scholarly journals Obtaining lactic acid through discontinuing fermentation using sugarcane molasses, cassava by-product meal, fructose and cassava starch

2010 ◽  
Vol 1 (2) ◽  
pp. 97
Author(s):  
Milena Veronezi Silva Silva ◽  
Odinei Hess Gonçalves ◽  
Mirela Vanin dos Santos Lima ◽  
Heron Oliveira dos Santos Lima

<p>The lactic acid presents its larger application in the Food Industry. It is also used in the Textile, Pharmaceutical, Chemical, Cosmetic and Packaging Industries. Due to this wide applicability, the acquisition of lactic acid through fermentation is a widely studied process, searching for alternatives for the increase in productivity and the decrease in the production costs. Therefore, this work has the purpose of evaluating the influence of different types of cultivation, proceeding from the agro-industry, regarding the production of lactic acid through fermentation, using <em>Lactobacillus casei</em>. In order to do that, it was used as the cultivation media, sugarcane molasses, cassava by-product crumbs, fructose and cassava starch, previously hydrolyzed when necessary and supplemented with 2% yeast extract and 2% peptone. Fermentations were performed at 37&ordm; for 48 hours under constant agitation. The fermentation processes were followed by analysis such as: lactic acid dosage; reductive sugar decrease; biomass; cellular viability and pH. When the results were analyzed it was noticed a larger decrease of the reductive sugar and a larger production of lactic acid when the sugarcane molasses were used as cultivation media. Therein it may be suggested that the hydrolyzed sugarcane molasses presented itself more efficient in promoting the microorganism growth. Such result has also been observed by other authors in the researched literature and it can be explained by the fact that the hydrolyzed sugarcane molasses presents in its composition, besides glucose and fructose, other nutrients able to favor the growth of the microorganism and, consequently, the production of the lactic acid when compared to other medias used in this work.</p><p>&nbsp;</p><p>DOI: http://dx.doi.org/10.14685/rebrapa.v1i2.23</p>

2021 ◽  
Author(s):  
Song Wang ◽  
Ran Tian ◽  
Buwei Liu ◽  
Hongcai Wang ◽  
Jun Liu ◽  
...  

Abstract Sugarcane molasses are considered a potential source for bioethanol's commercial production because of its availability and low market price. It contains high concentrations of fermentable sugars that can be directly metabolized by microbial Fermentation. Lactic acid bacteria, especially Lactobacillus casei, have a high potential to be a biocatalyst in ethanol production that they are characterized by strong abilities of carbohydrate metabolism, ethanol synthesis, and high alcohol tolerance. This study aimed to evaluate the feasibility of producing ethanol from sugarcane molasses by Lactobacillus casei used engineering strain L. casei E1 as a starter culture. The effects of environmental factors on the metabolism of L. casei E1 were analyzed by high-performance liquid chromatography (HPLC) system, and the gene expression of key enzymes in carbon source metabolism was detected using quantitative real-time PCR (RT-qPCR). Results showed that the strain could grow well, ferment sugar quickly and produce high yielded ethanol in cane molasses. By fermenting this bacterium anaerobically at 37°C for 36 h incubation in 5 °BX molasses when the fermenter's pH was controlled at 6.0, ethanol yield reached 13.77 g/L, and carbohydrate utilization percentage was 78.60%. RT-qPCR results verified the strain preferentially ferment glucose and fructose of molasses to ethanol at the molecular level. In addition, the metabolism of sugars, especially fructose, would be inhibited by elevating acidity. Our findings support the theoretical basis for exploring Lactic acid bacteria as a starter culture for converting sugarcane molasses into ethanol.


2014 ◽  
Vol 32 (No. 6) ◽  
pp. 526-531 ◽  
Author(s):  
Š. Horáčková ◽  
P. Sedláčková ◽  
M. Sluková ◽  
M. Plocková

The effect of whey powder, whey protein concentrate, caseinomacropeptide, and malt addition into milk on the growth and acid production of lactobacilli (Lactobacillus casei Lafti L-26, Lactobacillus acidophilus CCDM 151, and Lactobacillus casei CCDM 198) was evaluated. The ability of these strains to use different types of saccharides from milk and plant sources was also tested. Glucose, galactose, fructose and maltose were utilised by all tested strains. The results showed that the addition of malt positively affected the growth of lactobacilli strains compared to the growth in milk enriched by whey ingredients. The addition of malt increased significantly the production of d(&ndash;)isomer of lactic acid by Lactobacillus acidophilus CCDM 151 and Lactobacillus casei CCDM 198 and the production of acetic acid by Lactobacillus casei CCDM 198. &nbsp;


2021 ◽  
Vol 2021 ◽  
pp. 1-9
Author(s):  
Haojue Wang ◽  
Zichen Cui ◽  
Xuan Wang ◽  
Shui Sun ◽  
Dongsheng Zhang ◽  
...  

Since the discovery of horseradish peroxidase-like activity of magnetite nanoparticles in 2007, many researchers have investigated different types of nanoparticles that show enzyme-like activities, namely, nanozymes. Nanozymes possess high efficiency, stability, and low production costs compared to natural enzymes. Thus, nanozymes have already been widely studied in various domains including medical science, food industry, chemical engineering, and agriculture. This review presents the utilization of nanozymes in medicine and focuses particularly on their therapeutic applications in chronic inflammatory diseases because of their antioxidant-like activity. Furthermore, the treatment of chronic inflammatory diseases with nanozymes of different materials was introduced emphatically.


Revista Vitae ◽  
2020 ◽  
Vol 27 (1) ◽  
Author(s):  
Laura Bolivar Parra ◽  
Paula Andrea Giraldo Hincapié ◽  
Olga Inés Montoya Campuzano

Background: Lactic Acid Bacteria (LAB) are of special interest in the food industry due to their ability to produce metabolites. Among them, bacteriocins, which can inhibit the growth of altering microorganisms, and pathogens in a wide variety of foods, are considered safe for human consumption and are used as preservatives. Objectives: Evaluate the effect of a bacteriocin found by in silico methods on the microbiota present in Antioquian soft cheese. Methods: In this research, we design a synthetic bacteriocin, called Bac 22, found in the genome of Lactobacillus casei using the genomic mining methodology and bioinformatics tools. We also conducted a preliminary biological and hemolytic activities studies of the Bac 22 toward the microbiota present in the Antioquian soft cheese (Quesito Antioqueño). Results: The bacteriocin Bac 22 at a concentration of 100 μM presented a hemolytic capacity lower than 1% and reduced the CFU / g of total coliforms significantly when added to Antioquian soft cheese for eight days. Conclusions: The Bac 22 demonstrated a positive potential effect over the shelf life of a dairy product, such as the Antioquian soft cheese.


2010 ◽  
Vol 1 (1) ◽  
pp. 11
Author(s):  
Roselene Ferreira Oliveira ◽  
Heron Oliveira dos Santos Lima ◽  
Mirela Vanin dos Santos Lima

<p>Lactic acid has multiple uses in several industries such as food, cereal derivates, beverage, cosmetic, chemical and pharmaceutical. Due to its wide applicability the process to obtain lactic acid is one of the most studied processes. The aim of this study was to produce lactic acid using fermentation of cassava meal (residue from cleaning the flour mill) and cassava starch (amilacious fractions of tuberous root raw materials) previously hydrolyzed and supplemented. The fermentation of both cassava meal and cassava starch was carried out using a solution at 18% (m/v), previously hydrolyzed with thermostable alpha amylase (Termamyl 120L) and amyloglucosidase (AMG 300L); supplemented with yeast extract and peptone. The microorganism, Lactobacillus casei, was inoculated under the following process conditions: pH 6.4; at 37&deg;C and agitation at 100 rpm for 96 hours. The process was periodically surveyed in order to analyze the concentration of lactic acid; concentration of reducing sugars; pH; biomass and cellular feasibility. The analysis of the results permits to conclude that both cassava meal and cassava starch are promising raw materials for obtaining lactic acid by fermentative media.</p><p>&nbsp;</p><p><span>DOI:&nbsp;<a href="http://dx.doi.org/10.14685/rebrapa.v4i1.102">http://dx.doi.org/10.14685/rebrapa.v1i1.2</a></span></p><p>&nbsp;</p>


2020 ◽  
Vol 2 (1) ◽  
Author(s):  
Nurul Mukminah ◽  
Rita Purwasih

This study aims to determine and compare the profitability of different types of broiler chicken farms (open and cloused houses) in Subang Regency. This study involved 9 farmers consisting of 5 farmers with closed house and 4 farmers with open house who partnered with PT. Surya Unggas Mandiri (PT. SUM). Production costs taken are 2 production periods from May-August 2018. Data are analyzed using economic analysis and descriptive methods. The results showed that the production cost per period per 1000 birds closed house was higher (Rp. 27.656.768,-) compared to open house (Rp. 24.975.671,-). The revenue per period per 1000 birds of closed house is higher (Rp. 30.606.931,-) compared to open house (Rp. 25.788.618,-). The profit of farmers who use closed house is higher (Rp. 2.621/period/bird) than open house (Rp.417,-). The profitability in close house s is 9.48% and is very feasible to develop


2020 ◽  
Vol 70 (12) ◽  
pp. 4594-4600

The purpose of this study was to characterize some types of biomass wastes resulted from different activities such as: agriculture, forestry and food industry using thermogravimetric and ICP-MS analyses. Also, it was optimized an ICP-MS method for the determination of As, Cd and Pb from biomass ash samples. The ICP-MS analysis revealed that the highest concentration of metals (As, Cd, Pb) was recorded in the wood waste ash sample, also the thermogravimetric analysis indicated that the highest amount of ash was obtained for the same sample (26.82%). The biomass wastes mentioned in this study are alternative recyclable materials, reusable as pellets and briquettes. Keywords: ash, biomass, ICP-MS, minor elements, TG


2019 ◽  
Vol 25 (24) ◽  
pp. 2661-2676 ◽  
Author(s):  
Sundaresan Bhavaniramya ◽  
Ramar Vanajothi ◽  
Selvaraju Vishnupriya ◽  
Kumpati Premkumar ◽  
Mohammad S. Al-Aboody ◽  
...  

Enzymes exhibit a great catalytic activity for several physiological processes. Utilization of immobilized enzymes has a great potential in several food industries due to their excellent functional properties, simple processing and cost effectiveness during the past decades. Though they have several applications, they still exhibit some challenges. To overcome the challenges, nanoparticles with their unique physicochemical properties act as very attractive carriers for enzyme immobilization. The enzyme immobilization method is not only widely used in the food industry but is also a component methodology in the pharmaceutical industry. Compared to the free enzymes, immobilized forms are more robust and resistant to environmental changes. In this method, the mobility of enzymes is artificially restricted to changing their structure and properties. Due to their sensitive nature, the classical immobilization methods are still limited as a result of the reduction of enzyme activity. In order to improve the enzyme activity and their properties, nanomaterials are used as a carrier for enzyme immobilization. Recently, much attention has been directed towards the research on the potentiality of the immobilized enzymes in the food industry. Hence, the present review emphasizes the different types of immobilization methods that is presently used in the food industry and other applications. Various types of nanomaterials such as nanofibers, nanoflowers and magnetic nanoparticles are significantly used as a support material in the immobilization methods. However, several numbers of immobilized enzymes are used in the food industries to improve the processing methods which not only reduce the production cost but also the effluents from the industry.


Polymers ◽  
2021 ◽  
Vol 13 (11) ◽  
pp. 1811
Author(s):  
Valeriia Rostovtseva ◽  
Alexandra Pulyalina ◽  
Roman Dubovenko ◽  
Ilya Faykov ◽  
Kseniya Subbotina ◽  
...  

Modification of polymer matrix by hybrid fillers is a promising way to produce membranes with excellent separation efficiency due to variations in membrane structure. High-performance membranes for the pervaporation dehydration were produced by modifying poly(2,6-dimethyl-1,4-phenylene oxide) (PPO) to facilitate lactic acid purification. Ionic liquid (IL), heteroarm star macromolecules (HSM), and their combination (IL:HSM) were employed as additives to the polymer matrix. The composition and structure of hybrid membranes were characterized by X-ray diffraction and FTIR spectroscopy. Scanning electron microscopy was used to investigate the membranes surface and cross-section morphology. It was established that the inclusion of modifiers in the polymer matrix leads to the change of membrane structure. The influence of IL:HSM was also studied via sorption experiments and pervaporation of water‒lactic acid mixtures. Lactic acid is an essential compound in many industries, including food, pharmaceutical, chemical, while the recovering and purifying account for approximately 50% of its production cost. It was found that the membranes selectively remove water from the feed. Quantum mechanical calculations determine the favorable interactions between various membrane components and the liquid mixture. With IL:HSM addition, the separation factor and performance in lactic acid dehydration were improved compared with pure polymer membrane. The best performance was found for (HSM: IL)-PPO/UPM composite membrane, where the permeate flux and the separation factor of about 0.06 kg m−2 h−1 and 749, respectively, were obtained. The research results demonstrated that ionic liquids in combination with star macromolecules for membrane modification could be a promising approach for membrane design.


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