies and 53.6% threesectioectomies, and in a comparative one - 57.2% (р ≤ 0.001), 14.3% (р = 0.054) and 21.4% (р = 0.002), accordingly. Simultant intervention on biliary ducts was done in 37.5% patients from investigated group and in 25% patients from comparative group (р = 0.305), the adjacent organs resection - in 14.3 and 12.5% (р = 0.819) patients, accordingly. Clinically significant postoperative complications were observed in 25 and 30.4% (р = 0.262) patients, accordingly. In the investigated group postoperative mortality was absent, while in a comparative one it constituted 3.5%. In the investigated group a 3-years and a 5-years total survival was noted in 47 and 35% patients, accordingly, while in a comparison group - in 49% (р = 0.317) and 38% (р = 0.003) patients, accordingly. In investigated group a 3-years and a 5-years survival without a recurrence was noted in 39 and 28% patients, accordingly, and in a comparative group - in 44% (р = 0.04) and 31% (р=0.002) patients, accordingly. Conclusion. Іnvasion of peripheral cholangiocarcinoma into visceral veins does not constitute a contraindication for operative treatment, if it is conducted in a highly specialized multidisciplinary centre.

Functional state of the liver in patients with dumping syndrome

1980 ◽  
Vol 61 (6) ◽  
pp. 37-39
Author(s):  
V. F. Bogoyavlenskiy ◽  
A. G. Oparin ◽  
R. M. Gazizov
Keyword(s):  
Blood Serum ◽  
Functional State ◽  
Early Identification ◽  
Serum Enzymes ◽  
Dumping Syndrome ◽  
Liver Lesions ◽  
Functional Tests

56 patients with dumping syndrome were examined. In 44 people, an increase in the activity of the isozymes LDH5,4, MDG3, ALP3, 1, CF1, AST2 was found to decrease the activity of the fourth fraction of esterases, while in 18 of them clinically, according to the results of widely used functional tests, no changes in the liver were detected. This gives grounds to recommend the study of the activity of hepatic isoenzymes LDH, MDH, ALP, CF, ACT esterases for early. identification of liver lesions in dumping syndrome in patients with resected patients; keywords: dumping syndrome, liver, blood serum enzymes.

scholarly journals Blood derived treatment from two allogeneic sources for severe dry eye associated to keratopathy: a multicentre randomised cross over clinical trial

2019 ◽  
Vol 104 (8) ◽  
pp. 1142-1147 ◽  
Author(s):  
Emilio Campos ◽  
Piera Versura ◽  
Marina Buzzi ◽  
Luigi Fontana ◽  
Giuseppe Giannaccare ◽  
...  
Keyword(s):  
Clinical Trial ◽  
Growth Factor ◽  
Blood Serum ◽  
Cord Blood ◽  
Dry Eye ◽  
Post Treatment ◽  
Subjective Symptoms ◽  
Treatment Change ◽  
Factor Content ◽  
Group A

AimTo compare the efficacy of cord blood and peripheral adult donor blood serum eyedrops, controlled for growth factor content, in the treatment of severe dry eye diseases (DED) resistant to conventional therapy.MethodsThis was a multicentre randomised, double-masked, cross-over clinical trial. Sixty patients diagnosed as severe DED, associated to persistent corneal epithelial defects were randomised and equally assigned to group A (treated with cord blood serum (CBS)) or group B (treated with PBS), eyedrops administered eight times/day for 1 month. Primary outcome was the pretreatment and post-treatment change in corneal fluorescein staining. Secondary outcomes included the pretreatment and post-treatment change in Ocular Surface Disease Index (OSDI) questionnaire and Visual Analogue Score (VAS) of subjective symptoms, Schirmer I test, tear break-up time and conjunctival staining. Patients with relapse in signs or symptoms after further 2 months switched to the remaining group for one additional month. Data were statistically analysed (p<0.05).ResultsCorneal staining was more significantly reduced after the CBS treatment, both VAS and OSDI score reduction was observed in both groups, but group A reported significantly less grittiness and pain. Nineteen patients shifted in the crossover period, the within individual comparison confirmed a better recovery in the CBS treatment period. Reduction in epithelial damage was positively associated with epidermal growth factor, transforming growth factorα and platelet-derived growth factor content. Levels of interleukins (IL-13) were positively associated with symptom decrease.ConclusionsOverall, DED signs improved after both CBS and PBS treatments, with potential advantages of CBS for subjective symptoms and corneal damage reduction.Clinical trial registrationNCT03064984.

Significance of isolated increases in total lactate dehydrogenase and its isoenzymes in serum of patients with bacterial pneumonia.

1988 ◽  
Vol 34 (7) ◽  
pp. 1503-1505 ◽  
Author(s):  
Z Rotenberg ◽  
I Weinberger ◽  
E Davidson ◽  
J Fuchs ◽  
O Sperling ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Lactate Dehydrogenase ◽  
Recovery Time ◽  
Bacterial Pneumonia ◽  
Normal Limit ◽  
Liver Function Tests ◽  
Total Serum ◽  
Group A ◽  
Group B ◽  
Group D

Abstract Total lactate dehydrogenase (LD, EC 1.1.1.27) activity in serum and LD isoenzymes were quantified at the time of diagnosis in 320 patients with bacterial pneumonia. In eighty, LD activity was increased, but this was accompanied by either other pathological results for liver-function tests or associated diseases that could explain it. The remaining 240 patients were divided into four groups, based on their total serum LD values: group A, less than 225 U/L (normal limit); group B, 226-350 U/L; group C, 351-499 U/L; and group D, greater than 500 U/L. Total LD was above normal at diagnosis in 40% of the patients. Recovery time was twice as long in group D as in groups A, B, and C. In five patients from group D, the pneumonia reflected underlying lung cancer. In groups B and C, the LD-3 ratio was increased in comparison with group A; in group D, LD-4 and LD-5 were increased up to twice the normal limit. Evidently nearly half of patients with bacterial pneumonia may show isolated increases in total LD activity (mostly LD-3) in serum. In cases with high activity, prolonged recovery time is expected. Intensive follow-up and extensive investigation are warranted in these patients, because some may have underlying lung cancer.

scholarly journals Blood Serum Enzymes Within the Estrous Cycle

1967 ◽  
Vol 50 (8) ◽  
pp. 1341-1342 ◽  
Author(s):  
J.D. Roussel ◽  
O.T. Stallcup
Keyword(s):  
Blood Serum ◽  
Estrous Cycle ◽  
Serum Enzymes

scholarly journals Evaluation by Monte Carlo Simulation of the Pharmacokinetics of Two Doses of Meropenem Administered Intermittently or as a Continuous Infusion in Healthy Volunteers

2005 ◽  
Vol 49 (5) ◽  
pp. 1881-1889 ◽  
Author(s):  
Wolfgang A. Krueger ◽  
Jurgen Bulitta ◽  
Martina Kinzig-Schippers ◽  
Cornelia Landersdorfer ◽  
Ulrike Holzgrabe ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Monte Carlo ◽  
Liquid Chromatography ◽  
Healthy Volunteers ◽  
Continuous Infusion ◽  
Intravenous Infusion ◽  
High Dose ◽  
Loading Dose ◽  
Group A ◽  
Group B

ABSTRACT Meropenem is a broad-spectrum carbapenem antibacterial agent. In order to optimize levels in plasma relative to the MICs, the ideal dose level and dosage regimen need to be determined. The pharmacokinetics of meropenem were studied in two groups, each comprising eight healthy volunteers who received the following doses: 500 mg as an intravenous infusion over 30 min three times a day (t.i.d.) versus a 250-mg loading dose followed by a 1,500 mg continuous infusion over 24 h for group A and 1,000 mg as an intravenous infusion over 30 min t.i.d. versus a 500-mg loading dose followed by a 3,000-mg continuous infusion over 24 h for group B. Meropenem concentrations in plasma and urine were determined by liquid chromatography-mass spectrometry/mass spectrometry and high-performance liquid chromatography with UV detection, respectively. Pharmacokinetic calculations were done by use of a two-compartment open model, and the data were extrapolated by Monte Carlo simulations for 10,000 simulated subjects for pharmacodynamic evaluation. There were no significant differences in total clearance and renal clearance between group A and group B or between the intermittent treatment and the continuous infusion. The analyses of the probability of target attainment by MIC for the high- and low-dose continuous infusions were robust up to MICs of 4 mg/liter and 2 mg/liter, respectively. The corresponding values for intermittent infusions were only 0.5 mg/liter and 0.25 mg/liter. When these observations were correlated with MICs obtained from the MYSTIC database, intermittent infusion results in adequate activity against two of the most common nosocomially acquired pathogens, Klebsiella pneumoniae and Enterobacter cloacae. However, against Pseudomonas aeruginosa, the evaluation shows a clear advantage of high-dose therapy administered as a continuous infusion. We believe that in the empirical therapy situation, the continuous-infusion mode of administration is most worth the extra efforts. We conclude that clinical trials for evaluation of the continuous infusions of meropenem in critically ill patients are warranted.

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