Caffeic and chlorogenic acids inhibit key enzymes linked to type 2 diabetes (in vitro): a comparative study

AbstractChlorogenic acid is a major phenolic compound that forms a substantial part of plant foods and is an ester of caffeic acid and quinic acid. However, the effect of the structures of both chlorogenic and caffeic acids on their antioxidant and antidiabetic potentials have not been fully understood. Thus, this study sought to investigate and compare the interaction of caffeic acid and chlorogenic acid with α-amylase and α-glucosidase (key enzymes linked to type 2 diabetes) activities in vitro.The inhibitory effect of the phenolic acids on α-amylase and α-glucosidase activities was evaluated. Thereafter, their antioxidant activities as typified by their 1,1-diphenyl-2 picrylhydrazyl radical scavenging ability and ferric reducing antioxidant properties were determined.The results revealed that both phenolic acids inhibited α-amylase and α-glucosidase activities in a dose-dependent manner (2–8 μg/mL). However, caffeic acid had a significantly (p<0.05) higher inhibitory effect on α-amylase [ICThe esterification of caffeic acid with quinic acid, producing chlorogenic acid, reduces their ability to inhibit α-amylase and α-glucosidase activities. Thus, the inhibition of α-amylase and α-glucosidase activities by the phenolic acids could be part of the possible mechanism by which the phenolic acids exert their antidiabetic effects.

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Phenolic Acids from Lycium barbarum Leaves: In Vitro and In Silico Studies of the Inhibitory Activity against Porcine Pancreatic α-Amylase

Processes ◽

10.3390/pr8111388 ◽

2020 ◽

Vol 8 (11) ◽

pp. 1388

Author(s):

Luna Pollini ◽

Alessandra Riccio ◽

Cristina Juan ◽

Carmela Tringaniello ◽

Federica Ianni ◽

...

Keyword(s):

Phenolic Acids ◽

Inhibitory Activity ◽

Leaf Extract ◽

Inhibitory Effect ◽

Dependent Manner ◽

Lycium Barbarum ◽

Plant Materials ◽

Vitro Assay ◽

In Silico Studies

Nowadays, bioactive compounds from vegetable food and waste are of great interest for their inhibitory potential against digestive enzymes. In the present study, the inhibitory activity of methanolic extract from Lycium barbarum leaves on porcine pancreas α-amylase has been studied. The α-amylase inhibitory activity of the constituent phenolic acids was also investigated. The leaves were extracted by ultrasound-assisted method, one of the most efficient techniques for bioactive extraction from plant materials, and then the phenolic acids were identified by Accurate-Mass Quadrupole Time-of-Flight (Q-TOF) Liquid Chromatography/Mass Spectrometry (LC/MS). Chlorogenic and salicylic acids were the most abundant phenolic acids in L. barbarum leaf extract. The inhibitory effect against α-amylase, determined for individual compounds by in vitro assay, was higher for chlorogenic, salicylic, and caffeic acids. L. barbarum leaf extract showed an appreciable α-amylase inhibitory effect in a concentration-dependent manner. Docking studies of the considered phenolic acids into the active site of α-amylase suggested a conserved binding mode that is mainly stabilized through H-bonds and π-π stacking interactions.

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In vitro studies of eggplant (Solanum melongena) phenolics as inhibitors of key enzymes relevant for type 2 diabetes and hypertension

Bioresource Technology ◽

10.1016/j.biortech.2007.06.035 ◽

2008 ◽

Vol 99 (8) ◽

pp. 2981-2988 ◽

Cited By ~ 194

Author(s):

Y.-I. Kwon ◽

E. Apostolidis ◽

K. Shetty

Keyword(s):

Type 2 Diabetes ◽

Solanum Melongena ◽

In Vitro Studies ◽

Key Enzymes

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Inhibitory effect of polyphenolic–rich extract from Cola nitida (Kolanut) seed on key enzyme linked to type 2 diabetes and Fe2+ induced lipid peroxidation in rat pancreas in vitro

Asian Pacific Journal of Tropical Biomedicine ◽

10.12980/apjtb.4.2014c75 ◽

2014 ◽

Vol 4 ◽

pp. S405-S412 ◽

Cited By ~ 9

Author(s):

Ganiyu Oboh ◽

Kate E. Nwokocha ◽

Ayodele J. Akinyemi ◽

Adedayo O. Ademiluyi

Keyword(s):

Type 2 Diabetes ◽

Lipid Peroxidation ◽

Inhibitory Effect ◽

Rat Pancreas ◽

Key Enzyme ◽

Cola Nitida

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Inhibitory effect of polyphenol-rich extracts of jute leaf (Corchorus olitorius) on key enzyme linked to type 2 diabetes (α-amylase and α-glucosidase) and hypertension (angiotensin I converting) in vitro

Journal of Functional Foods ◽

10.1016/j.jff.2012.02.003 ◽

2012 ◽

Vol 4 (2) ◽

pp. 450-458 ◽

Cited By ~ 115

Author(s):

Ganiyu Oboh ◽

Adedayo O. Ademiluyi ◽

Ayodele J. Akinyemi ◽

Thomas Henle ◽

Jamiyu A. Saliu ◽

...

Keyword(s):

Type 2 Diabetes ◽

Inhibitory Effect ◽

Corchorus Olitorius ◽

Angiotensin I ◽

Key Enzyme

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Meteorin-like (Metrnl) adipomyokine improves glucose tolerance in type 2 diabetes via AMPK pathway

10.1101/420489 ◽

2018 ◽

Author(s):

Jung Ok Lee ◽

Hye Jeong Lee ◽

Yong Woo Lee ◽

Jeong Ah Han ◽

Min Ju Kang ◽

...

Keyword(s):

Type 2 Diabetes ◽

Glucose Metabolism ◽

Glucose Tolerance ◽

Underlying Mechanism ◽

Dependent Manner ◽

Beneficial Effects ◽

Ampk Pathway

AbstractMeteorin-like (metrnl) is a recently identified adipomyokine that has beneficial effects on glucose metabolism. However, its underlying mechanism of action is not completely understood. In this study, we have shown that a level of metrnl increase in vitro under electrical-pulse-stimulation (EPS) and in vivo in exercise mice, suggesting that metrnl is an exercise-induced myokine. In addition, metrnl increases glucose uptake through the calcium-dependent AMPK pathway. Metrnl also increases the phosphorylation of HDAC5, a transcriptional repressor of GLUT4, in an AMPK-dependent manner. Phosphorylated HDAC5 interacts with 14-3-3 proteins and sequesters them in the cytoplasm, resulting in the activation of GLUT4 transcription. The intraperitoneal injection of recombinant metrnl improves glucose tolerance in mice with high fat-induced obesity or type 2 diabetes (db/db), but this is not seen in AMPK β1β2 muscle-specific null mice (AMPK β1β2 MKO). In conclusion, we have demonstrated that metrnl induces beneficial effects on glucose metabolism via AMPK and is a promising therapeutic candidate for glucose-related diseases such as type 2 diabetes.

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