Obtention of Rosemary Essential Oil Concentrates by Molecular Distillation and Free Radical Scavenging Capacity Analysis

2013 ◽  
Vol 9 (2) ◽  
pp. 147-153 ◽  
Author(s):  
Gabriela Natalia Mezza ◽  
Ana V. Borgarello ◽  
Jorge D. Daguero ◽  
Maria C. Pramparo
Keyword(s):  
Essential Oil ◽  
Molecular Distillation ◽  
Radical Scavenging ◽  
Linalyl Acetate ◽  
Antioxidant Power ◽  
Food Industries ◽  
Radical Scavenging Capacity ◽  
Scavenging Capacity ◽  
Antibacterial And Antifungal ◽  
Rosemary Essential Oil

AbstractRosemary essential oil is used in pharmaceutical, cosmetic and food industries. Due to its constituents’ chemical activity, it is also used as antioxidant to preserve foods and as antibacterial and antifungal agents.The most abundant components of rosemary essential oil used in this work are α-pinene, myrcene, 1,8-cineole and camphor, which respond to 1,8-cineole chemotype. Two sets of molecular distillation experience were conducted. Antioxidant power of distillates and residues obtained was quantified, and the residues obtained from molecular distillation have more antioxidant power than distillates and rosemary essential oil.The results of this study show that it is feasible to use molecular distillation operation to obtain concentrates of rosemary essential oil. Residues collected present higher antioxidant power than rosemary essential oil, probably due to the presence of camphor, linalool, linalyl acetate and α-terpineol, which are present in higher proportion in the residues.

scholarly journals USE OF A FREE RADICAL SCAVENGING METHOD ON EXTRACTS OBTAINED BY MOLECULAR DISTILLATION FROM OREGANO ESSENTIAL OIL

2014 ◽  
Vol 44 (1) ◽  
pp. 25-30
Author(s):  
A. V. BORGARELLO ◽  
G. N. MEZZA ◽  
A. T. SOLTERMANN ◽  
M. C. PRAMPARO
Keyword(s):  
Free Radical ◽  
Essential Oil ◽  
Molecular Distillation ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Oregano Essential Oil ◽  
Radical Scavenging Capacity ◽  
Scavenging Capacity ◽  
Ic50 Values

There is evidence that oregano essential oil has a significant antioxidant effect on the process of lipid oxidation. In order to obtain fractions enriched in antioxidant properties, oregano essential oil samples were processed by molecular distillation. Molecular distillation experiments were done in two groups of tests, using an evaporating temperature between 22 and 30 ºC. Pressure was varied between 0.7 and 53 mbar. Antioxidant activity, specifically radical scavenging capacity, was analyzed in the essential oil and the distillates and residues obtained by a free radical scavenging method, using DPPH* (2,2-diphenyl-1picrylhydrazyl). The results obtained allowed to conclude that the free radical scavenging capacity was increased in the residue fractions obtained by molecular distillation. These fractions were concentrated in thymol. The IC50 values of the residues were similar to the synthetic antioxidant BHT, suggesting that fractions concentrated in oxygen derivatives obtained by molecular distillation could be used in the food industry to retard lipid degradation.

scholarly journals Phenolic Compounds, Antioxidant Activities, and Inhibitory Effects on Digestive Enzymes of Different Cultivars of Okra (Abelmoschus esculentus)

Molecules ◽  
2020 ◽  
Vol 25 (6) ◽  
pp. 1276
Author(s):  
Ding-Tao Wu ◽  
Xi-Rui Nie ◽  
Dan-Dan Shen ◽  
Hong-Yi Li ◽  
Li Zhao ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
Digestive Enzymes ◽  
High Performance ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Natural Antioxidants ◽  
Inhibitory Effects ◽  
Antioxidant Power ◽  
Radical Scavenging Capacity ◽  
Scavenging Capacity

In this study, the phenolic profiles and bioactivities of five representative cultivars of okra collected in China were investigated. Noticeable variations of phenolic compounds and their bioactivities were observed among these different cultivars of okra. The contents of total flavonoids (TFC) in “Shuiguo”, “Kalong 8”, “Kalong 3”, “Wufu”, and “Royal red” ranged from 1.75 to 3.39 mg RE/g DW, of which “Shuiguo” showed the highest TFC. Moreover, five individual phenolic compounds were found in okra by high performance liquid chromatography analysis, including isoquercitrin, protocatechuic acid, quercetin-3-O-gentiobioside, quercetin, and rutin, while isoquercitrin and quercetin-3-O-gentiobioside were detected as the main phenolic compounds in okra. Moreover, all tested okra exhibited significant antioxidant activities (2,2-diphenyl-1-picrylhydrazyl radical scavenging capacity, 2,2’-azino-bis (3-ethylenzthiazoline-6-sulphonic acid) radical scavenging capacity, and ferric reducing antioxidant power) and inhibitory effects on digestive enzymes (lipase, α-glucosidase, and α-amylase). Indeed, “Shuiguo” exhibited much better antioxidant activities and inhibitory activities on digestive enzymes, which might be attributed to its high TFC. Results suggested that okra, especially “Shuiguo”, could be developed as natural antioxidants and inhibitors against hyperlipidemia and hyperglycemia in the fields of functional foods and pharmaceuticals, which could meet the increasing demand for high-quality okra with health-promoting properties in China.

Antioxidant and free radical scavenging potential of crude sulphated polysaccharides from Turbinaria ornata

Biologia ◽  
2015 ◽  
Vol 70 (1) ◽  
Author(s):  
M. Manoj Saravana Guru ◽  
Mani Vasanthi ◽  
Anant Achary
Keyword(s):  
Free Radical ◽  
Superoxide Radical ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Antioxidant Power ◽  
Sulphated Polysaccharides ◽  
Turbinaria Ornata ◽  
Radical Scavenging Capacity ◽  
Scavenging Capacity ◽  
Total Antioxidant

AbstractThe sulphated polysaccharides from brown algae have been attracting extensive interest due to their numerous biological activities. The present study was designed to evaluate the antioxidant activity and free-radical scavenging capacity of crude sulphated polysaccharides (CSP) from Turbinaria ornata, a marine brown algae. The CSP was extracted from T. ornata using hot water with the yield of 14.31% (w/w). The chemical composition analysis of CSP showed 71.07 ± 4.61 % of total sugar, 1.81 ± 0.035 % total protein, 27 ± 1.49 % sulphate, and 6.16 ± 0.36 % total phenol. The presence of sulphated polysaccharides in the CSP was confirmed by agarose gel electrophoresis. The in vitro antioxidant activity of CSP was evaluated using total antioxidant power assay and ferric reducing antioxidant power assay (FRAP). The results revealed that the total antioxidant capacity of CSP was 22.205 ± 0.875 (equivalent mg of ascorbic acid/g of CSP) and 0.556 ± 0.03 (mM of FeSO4/mg of CSP) FRAP value, respectively. The free-radical scavenging ability of CSP was demonstrated using 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS), 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical and superoxide radical. The capacity of the CSP to scavenge 50% of free radicals was found to be 88.71 ± 1.01, 440.07 ± 4.43 and 352 ± 4.58 μg/mL for ABTS, DPPH and superoxide radical, respectively. These results showed that the CSP from T. ornata possess antioxidant and free-radical scavenging capacity.

scholarly journals Chemopreventative Potential of Plant-Based Extracts Coffee (Coffea) and Mauby (Colubrina Arborescens) using the Global Antioxidant Response (GAR) Method via in vitro Simulated Digestion

2021 ◽  
Vol 10 (3) ◽  
pp. 43
Author(s):  
Hannah Gross ◽  
Cheryl Rock ◽  
Yada Treesukosol ◽  
Virginia Gray
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Antioxidant Response ◽  
Free Radical Scavenging ◽  
Antioxidant Power ◽  
Simulated Digestion ◽  
Radical Scavenging Capacity ◽  
Scavenging Capacity

Plant-based extracts such as coffee (coffea) and Mauby (Colubrina Arborescens) were tested for chemopreventative potential by measuring their antioxidant activity (i.e., reducing power and free radical scavenging capacity) conventionally using chemical assays 1, 1-Diphenyl-2-picryl-hydrazyl (DPPH) and Ferric Reducing Antioxidant Power (FRAP). Alternately, extracts were also analyzed for their chemopreventative potential via a novel method (Global Antioxidant Response [GAR]), where they were subjected to simulated digestion before their antioxidant activity was determined via conventional methods previously mentioned. Next, the antioxidant capacities of the extracts, conventional versus the novel (GAR) method were compared. Overall, the analysis indicated that the reducing power (FRAP) and free radical scavenging capacity (DPPH) of coffee and Mauby were reduced (~16%) after undergoing simulated digestion. It was also observed that while the antioxidants in Mauby scavenged radicals at a significantly higher capacity than those in coffee (95.7% ± 0.67 and 90% ± 2.1 before digestion, and 77.7% ± 2.2 and 74.6% ± 2.3 after digestion, respectively), antioxidants in coffee exhibited higher reducing power compared to those in Mauby. Specifically, after undergoing simulated digestion, 1.12 mM FeSO4/mL ± 0.05 to 0.68 mM FeSO4/mL ± 0.07 ions were reduced before digestion, and 0.73 mM FeSO4/mL ± 0.09 to 0.48 mM FeSO4/mL ± 0.04 ions were reduced after digestion for coffee and Mauby, respectively. These findings suggest that while the antioxidants in coffee may have been more powerful in their ability to reduce ions, the antioxidants in Mauby may have been more effective in scavenging and neutralizing radicals.

scholarly journals Volatile Variability and Antioxidant Activity of Rosmarinus officinalis Essential Oil as Affected by Elevation Gradient and Vegetal Associations

2019 ◽  
Vol 31 (6) ◽  
pp. 1279-1288 ◽  
Author(s):  
M. SABBAHI ◽  
A. EL-HASSOUNI ◽  
A. TAHANI ◽  
A. EL-BACHIRI
Keyword(s):  
Antioxidant Activity ◽  
Essential Oil ◽  
Elevation Gradient ◽  
Radical Scavenging ◽  
Rosmarinus Officinalis ◽  
Gas Chromatography Mass Spectrometry ◽  
Dpph Assay ◽  
Radical Scavenging Capacity ◽  
Scavenging Capacity ◽  
Essential Oil Yield

The essential oil yield, volatile profile and antioxidant activity, of 37 samples collected from the Rosmarinus officinalis shrubs growing wild in the different ecosystems of the provinces of Taourirt and Jerada (Oriental region in the east of Morocco), were studied in order to determine the effect of the habitat and how the vegetal associations are influencing the volatile variability of their essential oil. The essential oil compositions were determined by gas chromatography-mass spectrometry (GC-MS). The yield of the essential oil extraction ranged from 1.09 to 2.81 %. The major components found were 1.8-cineol (37.71-65.02 %), camphor (6.09-27.49 %), α-pinene (3.08-9.98 %) and β-pinene (2.83-11.7 %). The interpretation of these results revealed that the variability is attributed to varieties and genetic background rather than the ecosystems. The antioxidant activity was determined by 1,1-diphenyl-1-picrylhydrazyl (DPPH) assay which revealed a strong radical scavenging capacity for all the samples with some variability which can be explained by the variability of the content of monoterpenes oxygenated.

Lignicolous fungi from northern Serbia as natural sources of antioxidants

2009 ◽  
Vol 4 (3) ◽  
pp. 387-396 ◽  
Author(s):  
Maja Karaman ◽  
Neda Mimica-Dukic ◽  
Milan Matavuly
Keyword(s):  
Radical Scavenging ◽  
Natural Antioxidants ◽  
Fungal Species ◽  
Oh Radicals ◽  
Lignicolous Fungi ◽  
Food Industries ◽  
Radical Scavenging Capacity ◽  
Scavenging Capacity ◽  
Tlc Analysis

AbstractAs a result of an interest in natural derived metabolites, lignicolous fungi have taken on great importance in biochemical investigations. In the present study, antioxidative screening analyses have included in vitro testing of different extracts (aqueous, methanol, chloroform) of four fungal species using three different assays: Fe2+/ascorbate-induced lipid peroxidation by TBA assay, the neutralisation of OH· radicals and the radical scavenging capacity with the DPPHk]assay. TLC analysis confirmed the existance of phenolics in the extracts, but also indicates the presence of some other compounds. The obtained results indicate that MeOH extracts manifested a degree of activity higher than that of CHCl3 extracts. With respect to antioxidative activity, the extracts can be ranged in the following declining order: G. lucidum, G. applanatum, M. giganteus and F. velutipes. These results suggest that analyzed fungi are of potential interest as sources of strong natural antioxidants that could be used in the food industries and nutrition.

scholarly journals Chemical composition, antimicrobial and antioxidant properties of Mentha longifolia (L.) Huds. essential oil

2012 ◽  
Vol 2 (3) ◽  
pp. 192-200 ◽  
Author(s):  
Haris Nikšić ◽  
Elvira Kovač Bešović ◽  
Elma Makarević ◽  
Kemal Durić
Keyword(s):  
Antimicrobial Activity ◽  
Essential Oil ◽  
Essential Oils ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Gas Chromatography Mass Spectrometry ◽  
Radical Scavenging Capacity ◽  
Scavenging Capacity ◽  
Mentha Longifolia ◽  
Layer Chromatography

Introduction: Present study describes the antimicrobial activity and free radical scavenging capacity (RSC) of essential oil from Mentha longifolia (L.) Huds. Aim of this study to investigate the quality, antimicrobial andantioxidant activity of wild species Mentha longifolia essential oil from Bosnia and Herzegovina.Methods: The chemical profi le of essential oil was evaluated by the means of gas chromatography-mass spectrometry (GC-MS) and thin-layer chromatography (TLC). Antimicrobial activity was tested against 6bacterial strains. RSC was assessed by measuring the scavenging activity of essential oils on 2,2- diphenyl-1-picrylhydrazil (DPPH).Results: The main constituents of the essential oil of M. longifoliae folium were oxygenated monoterpenes,piperitone oxide (63.58%) and 1,8-cineole (12.03%). Essential oil exhibited very strong antibacterial activity.The most important antibacterial activity essential oil was expressed on Gram negative strains: Escherichia coli, Pseudomonas aerginosa and Salmonella enterica. subsp.enterica serotype ABONY. Antioxidant activity was evaluated as a RSC. Investigated essential oil was able to reduce DPPH radicals into the neutral DPPHH form (IC50=10.5 μg/ml) and this activity was dose –dependent.Conclusion: The study revealed signifi cant antimicrobial activity of the investigated essential oil. The examined oil exhibited high RSC, which was found to be in correlation to the content of mainly monoterpeneketones and aldehydes. These results indicate that essential oils could serve as safe antioxidant and antiseptic supplements in pharmaceuticals.

scholarly journals Ecotypic Variations Affected the Biological Effectiveness of Thymus daenensis Celak Essential Oil

2021 ◽  
Vol 2021 ◽  
pp. 1-12
Author(s):  
Fatemeh Elahian ◽  
Maryam Garshasbi ◽  
Zahra Mehri Asiabar ◽  
Neda Gholamian Dehkordi ◽  
Alireza Yazdinezhad ◽  
...  
Keyword(s):  
Folk Medicine ◽  
Radical Scavenging ◽  
Biological Properties ◽  
Minimal Bactericidal Concentration ◽  
Radical Scavenging Capacity ◽  
Scavenging Capacity ◽  
Biological Effectiveness ◽  
Antibacterial And Antifungal ◽  
Dose Dependent ◽  
Β Carotene

Thymus (Lamiaceae) is famous for its pharmacological properties. Thymus daenensis Celak (Avishan-e-denaee in Persian) is an endemic Thymus species in Iran and is traditionally used for its digestive, carminative, antitussive, antispasmodic, and expectorant attributes in folk medicine. Ecotypic oils were extracted and analyzed with the GC-MS. Their biological properties in terms of antimicrobial, antioxidant, and antigenotoxic activities were evaluated using the minimal inhibitory concentration, minimal bactericidal concentration, and DPPH, β-carotene, and comet assays. The GC-MS results for Thymus daenensis Celak oils revealed thymol (73.86%) and carvacrol (51.89%) as the most abundant components. Due to the results, reasonable bactericidal activity values range from 0.14 to 5.00 mg/ml, and fungicidal activity ranges from 0.17 to 0.58 mg/ml. The necessary oil free radical scavenging capacity (0.41–1.79 mg/ml), bleaching inhibitory activity (0.01–1.06 mg/ml), and genoprotective potential (1.04–7.78 mg/ml) indicated the dose-dependent activity. The results suggest that Thymus daenensis is an important antibacterial and antifungal bioresource. Additionally, the antioxidant and radical scavenging capacity suggests this species has a role as a natural preservative in oxidative diseases and in the prevention of food spoilage.

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