Theatre Audience Group Suggestions and the Individual Specimen

1981 ◽  
Vol 7 (2-3) ◽  
Author(s):  
Ingvar Holm
1962 ◽  
Vol S7-IV (6) ◽  
pp. 816-825
Author(s):  
Henri Rouvier

Abstract The mineralization of Diois and Baronnies (France) is examined on the individual specimen, bed, and regional levels. The mineral composition consists essentially of zinc blende, galena and pyrite with some marcasite in a calcitic gangue. Study of galena and calcite samples indicates a low temperature of deposition. Three phases of mineral concentration are hypothesized; precipitation of water-dissolved metallic ions by hydrosulfuric acid; upper Eocene deposition of lead and zinc concentrations along faults by ground water; and upper Miocene to Recent superficial alteration of the beds.


Author(s):  
Zhengzhe Wu ◽  
Jere Kahanpää ◽  
Pasi Sihvonen ◽  
Anne Koivunen ◽  
Hannu Saarenmaa

Digitisation of natural history collections draws increasing attention. The digitised specimens not only facilitate the long-term preservation of biodiversity information but also boost the easy access and sharing of information. There are more than two billion specimens in the world’s natural history collections and pinned insect specimens compose of more than half of them (Tegelberg et al. 2014, Tegelberg et al. 2017). However, it is still a challenge to digitise pinned insect specimens with current state-of-art systems. The slowness of imaging pinned insects is due to the fact that they are essentially 3D objects and associated labels are pinned under the insect specimen. During the imaging process, the labels are often removed manually, which slows down the whole process. How can we avoid handling the labels pinned under often fragile and valuable specimens in order to increase the speed of digitsation? In our work (Saarenmaa et al. 2019) for T3.1.2 task in the ICEDIG (https://www.icedig.eu) project, we first briefly reviewed the state-of-the-art approaches on small insect digitisation. Then recent promising technological advances on imaging were presented, some of which have not yet been used for insect digitisation. It seems that one single approach will not be enough to digitise all insect collections efficiently. The approach has to be optimized based on the features of the specimens and their associated labels. To obtain a breakthrough in insect digitisation, it is necessary to utilize a combination of existing and new technologies in novel workflows. To explore the options, we identified six approaches for digitising pinned insects with the goal of minimum manipulations of labels as follows. Minimal labels: Image selected individual specimens without removing labels from the pin by using two cameras. This method suits for small insects with only one or a few well-spaced labels. Multiple webcams: Similar to the minimal labels approach, but with multiple webcams at different positions. This has been implemented in a prototype system with 12 cameras (Hereld et al. 2017) and in the ALICE system with six DSLR cameras (Price et al. 2018). Imaging of units: Similar to the multiple webcams approach, but image the entire unit (“Units” are small boxes or trays contained in drawers of collection cabinets, and are being used in most major insect collections). Camera in robot arm: Image the individual specimen or the unit with the camera mounted at a robot arm to capture large number of images from different views. Camera on rails: Similar to camera in robot arm approach, but the camera is mounted on rails to capture the unit. A 3D model of the insects and/or units can be created, and then labels are extracted. This is being prototyped by the ENTODIG-3D system (Ylinampa and Saarenmaa 2019). Terahertz time-gated multispectral imaging: Image the individual specimen with terahertz time-gated multispectral imaging devices. Minimal labels: Image selected individual specimens without removing labels from the pin by using two cameras. This method suits for small insects with only one or a few well-spaced labels. Multiple webcams: Similar to the minimal labels approach, but with multiple webcams at different positions. This has been implemented in a prototype system with 12 cameras (Hereld et al. 2017) and in the ALICE system with six DSLR cameras (Price et al. 2018). Imaging of units: Similar to the multiple webcams approach, but image the entire unit (“Units” are small boxes or trays contained in drawers of collection cabinets, and are being used in most major insect collections). Camera in robot arm: Image the individual specimen or the unit with the camera mounted at a robot arm to capture large number of images from different views. Camera on rails: Similar to camera in robot arm approach, but the camera is mounted on rails to capture the unit. A 3D model of the insects and/or units can be created, and then labels are extracted. This is being prototyped by the ENTODIG-3D system (Ylinampa and Saarenmaa 2019). Terahertz time-gated multispectral imaging: Image the individual specimen with terahertz time-gated multispectral imaging devices. Experiments on selected approaches 2 and 5 are in progress and the preliminary results will be presented.


2021 ◽  
Vol 7 (2) ◽  
pp. 105-108
Author(s):  
Thomas S. Rau ◽  
Jakob Cramer ◽  
M. Geraldine Zuniga ◽  
Georg Böttcher ◽  
Thomas Lenarz

Abstract Cochlear implants include an electrode array (EA) which needs to be inserted into the cochlea. Insertion tests using artificial cochlear models (ACM) or ex vivo specimens are widely used methods during EA development to characterize EA design properties, including insertion forces. Measured forces are directly linked to the orientation of the cochlear lumen with respect to the insertion axis of the test bench. While desired insertion directions in ACM experiments can be predefined by design, specimens are individually shaped and the cochlear lumen is embedded invisibly. Therefore, a new method for accurate, individual specimen positioning is required. A key element of the proposed method is a customizable pose setting adapter (PSA) used to adjust the specimen’s fine positioning. After rigid fixation of the specimen to a holder featuring spherical registration markers and subsequent cone beam computed tomography the desired insertion direction is planned. The planned data is used to calculate the individual shape of the PSA. Finally, the PSA is 3D printed and mounted between force sensor and specimen holder to correctly align the specimen to the test bench’s insertion axis. All necessary hard- and software have been developed including the specimen holder, a software for registration and trajectory planning, and a custom Matlab script whose output drives a parametric CAD file of the PSA. Positioning accuracy was determined in a first trial using 10 virtual trajectories and was found to be 0.23 ± 0.12 mm and 0.38 ± 0.17°. The presented stereotactic positioning procedure enables high repeatability in future ex vivo insertion experiments due to accurate, image-guided control of the insertion direction.


Sexual Health ◽  
2020 ◽  
Vol 17 (1) ◽  
pp. 15 ◽  
Author(s):  
Steven G. Badman ◽  
Sara F. E. Bell ◽  
Judith A. Dean ◽  
Jime Lemoire ◽  
Luke Coffey ◽  
...  

Background The aim of this study was to compare the performance of pooled self-collected urogenital, pharyngeal and anorectal specimens to that of individual specimen results for the molecular detection of Chlamydia trachomatis (CT) and Neisseria gonorrhoeae (NG) near the point of care (POC) for diagnostic sensitivity. Methods: Clients (mostly men who have sex with men) attending an urban community testing service and three sex-on-premises venues in Brisbane, Australia, were offered CT and NG testing by trained lay providers. Participants provided three self-collected specimens (urine, pharyngeal and rectal) for testing by GeneXpert (Cepheid, Sunnyvale, CA, USA). If any of the individual specimens from a participant were positive, all three specimens were pooled and retested. Results: Of the 388 participants who provided three individual anatomical specimens, 76 (19.6%) were found to be positive for CT and/or NG at one or more sites. The pooling approach failed to detect five CT rectal and four NG pharyngeal infections. The overall performance (sensitivity) of the pooling approach compared with individual specimen testing and Cohen’s κ were 90.0% and 0.86 respectively for CT and 89.7% and 0.89 respectively for NG. Conclusions: Reduced sensitivity was observed when using pooled specimens for the detection of CT and NG using GeneXpert near the POC, similar to results reported in laboratory-based CT and NG pooling studies. These data suggest specimen pooling is feasible near to the POC, potentially saving time and costs when screening at-risk populations for CT and NG. Our data also suggest a reduction in pooled urine could improve overall test sensitivity.


1966 ◽  
Vol 3 (2) ◽  
pp. 139-161 ◽  
Author(s):  
J. L. Roy

Thermal demagnetization of red beds (109 specimens, 42 sites) from the Maritimes and Gaspé shows that the fossil magnetization consists of a large, thermally stable component and of small, unstable components that disappear at about 400 °C. Thermal cleaning of the whole collection was therefore carried out at 450 °C to demagnetize these soft components. This procedure changes the mean declination and inclination by 6° and 7° respectively and also changes the distribution of directions about the mean. A statistical analysis shows that the site mean directions (after demagnetization) obey a Fisher's (1953) distribution whereas the individual specimen directions do not. This result is of interest in two connections: first, it shows that, in this instance, the use of Fisher's (1953) statistics to analyse the results is not permissible when unit weight is given to the specimen, but that their use is legitimate when unit weight is given to the site; second, the result is consistent with the hypothesis that the earth's field, when sampled in this way (paleomagneticalty), can be represented by an axial geocentric dipole field perturbed by randomly directed magnetic components at the earth's surface. Comparison with other results available for this geological period shows a certain divergence among the paleomagnetic poles for North America. It is suggested that a partial demagnetization of these earlier collections might reduce the observed divergence.


Author(s):  
Tilo Henning ◽  
Patrick Plitzner ◽  
Andreas Müller ◽  
Anton Güntsch ◽  
Walter G. Berendsohn ◽  
...  

Herbarium specimens are central to botanical science and of rising importance thanks to increasing accessibility and broadened usability. Alongside the many new uses of specimen data, sit a range of traditional uses supporting the collection of morphological data and their application to taxonomy and systematics. (Henning et al. 2018). Technical workflows are needed to support the sustainable collection of this traditional information and maintain the high quality of the morphological data. Data exchange and re-usability requires the use of accepted controlled vocabularies (community approved) that are accessible (web-based ontologies and term vocabularies) and reliable (long-term availability/unique identifiers). The same applies to datasets that must be stored accessibly and sustainably by maintaining all data relationships that would facilitate convenient re-use. This project aims to construct a comprehensive workflow to optimise the delimitation and characterisation (“descriptions”) of taxa (see complementary talk by Plitzner et al.). It is implemented on the open-source software framework of the EDIT Platform for Cybertaxonomy (http://www.cybertaxonomy.org, Ciardelli et al. 2009) extending the workflow for sample data processing developed in a preceding project (Kilian et al. 2015). The principal goals of this new software component are: specimen-level recording and storage of character data in structured character matrices generating taxon characterisations by aggregating the individual specimen-based datasets using and developing community-coordinated, ontology-based exemplar vocabularies persistently linking character datasets with source specimens for high visibility and re-usability specimen-level recording and storage of character data in structured character matrices generating taxon characterisations by aggregating the individual specimen-based datasets using and developing community-coordinated, ontology-based exemplar vocabularies persistently linking character datasets with source specimens for high visibility and re-usability The angiosperm order, Caryophyllales, provides an exemplar use case through cooperation with the Global Caryophyllales Initiative (Borsch et al. 2015). A basic set of morphological terms and vocabularies has been obtained from various online sources (ontologies, glossaries) and can be used, searched and expanded in the EDIT platform. The terms are categorised into: structures, properties and states. Different editors have been developed to combine structure and property terms to characters and assign a customised state vocabulary (categorical) or suitable values and units (numerical) to them. The workflow is built around a data set defining the taxonomic environment of individual use cases. A data set is specified by the characters and a taxonomic group, which can be filtered by area or rank. The dataset can be opened in a tabular representation (character matrix) to enter preselected state terms or values for the individual specimen. The matrix provides several features for basic comparison and analysis and allows the entry of alternative datasets (e.g. literature). Finally, the aggregation of data subsets to potential taxonomic units by adding up the values and summarising character states, allows the convenient test of taxonomic hypotheses. The term additivity is used here to describe this set of workflows and processes adding value to herbarium specimens and accumulating the specimen data for a taxon description.


Author(s):  
C.N. Sun

The present study demonstrates the ultrastructure of the gingival epithelium of the pig tail monkey (Macaca nemestrina). Specimens were taken from lingual and facial gingival surfaces and fixed in Dalton's chrome osmium solution (pH 7.6) for 1 hr, dehydrated, and then embedded in Epon 812.Tonofibrils are variable in number and structure according to the different region or location of the gingival epithelial cells, the main orientation of which is parallel to the long axis of the cells. The cytoplasm of the basal epithelial cells contains a great number of tonofilaments and numerous mitochondria. The basement membrane is 300 to 400 A thick. In the cells of stratum spinosum, the tonofibrils are densely packed and increased in number (fig. 1 and 3). They seem to take on a somewhat concentric arrangement around the nucleus. The filaments may occur scattered as thin fibrils in the cytoplasm or they may be arranged in bundles of different thickness. The filaments have a diameter about 50 A. In the stratum granulosum, the cells gradually become flatted, the tonofibrils are usually thin, and the individual tonofilaments are clearly distinguishable (fig. 2). The mitochondria and endoplasmic reticulum are seldom seen in these superficial cell layers.


Author(s):  
Anthony J. Godfrey

Aldehyde-fixed chick retina was embedded in a water-containing resin of glutaraldehyde and urea, without dehydration. The loss of lipids and other soluble tissue components, which is severe in routine methods involving dehydration, was thereby minimized. Osmium tetroxide post-fixation was not used, lessening the amount of protein denaturation which occurred. Ultrathin sections were stained with 1, uranyl acetate and lead citrate, 2, silicotungstic acid, or 3, osmium vapor, prior to electron microscope examination of visual cell outer segment ultrastructure, at magnifications up to 800,000.Sections stained with uranyl acetate and lead citrate (Fig. 1) showed that the individual disc membranes consisted of a central lipid core about 78Å thick in which dark-staining 40Å masses appeared to be embedded from either side.


Author(s):  
Anthony A. Paparo ◽  
Judith A. Murphy

The purpose of this study was to localize the red neuronal pigment in Mytilus edulis and examine its role in the control of lateral ciliary activity in the gill. The visceral ganglia (Vg) in the central nervous system show an over al red pigmentation. Most red pigments examined in squash preps and cryostat sec tions were localized in the neuronal cell bodies and proximal axon regions. Unstained cryostat sections showed highly localized patches of this pigment scattered throughout the cells in the form of dense granular masses about 5-7 um in diameter, with the individual granules ranging from 0.6-1.3 um in diame ter. Tissue stained with Gomori's method for Fe showed bright blue granular masses of about the same size and structure as previously seen in unstained cryostat sections.Thick section microanalysis (Fig.l) confirmed both the localization and presence of Fe in the nerve cell. These nerve cells of the Vg share with other pigmented photosensitive cells the common cytostructural feature of localization of absorbing molecules in intracellular organelles where they are tightly ordered in fine substructures.


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