scholarly journals Hesperidin Increases Cytotoxic Effect of 5-Fluorouracil on WiDr Cells

2012 ◽  
Vol 3 (2) ◽  
pp. 404 ◽  
Author(s):  
Yurista Gilang ◽  
Adam Hermawan ◽  
Aditya Fitriasari ◽  
Riris Istighfari Jenie
Keyword(s):  
Colon Cancer ◽  
Cytotoxic Effect ◽  
Staining Method ◽  
Apoptosis Induction ◽  
Double Staining ◽  
Colon Cancer Cells ◽  
Cytotoxic Effects ◽  
Chemotherapy Agent ◽  
Ic50 Value ◽  
Double Staining Method

Therapy of colon cancer by using 5-FU often causes problems of resistance. This encourages the development of co-chemotherapy agent. One of the compounds that could potentially be used as a co-chemotherapy agent is hesperidin. This study was conducted to determine the cytotoxic effects of hesperidin, 5-FU and the combination of them, as well as apoptosis induction in colon cancer cells WiDr. Cytotoxic effect of hesperidin, 5-FU, and its combination were observed using MTT assay. Observation of apoptosis was done by double staining method using ethidium bromide-acridin orange. Until 48 hours incubation, hesperidin showed no cytotoxic effects. Cytotoxic effects of 5-FU was observed after 48 hours with the IC50 value of 422 µM. However, hesperidin improved the cytotoxic effects of 5-FU at 48 hour incubation. Either single treatment of hesperidin 200µM or 5-FU 1500 µM did not trigger apoptosis, but combination of them led to the emergence of signs of apoptosis. Based on this study,it can be concluded thathesperidin is potential to be developed as a co-chemotherapy agent of 5-FU on colon cancer but still need further study on its molecular mechanisms.Keywords : hesperidin, 5-fluorouracil, WiDr cells, cytotoxic, apoptosis

scholarly journals Cytotoxic Activity and Apoptosis Induction of Ethanolic Extract of Pericarps of Mangosteen (Garcinia mangostana Linn.) on WiDr Cells and Interaction Study of Alpha-mangosteen to IKK and VEGF Based on Molecular Docking

2013 ◽  
Vol 4 (1) ◽  
pp. 470
Author(s):  
Annishfia Lailatur Rohmah ◽  
Fikri Amalia ◽  
Erlina Rivanti ◽  
Dyaningtyas Dewi Pamungkas Putri ◽  
Nunuk Aries Nurulita
Keyword(s):  
Colon Cancer ◽  
Molecular Docking ◽  
Cancer Cells ◽  
Cytotoxic Effect ◽  
Ethanolic Extract ◽  
Apoptosis Induction ◽  
Colon Cancer Cells ◽  
Garcinia Mangostana ◽  
Double Staining Method ◽  
Native Ligand

One of the compounds found efficacious as an anti-proliferative on colon cancer is alpha-mangosteen, a xanthon compound that is abundant in pericarp of mangosteen. This study focused to evaluate anticancer activity of the ethanolic extract of pericarp of mangosteen (Garcinia mangostana Linn.) on WiDr colon cancer cells and to observe the affinity of alpha-mangosteen in inhibiting IKK and VEGF. Cytotoxic effect was tested by MTT assay and apoptosis induction was evaluated by double staining method on WiDr colon cancer cells, while interaction between alpha-mangosteen to the receptors was measured by molecular docking. The ethanolic extract was proven to have cytotoxic activity against WiDr colon cancer cells with IC50 of 25 µg/mL. In addition, the observation of apoptosis induction by double-staining method showed that apoptosis associated the cytotoxic effect of ethanolic extract of pericarp of mangosteen (EPM) on WiDr colon cancer cells. Molecular docking showed that active compounds in pericarp of mangosteen could compete with the native ligand of VEGF because the docking score of alpha-mangosteen was almost equal with native ligand. From these results we could be concluded that the cytotoxic effect of EPM to WiDr cells was mediated by cell apoptosis. This extract was potential to be developed as chemopreventive agent.Keywords : Garcinia mangostana Linn., cytotoxic effect, apoptosis, WiDr cell, molecular docking 

Enzyme histochemical application and backscattered electron imaging to visualize the distribution of lymphatic capillaries

1990 ◽  
Vol 48 (3) ◽  
pp. 880-881
Author(s):  
Seiji Kato
Keyword(s):  
Staining Method ◽  
Double Staining ◽  
Reaction Products ◽  
Blood Capillaries ◽  
Backscattered Electron Imaging ◽  
Histochemical Observation ◽  
Backscattered Electron ◽  
Double Staining Method ◽  
Electron Imaging ◽  
Cryostat Sections

Previously, the author repeatedly confirmed the higher 5’-nucleotidase (5’-Nase) and lower alkaline phoaphatase (ALPase) activities in the wall of lymphatic capillaries reacted with the lead-based method relative to those of blood capillaries. The ALPase, on the other hand, is markedly higher in blood capillaries than in lymphatics. On the basis of these enzyme characteristics, the author has developed a 5’-Nase— ALPase double staining method to differentiate small lymphatics from blood capillaries at the level of the light microcsopy. Furthermore, we applied it to histochemical observation of the lead-containing reaction products of 5’-Nase in lymphatics on the same or adjacent cryostat sections using backscattered electron imaging (BEI) in scanning electron microscope (SEM). This paper presents a new applicability of 5’-Nase histochemistry by BEI-SEM to demonstrate the distribution of lymphatic capillaries in tissue blocks.

scholarly journals Immunohistochemical/histochemical double staining method in the study of the columnar metaplasia of the oesophagus

2014 ◽  
Vol 58 (1) ◽  
Author(s):  
D. Cabibi ◽  
A.G. Giannone ◽  
C. Mascarella ◽  
C. Guarnotta ◽  
M. Castiglia ◽  
...  
Keyword(s):  
Staining Method ◽  
Double Staining ◽  
Columnar Metaplasia ◽  
Double Staining Method

Apoptotic and cytotoxic effect of bioactive fractions of Rhus trilobata on colon cancer cells CaCo-2

2016 ◽  
Vol 259 ◽  
pp. S193-S194
Author(s):  
L.E. Híjar-Soto ◽  
C. González-Horta ◽  
D. Chávez-Flores ◽  
B. Sánchez-Ramírez
Keyword(s):  
Colon Cancer ◽  
Cancer Cells ◽  
Cytotoxic Effect ◽  
Colon Cancer Cells

scholarly journals Phaleria macrocarpa Fruit Extract Inhibits NF-ĸB Activation and Apoptosis Induction on HeLa Cells

2017 ◽  
Vol 8 (1) ◽  
pp. 9
Author(s):  
Meirizky Zulharini S. ◽  
Amalia Miranda ◽  
Lina Permatasari ◽  
Hilyatul Fadliyah ◽  
Riris Istighfari Jenie
Keyword(s):  
Transcription Factor ◽  
Molecular Docking ◽  
Mtt Assay ◽  
Apoptosis Induction ◽  
Double Staining ◽  
Fruit Extract ◽  
Apoptosis Inhibition ◽  
Ic50 Value ◽  
Anticancer Mechanism ◽  
Phaleria Macrocarpa

NF-κB is a transcription factor and if activated, it induces apoptosis inhibition. Phalerin from Phaleria macrocarpa fruits expected to inhibit NF-κB activation. This research is to investigate anticancer mechanism of Phaleria macrocarpa fruits extracts (EBMD) in NF-κB pathway. Molecular docking assay was performed to determine phalerin affinity to IKK. Cytotoxic activity was observed by MTT assay. Double staining was performed to determine the apoptotic cells. Docking score of phalerin to IKK is -60. The IC50 value of EBMD is 629 μg/mL. Apoptosis profile shows (shown that) many cells undergoing apoptosis after treatment. Thus, EBMD potentially inhibits activation of NF-κB pathway and triggers apoptosis on HeLa cells.Keywords : NF-κB, Phaleria macrocarpa, sel HeLa, Bcl-2, IKK, molecular docking

The Flavonol Isorhamnetin Exhibits Cytotoxic Effects on Human Colon Cancer Cells

2010 ◽  
Vol 58 (20) ◽  
pp. 10869-10875 ◽  
Author(s):  
Sara Jaramillo ◽  
Sergio Lopez ◽  
Lourdes M. Varela ◽  
Rocio Rodriguez-Arcos ◽  
Ana Jimenez ◽  
...  
Keyword(s):  
Colon Cancer ◽  
Cancer Cells ◽  
Human Colon ◽  
Colon Cancer Cells ◽  
Human Colon Cancer ◽  
Cytotoxic Effects ◽  
Human Colon Cancer Cells
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