scholarly journals The number of regulatory B cells is increased in mice with collagen-induced arthritis

2019 ◽  
Vol 14 (1) ◽  
pp. 12-18 ◽  
Author(s):  
Li Luo ◽  
Qing Liu ◽  
Shanshan Peng ◽  
Yan Meng ◽  
Wenjing Du ◽  
...  
Keyword(s):  
B Cells ◽  
Transforming Growth Factor ◽  
Interleukin 10 ◽  
Control Group ◽  
Synovial Cells ◽  
Regulatory B Cells ◽  
Collagen Induced Arthritis ◽  
Array Technology ◽  
Hematoxylin And Eosin ◽  
High Degree

AbstractThe aim of this study is to investigate changes in regulatory B cells (Bregs) and the expression of related cytokines such as interleukin-10 (IL-10) and transforming growth factor (TGF)-β in a mouse model of collagen-induced arthritis (CIA). A total 20 DBA/1 mice (6-8 weeks old) were randomly divided into control and CIA disease groups. For the CIA disease group, animals were injected intradermally with chicken collagen type II and complete Freund's adjuvant. The calculated arthritis index score of the CIA group was significantly higher than that in control group. Hematoxylin and eosin staining showed tumid synovial cells with irregular arrangement and obvious hyperplasia, with a high degree of inflammatory cell infiltration in CIA model group. Cytometric bead array technology and quantitative RT-PCR indicated that the levels of IL-10 and TGF-β in serum, and synovial cells were significantly increased in the CIA group. The proportion of Bregs in the spleen of the CIA group was significantly increased compared to the control group. In conclusion, our findings demonstrate that the number of Bregs and the expression of TGF-β and IL-10 are enhanced in mice with CIA.

scholarly journals The Roles of Regulatory B Cells in Cancer

2014 ◽  
Vol 2014 ◽  
pp. 1-7 ◽  
Author(s):  
Yan He ◽  
Hongyan Qian ◽  
Yuan Liu ◽  
Lihua Duan ◽  
Yan Li ◽  
...  
Keyword(s):  
B Cells ◽  
Immune Cells ◽  
Transforming Growth Factor Beta ◽  
Transforming Growth Factor ◽  
Autoimmune Disorder ◽  
Interleukin 10 ◽  
Regulatory B Cells ◽  
Experimental Conditions ◽  
Growth Factor Beta ◽  
Cytokines Secretion

Regulatory B cells (Bregs), a newly described subset of B cells, have been proved to play a suppressive role in immune system. Bregs can inhibit other immune cells through cytokines secretion and antigen presentation, which give them the role in the pathogenesis of autoimmune diseases and cancers. There are no clear criteria to identify Bregs; different markers were used in the different experimental conditions. Massive researches had described the functions of immune cells such as regulatory T cells (Tregs), dendritic cells (DCs), and B cells in the autoimmune disorder diseases and cancers. More and more researches focused on the roles of Bregs and the cytokines such as Interleukin-10 (IL-10) and transforming growth factor beta (TGF-β) secreted by Bregs. The aim of this review is to summarize the characteristics of Bregs and the roles of Bregs in cancer.

scholarly journals Research Progress on Effects of Regulatory B Cells in Infection Immunity

2016 ◽  
Vol 5 (1) ◽  
pp. 23-26
Author(s):  
Ting Miao
Keyword(s):  
B Cells ◽  
Transforming Growth Factor ◽  
Viral Infections ◽  
Inflammatory Responses ◽  
Interleukin 10 ◽  
Transforming Growth Factor Β1 ◽  
Research Progress ◽  
Regulatory B Cells ◽  
Related Research ◽  
Regulatory Functions

Abstract B cells play immunomodulatory roles mainly by presenting antigens and producing antibodies. In recent years, some B cells were shown to exhibit regulatory functions. This type of B cell was named regulatory B cells (Bregs). Bregs can mediate immune tolerance to inhibit excessive inflammatory responses and to accelerate recovery of inflammation by producing interleukin 10 and/or transforming growth factor β1 and other inhibitory cytokines. Studies showed that Bregs play important roles in parasites, bacteria, and viral infections. This study reviews biological characteristics, functions, and microsignal regulation of Bregs and their mechanism in infectious diseases and related research progress.

scholarly journals The Potential Role of Regulatory B Cells in Idiopathic Membranous Nephropathy

2020 ◽  
Vol 2020 ◽  
pp. 1-12
Author(s):  
Zhaocheng Dong ◽  
Zhiyuan Liu ◽  
Haoran Dai ◽  
Wenbin Liu ◽  
Zhendong Feng ◽  
...  
Keyword(s):  
Immune Response ◽  
B Cells ◽  
Membranous Nephropathy ◽  
Transforming Growth Factor ◽  
Transforming Growth Factor Β ◽  
Interleukin 10 ◽  
Autoimmune Response ◽  
Idiopathic Membranous Nephropathy ◽  
Regulatory B Cells

Regulatory B cells (Breg) are widely regarded as immunomodulatory cells which play an immunosuppressive role. Breg inhibits pathological autoimmune response by secreting interleukin-10 (IL-10), transforming growth factor-β (TGF-β), and adenosine and through other ways to prevent T cells and other immune cells from expanding. Recent studies have shown that different inflammatory environments induce different types of Breg cells, and these different Breg cells have different functions. For example, Br1 cells can secrete IgG4 to block autoantigens. Idiopathic membranous nephropathy (IMN) is an autoimmune disease in which the humoral immune response is dominant and the cellular immune response is impaired. However, only a handful of studies have been done on the role of Bregs in this regard. In this review, we provide a brief overview of the types and functions of Breg found in human body, as well as the abnormal pathological and immunological phenomena in IMN, and propose the hypothesis that Breg is activated in IMN patients and the proportion of Br1 can be increased. Our review aims at highlighting the correlation between Breg and IMN and proposes potential mechanisms, which can provide a new direction for the discovery of the pathogenesis of IMN, thus providing a new strategy for the prevention and early treatment of IMN.

P0523PROTECTIVE ROLE OF REGULATORY B CELLS ON THE RENAL TISSUE REPAIRMEN AFTER ISCHEMIA REPERCUSSION INJURY

2020 ◽  
Vol 35 (Supplement_3) ◽  
Author(s):  
Yokota Yunosuke ◽  
Goh Kodama ◽  
Sakuya Itou ◽  
Yosuke Nakayama ◽  
Nobukazu Komatsu ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Renal Function ◽  
B Cells ◽  
Interleukin 10 ◽  
Renal Tissue ◽  
Protective Role ◽  
Regulatory B Cells ◽  
Tubulointerstitial Injury ◽  
Iri Model

Abstract Background and Aims Acute kidney injury (AKI), even if followed by renal recovery, is a risk factor for the future development of chronic kidney disease (CKD) and end- stage renal disease. It has been postulated that interleukin-10 (IL-10)-producing Regulatory B cells (Breg) play an important role for the tissue repairment in several tissues and organs. Basically, protective role of Breg has been reported in inflammatory bowel disease. In the kidney, it has been shown that IL-10 suppresses renal function decline and improves renal prognosis in IRI model, a typical model of AKI. However, the identity of Breg in the kidney and their origin have not been clarified. Further, how the Breg works during the transition from AKI to CKD is not known. Therefore, first we investigated whether Breg existed in renal tissue on the progression from AKI to CKD in IRI model mice. Further, we performed splenectomy, and examined the renal injury, Breg, and plasma IL-10 levels in this model. Method To examine the existence of Breg in the kidney of IRI model, we used 8-10 weeks-old GFP / IL-10 mice based on C57BL / 6J mice. They are reporter mice for IL-10 producing cells, and can visualize IL-10 producing cells under a fluorescence microscope without fluorescent immunostaining. We prepared following three groups, sham, IRI (unilateral), and IRI + SN (splenectomy) groups. Mice were anesthetized with chloral hydrate (4 g/kg,, intraperitoneal). After making a midline incision, exposed a blood vessel of the left renal pedicles and clamped it for 30 min by clips. one day, 7 days, and 14 days after the surgery, mice were sacrificed, and renal function and plasma IL-10 levels as well as tissue damages by PAS and Masson’s Trichrome staining were assessed. Tissue IL-10-producing cells were detected by flow cytometry. Results There was no difference of plasma IL-10 levels and renal tubulointerstitial injury in IRI group and IRI+SN group on day 1 after IRI. However, on day 7 and day 14, plasma IL-10 levels became gradually higher in IRI group, and SN decreased the increase in IL-10 levels. Tubulointerstitial injury was induced by IRI and SN further worsened tubular damages. Serum Cr and BUN levels were not different in three groups due to normal right kidney. On day 1, number of IL-10-producing B cells increased in the spleen and renal medulla in IRI group confirmed by flow cytometry, which was completely diminished by SN, suggesting that origin of the infiltrated Breg might be spleen, thereby being involved in the protective role in IRI injury in the kidney. Conclusion We report for the first time that Breg might be recruited from spleen by AKI, which may be one of the mechanisms to prevent the progression to CKD.

Interleukin-10 expressing regulatory B cells are decreased in blood of smokers and COPD patients

2021 ◽  
Author(s):  
Merel Jacobs ◽  
Sven Verschraegen ◽  
Bihiyga Salhi ◽  
Guy Brusselle ◽  
Ken Bracke
Keyword(s):  
B Cells ◽  
Interleukin 10 ◽  
Regulatory B Cells ◽  
Copd Patients

scholarly journals Potential Role for Regulatory B Cells as a Major Source of Interleukin-10 in Spleen fromPlasmodium chabaudi-Infected Mice

2018 ◽  
Vol 86 (5) ◽  
Author(s):  
Xue Han ◽  
Ji Yang ◽  
Yitong Zhang ◽  
Yalin Zhang ◽  
Hongtao Cao ◽  
...  
Keyword(s):  
Immune Response ◽  
B Cells ◽  
Potential Role ◽  
Interleukin 10 ◽  
Surface Markers ◽  
Regulatory B Cells ◽  
Plasmodium Chabaudi ◽  
Impact On Survival ◽  
And Function ◽  
Stimulation Of

ABSTRACTInterleukin-10 (IL-10)-producing regulatory B (Breg) cells were found to be induced in a variety of infectious diseases. However, its importance in the regulation of immune response to malaria is still unclear. Here, we investigated the dynamics, phenotype, and function of Breg cells usingPlasmodium chabaudi chabaudiAS-infected C57BL/6 and BALB/c mice. BALB/c mice were more susceptible to infection and had a stronger IL-10 response in spleen than C57BL/6 mice. Analysis of the surface markers of IL-10-producing cells with flow cytometry showed that CD19+B cells were one of the primary IL-10-producing populations inP. c. chabaudiAS-infected C57BL/6 and BALB/c mice, especially in the latter one. The Breg cells had a heterogeneous phenotype which shifted during infection. The well-established Breg subset, CD19+CD5+CD1dhicells, accounted for less than 20% of IL-10-producing B cells in both strains during the course of infection. Most Breg cells were IgG+and CD138−from day 0 to day 8 postinfection. Adoptive transfer of Breg cells to C57BL/6 mice infected withP. c. chabaudiAS led to a transient increase of parasitemia without an impact on survival rate. Our finding reveals that B cells play an active and important regulatory role in addition to mediating humoral immunity in immune response against malaria, which should be paid more attention in developing therapeutic or vaccine strategies against malaria involving stimulation of B cells.

scholarly journals Vasoactive intestinal peptide stabilizes intestinal immune homeostasis through maintaining interleukin-10 expression in regulatory B cells

Theranostics ◽  
2019 ◽  
Vol 9 (10) ◽  
pp. 2800-2811 ◽  
Author(s):  
Xiong Sun ◽  
Chuanyong Guo ◽  
Fang Zhao ◽  
Jianhuan Zhu ◽  
Yilu Xu ◽  
...  
Keyword(s):  
B Cells ◽  
Vasoactive Intestinal Peptide ◽  
Interleukin 10 ◽  
Immune Homeostasis ◽  
Regulatory B Cells

scholarly journals Lactobacillus sakei suppresses collagen-induced arthritis and modulates the differentiation of T helper 17 cells and regulatory B cells

2020 ◽  
Author(s):  
Jooyeon Jhun ◽  
Hong Ki Min ◽  
Jaeyoon Ryu ◽  
Seon-Yeong Lee ◽  
Jun-Geol Ryu ◽  
...  
Keyword(s):  
B Cells ◽  
Serum Level ◽  
Mononuclear Cells ◽  
Treated Group ◽  
Lactobacillus Sakei ◽  
Regulatory B Cells ◽  
T Helper ◽  
Collagen Induced Arthritis ◽  
T Helper 17 ◽  
T Helper 17 Cells

Abstract Background: To evaluate the immunomodulatory effect of Lactobacillus sakei in a mouse model of rheumatoid arthritis (RA) and in human immune cells. Methods: We evaluated whether L. sakei reduced the severity of collagen-induced arthritis (CIA) and modulated interleukin (IL)-17 and IL-10 levels, as well as whether it affected the differentiation of CD4 + T cells and regulatory B cells. We evaluated osteoclastogenesis after culturing bone marrow-derived mononuclear cells with L. sakei. Results: The differentiation of T helper 17 cells and the serum level of IL-17 were suppressed by L. sakei in both human peripheral blood mononuclear cells and mouse splenocytes. The serum level of IL-10 was significantly increased in the L. sakei -treated group, whereas the regulatory T cell population was unchanged. The population of regulatory B cells significantly increased the in L. sakei -treated group. Oral administration of L. sakei reduced the arthritis incidence and score in mice with CIA. Finally, osteoclastogenesis and the mRNA levels of osteoclast-related genes were suppressed in the L. sakei -treated group. Conclusion: L. sakei exerted an anti-inflammatory effect in an animal model of RA, regulated Th17 and regulatory B cell differentiation, and suppressed osteoclastogenesis. Our findings suggest that L. sakei has therapeutic potential for RA.

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