Asymmetric dimethylarginine, homocysteine and renal function – is there a relation?

2005 ◽  
Vol 43 (10) ◽  
Author(s):  
Romana Široká ◽  
Ladislav Trefil ◽  
Daniel Rajdl ◽  
Jaroslav Racek ◽  
Hana Rusňáková ◽  
...  
Keyword(s):  
Blood Donors ◽  
Asymmetric Dimethylarginine ◽  
Linear Regression Analysis ◽  
Vascular Wall ◽  
Multiple Linear Regression Analysis ◽  
Homocysteine Concentration ◽  
Group A ◽  
Edta Plasma ◽  
Positive Correlations ◽  
Group B

AbstractThe adverse effect of hyperhomocysteinemia on the vascular wall can be partially explained by increasing plasma concentration of asymmetric dimethylarginine (ADMA), a potent inhibitor of nitric oxide synthase. The aim of the study was to compare ADMA and homocysteine levels in three groups of subjects: blood donors with normal homocysteine concentration (group A), patients with hyperhomocysteinemia and normal kidney function (group B) and hemodialysis patients who are known to be hyperhomocysteinemic (group C). Concentrations of homocysteine (enzymatic method), ADMA (enzyme-linked immunoassay) and creatinine (Jaffe method) in EDTA plasma were measured. Plasma ADMA levels were significantly higher in both groups with hyperhomocysteinemia (1.60±0.56μmol/L in group B, 1.81±0.57μmol/L in group C) when compared with those in blood donors (0.82±0.29μmol/L, p<0.001 in both cases). Significant positive correlations were found between concentrations of ADMA and homocysteine (r=0.42, p<0.0001), ADMA and creatinine (r=0.39 p<0.001), homocysteine and creatinine (r=0.69, p<0.0001), age and homocysteine (r=0.47, p<0.001), age and ADMA (r=0.57, p<0.001) and age and creatinine (r=0.37, p<0.001). Increased ADMA concentrations in hyperhomocysteinemic patients were confirmed, but multiple linear regression analysis showed that this significant correlation is only apparent due the dependence of both parameters on age.

scholarly journals The effects of backward, upward, rightward pressure maneuver for intubation using the OptiscopeTM: a retrospective study

2021 ◽  
Author(s):  
Sei-hoon Oh ◽  
Sang-kwon Heo ◽  
Seung-Uk Cheon ◽  
Seung-Ah Ryu
Keyword(s):  
Linear Regression Analysis ◽  
Multiple Linear Regression Analysis ◽  
Intubation Time ◽  
Dental Injury ◽  
Obstructive Sleep ◽  
History Of ◽  
Group A ◽  
Neck Rotation ◽  
Interincisor Distance ◽  
Group B

Background: The OptiscopeTM and the backward, upward, rightward pressure (BURP) maneuver are widely used in clinical practice because the BURP maneuver facilitates intubation by improving visualization of the larynx. However, the effect of the BURP maneuver is unclear when using the OptiscopeTM. Therefore, we retrospectively investigated the effect of the BURP maneuver on intubation using the OptiscopeTM.Methods: Sixty-eight patients intubated with the OptiscopeTM were enrolled. We used the BURP maneuver in Group A (n = 33) and the conventional maneuver (which does not use the BURP maneuver) in Group B (n = 35). BURP application status was a binary variable representing whether the BURP maneuver was used during the intubation. A multiple linear regression analysis was performed to assess the effects of the BURP application status on intubation time controlling for body mass index, preoperative dental injury status, obstructive sleep apnea history, thyromental distance, sternomental distance (SMD), interincisor distance, history of neck rotation restriction, and Mallampati classification.Results: There was no difference in the intubation time between the two groups. According to the regression model (R2 = 0.308, P = 0.007), the BURP maneuver (Group A) decreased the intubation time by 6.089 seconds (95% confidence interval 1.303–10.875, P = 0.014) compared to Group B.Conclusion: The BURP maneuver reduced intubation time when using the OptiscopeTM.

scholarly journals Factors Affecting Thyroid Hormone Changes Over 1 Month After Birth in Preterm Newborns

2021 ◽  
Vol 5 (Supplement_1) ◽  
pp. A714-A714
Author(s):  
Seulki Kim ◽  
Sung Eon Kim ◽  
Na Yeong Lee ◽  
Moon Bae Ahn ◽  
Shin Hee Kim ◽  
...  
Keyword(s):  
Thyroid Hormone ◽  
Linear Regression Analysis ◽  
Reference Value ◽  
Multiple Linear Regression Analysis ◽  
Thyroid Stimulating Hormone ◽  
Normal Brain ◽  
Factors Affecting ◽  
Group A ◽  
Group B ◽  
Preterm Newborns

Abstract Purpose: To analyze factors affecting thyroid hormone changes over 1 month after birth in preterm newborns. Methods: Thyroid hormones, including free thyroxine (fT4) and thyroid stimulating hormone (TSH) of 216 preterm newborns (mean gestational age [GA] 33.6±2.6 weeks), were retrospectively reviewed at the 1st and 4th weeks after birth. Preterm newborns were classified into three groups according to the GA (&lt; 28 weeks, group A; 28-32 weeks, group B; 32-36 weeks, group C). The association between thyroid hormone changes and clinical factors was analyzed. Results: Preterm newborns with older GA had higher concentrations of fT4 (group A, 0.9±0.2 ng/dL; group B, 1.2±0.2 ng/dL; group C, 1.5±0.3 ng/dL) and TSH (group A, 2.7±3.2 mIU/L; group B, 4.5±3.3 mIU/L; group C, 6.0±3.8 mIU/L), both at the 1st week after birth. fT4 and TSH at the 1st week after birth were positively correlated with the GA (r=0.52, P=0.0001; r=0.30, P=0.0001) and Apgar score at 5 minutes (r=0.31, P=0.0001; r=0.28, P=0.0001). TSH concentration at the 1st week after birth was significantly lower in the abnormal brain sonogram group than in the normal brain sonogram group (4.4±3.7 vs 6.1±3.4 mIU/L, P=0.001); there were no differences at the 4th week after birth between these groups (4.2±2.6 vs 3.4±2.4 mIU/L, P=0.485). Multiple linear regression analysis revealed that GA was positively correlated with fT4 and TSH at the 1st week, and fT4 at 4th week after birth (B=0.08, SE=0.12, P=0.0001; B=0.34, SE=0.15, P=0.029; B=0.02, SE=0.01, P=0.013). Conclusion: Thyroid hormone levels in preterm newborns are mostly within the normal reference value of full-term newborns within 4 weeks of life, and are mainly affected by GA and brain ultrasound abnormalities.

scholarly journals Blood asymmetric dimethylarginine and nitrite/nitrate concentrations in short-stature children born small for gestational age with and without growth hormone therapy

2017 ◽  
Vol 46 (2) ◽  
pp. 761-772 ◽  
Author(s):  
Hironori Nagasaka ◽  
Ichiro Morioka ◽  
Mayuko Takuwa ◽  
Mariko Nakacho ◽  
Mayumi Yoshida ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Growth Hormone ◽  
Asymmetric Dimethylarginine ◽  
Therapy Group ◽  
Adma Level ◽  
Gh Therapy ◽  
Normal Children ◽  
Group A ◽  
Group B ◽  
Nitrite Nitrate

Objective To investigate the basal amino acid metabolism and impact of growth hormone (GH) therapy in short-stature children born small for gestational age (short SGA children). Methods In this age-matched case-control study, the basal blood levels of amino acids, asymmetric dimethylarginine (ADMA), and nitrite/nitrate (NOx) were compared between 24 short SGA children and 25 age-matched normal children. Changes in these parameters were assessed for 12 months in 12 short SGA children initiating GH therapy (Group A) and 12 age-matched short SGA children without GH therapy (Group B). Results The arginine levels were significantly lower in the short SGA than in normal children. The ADMA levels were significantly higher and NOx levels were significantly lower in the short SGA than normal children. In Group A, the ADMA level was significantly lower and NOx level was significantly higher at 6 months than at baseline. At 12 months, the ADMA level in Group A began to increase, but the NOx level remained the same. Group B showed no significant changes. Conclusions This study is the first to show that ADMA is promoted and nitric oxide is suppressed in short SGA children and that GH therapy affects the production of ADMA and nitric oxide.

scholarly journals ANALISIS FAKTOR-FAKTOR YANG MEMPENGARUHI NIAT UNTUK BERDONOR DARAH SECARA REGULER DI UTD PMI KABUPATEN BANTUL YOGYAKARTA

2020 ◽  
Vol 8 (2) ◽  
pp. 100-107
Author(s):  
Francisca Romana Sri Supadmi ◽  
Nur’Aini Purnamaningsih ◽  
Rijantono Franciscus Maria
Keyword(s):  
Regression Analysis ◽  
Theory Of Planned Behavior ◽  
Planned Behavior ◽  
Significant Influence ◽  
Blood Donors ◽  
Behavioral Control ◽  
Linear Regression Analysis ◽  
Multiple Linear Regression Analysis ◽  
Subjective Norms ◽  
Sampling Techniques

Background: Blood supplies estimated to be around 2% of the total population1. However, only 1.88 million bags of blood can be met or 40% of the total needs, so there is a 60% lack of blood from the minimum requirement2. Objective: The purpose of this study was to analyze the factors that influence the intention to donate blood regularly at UTD PMI Bantul Regency Yogyakarta using the Theory of Planned Behavior (TPB) approach. Methods: This research was conducted at UTD PMI Bantul Regency, Yogyakarta in September-December 2018. The number of respondents was 35 voluntary blood donors, with sampling techniques using accidental sampling. Results: The results of multiple linear regression analysis show that attitudes, subjective norms, and behavioral control have a significant effect on the intention to donate. While the results of the regression analysis partially, based on a significant level of value, attitudes have a significant influence on one's intention in donating, while subjective norms and behavioral control do not significantly influence one's intentions in donating. Conclusion: The attitude had the most significant influence on the intention to donate regularly on UTD PMI Bantul Regency Yogyakarta using the Theory of Planned Behavior (TPB) approach.

Abstract T P337: Asymmetric Dimethylarginine Is Related to the Predicted Stroke Risk in Middle-aged Japanese Men

Stroke ◽  
2014 ◽  
Vol 45 (suppl_1) ◽  
Author(s):  
yasuhiro nishiyama ◽  
Masayuki Ueda ◽  
Yasuo Katayama ◽  
Toshiaki Otsuka
Keyword(s):  
Asymmetric Dimethylarginine ◽  
Stroke Risk ◽  
Smoking Status ◽  
Linear Regression Analysis ◽  
Multiple Linear Regression Analysis ◽  
Cerebrovascular Diseases ◽  
Adma Level ◽  
Middle Aged ◽  
Cross Sectional ◽  
Japanese Men

Background: Asymmetric dimethylarginine (ADMA) has recently been investigated as a risk marker for cardio- and cerebrovascular diseases. However, it remains unclear whether ADMA levels are related to the risk of stroke in the general Japanese population. Methods and Results: In this cross-sectional study, we examined 769 Japanese men (mean, 47 ± 5 years) who underwent health examinations. Each patient’s ADMA level and various vascular risk factors were assessed, and the predicted 10-year risk of stroke was calculated using the point-based prediction model for incident total stroke for Japanese from the Japan Public Health Center Study. In a multiple linear regression analysis, age, body mass index, estimated glomerular filtration rate, and current smoking status were significant independent determinants of the ADMA level. A significant odds ratio (OR) for high predicted stroke risk (10-year risk ≥ 5%) was noted in the highest quartile of ADMA level (OR, 2.47; 95% CI, 1.00-6.07) as compared to the lowest quartile, even after adjusting for potential confounding factors. A significant OR for high predicted stroke risk was also found for each standard-deviation increment in ADMA level (adjusted OR, 1.46; 95% CI, 1.10-1.92). Conclusions: An elevated ADMA level was significantly associated with an increase in predicted stroke risk, suggesting that the measurement of ADMA level may be useful for identifying middle-aged Japanese men at high risk for stroke.

scholarly journals Association of Viral Hepatitis with ABO Blood Group in apparently Normal Iraqi Blood Donors

2020 ◽  
Vol 10 (03) ◽  
pp. 421-425
Author(s):  
Suad Azeez Hassan ◽  
Suhair Hassan Alkutbi ◽  
Eman S. Nassir ◽  
Haider Hassan Lilo
Keyword(s):  
Hepatitis B ◽  
Blood Group ◽  
Blood Donors ◽  
Blood Groups ◽  
Public Health Issue ◽  
Blood Group A ◽  
B Virus ◽  
Group A ◽  
Group B ◽  
Apparently Healthy

Hepatitis B virus (HBV) and hepatitis C virus (HCV) infections are counted as a public health issue worldwide. The virus is transmitted to infect others through blood and blood products. Appointed blood groups and Rh-positive are more prone to the transmission of the infection by blood transfusion. The aim of this study is to find out the frequency of hepatitis B and C in apparently healthy blood donors and whether there is an association between ABO and Rh blood groups. ABO blood groups and their relationship with HBV and HCV infections were studied in 87,124 blood donors of both genders. Out of these donors, 353 individuals were found to be infected with HBV and HCV with a ratio of 1:250. The study was conducted between January to June 2018, which was presented to the Central Blood Bank in Baghdad and Al-Razi Medical Centre. It found that most hepatitis B and C blood donors were blood group O with a prevalence of 33.7 and 45.5%, respectively, while the results for those with blood group A showed 28.5 and 22.7% HBV and HCV infections, respectively. On the other hand, the incidence of HBV in individuals with blood group B was 29.8%, and HCV was 23.8%. AB blood group donors demonstrated the least incidence at 7.9% for both HBV and HCV. In conclusion, it has been found that there is a significant association between blood groups and Rh factor with hepatitis B and C infections.

scholarly journals Distribution of ABO, Rhesus D and Subgroups of ABO among Blood Donors in Sokoto, North Western Nigeria

2020 ◽  
pp. 6-13
Author(s):  
A. B. Ibrahim ◽  
H. Attahiru ◽  
O. Erhabor ◽  
P. F. Udomah ◽  
A. Yakubu ◽  
...  
Keyword(s):  
Blood Group ◽  
Blood Donors ◽  
Blood Groups ◽  
Blood Group A ◽  
Significant Difference ◽  
Tile Method ◽  
Group A ◽  
The Common ◽  
Group B ◽  
North Western

ABO, Rhesus D and subgroups of ABO are highly immunogenic and are the common cause of antibody production in mismatched blood transfusions, haemolytic transfusion reaction and maternal alloimmunization. The aim of this study was to determine the occurrence of ABO, Rh D and subgroups of ABO among blood donors attending Specialist Hospital Sokoto, Nigeria. ABO, Rhesus D and subgroups of ABO antigen status of 176 blood donors with mean age of 30.44 ± 8.210 years attending Specialist Hospital Sokoto were determined using tile method for ABO and Rh D and conventional tube method for anti- A1, anti- H reagents for ABO subgroups respectively. Among the 176 subjects tested, blood group O+ was the most frequent group with 93 (52.8%), 39 (22.2%) were blood group B+, 37(21.0%) were blood group A+, 5 (2.8%) were blood group AB+, 2 (1.1%) were blood group O-. No data was obtained for A-, B- and AB- blood groups.  Out of 37 A blood groups obtained, 31 (83.8%) had A1 antigens and 6 (16.2%) had A2 antigens. Out of the 5 AB blood groups, all had A1B antigens. The study also shows that there was statistically significant difference between blood group A and ethnic groups (Hausa, Fulani and Yoruba) (p<0.05). Blood group O was found to be the most frequent followed by B, A and AB except among Hausa which revealed a pattern of O> A> B> AB. ABO, subgroups shows majority had A1 followed by A2 and A1B respectively.

scholarly journals Histology and Immunohistochemistry of Radial Arteries Are Suggestive of an Interaction between Calcification and Early Atherosclerotic Lesions in Chronic Kidney Disease

Medicina ◽  
2021 ◽  
Vol 57 (11) ◽  
pp. 1156
Author(s):  
Aikaterini Lysitska ◽  
Nikiforos Galanis ◽  
Ioannis Skandalos ◽  
Christina Nikolaidou ◽  
Sophia Briza ◽  
...  
Keyword(s):  
Chronic Kidney Disease ◽  
Kidney Disease ◽  
Vascular Calcification ◽  
Morphometric Analysis ◽  
Intima Media Thickness ◽  
Inflammatory Cells ◽  
Vascular Wall ◽  
Control Group ◽  
Group A ◽  
Group B

Background and Objectives: recent studies suggest an implication of immune mechanisms in atherosclerotic disease. In this paper, the interaction between inflammation, calcification, and atherosclerosis on the vessel walls of patients with chronic kidney disease (CKD) is described and evaluated. Materials and Methods: patients with stage V CKD, either on pre-dialysis (group A) or on hemodialysis (HD) for at least 2 years (group B), in whom a radiocephalic arteriovenous fistula (RCAVF) was created, were included in the study. The control group included healthy volunteers who received radial artery surgery after an accident. The expressions of inflammatory cells, myofibroblasts, and vascular calcification regulators on the vascular wall were estimated, and, moreover, morphometric analysis was performed. Results: the expressions of CD68(+) cells, matrix carboxyglutamic acid proteins (MGPs), the receptor activator of nuclear factor-kB (RANK) and RANK ligand (RANKL), and osteoprotegerin (OPG), were significantly increased in CKD patients compared to the controls p = 0.02; p = 0.006; p = 0.01; and p = 0.006, respectively. In morphometric analysis, the I/M and L/I ratios had significant differences between CKD patients and the controls 0.3534 ± 0.20 vs. 0.1520 ± 0.865, p = 0.003, and 2.1709 ± 1.568 vs. 4.9958 ± 3.2975, p = 0.03, respectively. The independent variables correlated with the degree of vascular calcification were the intensity of CD34(+), aSMA(+) cells, and OPG, R2 = 0.76, p < 0.0001, and, with intima-media thickness (IMT), the severity of RANKL expression R2 = 0.3, p < 0.0001. Conclusion: atherosclerosis and vascular calcification in CKD seem to be strongly regulated by an immunological and inflammatory activation on the vascular wall.

Repeatability of ultrasound examination of the optic nerve sheath diameter in the adult cat: comparison between healthy cats and cats suffering from presumed intracranial hypertension

2020 ◽  
Vol 22 (10) ◽  
pp. 959-965 ◽  
Author(s):  
Maria Antonietta Evangelisti ◽  
Giovanni Carta ◽  
Giovanni P Burrai ◽  
Maria Luisa Pinna Parpaglia ◽  
Francesca Cubeddu ◽  
...  
Keyword(s):  
Optic Nerve ◽  
Intracranial Hypertension ◽  
Linear Regression Analysis ◽  
Optic Nerve Sheath Diameter ◽  
Optic Nerve Sheath ◽  
Nerve Sheath ◽  
Group A ◽  
The Mean ◽  
Group B ◽  
The Right

Objectives The objectives of this study were to test: (1) the repeatability of ultrasonographic examination of the optic nerve sheath diameter (ONSD) in the cat; (2) the association between the ONSD and age, sex and body weight in healthy cats; and (3) the difference in the ONSD between healthy cats and those suffering from presumed intracranial hypertension (ICH). Methods This study had a prospective, blinded, observational cross-sectional study design. Two groups of animals were considered: healthy cats (group A) and cats with a diagnosis of presumed ICH (group B). The ONSD was evaluated, measured and compared between the two groups via an ultrasonographic transpalpebral approach. Repeatability of the procedure was evaluated through the intraclass correlation coefficient (ICC). Data were statistically compared using the Student’s t-test and linear regression analysis. Results A strong inter- and intraobserver ICC indicating good repeatability was observed. The interobserver ICC was 0.965 ( P  <0.05) for the right eye and 0.956 ( P  <0.05) for the left eye. The intraobserver ICC was 0.988 ( P  <0.05) and 0.984 ( P  <0.05) for the right and left eyes, respectively. In healthy cats the mean ± SD ONSD was 1.23 ± 0.11 mm (range 1–1.47 mm) and 1.23 ± 0.10 (range 1–1.4 mm) for right and left eyes, respectively. The ONSD was not related to sex or weight; a weak relationship was observed with age. In group B, the mean ONSD was 1.68 ± 0.13 mm (range 1.5–1.9 mm) and 1.61 ± 0.15 mm (range 1.4–1.9 mm) for the right and left eyes, respectively. In group B, the ONSD was statistically significantly larger than in group A, the healthy cats ( P  <0.001). Conclusions and relevance The transpalpebral ultrasonographic technique is a non-invasive, feasible and reproducible method to measure ONSD both in healthy cats and in cats suffering from suspected ICH.

High Frequency of Unusual ABO Alleles in Patients with Leukemia.

Blood ◽  
2004 ◽  
Vol 104 (11) ◽  
pp. 2712-2712
Author(s):  
Marcia C.Z. Novaretti ◽  
Madalena M.N.S. Pares ◽  
Alexandre E. Domingues ◽  
Pedro E. Dorlhiac-llacer ◽  
Dalton A.F. Chamone
Keyword(s):  
Blood Group ◽  
Blood Donors ◽  
Catalytic Domain ◽  
Test Technique ◽  
Single Nucleotide ◽  
Pcr Screening ◽  
Specific Pcr ◽  
Group A ◽  
Group B

Abstract Background: Mutations critical for ABO group phenotypes which encode the catalytic domain of ABO glycosyltransferases have been studied in healthy blood donors. Weakening of A and B transferases have been reported in patients with leukemia, particularly those in which the myeloid lineage is involved. The purpose of this study was to use sequence specific PCR (PCR-SSP) to perform ABO genotyping of leukemic patients. Material and Methods: A total of 108 unrelated leukemic patients were initially classified serologically using tube test technique and gel test for ABO typing and subgroup detection. Genomic DNA was prepared from peripheral blood cells. All samples were then screened by 32 different PCR-SSP, each specific for a single nucleotide variation to screen 16 known polymorfic sites of exons 6 and 7 (modified from Seltsam, Transfusion 2003,43(4):428–39. Results: The results of PCR screening were conclusive and consistent with the ABO phenotypes in 71 patients (65.07%). Common ABO alleles were found in only 15 (13.8%) of patients studied. Unusual ABO alleles were found in 26 of 28 blood group O patients (ABO*O05, ABO*O21, ABO*O30, ABO*O33, ABO*O35, ABO*O36, ABO*O43 and ABO*O45), in 20 of 43 blood group A patients (ABO*A103, ABO*A104, ABO*A106, ABO*A202, ABO*A203), in 8 of 10 blood group B patients (ABO*B104, ABO*B105) and in 2 of 4 AB blood group patients (ABO*Bx01, ABO*B103). In 37 patients (34.2%), PCR screening revealed allele combinations that were incompatible with known ABO allele combinations or subgroups predicted by serologic analysis. Conclusion: This is the first report of using PCR-SSP for ABO genotyping of leukemic patients. The pathophysiologic role of unusual ABO allele combinations in patients with leukemia warrants further investigation.

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