Hepatoprotective effect of Moringa oleifera leaves aquatic extract against lead acetate–induced liver injury in male Wistar rats

Background: Moringa oleifera is currently and rapidly gaining superfood status. Objective: This study evaluated the effect of Moringa oleifera leaves (MOLM) on haematological, biochemical and histopathology indices of rats fed with Vitamins and Minerals Deficient (VMD) diets. Methods: Twenty-five male Wistar rats (220-290g) were randomly assigned into 5 groups of 5 rats each. The control (normal rat chow), remaining four diets were VMD plus MOLM at 0, 3, 5 and 10%. Haematological, biochemical and histopathological statuses were evaluated after 28 days. Results: Non-significant increase in RBC was observed in rats fed with the MOLM diets compared to control. Rats on VMD diet had the lowest haemoglobin levels, whereas haemoglobin increased with MOLM. No significant differences were observed in haematocrit, MCH, MCHC, platelets, RDW and MPV in all the treatments and control group. However, VMD rats showed a decrease in haemoglobin, and an increase in WBC and platelets. Electrolytes and glucose for all the treatments and control were not significantly different, but, control and VMD10 diets rats showed significantly higher values for creatinine compared to MOLM diets; total protein and albumin increased with MOLM and were higher than in VMD and control rats. Serum activities of hepatic, cardiac and adrenal marker enzymes were observed to be significantly lower in MOLM rats compared to VMD and control. Mild congestion in VMD and VMD3 rats’ liver and diffused congestion in the kidney of VMD diet rats were observed. Conclusion: The significantly high increase in platelet count in the VMD treated rats, indicative of anemia and other physiological imbalances, highlights the effect of micronutrient (mineral and vitamins) deficiencies in the diet. There is also the possibility that the anti-nutrients factors present in Moringa oleifera prevented uptake and bioavailability of nutrients, since the MOLM did not undergo any processing that could have removed or reduced these antinutrients. Further studies are still needed on Moringa oleifera leaves to validate the bioavailability of nutrients from the plant.

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Hepatoprotective Effect of Citrus Sinensis Peel Extract Against Isoniazid and Rifampicin-induced Liver Injury in Wistar Rats

Majalah Obat Tradisional ◽

10.22146/mot.45762 ◽

2019 ◽

Vol 24 (3) ◽

pp. 197

Author(s):

Edward Kosasih ◽

Linda Chiuman ◽

I Nyoman Ehrich Lister ◽

Edy Fachrial

Keyword(s):

Liver Injury ◽

Citrus Sinensis ◽

Wistar Rats ◽

Histopathological Examination ◽

Ethanolic Extract ◽

Hepatoprotective Effect ◽

Control Groups ◽

Male Wistar Rats ◽

The Rich ◽

Peel Extract

Tuberculosis (TB) is one of the leading causes of death in developing countries. One of the problems in controlling TB disease is that most anti-tuberculosis drugs are hepatotoxic. Citrus sinensis peel extract is the rich source of secondary metabolites with high potential effectiveness as an antioxidant. In the present study, we evaluate the hepatoprotective effect of Citrus sinensis peel ethanolic extract (CSPEE) on isoniazid and rifampicin-induced liver injury in Wistar rats. Twenty five adult male Wistar rats were divided into 5 groups of 5 each : control, (INH+RIF) (50 mg/kg bw once a day for 14 days), (INH+RIF) + various dose of CSPEE (300, 450, 600 mg/kg bw). CSPEE was given orally once a day for 14 days followed by administration of INH + RIF suspension. The measurement of serum ALT and AST were carried out on the 15th day. Histopathologic examination of the liver was also performed. The Serum ALT and AST of the rats that induced with INH + RIF were increased significantly (P<0.001) compare to those of control groups, and the histopathologic slides showed steatosis, vacuolation and necrosis of hepatic cells. The serum ALT and AST in groups treated with CSPEE were not significantly different (p>0.05) with those of control groups. The serum ALT and AST and histopathological examination of the liver of the group that administered 600 mg/kg CSPEE were closest to normal rats. Citrus sinensis peel extract exhibits hepatoprotective effect on liver injury induced with INH + RIF in Wistar rats.

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Carbamazepine evoked reproductive toxicity in male Wistar rats: protective properties of Moringa oleifera leaves methanolic extract

Comparative Clinical Pathology ◽

10.1007/s00580-020-03169-x ◽

2020 ◽

Vol 29 (6) ◽

pp. 1179-1187

Author(s):

Ganiu Jimoh Akorede ◽

Suleiman Folorunsho Ambali ◽

Mikail Garba Hudu ◽

Mohammed M. Suleiman ◽

Kolawole Yusuf Suleiman ◽

...

Keyword(s):

Wistar Rats ◽

Moringa Oleifera ◽

Methanolic Extract ◽

Reproductive Toxicity ◽

Protective Properties ◽

Male Wistar Rats ◽

Moringa Oleifera Leaves

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Effect of Citrullus lanatus Seeeds and Moringa oleifera Leaves on Glucose Level and Lipid Profile Parameters of Alloxan-Induced Diabetic Wistar Rats

International Journal of Innovative Research and Development ◽

10.24940/ijird/2017/v6/i12/dec17008 ◽

2017 ◽

Vol 6 (12) ◽

Author(s):

Ngaski A. A. ◽

Maryam Muhammad ◽

Mainasara A. S. ◽

Nasiru Sulaiman ◽

Muhammad Kabiru Dallatu ◽

...

Keyword(s):

Lipid Profile ◽

Glucose Level ◽

Wistar Rats ◽

Moringa Oleifera ◽

Citrullus Lanatus ◽

Moringa Oleifera Leaves

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Cocos nucifera L. oil alleviates lead acetate-induced reproductive toxicity in sexually-matured male Wistar rats

Journal of Basic and Clinical Physiology and Pharmacology ◽

10.1515/jbcpp-2020-0281 ◽

2021 ◽

Vol 0 (0) ◽

Author(s):

Olugbemi T. Olaniyan ◽

Olakunle A. Ojewale ◽

Ayobami Dare ◽

Olufemi Adebayo ◽

Joseph E. Enyojo ◽

...

Keyword(s):

Luteinizing Hormone ◽

Lead Acetate ◽

Wistar Rats ◽

Histological Analysis ◽

Cocos Nucifera ◽

Reproductive Toxicity ◽

Positive Control ◽

Negative Control ◽

Male Wistar Rats ◽

Cocos Nucifera L

Abstract Objectives Lead primarily affects male reproductive functions via hormonal imbalance and morphological damage to the testicular tissue with significant alteration in sperm profile and oxidative markers. Though, different studies have reported that Cocos nucifera L. oil has a wide range of biological effects, this study aimed at investigating the effect of Cocos nucifera L. oil on lead acetate-induced reproductive toxicity in male Wistar rats. Methods Twenty (20) sexually matured male Wistar rats (55–65 days) were randomly distributed into four groups (n=5). Group I (negative control)—distilled water orally for 56 days, Group II (positive control)—5 mg/kg bwt lead acetate intraperitoneally (i.p.) for 14 days, Group III—6.7 mL/kg bwt Cocos nucifera L. oil orally for 56 days and Group IV—lead acetate intraperitoneally (i.p.) for 14 days and Cocos nucifera L. oil for orally for 56 days. Rats were sacrificed by diethyl ether, after which the serum, testis and epididymis were collected and used for semen analysis, biochemical and histological analysis. Results The lead acetate significantly increases (p<0.05) testicular and epididymal malondialdehyde (MDA) levels, while a significant reduction (p<0.05) in sperm parameters, organ weight, testosterone and luteinizing hormone was observed when compared with the negative control. The coadministration of Cocos nucifera oil with lead acetate significantly increases (p<0.05) testosterone, luteinizing hormone, sperm parameters and organ weight, with a significant decrease (p<0.05) in MDA levels compared with positive control. Histological analysis showed that lead acetate distorts testicular cytoarchitecture and germ cell integrity while this was normalized in the cotreated group. Conclusions Cocos nucifera oil attenuates the deleterious effects of lead acetate in male Wistar rats, which could be attributed to its polyphenol content and antioxidant properties.

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Anticarcinogenic effect of probiotic fermented milk and chlorophyllin on aflatoxin-B1-induced liver carcinogenesis in rats

British Journal Of Nutrition ◽

10.1017/s0007114511003953 ◽

2011 ◽

Vol 107 (7) ◽

pp. 1006-1016 ◽

Cited By ~ 46

Author(s):

M. Kumar ◽

V. Verma ◽

R. Nagpal ◽

A. Kumar ◽

P. V. Behare ◽

...

Keyword(s):

Wistar Rats ◽

Lactobacillus Rhamnosus ◽

Thiobarbituric Acid ◽

Fermented Milk ◽

Hepatoprotective Effect ◽

Control Group ◽

Time Interval ◽

Group I ◽

Male Wistar Rats ◽

Aflatoxin B

The present investigation was carried out to evaluate the hepatoprotective effect of probiotic fermented milk (FM) containing Lactobacillus rhamnosus GG and Lactobacillus casei strain Shirota, alone as well as in combination with chlorophyllin (CHL) as an antioxidant agent in male Wistar rats administered aflatoxin-B1 (AFB1). AFB1 was injected intraperitoneally at the rate of 450 μg/kg body weight per animal twice a week for 6 weeks, maintaining an equal time interval between the two consecutive AFB1 administrations. A total of 125 male Wistar rats were randomly allocated to five groups, each group having twenty-five animals. Group I was offered FM containing L. rhamnosus GG and L. casei strain Shirota. Group II was administered AFB1 and served as the control group; group III was administered FM-AFB1, in which besides administering AFB1, FM was also offered. Group IV was offered CHL and AFB1, and group V was offered both FM and CHL along with AFB1. The rats were euthanised at the 15th and 25th week of the experiment and examined for the biochemical and hepatopathological profile. A significant reduction in thiobarbituric acid-reactive substances (TBARS) was observed in the FM–CHL–AFB1 group compared with the AFB1 control group. FM alone or in combination with CHL was found to show a significant (P < 0·05) hepatoprotective effect by lowering the levels of TBARS and by enhancing the activities of antioxidant enzymes such as glutathione peroxidase, superoxide dismutase, catalase and glutathione-S-transferase, indicating that probiotic FM alone or in combination with CHL possesses a potent protective effect against AFB1-induced hepatic damage.

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