scholarly journals Enzymolysis Technology Optimization for Production of Antioxidant Peptides from Goat Milk Casein

2017 ◽  
Vol 21 (1) ◽  
pp. 51-60 ◽  
Author(s):  
Guowei Shu ◽  
Zhuo Wang ◽  
Li Chen ◽  
Qian Zhang ◽  
Ni Xin
Keyword(s):  
Free Radicals ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Casein Hydrolysate ◽  
Goat Milk ◽  
Process Conditions ◽  
Scavenging Activity ◽  
Antioxidant Peptides ◽  
Hydrolysis Time ◽  
Milk Casein

AbstractAntioxidant peptides can inhibit lipid peroxidation and scavenging free radicals, maintain the balance of free radicals, and against a variety of diseases. Response surface methodology was used to optimize process conditions for producing antioxidative peptides from goat’s milk casein hydrolysate with Alcalase. The results suggested that the optimal process parameters were: temperature at 62.5°C, pH 8.9, E/S ration at 2.5%, substrate concentration at 4.4% and hydrolysis time was 173min). Metal-chelating effect, superoxide anion radical scavenging activity and 1, 1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging activity were shown to be 87.21±0.88%, 49.18±1.42% and 69.07±1.26% respectively under the optimal condition. The actual and predicated value were closely which indicated the optimized data fit well to model and the optimized parameters are reliable.

scholarly journals Effect of Temperature, pH, Enzyme to Substrate Ratio, Substrate Concentration and Time on the Antioxidative Activity of Hydrolysates from Goat Milk Casein by Alcalase

2016 ◽  
Vol 20 (2) ◽  
pp. 29-38 ◽  
Author(s):  
Guowei Shu ◽  
Bowen Zhang ◽  
Qian Zhang ◽  
Hongchang Wan ◽  
Hong Li
Keyword(s):  
Substrate Concentration ◽  
Superoxide Radical ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Goat Milk ◽  
Scavenging Activity ◽  
Antioxidant Peptides ◽  
Hydrolysis Time ◽  
Hydrolysis Temperature ◽  
Milk Casein

Abstract The effect of hydrolysis temperature (45, 50, 55, 60 and 65°C), pH (7.0, 7.5, 8.0, 8.5 and 9.0), enzyme to substrate (E/S) ratio (1.0, 1.5, 2.0, 2.5 and 3.0%), substrate concentration (2, 3, 4, 5 and 6%) and hydrolysis time (30-240min) on antioxidant peptides hydrolysated from goat’s milk casein by Alcalase was investigated using single factor experiment. In order to obtain high DPPH radical-scavenging activity, metal-chelating activity and superoxide radical scavenging activity, the optimal conditions were hydrolysis time of 150 min, temperature of 50°C, pH 8.0, E/S ratio of 2.0% and substrate concentration of 4.0%. The hydrolysis time, hydrolysis temperature, pH, E/S ratio and substrate concentration had a significant influence on degree of hydrolysis, metal-chelating activity, DPPH and superoxide radical scavenging activity on casein hydrolysate of goat milk by Alcalase, the results were beneficial for further provide theoretical basis for production of antioxidant peptides.

scholarly journals Effect Of Five Proteases Including Alcalase, Flavourzyme, Papain, Proteinase K And Trypsin On Antioxidative Activities Of Casein Hydrolysate From Goat Milk

2015 ◽  
Vol 19 (2) ◽  
pp. 65-74 ◽  
Author(s):  
Guowei Shu ◽  
Qian Zhang ◽  
He Chen ◽  
Hongchang Wan ◽  
Hong Li
Keyword(s):  
Superoxide Radical ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Casein Hydrolysate ◽  
Goat Milk ◽  
The Body ◽  
Proteinase K ◽  
Scavenging Activity ◽  
Dpph Scavenging ◽  
Casein Hydrolysates

Abstract Oxidation was related to the pathogenesis of human diseases. Adequate intake of antioxidant activity of food can reduce the levels of free radicals, prevent lipid peroxidation, and help the body against diseases. In the paper, casein from goat milk was hydrolyzed by five commercial proteases, namely, Alcalase, flavourzyme, papain, proteinase K and trypsin. The antioxidant activities of casein hydrolysates were assessed by evaluating hydrolysis degree, DPPH radical-scavenging activity, metal-chelating activity and superoxide radical scavenging activity. The results showed as follows: the DH of proteinase K, Alcalase, and trypsin were higher significantly than those of papain and flavourzyme. The Fe2+-chelation activity and superoxide radical scavenging activity of casein hydrolysates from goat milk by Alcalase was higher than the others, the DPPH scavenging activities of casein hydrolysates by Alcalase and papain was higher than the others and the DPPH scavenging activities by Alcalase and papain had no significant diffierence (p<0.05), so the optimal proteinase for hydrolysis casein from goat milk to produce antioxidant peptide was Alcalase.

scholarly journals Free Radical Scavenging Activity of Fruit Formulation Mediated Zinc Oxide Nanoparticles

2020 ◽  
Vol 11 (3) ◽  
pp. 4690-4694
Author(s):  
Tahoora Taskeen. L ◽  
Hannah R ◽  
Rajeshkumar S
Keyword(s):  
Antioxidant Activity ◽  
Zinc Oxide ◽  
Free Radicals ◽  
Zinc Oxide Nanoparticles ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Punica Granatum ◽  
Free Radical Scavenging ◽  
Oxide Nanoparticles ◽  
Scavenging Activity

To determine the free radical scavenging activity of fruit formulation mediated zinc oxide nanoparticle. Zinc oxide nanoparticles are widely known for their antimicrobial agent, anti-diabetic, anti-inflammatory, wound healing, antioxidant and optic properties. Oxidative degradation of lipids, proteins, food and cosmetics involves a radical-chain reaction with release of free radicals. These free radicals are extremely reactive and can even attack relatively stable molecules like the DNA bases. With a recent rise in diseases associated with increased oxidative stress, a good antioxidant seems to be the need of the hour. Synthesis of zinc oxide nanoparticles using Punica granatum and Elettaria cardamomum extract. Characterisation of the nanoparticles using UV–Visible spectroscopy and Scanning electron microscope. It is followed by the DPPH radical scavenging assay to determine the antioxidant activity. With an increase in the concentration of zinc oxide nanoparticles, there was an increase in the percentage of inhibition. When compared to the standard, the zinc oxide nanoparticles showed increased antioxidant activity even in small concentrations. Punica granatum and Elettaria cardamomum mediated Zinc oxide nanoparticles showed considerable antioxidant property even in small quantity when compared with standard vitamin C. Further studies need to be carried out to check for its potency as a prophylactic agent.

scholarly journals Probiotic and Antioxidant Potential of Lactobacillus reuteriLR12 and Lactobacillus lactisLL10 Isolated from Pineapple Puree and Quality Analysis of Pineapple-Flavored Goat Milk Yoghurt during Storage

2020 ◽  
Vol 8 (10) ◽  
pp. 1461 ◽  
Author(s):  
Naif Abdullah Al-Dhabi ◽  
Mariadhas Valan Arasu ◽  
Ponnuswamy Vijayaraghavan ◽  
Galal Ali Esmail ◽  
Veeramuthu Duraipandiyan ◽  
...  
Keyword(s):  
Pathogenic Bacteria ◽  
Radical Scavenging Activity ◽  
Starter Culture ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Goat Milk ◽  
Quality Analysis ◽  
Scavenging Activity ◽  
Bacterial Strains ◽  
Adhesion Properties

In recent years, studies have focused on the therapeutic properties of probiotics to eliminate pathogenic microorganisms associated with various diseases. Lactobacilli are important probiotics groups that have been found to possess many health-promoting activities. This study was carried out to isolate LactobacillusreuteriLR12 and L. lactisLL10 from pineapple puree. The invitro analysis to evaluate probiotic characteristics of the isolated bacteria included survival in bile and acid tolerance. The cell-free supernatant of L. reuteri LR12 was effective against various pathogenic bacteria and fungi compared with L. lactisLL10. These two bacterial strains have strong anti-biofilm activity (100%) against Enterococcus faecalis, Staphylococcus aureus, and Bacillus cereus. The bacterial strains exhibited adhesion properties to HT-29 cells (human colorectal adenocarcinoma). These bacteria showed DPPH- (2,2-diphenyl-1-picryl-hydrazyl-hydrate) free radical scavenging activity, scavenging of hydroxyl radical activity, superoxide radical scavenging activity, and reducing power activity in the range of 72% ± 3%to 89.3% ± 1.7%, 64% ± 2.7%to 66.8% ± 1.5%, 59.8% ± 4.1% to 63.8% ± 2.1%, and 60.4% ± 1.8%to 66.1% ± 3.3%, respectively. Pineapple puree was used as the starter culture with milk for 2 days for yogurt preparation. Pineapple puree increased flavor and showed the physicochemical properties of yogurt. The finding of the sensory evaluation revealed no significant change compared with the control, except the appearance of yogurt. These findings show that Lactobacilli and pineapple puree have potential use in various probiotic preparations for the fermentation industry.

Set-style yoghurts made from goat milk bases fortified with whey protein concentrates

2019 ◽  
Vol 86 (3) ◽  
pp. 361-367 ◽  
Author(s):  
Evangelia Zoidou ◽  
Sofia Theodorou ◽  
Ekaterini Moschopoulou ◽  
Lambros Sakkas ◽  
Georgios Theodorou ◽  
...  
Keyword(s):  
Whey Protein ◽  
Thermophilic Bacteria ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Goat Milk ◽  
Storage Period ◽  
Whey Protein Concentrate ◽  
Scavenging Activity ◽  
And Storage ◽  
Dpph Radical Scavenging

AbstractThis research paper addresses the hypothesis that the fortification of goat milk base with whey protein concentrate (WPC) could affect both the textural and the biofunctional properties of set-style yoghurt. The effect of fortification of goat milk base with two different WPCs on thermophilic bacteria counts, proteolysis, physical and biofunctional properties of set-style yoghurts was studied at specific sampling points throughout a 4-week storage period. Fortification and storage did not influence thermophilic counts. Physical properties were affected significantly (P < 0.05) by the composition of the protein and the mineral fraction of the WPC but not by the storage. ACE-inhibitory activity was moderate in accordance to low lactobacilli counts and lack of proteolysis. DPPH-radical scavenging activity, Fe2+-chelating activity and superoxide scavenging activity were high. At 28 d an anti-inflammatory effect was observed, which was not affected by WPC addition.

scholarly journals Separation and Enrichment of Antioxidant Peptides from Whey Protein Isolate Hydrolysate by Aqueous Two-Phase Extraction and Aqueous Two-Phase Flotation

Foods ◽  
2019 ◽  
Vol 8 (1) ◽  
pp. 34 ◽  
Author(s):  
Bin Jiang ◽  
Jiaxin Na ◽  
Lele Wang ◽  
Dongmei Li ◽  
Chunhong Liu ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Liquid Chromatography ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Phase Extraction ◽  
Scavenging Activity ◽  
Antioxidant Peptides ◽  
Two Phase ◽  
Aqueous Two Phase Extraction

At present, peptides are separated by molecular exclusion chromatography and liquid chromatography. A separation method is needed in any case, which can be scaled up for industrial scale. In this study, aqueous two-phase extraction (ATPE) and aqueous two-phase flotation (ATPF) were applied to separate and enrich antioxidant peptides from trypsin hydrolysates of whey protein isolates (WPI). The best experimental conditions were investigated, and the results were evaluated using the 2,2′-Azinobis-(3-ethylbenzthiazoline-6-sulphonate) (ABTS) free radical scavenging activity of the peptides-per-unit concentration and the recovery rate (Y) of peptides in the top phase of both ATPE and ATPF. Under optimal conditions, the Y and ABTS free radical scavenging activity per unit concentration in top phase of ATPE could reach 38.75% and 12.94%, respectively, and in ATPF could reach 11.71% and 29.18%, respectively. The purified peptides were characterized by matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) and reversed-phase high-performance liquid chromatography (RP-HPLC). PeptideCutter and PeptideMass were applied to analyze and calculate the peptide sequencing. KILDKVGINYWLAHK, VGINYWLAHKALCSEK, and TPEVDDEALEKFDKALK sequences having antioxidant activity were detected in the top phase of ATPE, and VGINYWLAHKALCSEK, KILLDKVGINYWLAHK, ILLDKVGINYWLAHK, IIAEKTKIPAVFK, KIIAEKTKIPAVFK, and VYVEELKPTPEGDLEILLQK sequences having antioxidant activity were detected in the top phase of ATPF. In conclusion, antioxidant peptides were successfully separated from the WPI hydrolysate by ATPE and ATPF; compared with ATPE, ATPF has superior specificity in separating antioxidant peptides.

Isolation and identification of antioxidant peptides derived from whey protein enzymatic hydrolysate by consecutive chromatography and Q-TOF MS

2013 ◽  
Vol 80 (3) ◽  
pp. 367-373 ◽  
Author(s):  
Qiu-Xiang Zhang ◽  
Hui Wu ◽  
Yu-Fang Ling ◽  
Rong-Rong Lu
Keyword(s):  
Whey Protein ◽  
Superoxide Radical ◽  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Gel Filtration ◽  
Scavenging Activity ◽  
Antioxidant Peptides ◽  
Isolation And Identification ◽  
Tof Ms

To isolate and identify antioxidant peptides from enzymatically hydrolysed whey protein, whey protein isolate was hydrolysed by different protease (trypsin, pepsin, alcalase 2·4L, promatex, flavourzyme, protease N). The hydrolysate generated by alcalase 2·4L had the highest antioxidant activities on 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals, superoxide radicals and in a linoleic acid peroxidation system induced by Fe2+. The IC50 values of DPPH and superoxide radical scavenging activities of the hydrolysate decreased significantly (6·89 and 38·88%, respectively) after treatment with macroporous adsorption resin. Seven different peptides showing strong antioxidant activities were isolated from the hydrolysate using consecutive chromatographic methods including gel filtration chromatography and high-performance liquid chromatography. The molecular mass and amino acids sequences of the purified peptides were determined using a Quadrupole time-of-flight mass spectrometer (Q-TOF MS). One of the antioxidative peptides, Trp–Tyr–Ser–Leu, displayed the highest DPPH radical scavenging activity (IC50=273·63 μm) and superoxide radical scavenging activity (IC50=558·42 μm). These results suggest that hydrolysates from whey proteins are good potential source of natural antioxidants.

scholarly journals Modulation of rabbit muscle sarcoplasmic reticulum Ca2+-ATPase activity by novel quercetin derivatives

2013 ◽  
Vol 6 (1) ◽  
pp. 3-8 ◽  
Author(s):  
Dušan Blaškovič ◽  
Petronela Žižková ◽  
Filip Držík ◽  
Jana Viskupičová ◽  
Miroslav Veverka ◽  
...  
Keyword(s):  
Heart Failure ◽  
Free Radicals ◽  
Free Radical ◽  
Sarcoplasmic Reticulum ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Rabbit Muscle ◽  
Scavenging Activity ◽  
Quercetin Derivatives

Abstract Sarcoplasmic reticulum Ca2+-ATPase (SERCA) is the pump crucial for calcium homeostasis and its impairment results in pathologies such as myopathy, heart failure or diabetes. Modulation of SERCA activity may represent an approach to the therapy of diseases with SERCA impairment involvment. Quercetin is flavonoid known to modulate SERCA activity. We examined the effect of nine novel quercetin derivatives on the activity of the pump. We found that 5-morpholinohydroxypoxyquercetin, di(prenylferuoyl)quercetin, di(diacetylcaffeoyl)-mono-(monoacetylcaffeoyl)quercetin and monoacetylferuloylquercetin stimulated the activity of SERCA. On the contrary, monochloropivaloylquercetin, tri(chloropivaloyl)quercetin, pentaacetylquercetin, tri(trimethylgalloyl)quercetin and diquercetin inhibited the activity of the pump. To identify compounds with a potential to protect SERCA against free radicals, we assessed the free radical scavenging activity of quercetin derivatives. We also related lipophilicity, an index of the ability to incorporate into the membrane of sarcoplasmic reticulum, to the modulatury effect of quercetin derivatives on SERCA activity. In addition to its ability to stimulate SERCA, di(prenylferuloyl)quercetin showed excellent radical scavenging activity.

scholarly journals Pharmacognostic review of Astercantha longifolia (L.) Nees, & its possible use in prevention of COVID-19

2021 ◽  
Vol 6 (4) ◽  
pp. 231-236
Author(s):  
Shiva Prasad Mohanty ◽  
◽  
Kshirabdhi Tanaya Rautaray ◽  
Keyword(s):  
Free Radicals ◽  
Viral Infections ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Viral Disease ◽  
Free Radical Scavenging ◽  
Point Of View ◽  
Scavenging Activity ◽  
Pharmacological Activities ◽  
Annual Herb

Astercantha longifolia is an annual herb from Acanthaceae family found almost as weed throughout plainlands, all over India. It is very much important from Ayurveda point of view for its curative role in many disease conditions. It is called as Kokilakshya in Ayurveda & Talamakhanna in Unani system. Its importance in Ayurveda can be judged by its visibility in Samhitas & Nighantus. India is a young country with billion plus population & having second highest in COVID affected numbers. Being a viral disease, immunity boosting drugs are the need of the hour & Astercantha longifolia (L) Nees or Kokilakshya fits in this situation perfectly. It has many pharmacological activities and the best action is, its free radical scavenging activity. Viral infections unleash storms of cytokinin, which produces abundant free radicals & the complications of COVID 19 are mainly due to these free radicals [1] . So, keeping all these qualities in mind, an honest attempt is made to enumerate those details and justify its perspective utility in Covid 19.

Free radicals scavenging aptitude of Spilanthes acmella

2021 ◽  
pp. 2631-2634
Author(s):  
K. Gouri Sankar ◽  
B. S. Venkateswarlu
Keyword(s):  
Antioxidant Activity ◽  
Free Radicals ◽  
Free Radical ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Free Radical Scavenging Activity ◽  
Bioactive Molecules ◽  
Scavenging Activity ◽  
Spilanthes Acmella

Now a days, oxidative stress is one of the major disease around the world. The excess production of oxidants in the body are key source for development of different chronic diseases and are leading to damage the multiple organs' function, finally to cellular mortality. The natural remedy is the most important medicine to control the overproduction of oxidants and to obtain more antioxidants. Natural product have been using to treat diseases and as food supplements as nutrients. In this point of view, the current study was carried to evaluate the antioxidant activity of Spilanthes acmella root parts. The free radical scavenging activity was studied against 2,2-diphenyl-1-picrylhydrazyl (DPPH), superoxide and hydroxyl free radicals using standard methods. The Spilanthes acmella root part extracts showed concentration dependent antioxidant activity on tested free radicals. The extracts showed modest effect on free radicals compared to the standard drug ascorbic acid and hydro-alcoholic extract possesses more activity than other two extracts. The end results of current study clearly state that Spilanthes acmella root possess free radical scavenging activity and also confirms its' traditional value in traditional medicine. The further research is worthful on isolation of different bioactive molecules and different biological activities evaluation on Spilanthes acmella.

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