scholarly journals Effect of injection hatching eggs with Newcastle disease vaccine and different doses of vitamin E on some productive traits and immune response of broilers

2015 ◽  
Vol 39 (2) ◽  
pp. 98-107
Author(s):  
Mushtaq T. Abdulwahid

     This study aimed to investigate the effects of injection hatching eggs with different doses of vitamin E on productive traits, some physiological and immune response to Newcastle disease vaccine. Two hundred fertile eggs of broiler breeder Ross (308) strain were incubated in automatic incubator machine after divided into four treatments (50 eggs per treatment) with two replicates. First treatment was injected 0.1ml/egg of phosphate buffer sterile into amniotic fluid at day 18th of incubation which it was considered as control group, second treatment was injected with 0.1ml/egg of inactivated Newcastle disease vaccine, third treatment was injected with 0.1ml/egg of inactivated Newcastle disease vaccine and 0.1 ml/egg of vitamin E, fourth treatment was injected with 0.1ml/egg of inactivated Newcastle disease vaccine and 0.15 ml/egg of vitamin E. All injected eggs were carried back into incubator for complete hatching process. Hatched chicks were transferred to the hall in farm of the Veterinary Medicine College/University of Baghdad; therefore, the chicks were distributed into four treatments with two replicates depending on the previously treated groups until fifth week of age. The results revealed that the treated groups with vitamin E were significantly (P≤0.05) increased in hatchability percentage, body weight, weight gain, as well as significant reduce in feed intake and improvement in feed conversion ratio as compared with second group and control, the results of third group showed significantly increased (P≤0.05) in antibody titers against Newcastle disease virus at aged (21 and 35) day as compared with the other treated groups and control.

2019 ◽  
Vol 37 (2) ◽  
pp. 199-205
Author(s):  
Mushtaq T. Abdulwahid

Four hundred fertile eggs of broiler breeder Ross strain were incubated in commercial Hatchery. Eggs were injected into amniotic fluid on 18th day of incubation after divided into four treatment groups (100 eggs per treatment) with two replicates. First treatment was injected 100 µl of PBS, second treatment was injected with100 µl of inactivated ND vaccine, the third treatment was injected with 100 µl of inactivated ND vaccine and 50 µl vitamin E (oily form) and finally the fourth treatment was injected with 100 µl of inactivated ND vaccine and 50 µl Cod liver oil. All injected eggs were carried back into hatchery for complete hatching process. Hatched chicks were transported to the farm of the Veterinary Medicine College/University of Baghdad for 42 days from 6/10/2011 to 16/11/2011. The hatched chicks from the previously treated groups were distributed into four treatments with two replicates for up to the experimental end. Results of treated groups showed significant (P ≤ 0.05) increase in body weight, weight gain, as well as significant reduce in feed intake and improvement in feed conversion ratio, also, antibody titers against Newcastle disease virus of treated groups showed significant (P ≤ 0.05) increase progressively with age until the end of the experiment compared with broiler chicks of control.


2016 ◽  
Vol 47 (3) ◽  
Author(s):  
ABDULWAHID & et al.

This study was aimed to enhance the immune response of the broilers by vitamin E and Cod liver oil supplement with locally inactivated bivalent vaccine of Newcastle disease and Infectious bronchitis viruses. One hundred and twenty broiler chicks Ross 308 were divided to four groups at 30 chicks of each group. The 2nd group was vaccinated with 0.5ml/chick of locally inactivated ND-IB vaccine at 5 days old. The 3rd group was vaccinated with 0.5ml locally ND-IB vaccine and 0.1 ml/chick of VE. The 4th group was vaccinated with 0.5ml of locally ND-IB vaccine and 0.1ml/chick of Cod liver oil, while the first group was left without vaccination as a control group. All groups were vaccinated with attenuated ND-IB at 3 days old and attenuated IBD at 14 days old. The results of ELISA test revealed that the 3rd and 4th groups showed significantly increased (P≤0.05) in Ab titers against ND virus at aged 35 days, also, the 4th group showed significantly (P≤0.05) increased in Ab titers against IB virus at day 35 of age as compared with the other groups. The 4th group showed a significant (P≤0.05) increased in bursal index as compared with the other groups. The results of challenge test revealed that vaccinated groups resist the velogenic ND and IB viruses with relatively high productive range particularly the 4th group as compared with other groups. In conclusion, the VE and Cod liver oil supplement with local bivalent oil based vaccine of ND and IBV may enhance the immune response of the broilers particularly the 4th group which vaccinated with 0.5 ml of local bivalent oil based vaccine of ND-IB vaccine and 0.1 ml Cod liver oil.


2004 ◽  
Vol 45 (1) ◽  
pp. 55-66
Author(s):  
A. M.H. AL-Judi ◽  
R. H. Hameed ◽  
A. D. Salman

This study was done on 57 Awassi pregnant ewes, divided in to 5 groups, the 1st control group, the second group received  E (900 mg) and Selenium (3mg), twicel.M.injections of vitamin the third group received one injection of vitamin E (900mg) and  feed  via selenium ( 3 mg), the fourth group received vitamin E block (1 gm) daily for two weeks, the fifth group received  vitamin E via feed block for one week (1gm daily), and all groups injected with C Baghdad vaccine.  This study had been shown a significant difference in antibody titer between treated groups and the control group, from2weeks post  titre vaccination There were significant difference in antibody (p<0.05) between lambs of treated and control group. While there were no significant difference in body weight of ewes and lambs,  production of treated and but was a significant difference in milk control group. 


Gene ◽  
2021 ◽  
Vol 766 ◽  
pp. 145077
Author(s):  
Liang-xing Guo ◽  
Fu-rong Nie ◽  
An-qun Huang ◽  
Rui-ning Wang ◽  
Meng-yun Li ◽  
...  

2021 ◽  
Vol 44 (3) ◽  
pp. 110-116
Author(s):  
E. B. Oluwayinka ◽  
F. S. Okunuga ◽  
O. O. Oni ◽  
J. O. Olukunle ◽  
O. A. Akinkuotu ◽  
...  

Poultry production worldwide is still challenged by diseases and researchers are continually searching for different means of combating the causative agents. Annona muricata's antiherpes and anticancer activities may be helpful in tackling Mareks disease of poultry birds. This study was carried out to investigate the effects of aqueous extract of Annona muricata leaves on the haematology, some liver enzymes, body weight gain and immune response to Newcastle disease vaccination in broilers at dosages of 50mg/kg, 100mg/kg and 200mg/kg. Twenty (20) Marshall Broilers which were 35days old at the start of the experiment were used; the chicks were divided into 4 groups. The aqueous extract of Annona muricata leaves was administered orally for 14days; Blood and serum samples were collected at the beginning and end of experiment. Haematology, some liver enzymes test and haemagglutinition inhibition (HI) test against Newcastle disease vaccination was carried out. A significant (p<0.05) decreased in antibody titre against Newcastle disease vaccine was observed in only the group of birds fed with 200mg/kg of the extract. Birds fed with 200mg/kg of the extract had the highest values of packed cell volume (PCV), haemoglobin (Hb), red blood cell (RBC) and white blood cell (WBC) count but there was no significant difference (p<0.05) in these values in comparison to the control group. There was no significant difference (p<0.05) in the levels of aspartate aminotransferase (AST), alanine aminotransferase (ALT) and alkaline phosphatase (ALP) between treated and control groups. Therefore, aqueous extract of Annona muricata reduces immune response to Newcastle disease vaccine at 200mg/kg dosage but does not have any deleterious effect against the haematology and liver functions of birds fed with doses as high as 200mg/kg.


2014 ◽  
Vol 3 (1) ◽  
pp. 150-157
Author(s):  
Khalid M. Gaafar

The research was conducted to study the effect of feeding broiler chickens on diets containing isomaltooligosaccharides on the growth performance, carcass traits and immune response. 90-one day old broiler chicks were used according to completely randomized two treatment groups and one control, 30 birds each. Birds fed ad-libitum on basal starter and grower-finisher diets for 35 day. Diets of treatment`s groups contained 0.5 g/Kg and 1 g/Kg of Isomaltooligosaccharides, while the control group fed on the basal diets without Isomaltooligosaccharides supplementation. Dietary supplementation of broiler chickens with Isomaltooligosaccharides improved body weight, feed conversion, carcass traits, two lymphoid organs weight and log antibody titer against avian flu vaccine. Most of the highest values were for birds fed low levels of Isomaltooligosaccharides. Feed intake decreases as Isomaltooligosaccharides level increases. Dietary supplementation with Isomaltooligosaccharides did not affect the lipids profile (triglycerides, total cholesterol, LDL and HDL), however the blood VLDL levels decreased with increased levels of Malondialdehyde and Glutathione reductase. Collectively, Dietary supplementation of broiler chickens with 0.5 g/Kg diet of Isomaltooligosaccharides improved growth performance, carcass traits and immune status.


2013 ◽  
Vol 16 (4) ◽  
pp. 619-627 ◽  
Author(s):  
M. Pogány Simonová ◽  
A. Lauková ◽  
L. Chrastinová ◽  
I. Plachá ◽  
V. Strompfová ◽  
...  

Abstract The effect of Enterococcus faecium CCM7420 (EF) - enterocin-producing and probiotic strain of rabbit origin, Eleutherococcus senticosus extract (ES) and their combination (ES+EF) was determined on selected bacteria in faeces and caecum content, leukocytes phagocytosis, blood biochemistry and growth performance. Ninety-six weaned rabbits were divided into 3 experimental (ES, EF, ES+EF) and control group (CG). The rabbits in the groups ES and EF+ES were fed commercial diet enriched with E. senticosus extract (30 g/100 kg feed), rabbits in groups EF and CG were fed untreated diet. The rabbits in the EF and ES+EF groups were administered with an overnight culture of E. faecium CCM7420 strain (500 μl/animal/day into water, 109 CFU/ml). The treatment period lasted 21 days. The microbiological examinations in faecal samples confirmed the presence of E. faecium CCM7420 strain. In groups EF and ES+EF, the reduction of faecal coliforms, Pseudomonas-like sp., Clostridium-like sp. and S. aureus was recorded. Leucocyte phagocytosis significantly increased in all experimental groups (P<0.0001) compared to CG. The lowest GPx values were measured in the ES+EF group. Higher total protein, triglycerides and calcium concentrations were detected in experimental groups compared to CG. The cholesterol concentration decreased in the ES group. The highest average daily gain was recorded in EF group; in ES+EF the better feed conversion ratio and no mortality was recorded. These results indicated that the dietary supplementation with the E. faecium CCM7420 and E. senticosus extract stimulate the leukocytes phagocytosis and reduces the potential pathogens in rabbits digestive tract without oxidative stress and improve the growth performance.


The aim of the study was to investigate the effects of Moringa oleifera aqueous seed extract on live body weight, gonadal and extragonadal dimensions and sperm reserves of Yankasa rams. Twenty five apparently healthy Yankasa rams aged 1-2 years and weighing 19.0 ± 2.1 Kg were used for the study. The rams were randomly selected into five groups: A, B, C, D and E with five rams in each group as treatment and control groups respectively. Groups A - D were given oral dose of Moringa oleifera aqueous seed extract at a dose rate of 1000, 2000, 3000, 4000 (mg/kg), respectively while group E was given 10 ml/kg water orally, daily for five months. Live body weight, gonadal and extragonadal reserves were determined according to standard techniques. The results showed a significant increase in live body weight in the months of April to June among rams treated with different doses of Moringa oleifera aqueous seed extract compared with the control group. The control group showed no significant differences in the body weight, gonadal and extragonadal dimensions and sperm reserves. In conclusion, the treatment of Yankasa rams with Moringa oleifera aqueous seed extract increased live body weight, but had no significant effects on gonadal and extragonadal dimensions and sperm reserves in Yankasa rams. Therefore, it is recommended that M. oleifera aqueous seed extract can be used at doses of 2000mg/kg to 3000mg/kg in Yankasa rams for optimum gain in live body weight.


2016 ◽  
Vol 37 (4) ◽  
pp. 1947 ◽  
Author(s):  
Kelly Mazutti ◽  
Leandro Batista Costa ◽  
Lígia Valéria Nascimento ◽  
Tobias Fernandes Filho ◽  
Breno Castello Branco Beirão ◽  
...  

For the last several decades, antimicrobial compounds have been used as feed additives to promote piglet growth at weaning, through the prevention of subclinical and clinical disease. However, few studies have assessed the influence of these antibiotics on the immune response of nursery pigs, as well as the relation between performance, health, and immunity of animals that receive feed additives. Therefore, the present study aimed to evaluate the effects of colistin and tylosin when used as feed additives on the performance, incidence of diarrhea, and immune response of nursery pigs. In this study, 72 weaned pigs (average age, 28 days) were allotted into one of three treatment groups: a control group (feed with no antibiotics), tylosin group (feed containing 22 ppm tylosin), and colistin group (feed containing 20 ppm colistin). Weekly, during a five week period, the average daily feed intake, average daily gain, and feed conversion ratio of the pigs were evaluated. Stools were scored daily, in accordance with a fecal texture scale. Blood samples were collected on the day of housing (d0) and on d7, d21, d28, and d35 for immune cell phenotyping. The results of this study showed that piglets in both the colistin and tylosin groups exhibited a significantly higher average daily feed intake, resulting in a higher body weight at the end of the experimental period (d35) when compared with piglets from the control group. Colistin and tylosin also significantly reduced the incidence of diarrhea. Colistin and tylosin modulated the piglets’ immune responses, particularly on d28, by changing the percentage of circulating B lymphocytes, CD4+CD8+ T cells, and the CD4:CD8 ratio.


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