scholarly journals Effects of vitamins E and Riboflavin (B2) and combinations of them on the hematological parameters of common carp, Cyprinus carpio L., fingerlings

2015 ◽  
Vol 23 (2) ◽  
pp. 107-111 ◽  
Author(s):  
Seyedeh Atefeh Sharifzadeh ◽  
Hossein Khara ◽  
Shayan Ghobadi
Keyword(s):  
Vitamin E ◽  
Common Carp ◽  
Blood Cells ◽  
Hematological Parameters ◽  
The Other ◽  
Vitamin B ◽  
Mean Corpuscular Hemoglobin ◽  
Corpuscular Hemoglobin ◽  
Common Carp Cyprinus Carpio ◽  
Experimental Treatments

Abstract In the present study, the effects of vitamins E, B2 and combinations of them on hematological parameters of common carp, Cyprinus carpio L., were investigated during a 56-day experiment. The various dietary levels of vitamins E, B2 and their combination were used as experimental treatments, as follows: T1: vitamin E (80 mg), T2: vitamin E (160 mg kg diet-1), T3: vitamin E (240 mg kg diet-1), T4: vitamin B2 (7 mg kg diet-1), T5: vitamin B2 (15 mg kg diet-1), T6: vitamin B2 (20 mg kg diet-1), T7: vitamin E (80 mg kg diet-1) + vitamin B2 (7 mg kg diet-1), T8: vitamin E (160 mg kg diet-1) + vitamin B2 (15 mg kg diet-1), T9: vitamin E (240 mg kg diet-1) + vitamin B2 (20 mg kg diet-1). One group not given vitamin supplements was the control. The values of red blood cells (RBC), hemoglobin (Hb), and hematocrit (Hct) were higher in T7 than in the other experimental treatments. The values of white blood cells (WBC) were higher in T2 than in the other treatments. The concentration of immunoglobulin (IgM) was also lower in T10 than in the other experimental treatments. The highest values of mean corpuscular hemoglobin (MCH) and mean corpuscular volume (MCV) were observed in T2. No significant differences were noted among experimental groups in the values of mean corpuscular hemoglobin concentration (MCHC). This study showed that vitamin E and B2 supplements alter the hematological parameters of common carp.

scholarly journals Changes in hematological parameters and gill morphology in common carp exposed to sub-lethal concentrations of Malathion

2016 ◽  
Vol 2 (3) ◽  
pp. 370-378 ◽  
Author(s):  
Sadia Sharmin ◽  
Md Abdus Salam ◽  
Farhana Haque ◽  
Md Sadiqul Islam ◽  
Md Shahjahan
Keyword(s):  
Common Carp ◽  
Blood Cells ◽  
Morphological Changes ◽  
Hematological Parameters ◽  
White Blood Cells ◽  
Hemoglobin Concentration ◽  
Blood Parameters ◽  
Mean Corpuscular Hemoglobin ◽  
Agricultural Field ◽  
Corpuscular Hemoglobin

Malathion is an organophosphorous insecticide widely used in the agricultural field in Bangladesh and reach in the aquatic environment through rain wash. In the present study, we examined the effects of Malathion on hematological parameters and gills morphology in common carp exposed to two sub-lethal concentrations (1.5 and 3.0 mg/L) for a period of 192h (8 days). For hematological parameters fish were sacrificed at 24, 48, 72, and 96h after start of exposure. Gills were collected at 192h after start of exposure. The blood glucose level was significantly elevated with increasing the concentration of Malathion. Red blood cells (RBCs), hemoglobin (Hb) and hematocrit (Hct) values were significantly decreased after the exposure of Malathion. Significant increments were observed in case of the mean corpuscular volume (MCV) and mean corpuscular hemoglobin concentration (MCHC), while no distinct changes were noted for mean corpuscular hemoglobin (MCH). Interestingly, white blood cells (WBCs) count was significantly increased with toxicity of Malathion. Several morphological changes, such as telangiectasia, blood lamellar congestion, hypertrophy of filaments, lamellar fusion were observed in the gills of fish exposed to Malathion. The present study revealed that the insecticide had adverse effects on various blood parameters and gills morphology in common carp. Thus, the use of insecticide in the agriculture field may be a threat to fauna and flora of the aquatic environment.Asian J. Med. Biol. Res. September 2016, 2(3): 370-378

scholarly journals Hematology of Wild Caught Dubois’s Tree FrogPolypedates teraiensis, Dubois, 1986 (Anura: Rhacophoridae)

2014 ◽  
Vol 2014 ◽  
pp. 1-7 ◽  
Author(s):  
Madhusmita Das ◽  
Pravati Kumari Mahapatra
Keyword(s):  
Blood Cell ◽  
Blood Serum ◽  
Blood Cells ◽  
Hematological Parameters ◽  
Hemoglobin Concentration ◽  
Mean Corpuscular Hemoglobin ◽  
Reference Ranges ◽  
Corpuscular Hemoglobin ◽  
Differential Leukocyte Count ◽  
Rbc Count

Blood was analyzed from eighty (forty males and forty females) adult individuals ofPolypedates teraiensisto establish reference ranges for its hematological and serum biochemical parameters. The peripheral blood cells were differentiated as erythrocytes, lymphocytes, eosinophils, neutrophils, monocytes, basophils, and thrombocytes, with similar morphology to other anurans. Morphology of blood cells did not vary according to sex. The hematological investigations included morphology and morphometry of erythrocytes, morphometry of leucocytes, packed cell volume (PCV), hemoglobin content (Hb), mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC), erythrocyte or red blood cell (RBC) count, leukocyte or white blood cell (WBC) count, differential leukocyte count, and neutrophil to lymphocyte (N/L) ratio. Besides, protein, cholesterol, glucose, urea, uric acid, and creatinine content of blood serum were assayed. Hematological parameters that differed significantly between sexes were RBC count, length and breadth of RBC, neutrophil %, N/L ratio, area occupied by basophils, and diameter of large lymphocyte and eosinophils. The level of glucose, urea, and creatinine in blood serum also significantly differed between sexes.

scholarly journals Hematological parameters of Iranian cichlid Iranocichla hormuzensis: Coad, 1982 (Perciformes) in Mehran River

2012 ◽  
Vol 84 (4) ◽  
pp. 943-949 ◽  
Author(s):  
Ehsan Daneshvar ◽  
Mahsa Y. Ardestani ◽  
Salar Dorafshan ◽  
Mauricio L. Martins
Keyword(s):  
Blood Cells ◽  
Mean Corpuscular Volume ◽  
High Efficiency ◽  
Hematological Parameters ◽  
White Blood Cells ◽  
Hemoglobin Concentration ◽  
Mean Corpuscular Hemoglobin ◽  
Mean Corpuscular Hemoglobin Concentration ◽  
Corpuscular Hemoglobin ◽  
Food Fish

This study describes the hematological parameters in Iranocichla hormuzensis, an Iranian freshwater cichlid important as ornamental and food fish. Forty fish were captured with seine net at Mehran river Hormozgan province, Iran. Blood was used to determine the total counts of red blood cells (RBC) and white blood cells (WBC), hematocrit, mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC) and morphometric data of erythrocytes. The Iranian fish showed lower RBC and WBC values than the other cichlids (Oreochromis niloticus, O. aureus, O. mossambicus, O. hybrid, Cichlasoma dimerus and Cichla monoculus). Hematocrit did not vary among the species, but MCV, MCH and MCHC in I. hormuzensis were higher than those for O. niloticus, O. aureus, O. hybrid, C. dimerus and C. monoculus. These differences may be related to different life habit of fish. This study suggests that I. hormuzensis is well acclimated to the environment being the first report for its hematology. It is also suggested high efficiency in oxygen transportation, and an efficient inflow of oxygen by the gills, indicating the welfare of fish on this environment.

scholarly journals Effect of ketorolac administration post bone cavitation surgery on some hematological parameters

2017 ◽  
Vol 41 (1) ◽  
pp. 169-177
Author(s):  
Raffal A. Omar
Keyword(s):  
Blood Cells ◽  
Hematological Parameters ◽  
White Blood Cells ◽  
Hemoglobin Concentration ◽  
Treated Group ◽  
Control Group ◽  
Mean Corpuscular Hemoglobin ◽  
Mean Corpuscular Hemoglobin Concentration ◽  
Corpuscular Hemoglobin ◽  
Post Operation

     This study was designed to determine the effect of ketorolac on some hematological parameters post cavitation surgery in domestic rabbits. Ninety-six adult domestic local breed male rabbits were used in this study, weighing (1.23 ± 0.33) kg. They were divided randomly into two groups in the first one (n= 48/group) which represented control group, while the second one (n=48/group) represented the treated group (Ketorolac group). All rabbits in both groups undergoes surgical operation (bone cavitation in femur bone) under general anesthesia. In the Ketorolac group, animals were treated by using 30 mg/kg of Ketorolac directly post-operation and continued daily for 5 days, while in Control group no treatment was given postoperatively. The hematological parameters were recorded which include white blood cells count; red blood cells count; hemoglobin; packed cell volume; mean corpuscular hemoglobin and mean corpuscular hemoglobin concentration; mean corpuscular volume and platelets at the period of (3, 5, 7, 14, 21, 28, 35, and 42) days post operation were estimated. Results concerning hematological values showed no significant differences in packed cell volume values between control and treated group and within each group, while haemoglobin values showed a significant decrease at period 7 and 14 days between control and treated group. Within control group the significant decrease were noticed clearly at period of 7 days. Data concerning mean corpuscular hemoglobin values within control group showed significant decrease were noticed at 5 day, but within treated group, there were no significant differences. mean corpuscular hemoglobin concentration values showed significant decrease ate period 3; 5 and 21 days between control group and treated group respectively. Within control group the significant decrease were noticed obviously at 5 days while within treated group the significant decrease were noticed at 35 days. mean corpuscular volume values showed significant increase at 3 and 5 days period between control and treated groups. Platelets counts within control group showed significant increase at period of 14 days, within treated group the significant increase were recorded at 42 days. Red blood cells showed significant decrease between control and treated groups, while within control group, the significant decrease were at 7 days. White blood cells count within treated group showed significant increase recorded at 21 days. Differential White blood cells count concerning Lymphocytes cells within control group the significant increase were recorded at 42 days  while the significant decrease were noticed at 21 days, but within treated group the significant increase were recorded at 35 days  and significant decrease were noticed at 14 days. Neutrophils counts showed significant decrease at periods 3, 14, 28 and 42 days between control and treated groups respectively; while within control group there were significant decrease at 3 days, but within treated group the significant decrease were noticed at 42 days. Monocytes cells showed significant increase at 35 days between control and treated groups, within control group there were significant increase at 35 days, while within treated group the significant increase recorded at 7 days.

scholarly journals Evaluation of Toxic Effect of Phaleria macrocarpa (Scheff.) Boerl Leaf Extract on Hematological Parameters

1970 ◽  
Vol 8 (3) ◽  
pp. 01-04
Author(s):  
Hanif M Q ◽  
Yuandani Y ◽  
Harahap U
Keyword(s):  
Toxic Effect ◽  
Blood Cells ◽  
Leaf Extract ◽  
Hematological Parameters ◽  
White Blood Cells ◽  
Ethanol Extract ◽  
Mean Corpuscular Hemoglobin ◽  
Corpuscular Hemoglobin ◽  
Phaleria Macrocarpa ◽  
Hematology Parameters

Objectives: This current study was conducted to evaluate the toxic effect of P. macrocarpa leaf extract (Phaleria macrocarpa (Scheff.) Boerl) on hematology parameters in rats. Design: The toxicity of ethanol extract of P. macrocarpa leaf was evaluated by OECD guidelines. The extract at doses of 100, 500, 1000 mg/kg body weight (bw), control satellite and satellite group dose 1000 mg/kg bw were orally administered to the test animal for 90 days. Hematological parameters were observed for 90 days and 118 days for treatment and satellite group, respectively. Interventions: The variable that was intervened in this study was the doses of P. macrocarpa extract. Main outcome measure: The main results in this study were the toxic effect of P. macrocarpa leaf on hematology parameters. Results: The etanol extract of P. macrocarpa did not cause any changes in hematological parameters, these include red blood cells (RBC), hemoglobin, hematocrit, mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC), platelet, white blood cells (WBC), neutrophils, lymphocytes, monocytes, eosinophils, and basophils levels as compared to normal control (P>0.05) Conclusion: The ethanol extract of P. macrocarpa leaf did not cause any toxic effect on hematological parameters for long-term use.  

Effect of gender on hematological parameters in growing Ouled Djellal sheep of semi arid zones of Northeastern Algeria

2021 ◽  
Vol 11 (4) ◽  
pp. 407-417
Author(s):  
Zerari Aicha ◽  
Mahdi Djahida ◽  
Allaoua Sofia Amel ◽  
Rouar Salim
Keyword(s):  
Blood Cells ◽  
Hematological Parameters ◽  
White Blood Cells ◽  
Hemoglobin Concentration ◽  
Mean Corpuscular Hemoglobin ◽  
Arid Zones ◽  
Mean Corpuscular Hemoglobin Concentration ◽  
Corpuscular Hemoglobin ◽  
Mean Values ◽  
Ewe Lambs

Ouled Djellal sheep is the most dominant breed in Algeria due to its adapta-tion to harsh steppe environment. Hematological parameters are good indi-cators of animal health, but their values are breed-specific, and they are affected by age and gender. Therefore, this study aimed to determine the hematological profile of healthy Ouled Djellal lambs and ewe lambs reared traditionally under semi-arid zones of Algeria during the growing peri-od. Blood samples were taken from twenty-four Ouled Djellal lambs (12 males, 12 females) at 42, 63, 84 and 105 days of age to evaluate the count of White Blood Cells, Lymphocyte, Monocyte, Granulocyte, Red Blood Cell and total Platelet, the percentage of Lymphocyte, Monocyte and Granulocyte, Hemoglobin concentration, Hematocrit, Mean Corpuscular Volume, Mean Corpuscular Hemoglobin and Mean Corpuscular Hemoglobin Concentration by usual known methods. Both male and female lambs did not show signifi-cant variations in mean values of White Blood Cells, Lymphocyte count, Red Blood Cells, Hemoglobin, Mean Corpuscular Hemoglobin concentration, per-centage of Monocyte, Monocyte count, Gran percentage and Granulocyte count from 42ͭ ͪ day to 105ͭ ͪ days. Mean values of percentage of Lymphocyte, Hematocrite in lambs, Mean Corpuscular Hemoglobin in ewe lambs, and Mean Corpuscular Volume, Platelets levels in both lambs and ewe lambs indicated also variations during this period of growth. Significant Pearson's correlations were found between some hematological parameters in Ouled Djellal lambs and/or ewe lambs. Therefore, these findings provide usual val-ues, which can be considered for assessing the physiological, nutritional and health status of these animals.

scholarly journals Investigation of hematological parameters related to the severity of COVID-19 disease in Mashhad, Iran

2020 ◽  
Vol 7 (2) ◽  
pp. e19-e19
Author(s):  
Mohsen Seddigh-Shamsi ◽  
Shima Nabavi Mahali ◽  
Mahnaz Mozdourian ◽  
Abolghasem Allahyari ◽  
Neda Saeedian ◽  
...  
Keyword(s):  
Blood Cells ◽  
Hematological Parameters ◽  
White Blood Cells ◽  
Polymerase Chain Reaction Test ◽  
Healthcare Personnel ◽  
Mean Corpuscular Hemoglobin ◽  
Corpuscular Hemoglobin ◽  
Distribution Width ◽  
Volume Number ◽  
Time Of Admission

Introduction:Human coronavirus disease 2019 (COVID-19) is an emerging respiratory disease that has become pandemic worldwide. The patients have a range of mild to severe symptoms, such as pneumonia, acute respiratory distress syndrome, arrhythmia, shock, and other organs involvement. Clinical and hematological evidence can serve as a prognostic factor to detect severe cases of COVID-19. Objectives: The present study aimed to evaluate hematological parameters related to the severity of the disease in COVID-19 patients in Mashhad, Eastern Iran. Patients and Methods: All the individuals with positive real-time reverse transcription-polymerase chain reaction test results and the patients with clinical symptoms whose computed tomography scan results were matched with the diagnosis of viral pneumonia were included in the study. The hematological examinations and complete blood count of the patients were performed by the healthcare personnel through collecting blood samples at the time of admission and discharge from the hospital. Results: In total, 189 COVID-19 patients were included in the study, 47.6% and 58.73% of whom were over 60 years old and male, respectively. Investigation of the hematological parameters revealed that the number of white blood cells, mean value of corpuscular volume, number of platelets, and lymph count had significantly increased at the time of discharge (P<0.01). On the other hand, red blood cells, hemoglobin, hematocrit, mean corpuscular hemoglobin, mean corpuscular hemoglobin, and red cell distribution width values were significantly higher at the time of admission in comparison to discharge (P<0.01). Furthermore, it was found that male gender had a significant relationship with the development of lymphopenia in the patients (P=0.021). Conclusion: It can be concluded that hematological parameters and inflammatory biomarkers in COVID-19 patients could potentially act as the independent factors and affect the prognosis of the disease.

scholarly journals Evaluation of Acute Toxicity of Ethanol Extract of Pirdot Leaf (Saurauia vulcani Korth.) in Rats

2020 ◽  
Vol 3 (2) ◽  
pp. 13-18
Author(s):  
Nerly Juli Pranita Simanjuntak ◽  
Rosidah ◽  
Yuandani
Keyword(s):  
Acute Toxicity ◽  
Blood Cells ◽  
Hematological Parameters ◽  
White Blood Cells ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Female Rats ◽  
Control Group ◽  
Mean Corpuscular Hemoglobin ◽  
Corpuscular Hemoglobin

Traditionally pirdot leaves are used to treat various diseases. The purpose of this study was to determine determine the potential for acute toxicity of ethanolic extract of pirdot leaf (Saurauia vulcani Korth.) with value LD50 and hematological Parameters in rats. The acute toxicity of ethanolic extract of pirdot leaf was evaluated by OECD guidelines. The number of animals used in this research were 15 female rats. The control group was given Na CMC 0.5%, the treatment groups were given ethanolic extract of pirdot leaves with doses 2000 and 5000 mg/kg bw. The results showed that ethanolic extract of pirdot leaves with doses of 2000 and 5000 mg/kg bw did not show any toxicity signs. There was no mortality was observe. The ethanolic extract of pirdot leaves did not cause any changes in hematological parameters, these include red blood cells (RBC), hemoglobin, hematocrit, mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC), platelet, white blood cells (WBC), neutrophils, lymphocytes, monocytes, eosinophils, and basophils levels as compared to normal control (P>0.05). It was estimated that LD50 of ethanolic extract of pirdot leaves was higher than 5000 mg/kg bw and the extract were practically non-toxic. The ethanolic extract of pirdot leaves did not cause any toxic effect on hematological parameters.

Pesticides Residues in Blood Serum and Their Adverse Effects on Multi- Biomarkers in Occupationally Exposed Greenhouse Workers: A Cross-Sectional Study in Hamadan City, Iran

2021 ◽  
Author(s):  
Mostafa Leili ◽  
Abolfazl Ghafouri Khosrowshahi ◽  
Jalal Poorolajal ◽  
Fateme Samiee ◽  
Mohammad Taghi Samadi ◽  
...  
Keyword(s):  
Blood Serum ◽  
Crop Production ◽  
Hematological Parameters ◽  
Severe Form ◽  
Mean Corpuscular Hemoglobin ◽  
Adult Males ◽  
Corpuscular Hemoglobin ◽  
Cross Sectional ◽  
Pesticides Residues ◽  
Greenhouse Workers

Abstract The cultivation of greenhouse crops is the most severe form of crop production in Iran, and vast quantities of unreasonable pesticides are being utilized to control pests. The residual level of blood pesticides and its correlation with multi- biomarkers were determined to evaluate the adverse health consequences on greenhouse workers in the Hamdan city of Iran. Participants were 180 adult males, including 90 greenhouse workers exposed to pesticides and 90 control individuals. The residue analyses revealed that 64 out of the 90 workers had residues of dichlorvos, diazinon, and chlorpyrifos in their blood serum; and 56.25% out of these workers were exposed for > 15 years period. The residues of all three pesticides were higher than the no observable adverse effect level (NOAEL) in all the exposure duration categories. Comparisons of hematological parameters showed that mean monocyte (P < 0.001), lymphocyte (P < 0.001) and platelet counts (P = 0.001), mean platelet volume (P = 0.002), and plateletcrit (PCT) (P < 0.001) were significantly higher in workers than in controls, whereas hemoglobin (HB) (P = 0.028), mean corpuscular hemoglobin (MCH), and mean corpuscular hemoglobin concentrations (MCHC) (P < 0.001) were lower in workers than controls. These results show that exposure to different pesticides was associated with alterations in some biochemical and hematological parameters. Prevention and intervention programs can be effective in eliminating pesticide-related alterations among agricultural workers.

Variation in blood count parameters of children aged 5 to 11 years in Abidjan, Côte d'Ivoire

2021 ◽  
Author(s):  
Kokore Angoua Baudouin ◽  
Kamagate Soualio ◽  
Ouattara Howélé ◽  
Bleyere Nahounou Mathieu ◽  
Yapo Paul
Keyword(s):  
Reference Values ◽  
Cote D'ivoire ◽  
Mean Corpuscular Volume ◽  
Blood Count ◽  
Côte D’Ivoire ◽  
Hematological Parameters ◽  
Mean Corpuscular Hemoglobin ◽  
Corpuscular Hemoglobin ◽  
Cote D’Ivoire ◽  
Côte D'ivoire

Background: In Côte d’Ivoire, as in most developing countries, the reference values of hematological indices currently in use come from data collected from populations living in industrialized countries. The aim of this study was to determine variations in the child's blood count in Cote d'Ivoire. Materials and Methods: This descriptive cross-sectional study has focused on 310 children (172 girls and 138 boys) aged 5 to 11 years selected from three municipalities of Abidjan. Blood samples were taken from each child in order to assess the parameters of the blood count. Results: The results revealed that the means of the different hematological parameters (red blood cells, hemoglobin, hematocrit, white blood cells, neutrophils, eosinophils, monocyte, mean corpuscular volume, mean corpuscular hemoglobin and mean corpuscular hemoglobin concentration) were in accordance with the normal physiological reference values from the literature apart lymphocyte. The rate of lymphocytes (51.3 ± 0.5) was higher overall. All the parameters did not indicate significant differences between girls and boys (p > 0.05) except mean corpuscular volume, and mean corpuscular hemoglobin that were statistically different by sex (p < 0.05). The percentage of having a rate of haemoglobin lower than 11.5 decreased significantly (p˂0.005) when the age increased. In addition, the rate of hemoglobin and hematocrit increased slightly starting from the age of 8 years. There is a significant difference (p < 0.05) in hemoglobin level between girls and boys in the age group of 9 years. Leukopenia is observed in a minority of children in the age groups of 5, 6, 7, 8, and 10 years. Conclusion: Some hematological parameters were different from other data published in the literature, which suggested a local development of reference values for children.

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