Assessment of Genetic Diversity of Cultivated Sunflower in Terms of Oil Content, Fatty Acid Compositions and Seed Traits

Abstract Sunflower (Helianthus annuus L.) cultivated accessions contains useful genes encoding different phenotypic characteristic through which the origin of sunflower oil could be hypothesized. Those genes could be later used for future breeding programs for providing better quality sunflower oil. The objective of the current study is to discriminate genetic diversity of cultivated sunflower seeds collection through the statistical methods such as PCA (principal component analysis) and Pearson correlation analysis for two characters; seed oil content and fatty acid composition. Materials and methods: In the present study, the genetic diversity of 107 cultivated accessions of Helianthus annuus L. was studied for fatty acid composition and oil content. Pearson correlation and Principal Component Analysis (PCA) were used to determine the correlation between the studied parameters. A dendrogram using Ward’s method and the squared Euclidean distance coefficient was produced. The results showed that the average seed oil content in the accessions was 29.51 % with a profile of 7.23 % palmitic acid (PAL), 5.04 % stearic acid (STE), 36.85 % oleic acid (OLE) and 50.85 % linoleic acid (LIN). The highest oil content was found in accession Hopi Dye (43.66 %). High levels of OLE were observed in the Csehszlovakiai “B” (60.14 %) and Vk-47 (55.73 %) accessions. On the other hand, Fuksinka 10 and Georgia accessions had the lowest mean PAL values (4.98 %) and STE (1.81 %), respectively. Palmitoleic acid (PALM) was identified in 29 accessions and linolenic acid (LIL) in 32 accessions, the highest in Gonondu (0.86 %) and Oleisty Borovskil (0.76 %), respectively. A significant negative relationship between OLE, STE and saturated fatty acids (SFA) with oil content was observed. The Pearson correlation of unsaturated fatty acids to saturated fatty acids ratio (UFA/SFA ratio) with oil content was positive and significant. In the PCA analysis, four major principal components (PCs) were identified, accounting for 87.19 % of the total variations. In PC1, PAL and STE (with positive coefficients) and UFA/SFA ratio (with negative coefficients), had the highest loadings, which determined 41.33 % of the total variations. In PC 2, OLE (with negative coefficient) and LIN (with positive coefficient), had the highest values. According to the dendrogram of the accessions, they were grouped into seven distinct clusters and the accessions in clusters 4 and 7 contained high UFA and low SFA values. The findings of this study showed that there is a significant genetic diversity among the accessions, which can be used to maximize heterosis in sunflower breeding programs.

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Oil content, lipid profiling and oxidative stability of “Sefri” Moroccan pomegranate (Punica granatum L.) seed oil

OCL ◽

10.1051/ocl/2020069 ◽

2021 ◽

Vol 28 ◽

pp. 5

Author(s):

Ahmed Hajib ◽

Issmail Nounah ◽

Hicham Harhar ◽

Said Gharby ◽

Badreddine Kartah ◽

...

Keyword(s):

Fatty Acids ◽

Fatty Acid ◽

Oxidative Stability ◽

Oil Content ◽

Seed Oil ◽

Saturated Fatty Acids ◽

Punica Granatum ◽

Major Constituent ◽

Punicic Acid ◽

Pomegranate Seed

The aim of this study was to determine the chemical composition (fatty acids, tocopherols, and sterols) and evaluate the oxidative stability of Moroccan pomegranate (Punica granatum L.) seed oil. The oil content of pomegranate seed was 22.63 g/100g of dry weight. The fatty acid composition showed a dominance of conjugated linolenic acids (CLnAs) (86.96 g/100g). The most dominant fatty acid was punicic acid (75.1 g/100g), followed by catalpic acid (6.7 g/100g) and linoleic acid with amounts of 4.11 g/100g. The seed oil only contained a low level of saturated fatty acids with palmitic (2.64 g/100g) and stearic acids (1.73 g/100g) as main saturated fatty acids. The sterol marker, β-sitosterol, accounted for 404.59 mg/100g of the total sterol content in the seed oil. Total tocopherol content in seed oil was 332.44 mg/100g. γ-tocopherol (190.47 mg/100g oil) is the major constituent, followed by α-tocopherol (74.62 mg/100g oil) and δ-tocopherol (53.3 mg/100g oil). The induction time calculated by the Rancimat accelerated method was found to be of 3.6 h at 120 °C. In terms of oil, pomegranate seed oil may be considered as a valuable source for new multipurpose products with industrial, cosmetic and pharmaceutical uses.

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Effects of Genotype and Climatic Conditions on the Oil Content and Its Fatty Acids Composition of Carthamus tinctorius L. Seeds

Agronomy ◽

10.3390/agronomy11102048 ◽

2021 ◽

Vol 11 (10) ◽

pp. 2048

Author(s):

Kamel Zemour ◽

Ahmed Adda ◽

Amina Labdelli ◽

Abdelkader Dellal ◽

Muriel Cerny ◽

...

Keyword(s):

Fatty Acids ◽

High Temperatures ◽

Oil Content ◽

Seed Oil ◽

Unsaturated Fatty Acids ◽

Saturated Fatty Acids ◽

Carthamus Tinctorius ◽

Climatic Conditions ◽

Seed Oil Content ◽

Semi Arid

Safflower seeds provide an oil rich in mono and polyunsaturated fatty acids. Its adaptation to drought and high temperatures makes it an alternative for the development of oleaginous crops in semi-arid areas. This study examines the oil content and the chemical composition of seed oil from three safflower accessions (Gila, Halab, Touggourt) cultivated over three years (2015, 2016, and 2017) in a semi-arid area in Tiaret (West of Algeria). Under these semi-arid conditions, characterized by low rainfall and high temperatures, seed oil content remained relatively high and was composed mainly of unsaturated fatty acids, with their ratio to saturated fatty acids reaching an average value of 9. Seed oil content varies between 22.8% and 28.4% among the genotypes and throughout the three years. The extracted oil consists essentially of unsaturated fatty acids, linoleic and oleic acids, poly and monounsaturated, respectively. Their contents over the three years vary between 75% and 79.3% for linoleic acid and between 10.2% and 14.7% for oleic acid. The saturated fatty acids content of the oil reached a maximum value of 9%. They consist mainly of palmitic acid, of which the average genotypic content varies between 6.6% and 7.15% depending on all grown years. The results obtained may assist in better understanding the response of cultivars under rainfed conditions and could be helpful for breeders with regards to introduction into selection programs.

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Cultivar and Seasonal Effects on Seed Oil Content and Fatty acid Composition of Cucumber As a Potential Industrial Crop

Journal of the American Society for Horticultural Science ◽

10.21273/jashs.140.4.362 ◽

2015 ◽

Vol 140 (4) ◽

pp. 362-372 ◽

Cited By ~ 5

Author(s):

Joyce W. Ngure ◽

Chunyan Cheng ◽

Shuqiong Yang ◽

Qunfeng Lou ◽

Ji Li ◽

...

Keyword(s):

Fatty Acids ◽

Fatty Acid Composition ◽

Fatty Acid ◽

Acid Composition ◽

Oil Content ◽

Seed Oil ◽

Negative Relationship ◽

Oleic Acid Content ◽

Seed Oil Content ◽

Wide Range

Cucumber (Cucumis sativus) seed oil has the potential for use as an edible oil and as a pharmaceutical, cosmetic, insecticidal, and industrial product. In this study, we investigated, for the first time, the effect of cultivar and season on seed number, oil content, and fatty acid profiles as well as their proportions in different cucumber cultivars. We examined the effects of spring and autumn seasons on seed oil content and fatty acid composition in 46 cucumber cultivars and one wild species of cucumber (C. anguria) grown in greenhouse experiments in 2013 and 2014. Seed oil was determined using the Soxhlet method and fatty acids using the gas chromatography-mass spectrometry method. Seed oil content in the cucumber seeds ranged from 41.07% in ‘Hazerd’ to 29.24% in ‘Lubao’ while C. anguria had 23.3%. Fatty acids detected were linoleic (C18:2), palmitic (C16:0), oleic (C18:1), stearic (C18:0), linolenic (C18:3), behenic (C22:0), arachidic C20:0), lignoceric (C24:0), eicosenoic (C20:1), palmitoleic (C16:1), and myristic (C14:0), among other unidentified fatty acids. The results showed significant effects of cultivar genotype, growing season, and interactions on the variables examined. The content of seed oil and fatty acids differed significantly among the cultivar genotypes. Spring-grown cucumbers had higher quantities of oil than the autumn-grown cucumbers. The content of fatty acids (mainly palmitic, palmitoleic, stearic, oleic, eicosenoic, and lignoceric) also was higher in spring. In autumn there were more seeds, and higher linoleic, linolenic, and other unspecified fatty acids. The higher the oleic acid content the lower was the linoleic acid indicating a strong negative relationship in these two fatty acids. The higher the seed oil content the higher was linoleic and oleic indicating a positive relationship between the seed oil and the two fatty acids. Results of this study provide important information applicable in improving management and production of cucumber seed oil especially considering its versatility in uses. Furthermore, the wide range of fatty acids found in the studied cucumber cultivars could be used in the production of novel industrial oils through genetic engineering.

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Diversity in seed oil content and fatty acid composition in three tree peony species with potential as sources of omega-3 fatty acids

The Journal of Horticultural Science and Biotechnology ◽

10.1080/14620316.2015.1133538 ◽

2016 ◽

Vol 91 (2) ◽

pp. 175-179 ◽

Cited By ~ 17

Author(s):

Jigang Han ◽

Zhao Liu ◽

Xiaoqing Li ◽

Jian Li ◽

Yonghong Hu

Keyword(s):

Fatty Acids ◽

Fatty Acid Composition ◽

Fatty Acid ◽

Acid Composition ◽

Oil Content ◽

Seed Oil ◽

Omega 3 Fatty Acids ◽

Omega 3 ◽

Tree Peony ◽

Seed Oil Content

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Evaluation of the variability of citrullus colocynthis (l) schrad as potential biodiesel feedstock: oil content, oil yield and the fatty acid composition

E3S Web of Conferences ◽

10.1051/e3sconf/202130401002 ◽

2021 ◽

Vol 304 ◽

pp. 01002

Author(s):

Abdelhamid Benmoumou ◽

Saïd El Madidi

Keyword(s):

Fatty Acids ◽

Fatty Acid Composition ◽

Fatty Acid ◽

Acid Composition ◽

Oil Content ◽

Seed Oil ◽

Biodiesel Production ◽

Oil Yield ◽

Citrullus Colocynthis ◽

Seed Oil Content

Citrullus colocynthis has been garnering interest in recent times as a potential biodiesel feed stockcrop due to its high seed oil content and its natural adaptation to drought The variability seed oil content (OC), Oil yield (kg/ha) (OY) and fatty acid composition were investigated for 12 accessions collected in different localities in Morocco. Analysis of the data revealed high variability among the accessions, with seed oil content ranging between 17.1 and 24.3 % of seed weight and Oil yield from 35.3 to 172.7 kg/ha. The predominant fatty acid of the seed oil was linoleic acid, C18:2, ranging from 67.0 to 73.0 % of total fatty acids and the average values of unsaturated fatty acids vary between 75.25 and 81.94 %.The wide range of variations found in this study for the traits measured offers the possibility of genetic improvement to develop cultivars for biodiesel production compatible with sustainable and ecological agriculture.

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Seed oil content and fatty acid composition in a genebank collection of Cucurbita moschata Duchesne and C. argyrosperma C. Huber

Plant Genetic Resources ◽

10.1017/s1479262112000512 ◽

2013 ◽

Vol 11 (2) ◽

pp. 149-157 ◽

Cited By ~ 7

Author(s):

Robert L. Jarret ◽

Irvin J. Levy ◽

Thomas L. Potter ◽

Steven C. Cermak ◽

Laura C. Merrick

Keyword(s):

Fatty Acids ◽

Fatty Acid Composition ◽

Fatty Acid ◽

Acid Composition ◽

Seed Weight ◽

Oil Content ◽

Seed Oil ◽

Cucurbita Moschata ◽

Seed Oil Content ◽

Total Seed

Data on intra-specific variability for seed oil content, physical characteristics and fatty acid composition in Cucurbita moschata and Cucurbita argyrosperma are lacking in the scientific literature. We examined 528 genebank accessions of C. moschata and 166 accessions of C. argyrosperma – which included members of both subsp. argyrosperma and subsp. sororia – for seed oil content, oil physical characteristics and fatty acid composition. The oil of both species had near-identical viscosities, viscosity indices, colour and oxidative stabilities while the oil of C. argyrosperma had a slightly higher pour point, cloud point, percentage of free fatty acids and acid value when compared with C. moschata. Mean oil content values of the two species were similar at 28.7 ± /2.7 and 29.8 ± /2.6% for C. moschata and C. argyrosperma, respectively. The mean seed oil content of C. argyrosperma subsp. argyrosperma var. palmeri (32.1%) was significantly higher than that of the other taxa examined. The average (mean) percentage of total seed weight attributable to the kernel was 77.2% in C. moschata (n= 34) and 74.5% in C. argyrosperma (n= 46). The percentage of total seed weight attributable to the hull was correlated with seed oil content, in both species. Linoleic was the predominant fatty acid in all the samples analysed. Means for individual fatty acids in C. moschata were linoleic 48.5%, oleic 22.6%, palmitic 20.7% and stearic 7.5%. Means for individual fatty acids in C. argyrosperma were linoleic 47.3%, oleic 27.5%, palmitic 16.5% and stearic 8.0%.

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Development and screening of EMS mutants with altered seed oil content or fatty acid composition in Brassica napus

The Plant Journal ◽

10.1111/tpj.15003 ◽

2020 ◽

Vol 104 (5) ◽

pp. 1410-1422

Author(s):

Shan Tang ◽

Dong‐Xu Liu ◽

Shaoping Lu ◽

Liangqian Yu ◽

Yuqing Li ◽

...

Keyword(s):

Fatty Acid Composition ◽

Fatty Acid ◽

Brassica Napus ◽

Acid Composition ◽

Oil Content ◽

Seed Oil ◽

Seed Oil Content

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Chemical Properties and Fatty Acid Composition of Palado Seed Oil (Aglaia sp) Extracted Using Chloroform Solvent

Walailak Journal of Science and Technology (WJST) ◽

10.48048/wjst.2021.10587 ◽

2021 ◽

Vol 18 (4) ◽

Author(s):

Syamsul RAHMAN ◽

Salengke Salengke ◽

Abu Bakar TAWALI ◽

Meta MAHENDRADATTA

Keyword(s):

Fatty Acids ◽

Fatty Acid Composition ◽

Fatty Acid ◽

Acid Composition ◽

Seed Oil ◽

Unsaturated Fatty Acids ◽

Saturated Fatty Acids ◽

Chemical Properties ◽

Raw Material ◽

Food Ingredients

Palado (Aglaia sp) is a plant that grows wild in the forest around Mamuju regency of West Sulawesi, Indonesia. This plant is locally known as palado. Palado seeds (Aglaia sp) can be used as a source of vegetable oil because it contains approximately 14.75 % oil, and it has the potential to be used as food ingredients or as raw material for oil production. The purpose of this study was to determine the chemical properties and the composition of fatty acids contained in palado seed oil (Aglaia sp). The employed method involved the use of palado fruit that had been processed to be palado seed and undergoing flouring process. Palado flour was produced by the extraction process by using chloroform solvent with the soxhlet method. The characteristics of the chemical properties in the oil produced were analyzed by using a standard method, including iodine, saponification, and acid values. The analysis of fatty acid composition was conducted by using gas chromatography. The results showed that palado oil extracted with hexane had an iodine value of 15.38 mg/g, saponification value of 190.01 mg KOH/g, and acids value of 1.961 mg KOH/g. The fatty acid composition of the palado seed oil consisted of saturated fatty acids (41.601 %), which included palmitic acid (41.062 %), myristic acid (0.539 %), and unsaturated fatty acids (45.949 %), which included mono-unsaturated fatty acids (MUFA) such as (22.929 %), oleic acid and poly-unsaturated fatty acids (PUFA), which was linoleic acid (23.020 %).

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Cold-pressed cactus pear seed oil: Quality and stability

Grasas y Aceites ◽

10.3989/gya.0329201 ◽

2021 ◽

Vol 72 (3) ◽

pp. e415 ◽

Cited By ~ 3

Author(s):

M. De Wit ◽

V.K. Motsamai ◽

A. Hugo

Keyword(s):

Fatty Acids ◽

Fatty Acid Composition ◽

Fatty Acid ◽

Physicochemical Properties ◽

Acid Composition ◽

Oil Content ◽

Seed Oil ◽

Oil Quality ◽

Cactus Pear ◽

Cold Pressed

Cold-pressed seed oil from twelve commercially produced cactus pear cultivars was assessed for oil yield, fatty acid composition, physicochemical properties, quality and stability. Large differences in oil content, fatty acid composition and physicochemical properties (IV, PV, RI, tocopherols, ORAC, % FFA, OSI and induction time) were observed. Oil content ranged between 2.51% and 5.96% (Meyers and American Giant). The important fatty acids detected were C16:0, C18:0, C18:1c9 and C18:2c9,12, with C18:2c9,12, the dominating fatty acid, ranging from 58.56-65.73%, followed by C18:1c9, ranging between 13.18-16.07%, C16:0, which ranged between 10.97 - 15.07% and C18:0, which ranged between 2.62-3.18%. Other fatty acids such as C14:0, C16:1c9, C17:0, C17:1c10, C20:0, C18:3c9,12,15 and C20:3c8,11,14 were detected in small amounts. The quality parameters of the oils were strongly influenced by oil content, fatty acid composition and physicochemical properties. Oil content, PV, % FFA, RI, IV, tocopherols, ORAC and ρ-anisidine value were negatively correlated with OSI. C18:0; C18:1c9; C18:2c9,12; MUFA; PUFA; n-6 and PUFA/SFA were also negatively correlated with OSI. Among all the cultivars, American Giant was identified as the paramount cultivar with good quality traits (oil content and oxidative stability).

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