FLEET Velocimetry for Aerodynamics

Long-lasting emission from femtosecond excitation of nitrogen-based flows shows promise as a useful mechanism for a molecular tagging velocimetry instrument. The technique, known as femtosecond laser electronic excitation tagging (FLEET), was invented at Princeton a decade ago and has quickly been adopted and used in a variety of high-speed ground test flow facilities. The short temporal scales offered by femtosecond amplifiers permit nonresonant multiphoton excitation, dissociation, and weak ionization of a gaseous medium near the beam's focus without the generation of a laser spark observed with nanosecond systems. Gated, intensified imaging of the resulting emission enables the tracking of tagged molecules, thereby measuring one to three components of velocity. Effects of local heating and acoustic disturbances can be mitigated with the selection of a shorter-wavelength excitation source. This review surveys the development of FLEET over the decade since its inception, as it has been implemented in several test facilities to make accurate, precise, and seedless velocimetry measurements for studying complex high-speed flows.

Test for Reliability for Mission Critical Applications

Test for Reliability is a test flow where an Integrated Circuit (IC) device is continuously stressed under several corner conditions that can be dynamically adapted based on the real-time observation of the critical signals of the device during the evolution of the test. We present our approach for a successful Test-for-Reliability flow, going beyond the objectives of the traditional reliability approach, and covering the entire process from design to failure analysis.

Formulation and evaluation of Kirinyuh Leaf effervescent granules (Chromolaena Odorata. L) as an antioxidant

Introduction: Kirinyuh leaf is a widely grown plant in Indonesia, containing alkaloids, flavonoids, saponins, tannins, and steroids. Flavonoids are compounds that can capture free radicals or act as natural antioxidants. Effervescent granules can mask the bitter taste and simplify the dissolving process without involving manual stirring. Objective: The purpose of this study was to make and evaluate a formulation of effervescent granules of Kirinyuh leaf extract. Methods: The granule method was carried out by the wet granulation method. Granule evaluation included organoleptic test, water content test, dissolve time test, flow time test, pH test, and hedonic test. Results: Organoleptic test results showed similar granule size, slightly brownish colour, and characteristics of Kirinyuh leaf odour. When examining their quality, the granules produced met the requirements, with moisture content between 0.4% and 0.7%, dissolving time of 30-35 seconds, flow time test of 8-8.5 g/second, and pH of 5.6-5.8; the results of the hedonic test showed that the effervescent granule preparation was much preferred.

Pt(II)-Thiocarbohydrazone Complex as Cytotoxic Agent and Apoptosis Inducer in Caov-3 and HT-29 Cells through the P53 and Caspase-8 Pathways

In this study, a platinum(II) complex ([Pt(H2L)(PPh3)] complex) containing a thiocarbohydrazone as the ligand was tested as an anti-proliferative agent against ovarian adenocarcinoma (Caov-3) and human colorectal adenocarcinoma (HT-29) through MTT assays. Apoptotic markers were tested by the AO/PI double staining assay and DNA fragmentation test. Flow cytometry was conducted to measure cell cycle distribution, while the p53 and caspase-8 pathways were tested via immunofluorescence assay. Results demonstrated that the cytotoxic effect of the Pt(II)-thiocarbohydrazone complexes against Caov-3 and HT-29 cells was highly significant, and this effect triggered the activation of the p53 and caspase-8 pathways. Besides, apoptosis stimulated by the Pt(II)-thiocarbohydrazone complex was associated with cell cycle arrest at the G0/G1 phase. These findings suggest that the target complex inhibited the proliferation of Caov-3 and HT-29 cells, resulting in the arrest of the cell cycle and induction of apoptosis via the stimulation of the p53 and caspase-8 pathways. The present data suggests that the Pt(II)-thiocarbohydrazone complex could also be a promising chemotherapeutic agent for other types of cancer cells.

VARIASI EKSTRAK ETANOL BIJI KEBIUL (Caesalpinia bonduc (L). Roxb) PADA FORMULASI SEDIAAN EMULSI M/A

Indonesia is an archipelago state that is the nature have provides raw materials from medicinal plants, it is not yet used cause medicinal plants usually lived in the forests that have difficult access. Kebiul seed (Caesalpinia bonduc (L.) Roxb.) is an empirically medicinal plant belonging to the Caesalpiniaceae family which has great potential to be used as an prevent from free radicals or as an antoxidants cause kebiul seeds contains alkaloids, flavonoids, saponins, and steroids.The aims of this research to make a M/A emulsion substance. The sample that have been used in this study is kebiul seeds that have extracated by maceration and then evaporated with a waterbath. The extract was made in four formulas with each concentration 10%, 20%, 30%. The evaluation of the M/A have perfect result which were done, stability test (odor, color and shape / texture and pH test) flow properties test, viscosity test, emulsion type test, and microscope cross-section test. After analyzing the M/A emulsion substance the results from all of the evaluations that have been done from the 4 formulas, the kebiul seeds could be made into M/A Emulsion. Variation in contents of Caesalpinia bonduc (L.) Roxb extract which have the requirements evaluation, namely: organoleptic test, pH test, stability test, emulsion type test, viscosity test, and microscope cross-section test.

Immunopathogenetic features and prognostic criteria for severe hemorrhagic fever with renal syndrome

Aim. Assessment of the features of cellular immunological mechanisms at the early stage of HFRS of varying severity and development of prognostic criteria for the risk of a severe course of the infectious process. Materials and methods. An immunological blood test (flow cytofluorimetry method) was performed in 12 patients with severe HFRS and 53 patients with moderate course in the dynamics of the disease. Statistical data processing was performed based on the SPSS software package. Results. At the initial stages of HFRS, immunological features of the severe course of the disease were established in the form of a higher content of T-helper and regulatory T-cells in the blood and a reduced number of CTL, including their activated pool. Based on these changes, an immunological prognostic coefficient of HFRS was developed, which allows determining the risk of severe course in the early days (febrile period) of the disease with high prognostic accuracy. Conclusion. The results obtained allowed us to identify previously unknown features of the immune process at the initial stages of HFRS development, which allowed us to propose a new approach to predicting the severe course of the disease.

Combinatory Treatment with miR-7-5p and Drug-Loaded Cubosomes Effectively Impairs Cancer Cells

Background: Multidrug resistance (MDR) is an emerging problem in the treatment of cancer. Therefore, there is a necessity for novel strategies that would sensitize tumor cells to the administered chemotherapeutics. One of the innovative approaches in fighting drug-resistant tumors is the treatment of cancer with microRNA (miRNA), or the use of cubosomes (lipid nanoparticles) loaded with drugs. Here, we present a study on a novel approach, which combines both tools. Methods: Cubosomes loaded with miR-7-5p and chemotherapeutics were developed. The effects of drug- and miRNA-loaded vehicles on glioma- (A172, T98G), papillary thyroid- (TPC-1) and cervical carcinoma-derived (HeLa) cells were analyzed using molecular biology techniques, including quantitative real-time PCR, MTS-based cell proliferation test, flow cytometry and spheroids formation assay. Results: The obtained data indicate that miR-7-5p increases the sensitivity of the tested cells to the drug, and that nanoparticles loaded with both miRNA and the drug produce a greater anti-tumor effect in comparison to the free drug treatment. It was found that an increased level of apoptosis in the drug/miRNA co-treated cells is accompanied by an alternation in the expression of the genes encoding for key MDR proteins of the ABC family. Conclusions: Overall, co-administration of miR-7-5p with a chemotherapeutic can be considered a promising strategy, leading to reduced MDR and the induction of apoptosis in cancer cells.