scholarly journals Novel test procedure for testing antimicrobially active materials

2018 ◽  
Vol 4 (1) ◽  
pp. 229-231
Author(s):  
Alexander Stich ◽  
Sebastian Buhl ◽  
Clemens Bulitta
Keyword(s):  
Antimicrobial Activity ◽  
Standard Method ◽  
Test Procedure ◽  
Practical Application ◽  
Active Materials ◽  
Antimicrobial Coatings ◽  
Artificial Structure ◽  
Antimicrobial Test ◽  
Cleaning Procedures

AbstractAntimicrobial coatings are typically tested for their activity and effectiveness based on an artificial procedure, the standard JIS Z 2801 (or ISO 22196). The test samples are contaminated with a predetermined concentration of bacteria and covered with a sterile film. Due to the artificial structure of this process, however, there is always the question of the validity in the practical application of these surfaces and how comparable this germ-reducing effect is to currently common disinfection measures. Therefore, we developed a more realistic test procedure in our laboratory. The test samples are inoculated and incubated at standard room conditions. To compare the antimicrobial activity to standard cleaning procedures also disinfection and wipe controls were performed. The results show that the antimicrobial test patterns we use have a strong efficacy. This could be shown reproducibly both with the standard method JIS Z 2801 as well as with our new test procedure.

scholarly journals Edible Films from Carrageenan/Orange Essential Oil/Trehalose—Structure, Optical Properties, and Antimicrobial Activity

Polymers ◽  
2021 ◽  
Vol 13 (3) ◽  
pp. 332
Author(s):  
Jancikova Simona ◽  
Dordevic Dani ◽  
Sedlacek Petr ◽  
Nejezchlebova Marcela ◽  
Treml Jakub ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Essential Oil ◽  
Antibacterial Properties ◽  
Edible Films ◽  
Attenuated Total Reflectance ◽  
Practical Application ◽  
Film Properties ◽  
Gram Positive Bacteria ◽  
Edible Packaging ◽  
Orange Essential Oil

The research aim was to use orange essential oil and trehalose in a carrageenan matrix to form edible packaging. The edible packaging experimentally produced by casting from an aqueous solution were evaluated by the following analysis: UV-Vis spectrum, transparency value, transmittance, attenuated total reflectance Fourier-Transform spectroscopy (FTIR), scanning electron microscopy (SEM) and antimicrobial activity. The obtained results showed that the combination of orange essential oil with trehalose decreases the transmittance value in the UV and Vis regions (up to 0.14% ± 0.02% at 356 nm), meaning that produced films can act as a UV protector. Most produced films in the research were resistant to Gram-positive bacteria (Staphylococcus aureus subsp. aureus), though most films did not show antibacterial properties against Gram-negative bacteria and yeasts. FTIR and SEM confirmed that both the amount of carrageenan used and the combination with orange essential oil influenced the compatibility of trehalose with the film matrix. The research showed how different combinations of trehalose, orange essential oils and carrageenan can affect edible film properties. These changes represent important information for further research and the possible practical application of these edible matrices.

Development of linseed oil–TiO2 green nanocomposites as antimicrobial coatings

2015 ◽  
Vol 3 (21) ◽  
pp. 4458-4471 ◽  
Author(s):  
Ana M. Díez-Pascual ◽  
Angel L. Díez-Vicente
Keyword(s):  
Antimicrobial Activity ◽  
Linseed Oil ◽  
Nanocomposite Coatings ◽  
Public Places ◽  
Uv Curable ◽  
Antimicrobial Coatings ◽  
Microbial Proliferation ◽  
Epoxidized Linseed Oil

UV-curable acrylated epoxidized linseed oil–TiO2 nanocomposite coatings with antimicrobial activity were developed to prevent microbial proliferation in public places like hospitals.

Chemical composition, antioxidant and antimicrobial activities of essential oil of Thymus algeriensis wild-growing in Libya

2013 ◽  
Vol 8 (5) ◽  
pp. 504-511 ◽  
Author(s):  
Abdulhmid Giweli ◽  
Ana Džamić ◽  
Marina Soković ◽  
Mihailo Ristić ◽  
Petar Marin
Keyword(s):  
Antimicrobial Activity ◽  
Antioxidant Activity ◽  
Chemical Composition ◽  
Essential Oil ◽  
Antimicrobial Activities ◽  
Practical Application ◽  
Dpph Assay ◽  
High Antimicrobial Activity ◽  
Thymus Algeriensis ◽  
Antioxidant And Antimicrobial Activities

AbstractThe composition of essential oil isolated from Thymus algeriensis growing wild in Libya was analyzed by GC and GC-MS. The essential oil was characterized with thymol (38.50%) as the major component. The oil was screened for antioxidant activity using DPPH assay, and compared to thymol and carvacrol. Antioxidant activity was high, with the IC50 of 0.299 mg/ml, compared to 0.403 and 0.105 mg/ml for thymol and carvacrol, and 0.0717 mg/ml for BHA. In addition, antimicrobial activity was tested against eight bacteria and eight fungi. T. algeriensis oil showed inhibitory activity against tested bacteria at 0.001–0.05 mg/ml, while bactericidal activity (MBC) was achieved at 0.0025–0.05 mg/ml. For antifungal activity MICs ranged 0.0005–0.025 mg/ml and MFC 0.001–0.05 mg/ml. High antimicrobial activity against the fungi in particular suggests that the essential oil of Thymus algeriensis could have a useful practical application.

scholarly journals Ecofriendly Antimicrobial Dyeing for Cotton Fabric Using Natural Extract of Marigold

2021 ◽  
Vol 12 (2) ◽  
pp. 957-962
Author(s):  
Dr. Suneeta, Et. al.
Keyword(s):  
Antimicrobial Activity ◽  
Cotton Fabric ◽  
Natural Resource ◽  
Textile Industry ◽  
Cotton Fabrics ◽  
Tagetes Erecta ◽  
Light Fastness ◽  
Antimicrobial Coatings ◽  
Natural Extract ◽  
Prospective Application

The main drawback with microorganism's development on fabrics is associated with the maintenance of hygiene and fabric deterioration. In this present investigation a trial has been made to produce an attractive and vibrant textile with eco-friendly antimicrobial dyeing by marigold, a natural resource. Tagetes erecta universally recognized as Marigold is a remarkable source of carotenoids and lutein, grown as a lawn plant. Now a day, lutein is exploding into an indubitably predominant active fitting, employed as an element of the Drugs, Nutrients and Textile Industry. The prospective application of marigold as a characteristic of antimicrobial activity has not been accustomed fully. It is due to the lack of knowledge about its ability and resemblance in material manner. In this work, we have concentrated on the analysis, which was led to deliberate the use of distillate isolate of marigold as an antimicrobial. The antimicrobial competence of the extract was evaluated by coloring on cotton textiles. Testing of the dye ability, its wash quickness and light fastness was done. Educations have validated that antimicrobial coatings have not affected by laundering and shown enhanced colouring effect comprising of fastness to washing, perspiration and rubbing than conservatively dyed textiles. It revealed that the essence of Marigold flower is habitually employed for cotton fabrics as an antimicrobial finish. The samples show very encouraging ends up in terms of antimicrobial resistivity.

scholarly journals PERBANDINGAN KADAR FENOLIK DAN AKTIVITAS ANTIMIKROBA RIMPANG JERINGAU (Acorus calamus) SEGAR DAN TERFERMENTASI

2020 ◽  
Vol 3 (1) ◽  
pp. 16
Author(s):  
Fitri Hardiansi ◽  
Dwi Afriliana ◽  
Anita Munteira ◽  
Ernanin Dyah Wijayanti
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Candida Albicans ◽  
Antimicrobial Agent ◽  
Phenolic Content ◽  
Plant Cells ◽  
Acorus Calamus ◽  
Antimicrobial Test

ABSTRACTCalamus rhizome contain phenolic as antimicrobial agent. Fermentation can increase thereleasing of phenolic content from plant cells. The aim of this research was to observe thecomparison of phenolic content and antimicrobial activity between fresh and fermentedcalamus rhizome extract. Phenolic content was determined by using Folin-Ciocalteu method,and antimicrobial test by agar well diffusion. Phenolic content of fresh and fermentedcalamus rhizome extract respectively are 97,272±0,525 and 223,553±3,542 mgGAE/gram.Inhibiton zone against Staphylococcus aureus are 4,04±0,05 and 8,24±0,58 mm, whileagainst Candida albicans are 14,90±0,57 and 18,16±1,47 mm. Fermentation increase phenoliccontent and antimicrobial activity of calamus rhizome.Key words: antimicrobial, calamus rhozome, fermentation, phenolicABSTRAKRimpang jeringau memiliki kandungan senyawa fenolik sebagai antimikroba. Fermentasidapat meningkatkan pelepasan senyawa fenolik pada sel tanaman. Penelitian ini bertujuanuntuk mengetahui perbandingan kadar fenolik dan aktivitas antimikoba ekstrak rimpangjeringau segar dan terfermentasi. Penetapan kadar fenolik dilakukan dengan metode Folin-Ciocalteu dan uji antimikroba dengan metode difusi sumuran. Kadar fenolik ekstrak jeringausegar dan fermentasi secara berurutan sebesar 97,272±0,525 dan 223,553±3,542mgGAE/gram. Daya hambat rimpang jeringau segar dan terfermentasi terhadapStaphylococcus aureus sebesar 4,04±0,05 dan 8,24±0,58 mm, sedangkan terhadap Candidaalbicans sebesar 14,90±0,57 dan 18,16±1,47 mm. Fermentasi dapat meningkatkan kadarfenolik dan aktivitas antimikroba rimpang jeringau.Kata kunci: antimikroba, fenolik, fermentasi, rimpang jeringau

scholarly journals Studies on Target-specificity and Biological Activity of Streptococcus Serum Antibody and Sulfate Amikacin Conjugates

2019 ◽  
pp. 1-10
Author(s):  
Lingxiao Yi ◽  
Xiang Li ◽  
Wei Dong
Keyword(s):  
Antimicrobial Activity ◽  
Biological Activity ◽  
Acute Toxicity ◽  
Specific Binding ◽  
Serum Antibody ◽  
Target Specificity ◽  
Practical Application ◽  
Response Sensitivity ◽  
Polyethylene Glycol 6000 ◽  
And Staphylococcus Aureus

To investigate the target-specificity and biological activity of Streptococcus serum antibody and sulfate amikacin conjugates. The recent used polyethylene glycol 6000 (PEG6000) as the coupling agent to produce Coupled complexes of Streptococcus serum antibody and sulfate amikacin. Then, analyzed the antibody being in conjugates specificity which against Streptococcus, and the antibody being in conjugates immunogenicity. Besides, we also detected the acute toxicity, antimicrobial activity and bioavailability of sulfate amikacin being in conjugates. As a result, the antibody specific binding to Streptococcus, instead of Escherichia coil, Pasteurella and Staphylococcus aureus. Biological activity results showed that coupling decreased Streptococcus serum antibody immunogenicity, increased Streptococcus serum antibody response sensitivity. Simultaneously, the results indicated that coupling reduced the acute toxicity of sulfate amikacin, improved sulfate amikacin bioavailability and antimicrobial activity of sulfate amikacin. The combination effect on the antibacterial activity of drug and the biological activity of serum antibody is helpful for the practical application of targeted drugs.

A Review on Antimicrobial Coatings for Biomaterial Implants and Medical Devices

2020 ◽  
Vol 16 (6) ◽  
pp. 789-809
Author(s):  
Long Chen ◽  
Xinyu Song ◽  
Fei Xing ◽  
Yanan Wang ◽  
Yuanzheng Wang ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Medical Devices ◽  
Modern Medicine ◽  
Implant Surface ◽  
Antimicrobial Coatings ◽  
Antimicrobial Coating ◽  
Compound Ii ◽  
Topical Antimicrobial

Biomaterial implants and medical devices have been utilized extensively in medical treatment with the development of modern medicine, especially in orthopaedics and stomatology. Along with their applications, biomaterial-associated infections (BAIs) have grown to be one of the main postoperative complications. Antimicrobial coating strategies have been reported to effectively inhibit bacterial adhesion and proliferation on implant surface, extending their lifespan. In this review, the most topical antimicrobial coating designs have been chosen from literature studies. Their antimicrobial mechanisms and antimicrobial activity assessments in literature studies have been presented and compared. Based on their active ingredients, antimicrobial coatings are categories into (i) inorganic agents, including Ag, Cu, ZnO, MoS2 and nitride compound; (ii) organic agents including antibiotic, antimicrobial peptides, polymer, essential oils etc. The review has provided various and detailed options of antimicrobial coating designs for consulting according to their specific application. It is noted that the research of antimicrobial coatings is mostly in vitro and in vivo animal models study. It is thus in need for more preclinical or clinical studies, especially finding the direct connection between the utilization of antimicrobial coated implants and the reduction in BAIs incidence. Furthermore, future antimicrobial coating designs shall respect also biocompatibility, functionality, and durability apart from their antimicrobial activity.

scholarly journals Antimicrobial activity and acute and chronic toxicity of the essential oil of Lippia origanoides

2014 ◽  
Vol 34 (12) ◽  
pp. 1153-1161 ◽  
Author(s):  
Viviane A. Andrade ◽  
Anna C. Almeida ◽  
Dayane S. Souza ◽  
Keila G.F. Colen ◽  
Auricélio A. Macêdo ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Essential Oil ◽  
Biochemical Parameters ◽  
Chronic Toxicity ◽  
Safety Margin ◽  
Toxic Effects ◽  
Histopathological Changes ◽  
Practical Application ◽  
E Coli ◽  
Hematological And Biochemical Parameters

Currently, there is a growing interest in medicinal plants, because of an increased demand for alternate therapies. In this study, the antimicrobial activity and toxicity of the essential oil of Lippia origanoides (L. origanoides) were investigated. The essential oil of L. origanoides was extracted by steam-dragging distillation and its constituents were identified by chromatography coupled with mass spectrometry. Among the 15 compounds identified, the most abundant were carvacrol (29.00%), o-cymene (25.57%), and thymol methyl ether (11.50%). The essential oil was studied in antimicrobial assays to determine the MIC and MBC. The results indicated that a concentration of 120μL/mL of oil was sufficient to inhibit the growth of the following microorganisms: Escherichia coli (ATCC 25922), Staphylococcus aureus (ATCC 25923) and Salmonella cholerasuis (ATCC 10708). Acute and chronic toxic effects of orally administered oil were investigated in Wistar rats by using standard methods. Doses of 30, 60 and 120mg/kg of the essential oil did not induce significant changes in weight, behavior or hematological and biochemical parameters in the animals. There were no signs of any histopathological changes to the liver, kidneys or heart of the treated rats, suggesting that Lippia origanoides oil is non-toxic after oral administration in acute or chronic toxicity studies. The results obtained in this study show that the essential oil of L. origanoides has a high safety margin, with no detectable toxic effects in rats treated with doses to 120mg/kg. In addition, L. origanoides oil demonstrated potent antimicrobial activity against S. aureus, E. coli and S. cholerasuis. Based on these findings, this essential oil may have practical application as a veterinary antimicrobial.

scholarly journals Nanocellulose in Drug Delivery and Antimicrobially Active Materials

Polymers ◽  
2020 ◽  
Vol 12 (12) ◽  
pp. 2825
Author(s):  
Kaja Kupnik ◽  
Mateja Primožič ◽  
Vanja Kokol ◽  
Maja Leitgeb
Keyword(s):  
Drug Delivery ◽  
Antimicrobial Activity ◽  
Sustained Release ◽  
Drug Delivery Systems ◽  
Antimicrobial Agents ◽  
Metal Oxide Nanoparticles ◽  
Biological Properties ◽  
Oxide Nanoparticles ◽  
Active Materials ◽  
Natural Sources

In recent years, nanocellulose (NC) has also attracted a great deal of attention in drug delivery systems due to its unique physical properties, specific surface area, low risk of cytotoxicity, and excellent biological properties. This review is focused on nanocellulose based systems acting as carriers to be used in drug or antimicrobial delivery by providing different but controlled and sustained release of drugs or antimicrobial agents, respectively, thus showing potential for different routes of applications and administration. Microorganisms are increasingly resistant to antibiotics, and because, generally, the used metal or metal oxide nanoparticles at some concentration have toxic effects, more research has focused on finding biocompatible antimicrobial agents that have been obtained from natural sources. Our review contains the latest research from the last five years that tested nanocellulose-based materials in the field of drug delivery and antimicrobial activity.

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