Using in-situ viscosimetry and morphogranulometry to explore hydrolysis mechanisms of filter paper and pretreated sugarcane bagasse under semi-dilute suspensions

AbstractThe high cost of cellulolytic enzymes used in the ethanol production process has led to a growing interest in situ production. The evaluation of the influence of several factors in the fungus Penicillium sp. cellulase production using pretreated sugarcane bagasse is very interesting. Penicillium sp. cellulase production by using filter paper as cellulosic substrate and the use of glucose, sucrose and lactose like co-substrates was assessed. In the experiments using filter paper as a cellulosic substrate, the highest FPase enzyme activity obtained was 280 FPU.L-1 using sucrose as co-substrate. Subsequently, the study of pretreated sugarcane bagasse was conducted using Plackett-Burman experimental design with analysis of 6 factors influencing the process. The highest FPase activity was 615.1 FPU.L-1. The factors influencing FPase and β- glucosidase activity were the use of molasses and the solid loading. The successful use of molasses as co-substrate opens perspectives for future researches.

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Influence of the Chemical Composition on the Enzymatic Hydrolysis of Hot Water and Organosolv Pretreated Sugarcane Bagasse

Waste and Biomass Valorization ◽

10.1007/s12649-019-00657-2 ◽

2019 ◽

Vol 11 (7) ◽

pp. 3337-3344

Author(s):

María E. Vallejos ◽

Marcia D. Zambon ◽

María C. Area ◽

Antonio A. S. Curvelo

Keyword(s):

Chemical Composition ◽

Enzymatic Hydrolysis ◽

Sugarcane Bagasse ◽

Hot Water ◽

Pretreated Sugarcane Bagasse ◽

Hydrolysis Of

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Treated sugarcane bagasse for maintenance of sheep in Brazil

Proceedings of the British Society of Animal Science ◽

10.1017/s1752756200593673 ◽

1996 ◽

Vol 1996 ◽

pp. 172-172

Author(s):

A.L. Abdalla ◽

D.M.S.S. Vitti ◽

N.L. del Mastro ◽

M.S. Bueno

Keyword(s):

Body Weight ◽

Sugarcane Bagasse ◽

Present Experiment ◽

Dry Matter ◽

Weight Changes ◽

Corn Straw ◽

Microbial Synthesis ◽

Body Weight Changes ◽

Wool Production

Several treatments for increasing digestibility of sugarcane bagasse (SCB) have been studied in Brazil for many years (Abdalla et al., 1990). Despite some improvements in in situ digestibility obtained with steam and pressure treatments, the SCB has not performed well when given to cattle (Mello Junior, et al., 1989). More recently, irradiation and ammonia has been tested in studies to improve the digestibility of SCB, rice and corn straw, rice and soybean hulls and others rough feed. The purpose of the present experiment was aimed to determine the intake, body weight changes, wool production and microbial synthesis in sheep fed with grass hay (Cynodon dactilon) or SCB treated with 600kGy of radiation and ammonia (20g/kg dry matter (DM)).

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SYNCHRONIZATION OF CARBOHYDRATE AND PROTEIN SUPPLY IN THE SUGARCANE BAGASSE BASED RATION ON IN SITU NUTRIENT DEGRADABILITY

Journal of the Indonesian Tropical Animal Agriculture ◽

10.14710/jitaa.41.1.28-36 ◽

2016 ◽

Vol 41 (1) ◽

pp. 28-36 ◽

Cited By ~ 2

Author(s):

F. F. Sani ◽

L. K. Nuswantara ◽

E. Pangestu ◽

F. Wahyono ◽

J. Achmadi

Keyword(s):

Sugarcane Bagasse ◽

Crude Protein ◽

Degradation Kinetics ◽

Nitrogen Supply ◽

Neutral Detergent Fiber ◽

Synchronization Index ◽

In Situ Degradation ◽

Protein Supply ◽

Kinetics Of

Two adult male sheeps fitted with rumen cannula were used in two experiments to study the effects of synchronization of carbohydrate and nitrogen supply in sugarcane bagasse based total mixed ration (TMR) on in situ nutrient degradation. The first experiment was aimed to create three TMR with different synchronization index. Ingredient feedstuffs of TMR were evaluated for its in situ organic matter (OM) and nitrogen (N) degradation kinetics. On the basis of the OM and N degradation kinetics of feedstuffs, three sugarcane bagasse based TMR were formulated with synchronization indexes of 0.37; 0.50; and 0.63; respectively. The TMR had similar levels of crude protein (CP), total digestible nutrients, and neutral detergent fiber (NDF). In the second experiment, the three TMR with different synchronization index were evaluated for in situ degradability characteristics of OM, CP, NDF, and sulfur. The in situ degradation of OM in TMR were decreased (P<0.05) with the increasing of synchronization index. The higher synchronization index in TMR increased (P<0.05) CP degradation of CP. The NDF degradation decreased slightly by the alteration of synchronization index in TMR. The higher synchronization index in TMR reduced (P<0.05) in situ sulfur degradation, and this may not support to effects of synchronization of carbohydrate and nitrogen supply.

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Production of cellulases by solid state fermentation using natural and pretreated sugarcane bagasse with different fungi

Biocatalysis and Agricultural Biotechnology ◽

10.1016/j.bcab.2018.10.019 ◽

2019 ◽

Vol 17 ◽

pp. 1-6 ◽

Cited By ~ 14

Author(s):

Gabriella Soares Borges Salomão ◽

Jacyele Clarindo Agnezi ◽

Larissa Bastos Paulino ◽

Luana Borchardt Hencker ◽

Taísa Shimosakai de Lira ◽

...

Keyword(s):

Solid State ◽

Sugarcane Bagasse ◽

Solid State Fermentation ◽

Pretreated Sugarcane Bagasse

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On-site integrated production of cellulases and β-glucosidases by Trichoderma reesei Rut C30 using steam-pretreated sugarcane bagasse

10.1101/461012 ◽

2018 ◽

Cited By ~ 3

Author(s):

Marcella Fernandes de Souza ◽

Elba Pinto da Silva Bon ◽

Ayla Sant’ Ana da Silvab

Keyword(s):

Carbon Source ◽

Lignocellulosic Biomass ◽

Trichoderma Reesei ◽

Sugarcane Bagasse ◽

Enzyme Preparation ◽

Integrated Approach ◽

Aspergillus Awamori ◽

Pretreated Sugarcane Bagasse ◽

Hydrolysis Of ◽

Rut C30

AbstractThe high cost of commercial cellulases still hampers the economic competitiveness of the production of fuels and chemicals from lignocellulosic biomasses. This cost may be decreased by the on-site production of cellulases with the integrated use of the lignocellulosic biomass as carbon source. This integrated approach was evaluated in the present study whereby steam-pretreated sugarcane bagasse (SPSB) was used as carbon source for the production of cellulases by Trichoderma reesei Rut C30 and the produced enzymes were subsequently used for SPSB hydrolysis. An enzyme preparation with a high cellulase activity, of 1.93 FPU/mL, was obtained, and a significant β-glucosidase activity was achieved in buffered media, indicating the importance of pH control during enzyme production. The hydrolysis of SPSB with the laboratory-made mixture resulted in a glucose yield of 80%, which was equivalent to those observed for control experiments using commercial enzymes. Even though the supplementation of this mixture with external β-glucosidase from Aspergillus awamori was found to increase the initial hydrolysis rates, it had no impact on the final hydrolysis yield. It was shown that SPSB is a promising carbon source for the production of cellulases and β-glucosidases by T. reesei Rut C30 and that the enzyme preparation obtained is effective for the hydrolysis of SPSB, supporting the on-site integrated approach to decrease the cost of the enzymatic hydrolysis of lignocellulosic biomass.

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