scholarly journals Study of flavonoids and phenolic acids in green tea leaves

2021 ◽  
Vol 14 (3) ◽  
pp. 287-291
Author(s):  
O. Yu. Maslov ◽  
S. V. Kolisnyk ◽  
M. A. Komisarenko ◽  
E. Yu. Akhmedov ◽  
S. M. Poluian ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Green Tea ◽  
Phenolic Acids ◽  
High Performance ◽  
Flavonoid Glycosides ◽  
Qualitative Composition ◽  
Tea Leaves ◽  
Quantitative Content ◽  
Green Tea Leaves

The aim of work is study qualitative composition and quantitative content of flavonoids and phenolic acids in green tea leaves. Material and methods. The object of the study was green tea leaves, which were collected in Anhui Province, China. The analysis of 60  % ethanolic extract from green tea leaves was performed by high-performance liquid chromatography using a Prominence LC-20 Shimadzu chromatographic system (Japan) with an SPD-20AV spectrophotometric detector, an Agilent Technologies Microsorb-MV-150 column (reversed-phase, C18 modified silica gel, length – 250 mm, diameter – 4.6 mm, particles size – 5 μm). Identification of substances in the extract was carried out by comparing the retention time and the spectral characteristics of the test substances with the same characteristics of the reference standards. Results. 13 compounds were identified and determined by high-performance liquid chromatography. Among flavonoid aglycones quantitatively dominated by quercetin (0.35 %), in the case of flavonoid glycosides, it was luteolin-6-C-glycoside (1.30 %) and among phenolic acids, it was gallic acid (5.21 %). Conclusions. The qualitative composition, quantitative content of flavonoids and phenolic acids in the green tea leaves were determined by high-performance liquid chromatography. According to HPLC, the content of flavonoids in green tea leaves was higher than the content of phenolic acids.

scholarly journals Determination of catechins in green tea leaves by HPLC compared to spectrophotometry

2021 ◽  
Vol 19 (3(75)) ◽  
pp. 28-33
Author(s):  
Oleksandr Yu. Maslov ◽  
Mykola A. Komisarenko ◽  
Yulia S. Kolisnyk ◽  
Tatyana A. Kostina
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Green Tea ◽  
High Performance ◽  
Raw Material ◽  
Qualitative Composition ◽  
Tea Leaves ◽  
Quantitative Content ◽  
Significant Difference ◽  
Green Tea Leaves

Aim. To study the qualitative composition, the quantitative content of catechins in green tea leaves and compare the data obtained with those evaluated by spectrophotometry.Materials and methods. Green tea leaves used for the analysis were collected in Anhui Province, China. The extract for the HPLC analysis was obtained by the maceration method with 60 % ethanol twice in the raw material/extractant ratio of 1 : 20. In the case of the spectrophotometric analysis, green tea leaves were extracted with 70 % ethanol twice by the maceration method in the raw material/extractant ratio of 1 : 20. The analysis of the extract from green tea leaves was performed by high performance liquid chromatography using a Prominence LC-20 Shimadzu chromatographic system (Japan) with a SPD-20AV spectrophotometric detector, an Agilent Technologies Microsorb-MV-150 column (reversed phase, C18 modified silica gel, length – 150 mm, diameter – 4.6 mm, particles size – 5 μm). Substances in the extract were identified by comparing the retention time and the spectral characteristics of the test substances with the same characteristics of the reference standards. Spectrophotometric measurements were carried out using a UV-1000 single beam spectrophotometer (China) with the pair of S90-309Q quartz square cells.Results and discussion. Using high performance liquid chromatography 5 catechins were identified. Among them epigallocatechin-3-O-gallate (10.85 %) predominated, while catechin (0.61 %) had the lowest concentration. The total amount of catechins in green tea leaves was 30.56 and 24.79 % by HPLC and spectrophotometry, respectively. The F- and t-tests showed that there was no significant difference between the results of HPLC and spectrophotometry.Conclusions. The qualitative composition and the quantitative content of catechins have been determined in the extract from green tea leaves by high performance liquid chromatography and spectrophotometry. Both HPLC and spectrophotometric methods can be used to determine the total catechin content in green tea leaves. The high content of catechins makes the extract promising for further study and creation of new herbal medicinal products and dietary supplements. The results obtained will be used for standardization of green tea leaves and for future pharmacological research of its extract.

scholarly journals The study of polyphenolic compounds of Pimpinella anisum herb and determination of their antioxidant activity

2021 ◽  
Vol 19 (1(73)) ◽  
pp. 42-47
Author(s):  
U. Umarov ◽  
S. V. Kolisnyk ◽  
O. V. Kolisnyk ◽  
M. Fatkhullaeva ◽  
N. K. Chinibekova ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Ethanolic Extract ◽  
Polyphenolic Compounds ◽  
Qualitative Composition ◽  
Pimpinella Anisum ◽  
Quantitative Content

Aim. To study the qualitative composition and quantitative content of the polyphenolic compounds in Pimpinella anisum herb and determine their antioxidant activity.Results and discussion. Among the polyphenolic compounds contained in anise herb, chlorogenic acid(4.409 mg/g) predominates. Signifcant amounts of catechins (3.104 mg/g), apigenin derivatives (3.077 mg/g) and luteolin (1.864 mg/g) also accumulate in the herb. Minor amounts of myricetin (0.105 mg/g) and naringenin (0.019 mg/g) derivatives, rutin (0.189 mg/g), quercetin (0.028 mg/g), apigenin (0.009 mg/g) and hesperetin (0.002 mg/g) are present. According to the research results, the antioxidant activity of polyphenolic compounds of anise herb with reference to ascorbic acid was found to be 67.76 ± 0.05 mmol/g. Rutin exhibited the antioxidant activity at the level of 3979.59 ± 0.08 mmol/g.Experimental part. Pimpinella anisum herb collected during the flowering stage in the summer of 2019 inthe Kharkiv region (Ukraine) was used for analysis. The analysis of 70 % ethanolic extract from anise herb was performed by high performance liquid chromatography using a Prominence LC-20 Shimadzu chromatographic system (Japan) with a SPD-20AV spectrophotometric detector, an Agilent Technologies Microsorb-MV-150 column (reversedphase, C18 modifed silica gel, length – 150 mm, diameter – 4.6 mm, particles size – 5 μm). Identifcation of substances in the extract was carried out by comparing the retention time and the spectral characteristics of the test substances with the same characteristics of the reference standards. The antioxidant activity was determined by the potentiometric method (pH meter – Hanna 2550, with redox electrode EZDO PO50) with reference to ascorbic acid.Conclusions. The qualitative composition and quantitative content of polyphenolic compounds in the ethanolic extract of anise herb have been determined by high performance liquid chromatography. The total content of polyphenolic compounds is 17.576 mg/g. The antioxidant activity of polyphenolic compounds of anise herb with reference to ascorbic acid has been found to be 67.76 ± 0.05 mmol/g.Key words: polyphenolic compounds; anise; herb; high performance liquid chromatography; antioxidant activity

scholarly journals The investigation of the amino acids content and polysaccharides in the above and underground parts of Primula veris L.

2015 ◽  
Vol 17 (4) ◽  
Author(s):  
L. H. Shostak ◽  
S. M. Marchyshyn ◽  
M. I. Lukanyuk ◽  
O. L. Demydyak
Keyword(s):  
Amino Acids ◽  
Gas Chromatography ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Free Amino ◽  
High Performance ◽  
Qualitative Composition ◽  
Plant Materials ◽  
Quantitative Content ◽  
Primula Veris

<p>There were determined the amino acids contents and complex of polysaccharides of the above and underground parts of Primula veris L. In the plant materials it was established the general content of amino acids and the content of free amino acids by high performance liquid chromatography. Ther were identified from 21 to 23 amino acids and 7 were essential. It was determined their quantity content. The fractions of soluble polysaccharides and pectins were isolated from the studied materials and determined their contents. In the leaves, flowers and rhizomes with<br />roots there were established the qualitative composition and quantitative content of the free saccharides by the gas chromatography.</p>

Content of amino acids in raw materials of somespecies of ASTERACEAE family

Fitoterapia ◽  
2020 ◽  
Vol 3 (3) ◽  
pp. 44-50
Author(s):  
S.M. Marchyshyn ◽  
◽  
L.V. Slobodianiuk ◽  
R.Yu. Basaraba ◽  
N.А. Hudz ◽  
...  
Keyword(s):  
Amino Acids ◽  
High Performance Liquid Chromatography ◽  
Free Amino ◽  
High Performance ◽  
Raw Materials ◽  
Essential Amino Acids ◽  
Qualitative Composition ◽  
Quantitative Content ◽  
First Time ◽  
Asteraceae Family

Keywords: amino acids; common pussytoes; yacon; stevia; golden marigolds; leaves; herb; aster family; high performance liquid chromatography. For the first time the qualitative composition was studied and the quantitative content of amino acids in the herb of common pussytoes, yacon leaves, stevia leaves, herb of golden marigolds was determined by HPLC. 17 bound and 16 free amino acids were identified in the herb of common pussytoes, in the herb of golden marigolds, yacon leaves and stevia leaves – 12 and 5, 17 and 11 and 16 and 14 amino acids, respectively. The highest content of the sum of essential and substitute amino acids is contained in the leaves of stevia; the lowest content of the sum of essential amino – in the herb of golden marigolds, the sum of essential amino acids – in the herb of common pussytoes.

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