scholarly journals Green synthesized silver and copper nanoparticles induced changes in biomass parameters, secondary metabolites production, and antioxidant activity in callus cultures of Artemisia absinthium L.

2021 ◽  
Vol 10 (1) ◽  
pp. 61-72
Author(s):  
Khizar Hayat ◽  
Shahid Ali ◽  
Saif Ullah ◽  
Yujie Fu ◽  
Mubashir Hussain
Keyword(s):  
Antioxidant Activity ◽  
Copper Nanoparticles ◽  
Biomass Accumulation ◽  
Callus Cultures ◽  
Total Phenolic ◽  
Ag Nps ◽  
Artemisia Absinthium ◽  
Culture Production ◽  
Metabolites Production ◽  
Induced Changes

Abstract Artemisia absinthium L. is a highly medicinal plant with a broad range of biomedical applications. A. absinthium callus cultures were established in response to bio-fabricated single NPs (Ag and Cu) or a combination of both NPs (Ag and Cu) in different ratios (1:2, 2:1, 1:3, and 3:1) along with thidiazuron (TDZ) (4 mg/L) to elicit the biomass accumulation, production of non-enzymatic compounds, antioxidative enzymes, and antioxidant activity. Silver and copper nanoparticles (Ag and Cu NPs) were synthesized using the leaves of Moringa oleifera as reducing and capping agent and further characterized through UV-Visible spectroscopy and SEM. The 30 µg/L suspension of Ag and Cu NPs (1:2, 2:1) and 4 mg/L TDZ showed 100% biomass accumulation as compared to control (86%). TDZ in combination with Ag NPs enhanced biomass in the log phases of growth kinetics. The Cu NPs alone enhanced the superoxide dismutase activity (0.56 nM/min/mg FW) and peroxidase activity (0.31 nM/min/mg FW) in callus cultures. However, the combination of Ag and Cu NPs with TDZ induced significant total phenolic (7.31 µg/g DW) and flavonoid contents (9.27 µg/g DW). Furthermore, the antioxidant activity was highest (86%) in the Ag and Cu NPs (3:1) augmented media. The present study provides the first evidence of bio-fabricated single NPs (Ag and Cu) or a combination of both NPs (Ag and Cu) in different ratios (1:2, 2:1, 1:3, and 3:1) along with TDZ (4 mg/L) on the development of callus culture, production of endogenous enzymes, non-enzymatic components, and further antioxidant activity in callus cultures of A. absinthium.

scholarly journals INFLUENCE OF AUXIN ON IN VITRO SECONDARY METABOLITES PRODUCTION FROM BALANITES AEGYPTIACA CALLUS CULTURES

2021 ◽  
Vol 21 (2) ◽  
Author(s):  
Asmaa A.A. Abdel- Kareem ◽  
H.A. El- Shamy ◽  
A.K. Dawh ◽  
S.G. Gwiefel
Keyword(s):  
Secondary Metabolites ◽  
Callus Cultures ◽  
Total Phenolic ◽  
Total Flavonoids ◽  
Ms Medium ◽  
Total Phenolic Compounds ◽  
Fresh Weight ◽  
Balanites Aegyptiaca ◽  
Metabolites Production

The present work was conducted in order to investigate the effect of auxin type (2,4-D and NAA) and concentration (0.00, 0.25, 0.50, 1.00 and 2.00 mg/l) on Balanites aegyptiaca callus cultures growth and production of secondary metabolites. Obtained results demonstrated that supplementation MS medium with 2,4-D at 2.0 mg/l could enhanced and recorded the ultimate values of callus fresh weight, antioxidant activity (%), total flavonoids, total phenolic compounds and total saponins contents and yields of Balanites aegyptiaca L. callus.

scholarly journals Polyphenol contents and antioxidant activity of carob callus cultures

2021 ◽  
pp. e652
Author(s):  
Assia Lozzi ◽  
Rachid Mentag ◽  
Driss Alami-Halimi ◽  
Rabha Abdelwahd ◽  
Abdelhadi Abousalim
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Activities ◽  
Correlation Coefficients ◽  
Biomass Accumulation ◽  
Callus Cultures ◽  
Lower Amount ◽  
Medicinal Uses ◽  
Light Regimes ◽  
Enhanced Production ◽  
Mediterranean Plant

Carob (Ceratonia siliqua L.) is an important Mediterranean plant species with worldwide commercial and medicinal uses. The establishment of a callus culture protocol as an alternative system to produce polyphenols of chemical and pharmaceutical interest was made in the present study for the first time in carob. Explant type and the light regime are two important factors that influence morphogenic responses and biochemical production. Maximal callus induction (100 %) and biomass accumulation were obtained in cotyledon explants under both tested light regimes (16-hour photoperiod and darkness). However, leaf callus produced a higher amount of polyphenols (TPC) and flavonoids (TFC) but a lower amount of total condensed tannins (TCT) as compared to cotyledon callus. Light treatment has significantly increased TCT content but decreased the antioxidant activity in carob callus cultures. Strong and positive correlations were obtained between TPC and TFC and the antioxidant activities with correlation coefficients ranging between 0.68 and 0.98. The obtained results indicate that calli of C. siliqua have the potential for enhanced production of phenolic compounds with antioxidant activity that is favored by culture under dark condition.

scholarly journals Evaluation of Total Phenolic Contents and Antioxidant Activity of Carrot Callus Extracts as Affected by Phenylalanine Precursor

2016 ◽  
Vol 25 (2) ◽  
pp. 207-221 ◽  
Author(s):  
Nermeen M Arafa ◽  
Mona M Ibrahim ◽  
Usama Ibrahim Aly
Keyword(s):  
Antioxidant Activity ◽  
Daucus Carota ◽  
Light Condition ◽  
Callus Cultures ◽  
Total Phenolic ◽  
Scavenging Activity ◽  
Light Conditions ◽  
Phenolic Contents ◽  
Total Phenolic Contents ◽  
Dark Conditions

Carrot (Daucus carota) is a valuable plant with both therapeutic and horticultural potential. Stem, petiole and root derived calli of carrot were obtained on solid MS supplemented with 1 mg/l BAP + 2 mg/l NAA. Callus cultures supplemented with different L-phenylalanine (PHE) concentrations under light and dark conditions were evaluated for their antioxidant activity and total phenolic contents. The authors showed that PHE supplementation in Daucus carota cultures was necessary to raise the extraction yield percentage. Antioxidant assays such as DPPH scavenging activity and ?-carotene bleaching have been carried out. In DPPH system, callus extracts from different explants grown under light conditions displayed lower DPPH radical scavenging activity at all PHE levels compared with that grown under dark conditions. Moreover, under both light and dark conditions callus cultures grown on MS supplemented with 1 mg/l BAP + 2 mg/l NAA plus 1000 mg/l PHE were recorded to yield the maximum values as antioxidant activities. Regarding ?-carotene bleaching assay, under light condition the callus extract of stem, root and petiole recorded an inhibition of linolic acid 47.9, 41.43 and 39%) which is lower compared with dark grown cultures, respectively (52.46, 72.71 and 73.26%). Effect of different concentrations of phenylalanine on the total phenolic content of carrot callus extract examined under light conditions varied from 0.33 to 2 mg/g DW and 0.51 to 3.69 mg/g DW under dark conditions as expressedPlant Tissue Cult. & Biotech. 25(2): 207-221, 2015 (December)

scholarly journals Optimization of Biomass Accumulation and Production of Phenolic Compounds in Callus Cultures of Rhodiola rosea L. Using Design of Experiments

Plants ◽  
2022 ◽  
Vol 11 (1) ◽  
pp. 124
Author(s):  
Anna A. Erst ◽  
Anastasia A. Petruk ◽  
Andrey S. Erst ◽  
Denis A. Krivenko ◽  
Nadezhda V. Filinova ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
Plant Growth ◽  
Design Of Experiments ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Biomass Accumulation ◽  
Callus Cultures ◽  
Rhodiola Rosea ◽  
Total Phenolic

Rhodiola rosea L. is a valuable medicinal plant with adaptogenic, neuroprotective, antitumor, cardioprotective, and antidepressant effects. In this study, design of experiments methodology was employed to analyze and optimize the interacting effects of mineral compounds (concentration of NO3− and the ratio of NH4+ to K+) and two plant growth regulators [total 6-benzylaminopurine (BAP) and α-naphthylacetic acid (NAA) concentration and the ratio of BAP to NAA] on the growth and the production of total phenolic compounds (TPCs) in R. rosea calluses. The overall effect of the model was highly significant (p < 0.0001), indicating that NH4+, K+, NO3−, BAP, and NAA significantly affected growth. The best callus growth (703%) and the highest production of TPCs (75.17 mg/g) were achieved at an NH4+/K+ ratio of 0.33 and BAP/NAA of 0.33, provided that the concentration of plant growth regulators was 30 μM and that of NO3− was ≤40 mM. According to high-performance liquid chromatography analyses of aerial parts (leaves and stems), in vitro seedlings and callus cultures of R. rosea contain no detectable rosarin, rosavin, rosin, and cinnamyl alcohol. This is the first report on the creation of an experiment for the significant improvement of biomass accumulation and TPC production in callus cultures of R. rosea.

Evaluation of Growth and Antioxidant Activity in Suaeda monoica and Suaeda nudiflora Callus Cultures under Sequential Exposure to Saline Conditions

2019 ◽  
Vol 8 (1) ◽  
pp. 42-52
Author(s):  
Abhishek Joshi ◽  
Bhanupriya Kanthaliya ◽  
Jaya Arora
Keyword(s):  
Antioxidant Activity ◽  
Callus Culture ◽  
Antioxidant Activities ◽  
Callus Cultures ◽  
Total Phenolic ◽  
Specific Cell ◽  
Strong Positive Correlation ◽  
Ms Medium ◽  
Callus Regeneration

Background: Plant in vitro culture systems serve as a useful tool to study the regulatory routes which are related to plant growth and survival under altered environmental conditions. Methods: Callus culture of Suaeda monoica and Suaeda nudiflora were established for studying the salt tolerance mechanism at the cellular level. Calli of both the species were induced from seedling’s epicotyls on Murashige and Skoog (MS) medium supplemented with a different combination of auxin and cytokinins. A sequential stress treatment was given to the callus of both the species. The growth rate of callus, osmolytes and antioxidant activities was investigated after 28 days. A control callus was maintained in each experiment without any salt in the growth medium. Results: Efficient callus regeneration was obtained by exposing the callus tissue to MS medium supplemented with 2,4-dichlorophenoxyacetic acid (2,4-D, 1.0 mg/l), benzylaminopurine (BAP, 0.5 mg/l) and 2,4-D (0.5 mg/l), kinetin (Kn, 0.25 mg/l) for S. monoica and S. nudiflora, respectively. A substantial increase was observed in proline content and a strong positive correlation was found between the total phenolic content and antioxidant activity under increasing salt concentrations. Conclusion: This is the first report on S. monoica callus regeneration. The specific cell lines which were generated through callus culture under sequential saline conditions provide a promising foundation for studying salinity induced expression of enzymes. Further comparison of transcriptomic profiles of control and salt-treated callus cultures can serve as a promising system for the detection of genes responsible for the change in expression under salt stress.

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