scholarly journals The signal axis GATA2-EDN1-AGT induced hypertension from obstructive sleep apnea-hypopnea syndrome with the clinical and animal study

2022 ◽  
Vol 67 (4) ◽  
pp. 91-96
Author(s):  
Li Zhang ◽  
Shaokun Xu ◽  
Ning Zhou ◽  
Juanqin Shen ◽  
Jiaqing Huang ◽  
...  
Keyword(s):  
Obstructive Sleep Apnea ◽  
Sleep Apnea ◽  
Systolic Pressure ◽  
Strong Relationship ◽  
Enzyme Linked Immunosorbent Assay ◽  
Control Group ◽  
Case Group ◽  
Obstructive Sleep ◽  
Induced Hypertension ◽  
Hypopnea Syndrome

Hypertension occurred in 50% obstructive sleep apnea-hypopnea syndrome (OSAHS) patients meanwhile OSAHS occurred in 30% hypertension patients. The present study aimed to explore the molecular mechanism of GATA2-EDN1-AGT induced hypertension in the development of obstructive sleep apnea-hypopnea syndrome. OSAHS patients (56 cases: 36 cases of male, 20 cases of female, 42~60 years old) were divided into two groups (case group: patients with hypertension monitored by 24 h ambulatory blood pressure and polysomnography; control group: patients without hypertension). Wistar rats were used to establish the OSAHS model (narrow pharyngeal cavity). PaO2 and PaCO2 of patients and rats were measured by an automatic blood gas analyzer. The profile of total protein in the OSAHS group and normal group was evaluated. Protein-protein-interaction (PPI) was carried out to show all matter proteins related. The levels of EDN-1, AGTII and atrial natriuretic peptide (ANP) in blood samples of patients and rats were analyzed by enzyme-linked immunosorbent assay (ELISA). The expression of GATA2, EDN1, endothelin-converting enzyme 1 (ECE-1) and AGTⅡ was measured. The results showed that SaO2 and AHI were positively associated with systolic pressure (P<0.05) in OSAHS patients. There was no correlation among other indexes (P>0.05). It was also observed that GATA2 had a strong relationship with AGTⅡ and EDN1. The results of ELISA presented that the levels of EDN1, AGTⅡ and ANP in the OSAHS group of human and animal models were significantly increased (P<0.05). The results of immunochemistry showed that the expression of GATA2 and AGTⅡ in the vascular of OSAHS group was upregulated manifestly (P<0.05). It was concluded that OSAHS can induce AHI, which increases hypertension via the GATA2-EDN1-AGT Ⅱ axis.

scholarly journals Irisin Is Correlated with Blood Pressure in Obstructive Sleep Apnea Patients

2021 ◽  
Vol 2021 ◽  
pp. 1-9
Author(s):  
Xing Wang ◽  
Zhengjiao Zhang ◽  
Xiaoxin Lan ◽  
Keyou Fu ◽  
Guanhua Xu ◽  
...  
Keyword(s):  
Blood Pressure ◽  
Obstructive Sleep Apnea ◽  
Sleep Apnea ◽  
Secondary Hypertension ◽  
The Other ◽  
Enzyme Linked Immunosorbent Assay ◽  
Control Group ◽  
Apnea Hypopnea Index ◽  
Potential Biomarker ◽  
Obstructive Sleep

Background. Despite approximately 95% primary cases of hypertension, secondary hypertension seems to be common with resistant forms. Notably, obstructive sleep apnea (OSA) is known as a common cause of secondary hypertension and has a major characteristic of obesity. Irisin acts as a link between muscles and adipose tissues in obesity, playing an essential role in human blood pressure (BP) regulation. However, whether irisin is associated with secondary hypertension caused by OSA and how it takes effect essentially have not been elucidated. Purpose. To investigate the changes of irisin and its relationship with BP in OSA. Methods. 72 snoring patients finished Epworth Sleep Scale (ESS) evaluation before polysomnography (PSG). BP was the average of three brachial BP values by mercury sphygmomanometer. Serum irisin level was determined by enzyme-linked immunosorbent assay (ELISA). Results were analyzed by SPSS software. Results. Irisin was higher in the severe and quite severe group than that in control and nonsevere groups ( p < 0.05 ). For BP, significant differences were found between the control group and the other three groups ( p < 0.05 ) and between the quite severe and the other three groups ( p ≤ 0.001 ). Positive correlations were found between irisin and apnea-hypopnea index (AHI), AHI and BP, and irisin level and BP. Negative correlations were between irisin and SpO2 nadir and SpO2 nadir and BP. Positive correlation still existed between AHI and irisin even after adjusting for some obesity-related variables. Conclusions. Irisin may serve as a potential biomarker for severity of OSA independently of obesity and imply the development of hypertension.

scholarly journals Novel proteins associated with chronic intermittent hypoxia and obstructive sleep apnea: From rat model to clinical evidence

PLoS ONE ◽  
2021 ◽  
Vol 16 (6) ◽  
pp. e0253943
Author(s):  
Xiaojun Tang ◽  
Shisheng Li ◽  
Xinming Yang ◽  
Qinglai Tang ◽  
Ying Zhang ◽  
...  
Keyword(s):  
Obstructive Sleep Apnea ◽  
Sleep Apnea ◽  
Rat Model ◽  
Intermittent Hypoxia ◽  
Mapk Signaling ◽  
Enzyme Linked Immunosorbent Assay ◽  
Chronic Intermittent Hypoxia ◽  
Control Group ◽  
Obstructive Sleep ◽  
And Control

Objective To screen for obstructive sleep apnea (OSA) biomarkers, isobaric tags for relative and absolute quantitation (iTRAQ)-labeled quantitative proteomics assay was used to identify differentially expressed proteins (DEPs) during chronic intermittent hypoxia (CIH). Method The iTRAQ technique was applied to compare DEPs in the serum of a CIH rat model and control group. Biological analysis of DEPs was performed using Gene Ontology and Kyoto Encyclopedia to explore related biological functions and signaling pathways. Enzyme-linked immunosorbent assay (ELISA) was performed to validate their expression in sera from patients with OSA and CIH rats. Results Twenty-three DEPs (fold change ≥1.2 or ≤0.833, p<0.05) were identified, and two DEPs (unique peptides>3 and higher coverage) were further verified by ELISA in the CIH rat model and OSA subject: apolipoprotein A-IV (APOA4, p<0.05) and Tubulin alpha-1A chain (TUBA1A, p<0.05). Both groups showed significant differences in the expression levels of DEPs between the CIH and control groups and the severe OSA and non-OSA groups. APOA4 was found to be upregulated and TUBA1A downregulated in both the sera from OSA patients and CIH rats, on comparing proteomics results with clinical results. There were two pathways that involved three DEPs, the mitogen-activated protein kinase (MAPK) signaling pathway (p<0.05) and cytokine-cytokine receptor interaction (p<0.05). Conclusion APOA4 and TUBA1A may be potential novel biomarkers for CIH and OSA, and may play an important role in the development of OSA complications.

scholarly journals Obstructive sleep apnea-hypopnea syndrome patients prone to developing anisometropia

2019 ◽  
Author(s):  
Hua-Xuan Zhao ◽  
Ying-Ying Gao ◽  
Xiao-Yang Chen ◽  
Yi-Ming Zeng
Keyword(s):  
Obstructive Sleep Apnea ◽  
Sleep Apnea ◽  
Asymmetry Index ◽  
Control Group ◽  
Positional Obstructive Sleep Apnea ◽  
Regularity Index ◽  
Obstructive Sleep ◽  
Contralateral Eye ◽  
Hypopnea Syndrome ◽  
Surface Regularity

Abstract Objective: This study aims to investigate anisometropia in obstructive sleep apnea-hypopnea syndrome patients. Methods: The obstructive sleep apnea-hypopnea syndrome cohort was divided into groups according to sleeping position: positional obstructive sleep apnea-hypopnea syndrome group (OSA-S group) (n=32), subjects who slept on their sides, and non-positional obstructive sleep apnea-hypopnea syndrome group (OSA-NS group) (n =29), subjects who did not sleep on their sides. The incidence of anisometropia, values of corneal topography, and ocular axial length were compared between groups, and the incidence of anisometropia at different degrees of obstructive sleep apnea-hypopnea syndrome severity was analyzed. Results: The obstructive sleep apnea-hypopnea syndrome groups had a significantly higher incidence of anisometropia than the control group. The OSA-S group had a significantly higher incidence of anisometropia compared with the other groups. There was a significant difference in the incidence of anisometropia between the two obstructive sleep apnea-hypopnea syndrome subgroups with different degrees of severity. The obstructive sleep apnea-hypopnea syndrome groups had significantly higher surface regularity index, surface asymmetry index and cylinder values than the control group. In the OSA-S group, the surface regularity index, surface asymmetry index and cylinder of the eye on the preferred sleeping side were significantly higher than those in the contralateral eye. The ocular axial length of the eye was significantly greater on the preferred sleeping side than in the contralateral eye in the 22 patients with anisometropia in the OSA-S group (P<0.05). Conclusions: obstructive sleep apnea-hypopnea syndrome patients who usually sleep on one side are prone to developing anisometropia, and the incidence of anisometropia is associated with the severity of obstructive sleep apnea-hypopnea syndrome.

scholarly journals Serum Uric Acid Is Independently Associated with Risk of Obstructive Sleep Apnea-Hypopnea Syndrome in Chinese Patients with Type 2 Diabetes

2019 ◽  
Vol 2019 ◽  
pp. 1-6 ◽  
Author(s):  
Caiyu Zheng ◽  
Haiqu Song ◽  
Shunhua Wang ◽  
Jing Liu ◽  
Tingting Lin ◽  
...  
Keyword(s):  
Type 2 Diabetes ◽  
Obstructive Sleep Apnea ◽  
Uric Acid ◽  
Sleep Apnea ◽  
Serum Uric Acid ◽  
Control Group ◽  
Apnea Hypopnea Index ◽  
Obstructive Sleep ◽  
Hypopnea Syndrome

Purpose. We aimed to investigate the association between serum uric acid (SUA) levels and obstructive sleep apnea-hypopnea syndrome (OSAHS) in patients with type 2 diabetes. Methods. A cross-sectional study of 212 type 2 diabetes mellitus (T2DM) patients was conducted in Xiamen, China. All patients underwent polysomnography (PSG) recordings for OSAHS diagnosis. Patients were grouped according to the apnea-hypopnea index (AHI) as mild (5-14.9), moderate (15-29.9), and severe (≧30) OSAHS. Patients with AHI≤4.9 served as the control group. Weight, body mass index (BMI), SUA, liver function, renal function, blood pressure, lipid profiles, and glycemic parameters were measured. Results. A total of 158 patients (101 men and 57 women) with complete data were analyzed in this study. 127 patients were identified as OSAHS. Among the 127 patients with OSAHS, 56 (44.1%), 37 (29.1%), and 34 (26.8%) had mild, moderate, and severe OSAHS, respectively. Correlation analyses showed that the SUA level was significantly related to the apnea-hypopnea index (AHI) (r=0.194, p=0.016). The level of SUA was significantly higher among OSAHS patients compared to the control group (control group: 333.14±80.52 μmol/L, mild group: 345.50±90.27 μmol/L, moderate group: 363.59±134.26 μmol/L, and severe group: 428.37±123.58 μmol/L and p=0.029). Multivariable logistic regression analyses showed that SUA was the independent risk factor for OSAHS (OR: 1.006, 95% CI: 1.001-1.011, p=0.020). Conclusions. The SUA level is significantly associated with the severity of OSAHS and should be controlled when managing OSAHS.

scholarly journals Comparing Cognitive Performance in Patients with Obstructive Sleep Apnea and healthy Subjects

2018 ◽  
Author(s):  
Maryam Maghsoudipour ◽  
Shoaleh Memari ◽  
Khosro Sadeghniiat Haghighi ◽  
Pourya Rezasoltani
Keyword(s):  
Obstructive Sleep Apnea ◽  
Sleep Apnea ◽  
Cognitive Performance ◽  
Healthy Subjects ◽  
Pearson Correlation ◽  
Direct Consequence ◽  
Control Group ◽  
Case Group ◽  
Obstructive Sleep ◽  
Significant Difference

Introduction::   Daily performance deficits are inevitable results of a large group of sleep disorders. Cognition and cognitive performance might be defected as a direct consequence of sleep disorder assessed by subjective or objective measures. So, this survey was performed in order to compare cognitive performance of obstructive sleep apnea patients and healthy subjects in Baharloo hospital of Tehran.   Method:  In this case control study, the samples were chosen by  convenience sampling, from Baharloo hospital patients and were assigned to case group (apnea patients) and control group (healthy subjects), based on AHI score. Also, we used a demographic questionnaire, ESS, Berlin, and STOP BANG. Appropriate tool was used to assess cognitive performance in two groups. In order to analyze data we used SPSS (version 19).  Independent T test, Chi square, Fisher test, Kruskal-wallis Test, and Pearson correlation test were used. Results:   Results demonstrated a significant difference of sleepiness and cognitive performance between healthy subjects and OSA (Obstructive Sleep Apnea) patients.  Conclusion:   Screening and diagnosis of OSA patients, and considering the deficient cognitive performance is crucial. Also, education about the importance of OSA Syndrome and the treatment is necessary.     Keywords:  Obstructive sleep apnea, Cognitive performance, Excessive daytime sleepiness

2 The association between obstructive sleep apnea severity and cognition in older people

2020 ◽  
Vol 91 (8) ◽  
pp. e9.1-e9
Author(s):  
Yu-Jun Luo ◽  
Chen-Chen Lo ◽  
Cheng-Yu Tsai ◽  
Yin-Tzu Lin ◽  
Hsin-Mei Hsu ◽  
...  
Keyword(s):  
Obstructive Sleep Apnea ◽  
Sleep Apnea ◽  
Sleep Apnea Syndrome ◽  
Sleep Efficiency ◽  
Lower Score ◽  
Control Group ◽  
Case Group ◽  
Time In Bed ◽  
Obstructive Sleep ◽  
Four Levels

Relationship between obstructive sleep apnea syndrome (OSAS) and dementia has been investigated in recent years. However, the effects of hypoxia during sleep timeon dementia remain unclear. The objective of this study is to associate the score of mini-mental state examination (MMSE) with OSAS. In this study, 67 patients whom were more than 50 years old and have been done for MMSE test and diagnosed with OSAS in sleep center in Taipei from 2015 to 2018. Four levels of OSAS severity were classified by the apnea and hypopnea index (AHI), according to the American Academy of Sleep Medicine (AASM) guideline. Subjects with MMSE scores above 25 and below 25 were served respectively as case group and control group. Logistic regression analyses were used to examine the associations. The average AHI were 28.48 times per hour for case group and 17.93 times per hour for control group. The AHI had a 1.03 fold higher odds ratio (95% CI=0.99 ~ 1.07, p=0.06) for lower score group compared with higher score group. The mean sleep efficiency were 57.8% in lower score group, while higher score group had an average sleep efficiency of 70.3%. Furthermore, sleep efficiency, total sleep time, the ratio of wake to total time in bed as well as the ratio of wake to sleep period time in case group were significant lower than the control group. We observe the associations between MMSE score and some parameters of polysomnography. The subjects with high severity OSAS, insufficient sleep efficacy, longer awake duration and shorter sleep duration could lead to cognitive impairment.

scholarly journals Circadian Melatonin Secretion In Obese Adolescents With Or Without Obstructive Sleep Apnea

2020 ◽  
Vol 9 (4) ◽  
Author(s):  
Olga N. Berdina ◽  
Irina M. Madaeva ◽  
Svetlana E. Bolshakova ◽  
Maria V. Tsykunova ◽  
Leonid F. Sholokhov ◽  
...  
Keyword(s):  
Obstructive Sleep Apnea ◽  
Sleep Apnea ◽  
Clinical Manifestations ◽  
Enzyme Linked Immunosorbent Assay ◽  
Control Group ◽  
Melatonin Level ◽  
Melatonin Secretion ◽  
Blood Oxygen Saturation ◽  
Obese Adolescents ◽  
Obstructive Sleep

Objective — To compare melatonin levels in saliva during a 24-hr day in order to identify the specificities of circadian melatonin secretion in obese adolescents with or without obstructive sleep apnea (OSA). Material and Methods — We examined 18 obese adolescents with OSA, 12 obese adolescents without OSA, and 15 healthy adolescents with a normal body weight, from whom saliva was sampled four time during the 24-hr day. Polysomnography was used to diagnose OSA. Saliva samples (n=180) were subjected to enzyme-linked immunosorbent assay. Results — Obese adolescents with OSA had higher evening melatonin levels than obese adolescents without OSA. For example, this indicator in OSA patients was 5.3 times higher than in participants without OSA, who had the lowest evening melatonin level among all groups. In both obese groups, nighttime melatonin levels were significantly lower than in the control group. A positive correlation was detected between the levels of morning and afternoon melatonin and body mass index only in obese adolescents without OSA (r=0.58; p=0.03 and r=0.68; p=0.01, respectively). It was found that evening melatonin correlated with minimum blood oxygen saturation (SaO2) in the entire sample of adolescents with OSA (r=-0.69; p=0.008), and it also correlated with time with SaO2 <90% in the group with clinical manifestations of OSA (r=0.76; p=0.003). Nighttime melatonin levels negatively correlated with the minimum SaO2 value solely in the group with clinical manifestations of OSA (r=-0.58; p=0.035). Conclusion — The circadian melatonin secretion in obese adolescents differed, depending on the presence or absence of OSA, and correlated with the level of oxygen desaturation in OSA patients, to a greater extent – in the presence of clinical manifestations.

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