Osteoblast Patterning on Silicone Rubber by using Mesenchymal Stem Cells and Carbon Negative-Ion Implantation

The objective of the work was to study the effect of high-dose ion implantation (HDII) of NiTi surface layers with Si Ti, or Zr, on the NiTi biocompatibility. The biocompatibility was judged from the intensity and peculiarities of proliferation of mesenchymal stem cells (MSCs) on the NiTi specimen surfaces treated by special mechanical, electrochemical, and HDII methods and differing in chemical composition, morphology, and roughness. It is shown that the ion-implanted NiTi specimens are nontoxic to rat MSCs. When cultivated with the test materials or on their surfaces, the MSCs retain the viability, adhesion, morphology, and capability for proliferationin vitro, as evidenced by cell counting in a Goryaev chamber, MTT test, flow cytometry, and light and fluorescence microscopy. The unimplanted NiTi specimens fail to stimulate MSC proliferation, and this allows the assumption of bioinertness of their surface layers. Conversely, the ion-implanted NiTi specimens reveal properties favorable for MSC proliferation on their surface.

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Negative ion implantation for pattering mesenchymal-stem cell adhesion on silicone rubber and differentiation into nerve cells with keeping their adhesion pattern

Surface and Coatings Technology ◽

10.1016/j.surfcoat.2009.02.076 ◽

2009 ◽

Vol 203 (17-18) ◽

pp. 2562-2565 ◽

Cited By ~ 8

Author(s):

Hiroshi Tsuji ◽

Piyanuch Sommani ◽

Mitsutaka Hattori ◽

Tetsuya Yamada ◽

Hiroko Sato ◽

...

Keyword(s):

Stem Cell ◽

Cell Adhesion ◽

Mesenchymal Stem Cell ◽

Ion Implantation ◽

Silicone Rubber ◽

Nerve Cells ◽

Negative Ion ◽

Adhesion Pattern

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Nanograins on Ti-25Nb-3Mo-2Sn-3Zr alloy facilitate fabricating biological surface through dual-ion implantation to concurrently modulate the osteogenic functions of mesenchymal stem cells and kill bacteria

Journal of Material Science and Technology ◽

10.1016/j.jmst.2020.07.048 ◽

2021 ◽

Vol 73 ◽

pp. 31-44

Author(s):

Run Huang ◽

Lei Liu ◽

Bo Li ◽

Liang Qin ◽

Lei Huang ◽

...

Keyword(s):

Stem Cells ◽

Mesenchymal Stem Cells ◽

Ion Implantation ◽

Biological Surface

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Nerve-cell attachment properties of polystyrene and silicone rubber modified by carbon negative-ion implantation

Surface and Coatings Technology ◽

10.1016/j.surfcoat.2006.01.074 ◽

2007 ◽

Vol 201 (19-20) ◽

pp. 8123-8126 ◽

Cited By ~ 13

Author(s):

H. Tsuji ◽

P. Sommani ◽

T. Kitamura ◽

M. Hattori ◽

H. Sato ◽

...

Keyword(s):

Ion Implantation ◽

Nerve Cell ◽

Silicone Rubber ◽

Cell Attachment ◽

Negative Ion

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The Effect of POSS-COOH/Silicone Rubber (SR) on the Adipogenesis Differentiation of Mesenchymal Stem Cells

Journal of Biomaterials and Tissue Engineering ◽

10.1166/jbt.2019.2058 ◽

2019 ◽

Vol 9 (6) ◽

pp. 760-769

Author(s):

Yichen Du ◽

Xiaohua Shi ◽

Xin Zhou ◽

Yao Chen ◽

Hailin Wang ◽

...

Keyword(s):

Stem Cells ◽

Mesenchymal Stem Cells ◽

Soft Tissue ◽

Surface Morphology ◽

Silicone Rubber ◽

Adipogenic Differentiation ◽

Filling Material ◽

Cell Compatibility ◽

Liquid Silicone ◽

Good Biocompatibility

The aim of this study was to prepare an engraving silicone rubber (SR) material with good biocompatibility and functionality. SR with different POSS-COOH proportions was produced by mixing POSS-COOH nanoparticles in medical liquid silicone. The viability, adhesion and differentiation of MSCs on the surface of the modified materials were examined. Compared with virgin SR, the hydrophilic property of the composites was obviously increased and hardness was decreased; however, there was no obvious change in the surface morphology of the POSS-COOH/SR. The results of MTT showed that the viability of MSCs on POSS-COOH/SR was significantly suppressed (P < 0.05). Furthermore, POSS-COOH/SR was able to induce significant adipogenesis differentiation of MSCs. Our results demonstrated POSS-COOH/SR not only improves cell compatibility, it promotes the adipogenic differentiation of MSCs. It may be used as a new soft tissue-filling material in the field of plastic surgery.

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