Supplementation of probiotics in water beneficial growth performance, carcass traits, immune function, and antioxidant capacity in broiler chickens

Abstract This study aims to investigate the effects of commercial probiotic supplementation in water on the performance parameters, carcass traits, immune function, and antioxidant capacity of broiler chicks. In the experiment, 120 Arbor Acres (AA) broilers (60 male and 60 female) were randomly allocated into four groups (G) – G1: basal diet and G2, G3, and G4: basal diet with 1% Lactobacillus casei, 1% L. acidophilus, and 1% Bifidobacterium in the water, lasting 42 days. The experimental results revealed that probiotic additives produced positive impacts on body weight, average daily feed intake (ADFI), and average daily weight gain for female chicks, whereas these probiotics significantly reduced ADFI and the feed conversion ratio of male chicks (P < 0.05). Probiotics efficiently improved eviscerated yield and breast yield while reducing the abdominal fat (P < 0.05) for the male broiler chicks. A marked increase was observed in the weight of the spleen, bursa of Fabricius, and thymus in the treatment group (P < 0.05). Besides, probiotics produced a significant effect on the concentrations of immune-related proteins (P < 0.05) and markedly increased the concentrations of antioxidase and digestive enzymes when compared with the control (P < 0.05). The addition of probiotics dramatically reduced the total counts of Escherichia coli and Salmonella and increased the quantity of Lactobacilli (P < 0.05). The results of the present study demonstrated an increase in growth performance, carcass traits, immune function, gut microbial population, and antioxidant capacity by supplementing 1% probiotics (L. casei, L. acidophilus, and Bifidobacterium) in the water for broilers.

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Effects of feeding Isomaltooligosaccharides on the growth performance, carcass traits and immune response of broiler chickens

JOURNAL OF ADVANCES IN AGRICULTURE ◽

10.24297/jaa.v3i2.4256 ◽

2014 ◽

Vol 3 (1) ◽

pp. 150-157

Author(s):

Khalid M. Gaafar

Keyword(s):

Immune Response ◽

Growth Performance ◽

Broiler Chickens ◽

Carcass Traits ◽

Dietary Supplementation ◽

Control Group ◽

Broiler Chicks ◽

Feed Conversion ◽

Flu Vaccine ◽

Lipids Profile

The research was conducted to study the effect of feeding broiler chickens on diets containing isomaltooligosaccharides on the growth performance, carcass traits and immune response. 90-one day old broiler chicks were used according to completely randomized two treatment groups and one control, 30 birds each. Birds fed ad-libitum on basal starter and grower-finisher diets for 35 day. Diets of treatment`s groups contained 0.5 g/Kg and 1 g/Kg of Isomaltooligosaccharides, while the control group fed on the basal diets without Isomaltooligosaccharides supplementation. Dietary supplementation of broiler chickens with Isomaltooligosaccharides improved body weight, feed conversion, carcass traits, two lymphoid organs weight and log antibody titer against avian flu vaccine. Most of the highest values were for birds fed low levels of Isomaltooligosaccharides. Feed intake decreases as Isomaltooligosaccharides level increases. Dietary supplementation with Isomaltooligosaccharides did not affect the lipids profile (triglycerides, total cholesterol, LDL and HDL), however the blood VLDL levels decreased with increased levels of Malondialdehyde and Glutathione reductase. Collectively, Dietary supplementation of broiler chickens with 0.5 g/Kg diet of Isomaltooligosaccharides improved growth performance, carcass traits and immune status.

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The Effect of Replacing Dried Meal Worm with Soybean Meal on Growth Performance and Carcass Characteristics of Broiler Chickens

10.21203/rs.3.rs-782326/v1 ◽

2021 ◽

Author(s):

Navid Naderi Boroojerdi ◽

Mostafa Rajabzadeh

Keyword(s):

Growth Performance ◽

Soybean Meal ◽

Broiler Chickens ◽

Relative Weight ◽

Carcass Characteristics ◽

Basal Diet ◽

Control Treatment ◽

Broiler Chicks ◽

Feed Conversion ◽

Significant Difference

Abstract An experiment was conducted on 240 one-day old male (Ross308) chicken to the effect of substitution of dried mealworm (Tenebrio molitor) with soybean meal on growth performance and carcass characteristics broiler chicks. Chicks fed in a completely randomized design with 4 replicate cages (12 birds per cage). Five dietary treatments used: 1- Control treatment: Basal diet based on corn-soybean meal without adding dried mealworm, 2- Replacement of 5% dried mealworm with soybean meal in the base diet, 3- Replacement of 10% dried mealworm with soybean meal in the base diet, 4- Replacement of 15% dried mealworm with soybean meal in the base diet and 5- Replacement of 20% dried mealworm with soybean meal in the base diet. The results of the experiment showed that 3rd and 4th treatments with a substitution of 10 and 15 percent showed a higher mean weight gain and a daily gain, but their feed intake was lower comparing other treatments, and finally, the feed conversion ratio showed a significant decrease with respect to control treatment using these replacement levels. At 21 and 42 days of age, carcass yield and relative weight of the breasts showed significant increases in treatments 3 and 4, and other characteristics of chicken carcasses were not affected by the test treatments. The experiment showed that replacing soybean meal with 10% and 15% worm powder significantly improved the performance of broiler chickens, but no significant difference was found between the two levels.

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Effects of Bacillus amyloliquefaciens LFB112 on Growth Performance, Carcass Traits, Immune, and Serum Biochemical Response in Broiler Chickens

Antibiotics ◽

10.3390/antibiotics10111427 ◽

2021 ◽

Vol 10 (11) ◽

pp. 1427

Author(s):

Marhaba Ahmat ◽

Junhao Cheng ◽

Zaheer Abbas ◽

Qiang Cheng ◽

Zhen Fan ◽

...

Keyword(s):

Growth Performance ◽

Bacillus Amyloliquefaciens ◽

Broiler Chickens ◽

Average Daily Gain ◽

Carcass Traits ◽

Serum Triglyceride ◽

Basal Diet ◽

Feed Conversion ◽

Vegetative Cells ◽

Serum Biochemical

This study aimed to investigate the effects of Bacillus amyloliquefaciens LFB112 on the growth performance, carcass traits, immune response, and serum biochemical parameters of broiler chickens. A total of 396 1 day old, mixed-sex commercial Ross 308 broilers with similar body weights were allotted into six treatment groups. The assigned groups were the CON group (basal diet with no supplement), AB (antibiotics) group (basal diet + 150 mg of aureomycin/kg), C+M group (basal diet + 5 × 108 CFU/kg B. amyloliquefaciens LFB112 powder with vegetative cells + metabolites), C group (basal diet + 5 × 108 CFU/kg B. amyloliquefaciens LFB112 vegetative cell powder with removed metabolites), M group (basal diet + 5 × 108 CFU/kg B. amyloliquefaciens LFB112 metabolite powder with removed vegetative cells), and CICC group (basal diet + 5 × 108 CFU/kg Bacillus subtilis CICC 20179). Results indicated that chickens in the C+M, C, and M groups had higher body weight (BW) and average daily gain (ADG) (p < 0.05) and lower feed conversion ratio (FCR) (p = 0.02) compared to the CON group. The C+M group showed the lowest abdominal fat rate compared to those in the CON, AB, and CICC groups (p < 0.05). Compared to the CON group, serum IgA and IgG levels in the C+M, C, and M groups significantly increased while declining in the AB group (p < 0.05). B. amyloliquefaciens LFB112 supplementation significantly reduced the serum triglyceride, cholesterol, urea, and creatinine levels, while increasing the serum glucose and total protein (p < 0.05). In conclusion, B. amyloliquefaciens LFB112 significantly improved the growth performance, carcass traits, immunity, and blood chemical indices of broiler chickens and may be used as an efficient broiler feed supplement.

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Effects of stale maize on growth performance, immunity, intestinal morphology and antioxidant capacity in broilers

Asian-Australasian Journal of Animal Sciences ◽

10.5713/ajas.19.0224 ◽

2020 ◽

Vol 33 (4) ◽

pp. 605-614 ◽

Cited By ~ 10

Author(s):

J. B. Liu ◽

H.L. Yan ◽

Y. Zhang ◽

Y.D. Hu ◽

H.F. Zhang

Keyword(s):

Antioxidant Capacity ◽

Growth Performance ◽

Interleukin 2 ◽

Relative Weight ◽

Basal Diet ◽

Interferon Γ ◽

Intestinal Morphology ◽

Bursa Of Fabricius ◽

Feed Conversion ◽

Factor Α

Objective: This study was conducted to determine the effects of stale maize on growth performance, immunity, intestinal morphology, and antioxidant capacity in broilers.Methods: A total of 800 one-day-old male Arbor Acres broilers (45.4±0.5 g) were blocked based on body weight, and then allocated randomly to 2 treatments with 20 cages per treatment and 20 broilers per cage in this 6-week experiment. Dietary treatments included a basal diet and diets with 100% of control maize replaced by stale maize.Results: The content of fat acidity value was higher (p<0.05) while the starch, activities of catalase and peroxidase were lower (p<0.05) than the control maize. Feeding stale maize diets reduced (p<0.05) average daily feed intake (ADFI) throughout the experiment, feed conversion ratio (FCR) during d 0 to 21 and the whole experiment as well as relative weight of liver, spleen, bursa of Fabricius and thymus (p<0.05) on d 21. Feeding stale maize diets decreased jejunum villus height (VH) and VH/crypt depth (CD) (p<0.05) on d 21 and 42 as well as ileum VH/CD on d 42. The levels of immunoglobulin G, acid α-naphthylacetate esterase positive ratios and lymphocyte proliferation on d 21 and 42 as well as lysozyme activity and avian influenza antibody H<sub>5</sub>N<sub>1</sub> titer on d 21 decreased (p<0.05) by the stale maize. Feeding stale maize diets reduced (p<0.05) serum interferon-γ, tumor necrosis factor-α, interleukin-2 on d 21 and interleukin-6 on d 21 and 42. Broilers fed stale maize diets had lower levels of (p<0.05) total antioxidative capacity on d 42, superoxide dismutase and glutathione peroxidase on d 21 and 42, but higher (p<0.05) levels of malondialdehyde on d 21 and 42.Conclusion: Feeding 100% stale maize decreased ADFI and FCR, caused adverse effects on immunity and antioxidant function and altered intestinal morphology in broilers.

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Effect of Garlic Oil Inclusion on Carcass Quality, Growth Performance and Cost Implication of Broiler Chickens

Sumerianz Journal of Agriculture and Veterinary ◽

10.47752/sjav.310.150.154 ◽

2020 ◽

pp. 150-154

Author(s):

Bamigboye Samson. O. ◽

Oluwafemi R. A. ◽

Maduekwe Innocent M.

Keyword(s):

Mortality Rate ◽

Growth Performance ◽

Broiler Chickens ◽

Basal Diet ◽

Control Treatment ◽

Carcass Quality ◽

Broiler Chicks ◽

Feed Conversion ◽

Cost Implication ◽

Garlic Oil

This study was carried to evaluate the “Effect of Garlic (Allium sativum) oil inclusion on carcass quality, growth performance and cost implication of broiler chickens”. A total of 180 day–old-(Arboacre) broiler chicks purchased from a reputable hatchery was used in the study which lasted for eight (8) weeks. The birds were divided into four experimental groups; each group was further subdivided into three replicates of fifteen birds per each in a Complete Randomized Design (CRD). The birds were fed on two basal diets (Starter and finisher) in an experiment which lasted for eight weeks. The garlic oil (Allum sativum) was added to the basal diet at (0.00, 0.1, 0.2, 0.3%) level respectively. The treatment were T1, T2, T3, and T4 with T1 serving as control. Growth performance, carcass characteristics, and cost implication were recorded. At the end of the experiment, the birds were slaughtered, dressed to estimate the different parameters. The result showed that the diet with 0.3% garlic oil inclusion had significantly (P<0.5) highest body weight and highest dressing percentage. Control treatment (0.0%) had highest feed intake and best feed conversion ratio. The mortality rate was not affected significantly by the addition of garlic oil in broiler diet. The garlic oil inclusion reduced the mortality rate of the birds, increases productivity and food safety. The result showed on cost implication reveal the economical evaluation of the experiment diets indicated that, the diet with 0.3% level of garlic oil showed the highest profitability ratio as compared to the group. This might be due to the highest return of the weight gains recorded by this group of chicken.

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Bovine testicular fluid enhanced growth performance of broiler chickens

Nigerian Journal of Animal Production ◽

10.51791/njap.v46i1.1287 ◽

2020 ◽

Vol 46 (1) ◽

pp. 188-195

Author(s):

E. O. Ewuola ◽

A. T. Olujimi

Keyword(s):

Growth Performance ◽

Broiler Chickens ◽

Serum Testosterone ◽

Conversion Ratio ◽

Feed Conversion Ratio ◽

Daily Weight Gain ◽

Enzyme Linked Immunosorbent Assay ◽

Broiler Chicks ◽

Growth Promoter ◽

Feed Conversion

Testosterone has been reported to have anabolic influence on various organs of animals. Thus, an experiment was carried out to investigate the influence of crude testosterone in bovine testicular fluid (BTF) on the growth performance of broiler chickens. One hundred and twenty-five broiler chicks (Abore Acre) were used. They were raised for one week and randomly allotted into five treatments consisted of five replicates (five chicks per replicate) under the intensive management system. Four different levels; 25mL, 50mL, 75mL, and 100mL of bovine testicular fluid per litre of water were administered to the birds, while the control birds were offered ordinary water. Feeds (broiler starter and finisher diets) were provided ad libitum on daily basis at weeks 2-4 and 5-8, respectively and clean water was given at free choice throughout the period of the study. Average daily feed intake (ADFI), average daily water intake (ADWI), average daily weight gain (ADWG) per bird, and the feed conversion ratio (FCR) of the birds were determined. At week seven blood was sampled to assay for level of the luteinizing hormone (LH), follicle stimulating hormone (FSH), and testosterone. The results indicated that ALW and the ADWG of the T5 birds (1850.00g/bird and 43.79g/bird, respectively) were significantly (p<0.05) higher than birds in T1, T2, T3, and T4, while the ADFI of T1, T3 and T4 birds (90.33g/bird, 94.09g/bird, 90.39g/bird respectively) were significantly (P < 0.05) higher than T2 (84.60g/bird) and T5 (84.60g/bird). ADWI of the T5 and T4 birds (101.01mL/bird, and 98.68mL/bird, respectively) were significantly (P < 0.05) higher than TI (82.13mL/bird), T2 (86.50mL/bird) and T3 (93.01mL/bird) birds. Birds administered 100mL of BTF/litre (T5) had the best percentage carcass yield and feed conversion ratio of 76.60% and 2.11 respectively, among the treatments T1, T2, T3, and T4 birds. The result of the enzyme-linked immunosorbent assay of the serum concentration of the sex hormones showed that the expression of the serum testosterone increased as the inclusion levels of the Bovine testicular fluid increased with birds on T5 having significantly (P < 0.05) higher values (3.64 nmol/L) than those on the control (2.60 nmol/L). However, testosterone levels in T2 (3.18 nmol/L), T3 (3.24 nmol/L) and T4 (3.40 nmol/L) was not significantly different from T5. This study suggests that 100ml of bovine testicular fluid per liter of the drinking water can be used as a growth promoter for broiler chickens.

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Protected Organic Acids Improved Growth Performance, Nutrient Digestibility, and Decreased Gas Emission in Broilers

Animals ◽

10.3390/ani10030416 ◽

2020 ◽

Vol 10 (3) ◽

pp. 416 ◽

Cited By ~ 1

Author(s):

Dinh Hai Nguyen ◽

In Ho Kim

Keyword(s):

Organic Acids ◽

Growth Performance ◽

Broiler Chickens ◽

Body Weight Gain ◽

Basal Diet ◽

Nutrient Digestibility ◽

Broiler Chicks ◽

Feed Conversion ◽

Gas Emission ◽

Dietary Treatments

We investigated the effects of a blend of organic acids (OAs) and medium-chain fatty acids (MCFAs) supplementation in 800 1-d-old male Ross 308 broiler chickens (42 ± 0.90 g) in a 7-week study. Broiler chicks were randomly allocated into one of the five dietary treatments (16 birds per pen with 10 pens per treatment). Dietary treatments consisted of corn-soybean meal based basal diet and the basal diet supplemented with blend of OAs and MCFAs at 0.25, 0.5, 0.75 g, and 1 g per kg of feed. In the current study, during the whole experimental period, the inclusion of the blend of OAs and MCFAs in the basal diet linearly improved (p < 0.05) body weight gain (BWG), feed conversion ratio (FCR), and dry matter digestibility. The increasing inclusion of the blend of OA and MCFA levels in the diets linearly decreased (p = 0.002) feed intake during d 1 to 7. Broilers fed diets containing different levels of the blend of OAs and MCFAs showed a linear increase (p = 0.006) in Lactobacillus concentrations and decrease (p = 0.014) in ammonia (NH3) at the end of the experiment. However, the blend of OAs and MCFAs did not affect carcass quality, E. coli, and Salmonella counts, as well as hydrogen sulfide and total mercaptans gas emission (p > 0.05). In conclusion, the blend of OA and MCFA supplementation positively influenced growth performance, DM digestibility, excreta Lactobacillus counts, as well as NH3 gas emission in broiler chickens.

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Effects of Bacillus megaterium on growth performance, serum biochemical parameters, antioxidant capacity, and immune function in suckling calves

Open Life Sciences ◽

10.1515/biol-2020-0106 ◽

2020 ◽

Vol 15 (1) ◽

pp. 1033-1041 ◽

Cited By ~ 1

Author(s):

Jun Yao ◽

Lili Wang ◽

Wenju Zhang ◽

Mengjian Liu ◽

Junli Niu

Keyword(s):

Antioxidant Capacity ◽

Immune Function ◽

Growth Performance ◽

Bacillus Megaterium ◽

Biochemical Parameters ◽

Basal Diet ◽

Daily Weight Gain ◽

Control Group ◽

Serum Biochemical Parameters ◽

Serum Biochemical

AbstractBackgroundThis study was conducted to investigate the effects of Bacillus megaterium on growth performance, serum biochemical parameters, antioxidant capacity, and immune function in suckling calves.MethodsIn total, 20 1-day-old Holstein calves with similar body weight (BW) and good health condition were randomly assigned into two groups with ten replicates per group and one calf per replicate. The control group (CON group) was fed a basal diet, whereas the B. megaterium group (BM group) was fed the basal diet supplemented with 500 mg/day/head of B. megaterium (1010 CFU/g) for 28 days.ResultsThe results revealed that the BM group showed an increase in final BW, daily weight gain, and feed-to-gain ratio (p < 0.05) and a decrease in diarrhea rate. Moreover, the concentrations of serum cholesterol and high-density lipoprotein decreased (p < 0.05) in the BM group compared with the CON group at 28 days. The level of serum glutathione was higher (p < 0.05) in the BM group than that of the CON group at 14 days, whereas the level of serum malondialdehyde decreased (p < 0.01) in the BM group compared with the CON group at 28 days. In addition, compared with the CON group (p < 0.05), the concentrations of serum IgA, IgM, IgG, and IL-4 were higher, whereas the concentration of serum TNF-α decreased in the BM group at 28 days.ConclusionB. megaterium had beneficial effects on the improvement of growth performance, immune function, and intestinal oxidative status of suckling calves.

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Supplementing Garlic Nanohydrogel Optimized Growth, Gastrointestinal Integrity and Economics and Ameliorated Necrotic Enteritis in Broiler Chickens Using a Clostridium perfringens Challenge Model

Animals ◽

10.3390/ani11072027 ◽

2021 ◽

Vol 11 (7) ◽

pp. 2027

Author(s):

Doaa Ibrahim ◽

Tamer Ahmed Ismail ◽

Eman Khalifa ◽

Shaimaa A. Abd El-Kader ◽

Dalia Ibrahim Mohamed ◽

...

Keyword(s):

Growth Performance ◽

Clostridium Perfringens ◽

Broiler Chickens ◽

Body Weight Gain ◽

Economic Benefits ◽

Production Costs ◽

Broiler Chicks ◽

Necrotic Enteritis ◽

Feed Conversion ◽

Intestinal Lesion

Necrotic enteritis (NE) caused by Clostridium perfringens (C. perfringens) results in impaired bird growth performance and increased production costs. Nanotechnology application in the poultry industry to control NE outbreaks is still not completely clarified. Therefore, the efficacy of dietary garlic nano-hydrogel (G-NHG) on broilers growth performance, intestinal integrity, economic returns and its potency to alleviate C. perfringens levels using NE challenge model were addressed. A total of 1200 male broiler chicks (Ross 308) were assigned into six groups; four supplemented with 100, 200, 300 or 400 mg of G-NHG/kg diet and co-challenged with C. perfringens at 21, 22 and 23 d of age and two control groups fed basal diet with or without C. perfringens challenge. Over the total growing period, the 400 mg/kg G-NHG group had the most improved body weight gain and feed conversion efficiency regardless of challenge. Parallel with these results, the mRNA expression of genes encoding digestive enzymes (alpha 2A amylase (AMY2A), pancreatic lipase (PNLIP) and cholecystokinin (CCK)) and intestinal barriers (junctional adhesion molecule-2 (JAM-2), occludin and mucin-2 (Muc-2)) were increased in groups fed G-NHG at higher levels to be nearly similar to those in the unchallenged group. At 14 d post challenge, real-time PCR results revealed that inclusion of G-NHG led to a dose-dependently decrease in the C. perfringens population, thereby decreasing the birds’ intestinal lesion score and mortality rates. Using 400 mg/kg of G-NHG remarkably ameliorated the adverse effects of NE caused by C. perfringens challenge, which contributed to better growth performance of challenged birds with rational economic benefits.

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Growth Performance, Serum Biochemical Indices, Duodenal Histomorphology, and Cecal Microbiota of Broiler Chickens Fed on Diets Supplemented with Cinnamon Bark Powder at Prestarter and Starter Phases

Animals ◽

10.3390/ani11010094 ◽

2021 ◽

Vol 11 (1) ◽

pp. 94

Author(s):

Mohammed M. Qaid ◽

Saud I. Al-Mufarrej ◽

Mahmoud M. Azzam ◽

Maged A. Al-Garadi ◽

Hani H. Albaadani ◽

...

Keyword(s):

Body Weight ◽

Broiler Chickens ◽

Body Weight Gain ◽

Basal Diet ◽

Positive Control ◽

Negative Control ◽

Broiler Chicks ◽

Feed Conversion ◽

Serum Globulin ◽

Maximum Improvement

Ross 308 broiler chicks (n = 240) aged 1 day were assigned to five groups for eight replicates (six chicks for each) (3♂ and 3♀). Basal dietary groups were supplemented by 2000, 4000, and 6000 mg/kg cinnamon (CN) for 21 days. Basal diet alone was used as a negative control, and basal antibiotic diet (Colimox) was used as a positive control. At 10, 14, and 21 days of age, chicks that received 2000 mg CN and Colimox had a higher body weight, resulting in an increase in body weight gain. CN also resulted in the maximum improvement in the feed conversion ratio and feed efficiency over 1–21 days at the level of 2000 mg/kg. At days 10, the maximum relative breast weight was 2000 mg/kg of CN. Mean serum albumin concentrations, duodenal villus height, and goblet cell density increased (p < 0.05) by 2000 mg/kg of CN, and mean serum globulin and total protein concentrations and crypt depth increased (p < 0.05) by 6000 mg/kg of CN compared with control. Increased cecal Escherichia coli number was CN dose-dependent. In conclusion, dietary inclusion of 2000 mg/kg CN can be applied as an alternative to in-feed antibiotics for broiler starter diet.

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