Composition and antifungal activity of balsam from Liquidambar formosana Hance

Holzforschung ◽  
2013 ◽  
Vol 67 (3) ◽  
pp. 345-351 ◽  
Author(s):  
Shih-Chang Chien ◽  
Jun-Hong Xiao ◽  
Yen-Hsueh Tseng ◽  
Yueh-Hsiung Kuo ◽  
Sheng-Yang Wang
Keyword(s):  
Antifungal Activity ◽  
Volatile Compounds ◽  
Tree Species ◽  
Brown Rot ◽  
Betulonic Acid ◽  
White Rot ◽  
Spectroscopic Techniques ◽  
Laetiporus Sulphureus ◽  
Lenzites Betulina

Abstract Formosan sweet gum (Liquidambar formosana Hance) is a tree species endemic in Taiwan. In this study, the composition of balsam from L. formosana has been determined by several chromatographic and spectroscopic techniques. Among the 26 compounds identified, three new triterpenoids were detected, namely, 2α,3α-dihydroxyolean-12-en-28-al (1), 3α-hydroxyolean-12-en-30-ol (2), and 3α-hydroxyolean-2-oxo-12-en-28-al (3). The most abundant volatile compounds were β-caryophyllene (22.7%), α-pinene (23.3%), and β-pinene (19.6%), and the most abundant nonvolatile compounds were 3α,25-dihydroxyolean-12-en-28-oic acid (12, 19.1%), oleanonic aldehyde (9, 14.0%), and betulonic acid (15, 13.4%). The compounds 3α,25-dihydroxyolean-12-en-28-oic acid and bornyl cinnamate were found to be inhibitory for white rot (Lenzites betulina) and brown rot (Laetiporus sulphureus) fungi.

scholarly journals Synthesis and activity evaluation of new benzofuran-1,3,4-oxadiazole hybrids against wood-degrading fungi

BioResources ◽  
2019 ◽  
Vol 15 (1) ◽  
pp. 1085-1097
Author(s):  
Seyyed Khalil Hosseinihashemi ◽  
Mahsa Toolabi ◽  
Fahimeh Abedinifar ◽  
Setareh Moghimi ◽  
Abbas Jalaligoldeh ◽  
...  
Keyword(s):  
Antifungal Activity ◽  
Trametes Versicolor ◽  
Antifungal Agents ◽  
Brown Rot ◽  
White Rot ◽  
Synthetic Pathway ◽  
Poria Placenta ◽  
Antifungal Potential ◽  
Antifungal Effects ◽  
Oxadiazole Derivatives

A series of novel benzofuran-1,3,4-oxadiazole hybrids were synthesized and evaluated as antifungal agents. The synthetic pathway was started from salicylaldehyde, which afforded 5-(substituted benzylthio)-1,3,4-oxadiazole derivatives in moderate to good yields. The compounds were investigated for their antifungal potential against white-rot, Trametes versicolor and brown-rot, Poria placenta and Coniophora puteana fungi at different concentrations (500, 1000 ppm). The obtaining results demonstrated that most of the compounds at 500 ppm concentration did not exhibit acceptable antifungal effects but they had better antifungal activity at 1000 ppm concentration. Compounds 5a, 5c, and 5i showed inhibition percentages of 14.6%, 23.0%, and 14.7%, against the growth of P. placenta and C. puteana, respectively. Among the compounds, the 2-(benzofuran-2-yl)-5-((2,6-difluorobenzyl)thio)-1,3,4-oxadiazole (5h) hybrid was the most active one.

Fungal wood decomposer activity induces niche separation between two dominant tree species seedlings regenerating on coarse woody material

2017 ◽  
Vol 47 (1) ◽  
pp. 106-112 ◽  
Author(s):  
Y. Fukasawa ◽  
Y. Komagata ◽  
S. Ushijima
Keyword(s):  
Tree Species ◽  
Linear Models ◽  
Pinus Densiflora ◽  
Wood Decay ◽  
Brown Rot ◽  
White Rot ◽  
Canopy Openness ◽  
Niche Separation ◽  
Seedling Regeneration ◽  
Coarse Woody Material

Decomposition subsystems have an essential role in forest dynamics but few studies demonstrate the effect of microbial decay traits on seedling regeneration. In the present study, we focused on seedling regeneration on coarse woody material (CWM), which is an important regeneration site for forest tree species, and the effects of wood decay type according to fungal decay preference for wood structural components on seedling colonization. Effects of log properties including wood decay type and other environmental variables on seedling density were evaluated by ordination methods and generalized linear models. In total, 22 woody species were recorded as seedlings on Pinus densiflora logs. By ordination analysis, white rot in heartwood and brown rot in sapwood, as well as canopy openness and log diameter, showed significant association with seedling communities. The factors selected for a generalized linear model for explaining seedling densities of the two dominant seedling species Cryptomeria japonica and P. densiflora included brown rot in sapwood and white rot in heartwood, but the effects were different: a positive effect of brown rot on C. japonica and a negative effect of white rot on P. densiflora. These results suggested that wood decay type could induce niche separation between dominant tree species regenerating on CWM.

scholarly journals Proteomics Reveals Octyl Gallate as an Environmentally Friendly Wood Preservative Leading to Reactive Oxygen Species-Driven Metabolic Inflexibility and Growth Inhibition in White-Rot Fungi (Lenzites betulina and Trametes versicolor)

2021 ◽  
Vol 7 (2) ◽  
pp. 145
Author(s):  
Jin-Wei Xu ◽  
Chen-Chung Liao ◽  
Ke-Chang Hung ◽  
Zhong-Yao Wang ◽  
Yu-Tang Tung ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Antifungal Activity ◽  
Trametes Versicolor ◽  
Reactive Oxygen ◽  
White Rot Fungi ◽  
Environmentally Friendly ◽  
White Rot ◽  
Oxygen Species ◽  
Wood Preservatives ◽  
Lenzites Betulina

The most commonly applied wood preservatives are based on creosote, pentachlorophenol, and waterborne chromate copper arsenate, which negatively affect the environment. Thus, environmentally friendly wood preservatives are required. This study investigated the antifungal activity and mechanism of several long-chain alkyl gallates (3,4,5-trihydroxybenzoates) against white-rot fungi, Lenzites betulina and Trametes versicolor. The results revealed that octyl gallate (OG) had the best antifungal activity. Additionally, OG may have a mechanism of action similar to surfactants and inhibit ATPase activity, causing mitochondrial dysfunction and endogenous reactive oxygen species (ROS) production. Upon exposure to endogenous ROS, cells rapidly inhibit the synthesis of 60S ribosomal subunits, thus reducing the mycelial growth rate. L. betulina and T. versicolor also remodeled their energy metabolism in response to low ATP levels and endogenous ROS. After OG treatment, ATP citrate synthase activity was downregulated and glycolytic activity was upregulated in L. betulina. However, the activity of aerobic pathways was decreased and the oxidative branch of the pentose phosphate pathway was redirected form nicotinamide adenine dinucleotide phosphate (NADPH) to minimize endogenous ROS-mediated damage in T. versicolor. Taken together, these observations reveal that OG is a potent inhibitor of white-rot fungus. Further structural optimization research and pharmacological investigations are warranted.

scholarly journals Antifungal activity of polycyclic aromatic hydrocarbons against Ligninolytic fungi

2011 ◽  
Vol 65 (5) ◽  
pp. 575-581 ◽  
Author(s):  
Mustafa Memic ◽  
Alisa Selovic ◽  
Jasmina Sulejmanovic
Keyword(s):  
Polycyclic Aromatic Hydrocarbons ◽  
Antifungal Activity ◽  
Aromatic Hydrocarbons ◽  
Fossil Fuels ◽  
Brown Rot ◽  
White Rot ◽  
Wood Preservation ◽  
Diffusion Method ◽  
Ligninolytic Fungi ◽  
Polycyclic Aromatic

Environmental contamination by polycyclic aromatic hydrocarbons (PAHs) has caused increasing concern because of their known, or suspected, carcinogenic and mutagenic effects. Polycyclic aromatic hydrocarbons occurring in the environment are usually the result of the incomplete combustion of carbon containing materials. The main sources of severe PAHs contamination in soil come from fossil fuels, i.e. production or use of fossil fuels or their products, such as coal tar and creosote. Creosote is used as a wood preservation for railway ties, bridge timbers, pilling and large-sized lumber. It consists mainly of PAHs, phenol and cresol compounds that cause harmful health effects. Research on biodegradation has shown that a special group of microorganisms, the white-rot fungi and brown-rot fungi, has a remarkable potential to degrade PAHs. This paper presents a study of the antifungal activity of 12 selected PAHs against two ligninolytic fungi Hypoxylon fragiforme (white rot) and Coniophora puteana (brown rot). The antifungal activity of PAHs was determined by the disc-diffusion method by measuring the diameter of the zone of inhibition. The results showed that the antifungal activity of the tested PAHs (concentration of 2.5 mmol/L) depends on the their properties such as molar mass, solubility in water, values of log Kow, ionization potential and Henry?s Law constant as well as number of aromatic rings, molecule topology or pattern of ring linkage. Among the 12 investigated PAHs, benzo(k) fluoranthene with five rings, and pyrene with four cyclic condensed benzene rings showed the highest antifungal activity.

scholarly journals Enzymatic Activities, Characteristics of Wood-Decay and Wood Substrate Specificity within Genera of Some Wood-Rotting Basidiomycetes from Cameroon and Tropical Africa

2022 ◽  
Vol 3 (1) ◽  
pp. 11-23
Author(s):  
Fabrice Tsigaing Tsigain ◽  
Blondo-Pascal Metsebing ◽  
Dominique Claude Mossebo ◽  
Leif Randulff Ryvarden ◽  
Romuald Oba ◽  
...  
Keyword(s):  
Substrate Specificity ◽  
Tree Species ◽  
Wood Decay ◽  
Brown Rot ◽  
Activity Level ◽  
White Rot ◽  
Final Conclusion ◽  
Polyphenol Oxidases ◽  
Different Types ◽  
Differential Test

A total of 72 species of wood-rotting Basidiomycetes belonging to 40 genera, 13 families and 5 orders, were investigated in this study of which 46 for the first time as far as type of wood-rot is concerned. 61 of the 72 cause white rot (W) representing 84.72 % of the total and 11 cause brown rot (B) or just 15.27 %, confirming the predominance already known of white rot (W) on brown rot (B) as shown in other studies. Results recorded show that even though most species belonging to same genera display the same type of rot (W or B), species of some few other genera were found to rather display different types of rot (W and B) in species within the same genera. Other results show that when determining the type of wood-rot caused by some species of fungi, in case of negative (-) test using tincture of guaiac which is known as the key test to determine the type of rot, syringaldazine must also be used as a differential test before concluding whether the species is a white (W) or brown rot (B) fungus. The level of activity of tyrosinase and peroxidase shows important variations between species of some genera, whereby some species of a given genus show varying potential level (+, ++, +++, ++++) of activities of these two enzymes, whereas other species of the same genus show no sign (-) of activity of the same enzymes. Therefore, our results led to the conclusion that the presence (+, ++, +++, ++++) or absence (-) of activity of peroxidase and tyrosinase can from now onwards also be used as an enzyme linked taxonomic criteria to distinguish between species of some genera. About detection of laccases activity, if a negative (-) result occurs during detection test in a wood-rotting fungus using syringaldazine, α-naphtol must also be used as a differential test before concluding on whether the species produces laccases or not. Based on the overall results recorded in the detection of enzymes activities, it appears necessary to use, where indicated, several substrates with different chemical sensitivities to detect the existence of an enzyme and its potential activity level in a fungal species. Additionally, preliminary lists of wood-decay fungi with potentially strong (+++, ++++) capacity to produce different types of polyphenol oxidases potentially usable in paper making industries, wastewater treatment and soil remediation, are provided. With regard to the study of substrate specificity which aimed at finding out the existence or not of a specificity between polyporales and tree wood species on which they grow, the first results recorded on a limited number of trees investigated led to the preliminary conclusion that, although some few tree species may serve as hosts for only a single species of polyporales, there is rather a greater tendency of finding several species growing on different species of wood as well as the same species of wood hosting several species of polyporales. These field observations led to the remark that a much larger inventory including a greater number of tree species in various tropical ecosystems is necessary in order to come out with a final conclusion.

Revalorizing Lignocellulose for the Production of Natural Pharmaceuticals and Other High Value Bioproducts

2019 ◽  
Vol 26 (14) ◽  
pp. 2475-2484 ◽  
Author(s):  
Congqiang Zhang ◽  
Heng-Phon Too
Keyword(s):  
Clostridium Thermocellum ◽  
White Rot Fungi ◽  
Brown Rot ◽  
Cost Effective ◽  
White Rot ◽  
Chemical Pretreatment ◽  
Significant Challenge ◽  
Drug Molecules ◽  
Natural Medicine ◽  
Renewable Natural Resource

Lignocellulose is the most abundant renewable natural resource on earth and has been successfully used for the production of biofuels. A significant challenge is to develop cost-effective, environmentally friendly and efficient processes for the conversion of lignocellulose materials into suitable substrates for biotransformation. A number of approaches have been explored to convert lignocellulose into sugars, e.g. combining chemical pretreatment and enzymatic hydrolysis. In nature, there are organisms that can transform the complex lignocellulose efficiently, such as wood-degrading fungi (brown rot and white rot fungi), bacteria (e.g. Clostridium thermocellum), arthropods (e.g. termite) and certain animals (e.g. ruminant). Here, we highlight recent case studies of the natural degraders and the mechanisms involved, providing new utilities in biotechnology. The sugars produced from such biotransformations can be used in metabolic engineering and synthetic biology for the complete biosynthesis of natural medicine. The unique opportunities in using lignocellulose directly to produce natural drug molecules with either using mushroom and/or ‘industrial workhorse’ organisms (Escherichia coli and Saccharomyces cerevisiae) will be discussed.

Growth behavior of wood-destroying fungi in chemically modified wood: wood degradation and translocation of nitrogen compounds

Holzforschung ◽  
2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Lukas Emmerich ◽  
Maja Bleckmann ◽  
Sarah Strohbusch ◽  
Christian Brischke ◽  
Susanne Bollmus ◽  
...  
Keyword(s):  
Protective Action ◽  
Treated Wood ◽  
Untreated Wood ◽  
Brown Rot ◽  
Decay Resistance ◽  
White Rot ◽  
Decay Fungi ◽  
Chemically Modified ◽  
Decay Tests ◽  
Modified Wood

Abstract Chemical wood modification has been used to modify wood and improve its decay resistance. However, the mode of protective action is still not fully understood. Occasionally, outdoor products made from chemically modified timber (CMT) show internal decay while their outer shell remains intact. Hence, it was hypothesized that wood decay fungi may grow through CMT without losing their capability to degrade non-modified wood. This study aimed at developing a laboratory test set-up to investigate (1) whether decay fungi grow through CMT and (2) retain their ability to degrade non-modified wood. Acetylated and 1,3-dimethylol-4,5-dihydroxyethyleneurea (DMDHEU) treated wood were used in decay tests with modified ‘mantle specimens’ and untreated ‘core dowels’. It became evident that white rot (Trametes versicolor), brown rot (Coniophora puteana) and soft rot fungi can grow through CMT without losing their ability to degrade untreated wood. Consequently, full volume impregnation of wood with the modifying agent is required to achieve complete protection of wooden products. In decay tests with DMDHEU treated specimens, significant amounts of apparently non-fixated DMDHEU were translocated from modified mantle specimens to untreated wood cores. A diffusion-driven transport of nitrogen and DMDHEU seemed to be responsible for mass translocation during decay testing.

scholarly journals Characterization of a Spirit Beverage Produced with Strawberry Tree (Arbutus unedo L.) Fruit and Aged with Oak Wood at Laboratorial Scale

2021 ◽  
Vol 11 (11) ◽  
pp. 5065
Author(s):  
Ofélia Anjos ◽  
Soraia Inês Pedro ◽  
Débora Caramelo ◽  
Andreia Semedo ◽  
Carlos A. L. Antunes ◽  
...  
Keyword(s):  
Volatile Compounds ◽  
Spectroscopic Techniques ◽  
Dry Extract ◽  
Oak Wood ◽  
Arbutus Unedo ◽  
Fusel Alcohols ◽  
Mediterranean Countries ◽  
Trained Panel ◽  
Valuable Product

Arbutus unedo spirit is a valuable product in Mediterranean countries. This spirit is usually marketed in Portugal without wood ageing. This work aims to characterize the ageing effect on the Arbutus unedo spirit, for three and six months with oak wood (Quercus robur L.) submitted to different toasting levels, based on its chemical composition and its sensory properties. For this purpose, several parameters were analysed: acidity, pH, dry extract, and volatile compounds (methanol, acetaldehyde, ethyl acetate and fusel alcohols). The volatile compounds were identified by GC-MS and quantified by GC-FID. Sensory analysis was performed by a trained panel, who have profiled this beverage, as well as the changes acquired during ageing. Spectroscopic techniques, namely FTIR–ATR, were applied to discriminate the different beverages produced. The results highlighted an increase in Arbutus unedo spirit’s quality with the wood contact, mainly based on the sensory attributes. Additionally, they showed that the best beverages were produced using oak wood with medium toasting levels during three months of ageing.

Detection of fungal decay by high-energy multiple impact (HEMI) testing

Holzforschung ◽  
2006 ◽  
Vol 60 (2) ◽  
pp. 217-222 ◽  
Author(s):  
Christian Brischke ◽  
Christian Robert Welzbacher ◽  
Andreas Otto Rapp
Keyword(s):  
Structural Changes ◽  
Brown Rot ◽  
High Energy ◽  
White Rot ◽  
Fungal Decay ◽  
Highly Correlated ◽  
Thermally Modified Wood ◽  
Impact Milling ◽  
Multiple Impact ◽  
Steel Balls

Abstract The suitability of a previously described high-energy multiple impact (HEMI) test for the detection of early fungal decay was examined. The HEMI test characterises the treatment severity of thermally modified wood by stressing the treated material by thousands of impacts of pounding steel balls. This method differentiates between heat treatment intensities, which are manifest as structural changes in the wood. Similar changes in wood structure are known for wood decayed by fungi. Pine (Pinus sylvestris L.) decayed by brown rot and beech (Fagus sylvatica L.) decayed by white rot were tested. Mass loss caused by fungal decay and resistance to impact milling (RIM) determined in HEMI tests were found to be highly correlated. Testing of non-degraded pine, beech, and ash (Fraxinus exelsior L.) showed only marginal effects of wood density on RIM. Furthermore, annual ring angles and RIM of spruce (Picea abies Karst.) were not correlated. Accordingly, the detection of RIM reduction in decayed wood is not masked by variations in density and orientation of the annual rings. Previous results showed no adverse effects of weathering on RIM. Thus, the detection of fungal decay with HEMI tests is feasible not only for laboratory purposes, but also for wood in outdoor applications that has already undergone weathering.

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